11results about How to "High sporulation" patented technology

The invention discloses a metarhizium aureoviride strain YEC and application of the metarhizium aureoviride strain YEC in prevention and treatment of teak camel moths. The Metarhizium anisoviride YEC provided by the invention is separated from soil in Guangdong province, can infect mangrove forest pests, namely teak camel moths, and can have relatively high fatality rate and stiff insect rate on the teak camel moths within a short time. The metarhizium aureoviride strain YEC is a biological control strain with potential development and application value in control of mangrove forest pests.

The invention belongs to the technical field of biological prevention and control of plant pests, and relates to application of streptomyces sdu1201 and / or secondary metabolites in prevention and control of plant pests. Research finds that the streptomyces sp. Sdu1201 and the secondary metabolite thereof have the effect of preventing and treating tetranychus urticae and root-knot nematode.

The invention relates to a trichoderma asperellum and pseudomonas aeruginosa compound microbial agent as well as a preparation method and application thereof. The compound microbial agent is prepared from a pseudomonas aeruginosa solid fermentation product, a trichoderma asperellum solid fermentation product, an auxiliary agent and a synergist, the number of effective spores in the compound microbial agent is greater than 10 < 10 > CFU / g; the pseudomonas aeruginosa is preserved in the China General Microbiological Culture Collection Center (CGMCC) on September 13, 2022, and the preservation number of the pseudomonas aeruginosa is CGMCC No.25697; the trichoderma asperellum is preserved in the China General Microbiological Culture Collection Center (CGMCC) on August 9, 2023, and the preservation number of the trichoderma asperellum is CGMCC No. 40752. The compound microbial agent can effectively solve the problems of soil-borne diseases such as pepper phytophthora blight and continuous cropping obstacles in the prior art, and has an obvious growth promoting effect on melon and vegetable plants such as pepper.

The invention relates to the technical field of tea tree planting, in particular to a tea garden annual management method for effectively preventing and controlling tea cake disease. The invention provides a tea garden annual management method for effectively preventing and controlling tea cake disease. The tea garden annual management method comprises the following steps: respectively spraying a compound probiotic agent in March, May and July; a base fertilizer is applied in November. The annual management method for the tea garden has an obvious growth promoting effect on tea trees, and compared with other control groups, tender leaves of the tea trees are brighter green in color, buds are neater, and the length of the tender leaves and the number of the buds are obviously improved. The method also has an obvious prevention and treatment effect on tea cake disease, and the prevention and treatment effect is obviously superior to that of medicaments in the prior art.

The present invention relates to the field of biological control technology, in particular to Roberts green muscardine and its application in biological control and growth promotion. The present invention provides Roberts green muscardine and a method for using the same for biological control. The strain is a green muscardine with high toxicity and insecticidal function. The strain has a simple cultivation method, rapid growth, and a large spore production. The mortality rate of infected locusts can reach 100.00%, and it is non-toxic and non-polluting, will not damage the ecology, is not easy to produce drug resistance, and is harmless to humans. It can be widely used in the prevention and control of locusts.

The invention provides a method for improving spore production of natamycin producing bacteria, belongs to the technical field of streptomyces culture, and mainly provides an FtsZ gene, and the nucleotide sequence of the FtsZ gene is shown as SEQ ID NO.1; the FtsZ gene is applied to promotion of spore production of streptomyces gilvosporeus; the invention provides a genetically engineered bacterium for improving the sporulation quantity of streptomyces gilvosporeus. The genetically engineered bacterium is obtained by introducing an FtsZ gene and an erythromycin strong promoter ermEp into the streptomyces gilvosporeus through a carrier, a strain of streptomyces gilvosporeus F607-F is provided, the streptomyces gilvosporeus F607-F is preserved on October 28, 2024, and the preservation number of the streptomyces gilvosporeus F607-F is CCTCC M 20242352; the invention also provides a solid culture medium for promoting spore production of streptomyces gilvosporeus. The genetically engineered bacterium F607-F provided by the invention has the spore production advantage and can produce natamycin at high yield.

