36results about How to "Rapid colonization" patented technology

The invention provides a bio-control bacterium foliage fertilizer, and preparation and application thereof. The bio-control bacterium foliage fertilizer is prepared from the following materials in parts by weight: 1-3 parts of core bio-control bacteria, 35-85 parts of biochemical fulvic acid potassium, 10-50 parts of active SiO2, 2-6 parts of an enzyme activity substance, and 2-6 parts of a plant surfactant. The core bio-control bacteria contain bio-control promoting bacteria and protein decomposing bacteria; the bio-control promoting bacteria comprise bacillus subtilis and trichoderma harzianum. The core bacteria in the bio-control bacteria foliage fertilizer can rapidly grow on the surface of a plant in a field planting manner and form a 'bio-control membrane' so as to prevent infection of pathogenic bacteria at the surface of the plant; the substances, such as antibiotics and phytohormone, are generated in the processes of interaction with the pathogenic bacteria and hyperparasitism, and a plurality of diseases of crops can be prevented, resisted or directly killed; the bio-control bacteria foliage fertilizer enables the crops to have solid plant cell wall tissue, so that the ability of plant epidermis for directly resisting incursion of the pathogenic bacteria and insect pests is enhanced, and healthy growth of the crops is facilitated; the target of using a little of pesticide or not is achieved, and the environmental pollution is alleviated. Thus, the yield and the quality of the crops are improved.

A microencapsulation method of triple probiotics relates to a cell immobilization biological method. The method comprises the following steps: firstly, culturing triple probiotics to a logarithmic growth phase, preparing a soybean protein bacterial suspension; secondly, mixing the soybean protein bacterial suspension with gelatin to prepare a bacterial gel solution; thirdly, mixing the bacterial gel solution with sodium alginate, performing emulsification; fourthly, sprinkling a calcium chloride solution, performing a capsulation reaction; fifthly, spraying chitosan, performing further solidification; sixthly, washing with a calcium chloride solution, performing centrifugation, collecting capsules. The invention can perform simultaneous microencapsulation of three probiotics; A probiotic preparation prepared by the invention has very strong stress resistance, and can be colonized and grow in intestinal tracts more rapidly.

The invention discloses a saline-alkali soil modified microbial inoculum and a preparation method thereof. The microbial inoculum comprises, by weight, 10-30 parts of bacillus megatherium HB bacteriapowder, 35-65 parts of phosphorus tailings powder, 12-18 parts of phosphogypsum, 8-25 parts of sugar refinery wastes, 6-26 parts of seaweed residue and 1-8 parts of mannitol. Components of the saline-alkali soil modified microbial inoculum have synergistic interactions with each other to enhance effects, if any one of the components lacks, expected effects cannot be reached, and only when the saline-alkali soil modified microbial inoculum with whole formulas is adopted and 30-80 kg of the microbial inoculum is used per mu, can the effects of increasing fertilizer utilization rate and crop yields, and improving quality and saline-alkali lands be achieved.

The invention relates to bacillus megaterium, a polypeptide agricultural microbial agent thereof as well as a preparation method thereof and applicationof the polypeptide agricultural microbial agent.The bacillus megaterium takes distillers' grains, soya bean meal and straw powder, which are rich in protein content, as main raw materials, and are mixed with corn flour, wheat bran, inorganic saltsand a few carbon source to reasonably compound and steam; high-yield protease bacillus subtilis is firstly utilized, the bacillus megaterium is utilized for decomposing different proteins in raw materials under a special fermentation condition to prepare the agricultural microbial agent which takes active peptides, amino acid, polysaccharides and the like as efficient ingredients; the microbial agent contains a great number of living spores, 17 free amino acids, a plurality of active peptides, and polysaccharides, and nutrient content is rich, the activity of thallus is high, and shelf life reaches two years or more; and according to the field experiment, the good effects of improving quality and increasing the yield are achieved.

The invention provides a biological organic fertilizer for preventing and treating root-knot nematode and a preparation method and application of the biological organic fertilizer. The biological organic fertilizer comprises 5-30 parts of a composite bacterial agent and 70-95 parts of a carrier, wherein the composite bacterial agent contains bacillus subtilis, paecilomyces lilacinus, pochonia chlamydospora and streptomycete, and the carrier is made from chicken manure, mushroom residues, and rape seed cake through microorganism compost fermentation. The composite bacterial agent has the effectof preventing and treating root-knot nematode in soil. The carrier is abundant in organic components and inorganic components, which can improve soil, increase the nutritive components in soil, accelerate the effective colonization of the composite bacterial agent in soil, and improve the capability of controlling treating root-knot nematode in soil of the composite bacterial agent. The providedbiological organic fertilizer for preventing and treating root-knot nematode is powder, can be used as a base fertilizer, is harmless to human and animals, is good in preventing and treating effect, and can obviously improve the quality and yield of crops.

