2results about How to "High sporulation" patented technology

This invention belongs to the field of edible fungi technology, and particularly relates to a sessile Ganoderma lucidum strain GT1 and its applications. A sessile Ganoderma lucidum strain ( Ganodermasessile GT1, deposited at the China Center for Type Culture Collection (CCTCC), accession number CCTCC NO: M 2026405. This invention utilizes perennial, high-yield fungal grass to replace sawdust in the cultivation of edible and medicinal fungi, and screens suitable fungal grass formulas for Ganoderma lucidum cultivation, achieving high yield and high quality of Ganoderma lucidum, which is of great significance for the sustainable development of the Ganoderma lucidum industry. This invention optimizes the growth environment and substrate selection to achieve large-scale cultivation of edible and medicinal fungi, providing resource and technical support for in-depth research and application.

This invention relates to the field of agricultural microbiology, and discloses a highly effective fungal agent for controlling ginger stem base rot and its preparation method. The agent includes *Trichoderma harzianum* (accession number CCTCC NO:M 20253070), which is first inoculated onto potato dextrose agar to obtain plates covered with fresh spores. Then, wheat bran and soybean meal are mixed to adjust the initial moisture content and initial pH to prepare a solid fermentation substrate. A diluted spore suspension is uniformly inoculated into the substrate for fermentation to obtain a fermentation product. The fermentation product is mixed with an extract and shaken, then filtered through sterile gauze to obtain a crude spore solution, which is finally diluted to obtain the final product. This invention achieves control by using *Trichoderma harzianum* to antagonize the pathogen and disrupt its cell structure. The use of wheat bran and soybean meal to construct a microenvironment with a high carbon-to-nitrogen ratio yields a fermentation product with high spore production. The combination of extract elution and gauze filtration to intercept residues and prepare a crude spore solution improves the purity of the fungal agent.