The invention discloses a Beauveria bassiana Bbzy2407 strain and application thereof, the strain is Beauveria bassiana, and the strain is classified and named as Beauveria bassiana; the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the preservation date is December 02, 2024; the preservation number is CGMCC (China General Microbiological Culture Collection Center) The beauveria bassiana Bbzy2407 provided by the invention is a biocontrol fungus capable of being used for preventing and controlling major pest Holotrichia crassipes larvae of agriculture and forestry plants, has the characteristics of strong pathogenicity to the Holotrichia crassipes larvae, safety to the environment and difficulty in causing drug resistance of pests, and can be used for biologically preventing and controlling the Holotrichia crassipes larvae.

This invention belongs to the field of edible fungi technology, and particularly relates to a sessile Ganoderma lucidum strain GT1 and its applications. A sessile Ganoderma lucidum strain ( Ganodermasessile GT1, deposited at the China Center for Type Culture Collection (CCTCC), accession number CCTCC NO: M 2026405. This invention utilizes perennial, high-yield fungal grass to replace sawdust in the cultivation of edible and medicinal fungi, and screens suitable fungal grass formulas for Ganoderma lucidum cultivation, achieving high yield and high quality of Ganoderma lucidum, which is of great significance for the sustainable development of the Ganoderma lucidum industry. This invention optimizes the growth environment and substrate selection to achieve large-scale cultivation of edible and medicinal fungi, providing resource and technical support for in-depth research and application.

The invention relates to the technical field of plant growth regulators, in particular to a high-yield corn cultivation method. The invention provides a high-yield corn cultivation method which comprises the following steps: (1) mixing corn with a coating agent for coating treatment, and then planting; (2) spraying a plant growth regulator in an elongation stage, a large bell mouth stage and a tasseling and spinning stage; (3) harvesting the corn in a full ripening stage. The high-yield cultivation method for the corn can effectively promote growth of the corn and remarkably improve the yield of the corn, and has the excellent technical effect of preventing and treating corn diseases and insect pests.

The invention discloses application and a method of over-expressed BbCDR2 gene in improving spore production, insect killing and stress resistance of beauveria bassiana. The nucleotide sequence of the BbCDR2 gene is shown as SEQ ID NO.5, and the amino acid sequence is shown as SEQ ID NO.6. The gene BbCDR2 is over-expressed in the beauveria bassiana, so that the spore production, insect killing and stress resistance of the beauveria bassiana are improved, and the yield of the beauveria bassiana is improved. The sporulation quantity and infection virulence of the beauveria bassiana can be improved, the spore wall damage and hypertonic stress resistance of the beauveria bassiana can be enhanced, and the method has fundamental significance in improving the performance and practical application of the beauveria bassiana strain.

This invention relates to the field of agricultural microbiology, and discloses a highly effective fungal agent for controlling ginger stem base rot and its preparation method. The agent includes *Trichoderma harzianum* (accession number CCTCC NO:M 20253070), which is first inoculated onto potato dextrose agar to obtain plates covered with fresh spores. Then, wheat bran and soybean meal are mixed to adjust the initial moisture content and initial pH to prepare a solid fermentation substrate. A diluted spore suspension is uniformly inoculated into the substrate for fermentation to obtain a fermentation product. The fermentation product is mixed with an extract and shaken, then filtered through sterile gauze to obtain a crude spore solution, which is finally diluted to obtain the final product. This invention achieves control by using *Trichoderma harzianum* to antagonize the pathogen and disrupt its cell structure. The use of wheat bran and soybean meal to construct a microenvironment with a high carbon-to-nitrogen ratio yields a fermentation product with high spore production. The combination of extract elution and gauze filtration to intercept residues and prepare a crude spore solution improves the purity of the fungal agent.