Trichoderma spp. strain antagonizing maize stem rot and sheath blight and application thereof

A technology of corn stalk rot and Trichoderma, applied in the fields of application, fungicides, fungi, etc., can solve the problems of reduced corn yield and achieve the effect of fast growth and large spore production

Inactive Publication Date: 2014-01-01
上海大井生物工程有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In recent years, with changes in farming and cultivation systems, variety rotation, and large-scale planting of high-oil and high-starch varieties, the soil-borne diseases of maize have a tendency to increase year by year. The incidence rate is about 10-30% in general years. Reached more than 60%, resulting in a significant reduction in corn yield

Method used

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  • Trichoderma spp. strain antagonizing maize stem rot and sheath blight and application thereof
  • Trichoderma spp. strain antagonizing maize stem rot and sheath blight and application thereof
  • Trichoderma spp. strain antagonizing maize stem rot and sheath blight and application thereof

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Experimental program
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Effect test

Embodiment 1

[0020] Embodiment 1 is an in vitro confrontation antibacterial test, embodiments 2 to 4 are enzyme activity detection tests of antagonizing related enzymes, embodiment 5 is a live inoculation antibacterial effect test, and embodiments 6 to 7 are extraction of antibiotic secondary metabolites Analysis test.

[0021] Embodiment 1, Antibacterial test in vitro

[0022] Prepare a PDA plate, use a puncher to punch out 5mm diameter bacterial blocks from the edge of the colonies of Trichoderma and 3 strains of pathogenic bacteria (Fusarium moniliforme, Fusarium graminearum, and Rhizoctonia solani) cultured for 3 days, respectively. Transplant in the center of the two opposite sides of the plate with a distance of about 5 cm, invert culture at a constant temperature of 28°C, and repeat 3 times. A plate inoculated with only pathogenic bacteria was used as a control. 144h after inoculation, the straight-line growth distance and S ck , calculate the inhibition rate according to the fo...

Embodiment 2

[0026] Embodiment 2, Chitinase enzyme activity assay

[0027] The Trichoderma strain was inoculated on a PDA medium plate (diameter 90 mm), and cultured in a light constant temperature and humidity incubator at 25°C and 60% humidity for 5 days. Soak the plate covered with Trichoderma in sterilized water for 3-5 minutes, gently scrape and wash the spores and mycelium with a cotton swab, filter with absorbent cotton or 4 layers of gauze to remove the mycelium, and the filtrate is the spore suspension. Count according to the hemocytometer method, and the concentration of the diluted spore suspension is 10 6 pcs mL -1 Spore count.

[0028] Prepare 1 mg mL of N-acetylglucosamine (NAG) (chromatographically pure, Sigma Chemical Co.P.O., Germany) -1Take 6 test tubes of 25×250mm, add reagents according to Table 2, mix each tube evenly, cook in boiling water for 5 minutes, cool to room temperature immediately after taking out, then add 21.5mL distilled water to each test tube, shak...

Embodiment 3

[0037] Example 3 , β-1,3-glucanase enzyme activity detection

[0038] Glucose standard curve was made with reference to Yang Yanhong’s method (Yang Yanhong. Screening of hyperparasites of Huashan pine rust pathogen and preliminary study on its mechanism of action. Kunming: Southwest Forestry University, 2004), adding reagents according to Table 4, and taking 1mg / mL standard Glucose solution 0, 0.1, 0.2, 0.3, 0.4, 0.5mL, then add distilled water to make up to 2mL, then add 0.75mL DNS solution and mix well, react in boiling water bath for 15min, take it out immediately and put it in ice water bath Cool to room temperature, add 5mL of distilled water and mix well. The light absorption value was measured at 540nm with a 722-type spectrophotometer, and the standard curve was drawn with the concentration of the standard glucose solution as the abscissa and the corresponding light absorption value as the ordinate.

[0039] Table 4 Reagents for Establishing Glucose Standard Curve ...

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Abstract

The invention relates to a Trichoderma strain for antagonizing maize stem rot and banded sclerotial blight and application thereof, belonging to the field of biological control. The Trichoderma strain is Trichoderma atroviride SG3403 CGMCC No.6479. The invention also relates to application of the Trichoderma strain in preparing pesticides for controlling maize stem rot and banded sclerotial blight. The Trichoderma strain provided by the invention has the advantages of high growth speed and high spore production amount, the in-vitro standoff pathogen inhibition rates are respectively up to 71.91%, 74.77% and 73.11%, the enzyme activity of chitinase is 4.1452U, the enzyme activity of beta-1,3-glucanase is 0.5969U, and the enzyme activity of extracellular proteinase is 2.4545U. The strain provided by the invention can be used for producing biological pesticides for efficiently and quickly controlling maize stem rot and banded sclerotial blight.

Description

technical field [0001] The invention belongs to the field of biological control and relates to a Trichoderma strain and its application, in particular to a Trichoderma strain antagonizing corn stalk rot and sheath blight and its application. Background technique [0002] In recent years, with changes in farming and cultivation systems, variety rotation, and large-scale planting of high-oil and high-starch varieties, the soil-borne diseases of maize have a tendency to increase year by year. The incidence rate is about 10-30% in general years. Reaching more than 60%, resulting in a significant reduction in corn yield. Therefore, soil-borne diseases have become one of the main restrictive factors for the stable and high yield of maize. There are four main soil-borne diseases of maize in my country, namely stalk rot, head smut, sheath blight and take-all, among which stalk rot and sheath blight are particularly serious. [0003] Corn stalk rot (Corn Stalk Rot), also known as c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
Inventor 孙瑞艳陈捷曹磊刘志诚李雅乾姚丽美林振亚
Owner 上海大井生物工程有限公司
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