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Penicillium dermatophilum and application thereof

A penicillium and multipurpose technology, applied in the field of Penicillium dermatitis Pc-46 strain, can solve the problems of less spore production, greatly increase, and slow growth rate of Monascus, and achieve large spore production, simple cultivation, and rich The effect of the strain

Active Publication Date: 2019-01-11
ZHEJIANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the slow growth rate and low spore production of Monascus, it is difficult to increase the production of Lovastatin significantly

Method used

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  • Penicillium dermatophilum and application thereof
  • Penicillium dermatophilum and application thereof
  • Penicillium dermatophilum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1. Isolation, screening and identification of lovastatin-producing Penicillium crustosum (Penicillium crustosum) Pc-46 strain

[0033] 1. Medium

[0034] (1) PDA medium: 20% potato, 2% glucose, 2.0% agar, pH 5.5-6.0. For the isolation, purification and identification of Penicillium strains.

[0035] (2) Seed culture solution: 20% potato, 2% glucose, 0.5% beef extract, pH 5.0-5.5. Used for primary seed culture of various strains of Penicillium.

[0036] (3) Fermentation medium: 20% potato, 2% glucose, 1% glycerin, 0.5% beef extract, pH 5.0-5.5. It is used for the fermentation and cultivation of various strains of Penicillium, and the strains with high production of Lovastatin are screened.

[0037] 2. Experimental method

[0038] 2.1 Isolation and purification of Penicillium strains

[0039](1) The separation and purification method of Penicillium strains in soil: Take 1 g of soil sample and add it to 99 mL of sterile water, and shake it on a shaker at 120 ...

Embodiment 2

[0065] Embodiment 2, method for producing Lovastatin by fermentation of Penicillium dermis (P.crustosum) Pc-46 strain and identification of Lovastatin

[0066] 1 Strain: Penicillium dermis (P.crustosum) Pc-46 strain.

[0067] 2 media

[0068] (1) PDA medium: 20% potato, 2% glucose, 2.0% agar, pH 5.0-5.5. For the activation culture of Penicillium Pc-46 strain.

[0069] (2) PD primary seed culture solution: 20% potato, 2% glucose, pH 5.0-5.5. Used for primary seed culture of Penicillium Pc-46 strain.

[0070] (3) Secondary seed culture solution: 20% potato, 2% glucose, 1% glycerin, 0.5% peptone, 0.5% beef extract, pH 5.0-5.5. Used for secondary seed culture of Penicillium Pc-46 strain.

[0071] (4) Fermentation medium: glucose 12%, glycerin 4%, peptone 1%, beef extract 0.5%, MgSO 4 ·7H 2 O0.1%, NaCl 0.2%, KH 2 PO 4 0.1%, water 1L, pH 5.0-5.5. Used for the fermentation of Penicillium Pc-46 strain to produce Lovastatin.

[0072] 3 Experimental methods

[0073] 3.1 Cul...

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Abstract

The invention belongs to the field of microbial biotechnology, and relates to a strain of Penicillium dermatophilum (P. crustosum) Pc-46 for producing Lovastatin by liquid fermentation. Specifically,the invention discloses a Pc-46 Penicillium crustosum Pc-46 (deposit No.: CCTCC NO: M 2018343), and also discloses an application of the Penicillium dermatophilum use and fermentation production method. Specifically, the present invention discloses Penicillium dermatophilum (P. crustosum)Pc-46 for preparing lovastatin; and the yield of Lovastatin is about 155mg / L to 158mg / L.

Description

technical field [0001] The invention belongs to the field of microbial biotechnology, and relates to a P.crustosum Pc-46 strain for producing Lovastatin by liquid fermentation, a use and a fermentation production method. Background technique [0002] Penicillium fungi are important industrial microbial resources in my country, widely distributed in soil, vegetation, air, food and other habitats. In 1928, Fleming in the United Kingdom discovered penicillin from Penicillium, which completely changed the medical treatment of bacterial diseases and brought Penicillium fungi into people's field of vision. In 1976, Professor Akira Endo, a Japanese scholar, discovered the first statin in the fermentation product of Penicillium penicillium. Lovastatin (Lovastatin) is recognized as an ideal drug for lowering cholesterol in various countries. It has the characteristics of high efficiency, low toxicity and safety. It can selectively inhibit the activity of hydroxymethylglutaric acid c...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P17/06C12R1/80
CPCC12P17/06C12N1/145C12R2001/80
Inventor 许楚旋蒋冬花郑婕施吕梦霞王嘉琦
Owner ZHEJIANG NORMAL UNIVERSITY
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