The present invention discloses a nutritional composition for improving oral health. The nutritional composition comprises the following raw materials in parts by weight: 5-30 parts of probiotics, 20-80 parts of prebiotics and 0.1-2 parts of epigallocatechin gallate; the probiotics are selected from at least one of lactobacillus rhamnosus, lactobacillus reuteri, lactobacillus helveticus, and lactobacillus salivarius; and the prebiotics are selected from at least one of inulin, oligofructose, galactooligosaccharide, xylooligosaccharide, stachyose, oligoisomaltose, raffinose, trehalose, and lactitol. At the same time, the present invention also provides a use of the nutritional composition in preparations of oral health-care products. The provided nutritional composition can inhibit colonization of caries inducing streptococcus mutans, reduces adhesion of subgingival gingiva pathogenic bacteria, inhibits bad breath, promotes the proliferation of the oral probiotics, prevents the occurrence and development of dental erosion and oral cancers, and can effectively solve the oral problems caused by alcoholism, smoking, staying up late, irregular diet, long-term consumption of acidic substances, etc.

The invention discloses a probiotics preparation for conditioning infant intestinal tracts. The preparation is prepared by the following steps: taking walnut oil or plant blend oil taking walnut oil as a main component as a carrier, adds probiotics and other blend components into the carrier, performing blending at low temperature, and carries out negative-pressure deoxygenation to enable the probiotics to be embedded in the carrier so as to obtain drops. The method is simple, the process is easy to control, and the drops are stable in performance, do not solidify at low temperature, and are uniform in components. The viable bacteria in the probiotic drops are always in a dormant state, and the drops are stable after long-time preservation, light in taste, and natural and safe. After the drops are taken, the drops can withstand the acid and alkali environment in a human body, the survival rate of probiotics is high, the probiotics are rapidly planted and propagated in the intestinal tract of the human body, and the balance of microecology is adjusted, so that various intestinal tract problems are safely, effectively and efficiently solved. The method is suitable for preparing the probiotic preparation, and the preparation is further used for conditioning symptoms such as constipation and diarrhea in intestinal tracts of infants under the age of 3 years.

The invention discloses a bacillus safensis strain ZDC-02, and belongs to the technical field of biological control of plant diseases, wherein the strain is preserved in China General Microbiological Culture Collection Center with preservation number of CGMCC No.12217. Experiments indicate that the bacillus safensis strain ZDC-02 has the characteristics of being rapid in growth speed, broad in action spectrum, strong in stress resistance, capable of achieving rapid mass colonization on plant bodies and the like; therefore, the strain has a good application prospect. A bio-control preparation, as a bio-control preparation which has a quite broad application prospect and is prepared from the bacillus safensis, not only can effectively prevent and control various plant diseases but also can effectively improve a crop yield.

The invention discloses a polyglutamic acid gel micrococcus agent.A microorganism, a glycidyl methacrylate modified gama-polyglutamic acid, a photoinitiator, and water are used as dispersion phases, ahexadecane and a span 80 are used as continuous phases, droplet-based microfluidics technology is adopted to prepare microspheres, the sizes of themicrospheresare between 100 to 500 [mu]m. The invention further discloses preparation method and application of the polyglutamic acid gel micrococci agent. According to the polyglutamic acid gel microspheres,anembedding microbial agent can be released,the effective period of the high effective viable count can be prolonged, high activity biological function is realized, colonization of the functional microbial agent in the soil is advantageous, avariety of crop diseasescan be effectivelyprevented and controlled, the using amounts of chemical pesticides and chemical fertilizers are decreased, biological control and chemical control areeffectively combined, strengths are taken to offset weaknesses, integrated crop prevention and control is realized, and time and effort are saved.

The invention provides a water-soluble medicinal fertilizer containing a strong-antagonism anti-rice blast bacterial strain, a preparation method and application of the medicinal fertilizer. The medicinal fertilizer is prepared from the following constituents in parts by weight: 55-85 parts of an HR (Hypersensitive Response) antibiological inoculant, 10-30 parts of polyglutamic acid, and 5-15 parts of a plant cell stabilizer, wherein the HR antibiological inoculant comprises bacillus subtilis-w3 with an effective viable count of 7.0-9.0*10<9> cfu/g. The medicinal fertilizer disclosed by the invention can achieve a good biological control effect on rice blast, banded sclerotial blight and the like, significantly promotes growth of the rice, increases number of productive ears, setting percentage and thousand seed weight of the rice, and remarkably improves the vegetable yield and the quality.

The invention discloses a microbial fertilizer for improving soil, which is prepared from the following raw materials in percentage by weight: Bacillus mucilaginosus 40-45%; potassium fulvate 25-30%;diethyl aminoethyl hexanoate 2-3 %; yeast extract 5-6 %; chitosan oligosaccharide 5-6 %; sodium humate 4-6 %; seaweed extract 4-6 %; compound proteinase 2-3 %; Bacillus licheniformis 4-5 %. The invention can not only improve the soil, but also provide nutrition for the growth and development of plants and enhance the disease resistance of the plants through the mutual cooperation and mutual promotion among the components of the invention. Therefore, the invention can reduce the use amount of chemical fertilizers, improve the soil improvement effect and effectively degrade heavy metals and pesticides in the soil.

The invention relates to lactobacillus agilis and an application thereof and belongs to the technical field of microorganisms. The bacterial strain is a lactic acid bacterium which is separated from a normal microbial flora in intestinal tracts of a healthy pig. By means of Gram staining microscopy, physiological biochemical test and 16s-rRNA sequence analysis, the bacterial strain is identified to be a strain of lactobacillus agilis. Through growth curve drawing, gastrointestinal tract stress resistance environment test and in-vitro enteroadhesive test screening, the lactobacillus agilis strain shows the best biological characters when being compared with other lactic acid bacteria separated from the intestinal tract. The lactobacillus agilis can be applied as a feed additive in production of piglets when being completely cultured. The lactobacillus agilis can promote growth and increase utilization rate of the feed, can reduce diarrhea ratio, improve animal immunity level and improve disease resistance, can effective replace antibiotics in feed additives. The invention provides an excellent bacterial strain for probiotics, and has important significance of improving economic benefit in pig breeding and developing healthy breeding of pigs.

The invention discloses a probiotic drop for improving allergic constitution and a preparation method of the probiotic drop, and belongs to the technical field of medical biology. The probiotic drop comprises the following raw materials: lactobacillus casei, lactobacillus paracasei, lactobacillus rhamnosus, lactobacillus reuteri, lactobacillus acidophilus, bifidobacterium longum, lactobacillus plantarum, fermented lactic acid bacteria, galactooligosaccharide, xylooligosaccharide, anhydrous glucose, protein hydrolysate, polyhydric alcohols, natural vitamin E, unsaturated fatty acid, vegetable oil and phospholipid oil. The preparation method of the drop is simple, the process is easy to control, and the drop is stable in performance, not solidified at low temperature, uniform in component, good in oxidation resistance and more stable in storage.

The invention relates to the technical field of microorganisms, in particular to salt-tolerant bacillus and application thereof. The salt-tolerant bacillus strain BAH-1 provided by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the salt-tolerant bacillus strain BAH-1 is CGMCC No. 23503. The halotolerant bacillus BAH-1 is high in colonization capacity and breeding speed in plants, has the effect of efficiently inhibiting plant pathogenic bacteria, has the effect of simultaneously treating plant diseases such as red crown rot, brown spot and stem black spot disease, is high in control effect, has a very good application prospect in plant disease control, and has a wide application prospect. And a foundation is laid for the development of efficient and broad-spectrum biocontrol preparations.

The invention discloses a pepper seedling raising method suitable for winter and spring in northern areas, which belongs to the technical field of line pepper planting and comprises the following steps: (1) substrate preparation, (2) seed treatment, (3) germination accelerating treatment and (4) sowing and seedling raising. The method is simple in overall step and easy to popularize and apply, and the cultivated pepper seedlings are stable in quality and high in survival rate.

The invention relates to the technical field of calf breeding, in particular to a feed for calves and a calf raising method. Buffalo calves are bred by a voluntarily research and developed feed and raising method, quick colonization and propagation of probiotics in intestinal tracts of buffaloes can be accelerated, and the intestinal tract environment of the buffaloes can be improved. According tothe method, the buffaloes are promoted to eat more mainly through improving the mouth feel of the feed and increasing the probiotics; and the feed does not use hormones, so that the appetite and theimmunity of the buffaloes bred with the feed are better than those of the buffaloes raised by a conventional method, and the meat quality is better.

The invention relates to a water depth optimization and rapid recovery method of a Scirpus nipponicu wetland under saline-alkali stress. The method includes collecting Scirpus nipponicu corms for seedling raising, sowing the seedlings in different culture bowls, placing the seedlings in a water tank to build a water depth gradient, and adding a Hoagland semi-nutrient solution with different concentrations of NaHCO3+NaCl for culture; and after the culture, measuring the aboveground, underground and corm biomass of the Scirpus nipponicu, and analyzing the optimum water depth for the growth of the Scirpus nipponicu under different saline-alkali concentrations. The results show that when the concentration of saline-alkali is 0, the water depth is adjusted to 0cm; when the saline-alkali concentration is 2.5 g kg-1, the water depth is adjusted to 15-30cm; when the saline-alkali concentration is 5.00 g kg-1, the water depth is adjusted to 15cm; and when the saline-alkali concentration is larger than 10.0 g kg-1, the Scirpus nipponicu cannot survive. By studying the influence of water depth on the growth of the Scirpus nipponicu under different saline-alkali stress degrees, the optimal water depth for the growth of the Scirpus nipponicu under different saline-alkali concentrations is determined, the biomass accumulation of the Scirpus nipponicu is effectively improved, and the rapid colonization and recovery of Scirpus nipponicu population are facilitated.