36results about How to "Rapid colonization" patented technology

The invention provides a soluble straw decomposing agent, its preparation method and application. The soluble straw decomposing agent comprises by weight: 10-20 parts of a composite bacterial agent, 20-50 parts of nutrients, 2-5 parts of a plant source emulsification penetrant, and 25-68 parts of a soluble carrier. The composite bacterial agent contains cellulose decomposition bacteria, protein decomposition bacteria, and nutrient bio-control growth-promoting bacteria. The invention also provides a preparation method of the above soluble straw decomposing agent. The soluble straw decomposing agent provided in the invention is in a powder form and is fully soluble in water, and also can be applied through both scattering and spraying, thus being suitable for mechanized operation. The soluble straw decomposing agent provided in the invention can substantially speed up degradation of a plurality of crop straw, promote crop growth, and prevent a lot of crop diseases and pests.

The invention provides a bio-control bacterium foliage fertilizer, and preparation and application thereof. The bio-control bacterium foliage fertilizer is prepared from the following materials in parts by weight: 1-3 parts of core bio-control bacteria, 35-85 parts of biochemical fulvic acid potassium, 10-50 parts of active SiO2, 2-6 parts of an enzyme activity substance, and 2-6 parts of a plant surfactant. The core bio-control bacteria contain bio-control promoting bacteria and protein decomposing bacteria; the bio-control promoting bacteria comprise bacillus subtilis and trichoderma harzianum. The core bacteria in the bio-control bacteria foliage fertilizer can rapidly grow on the surface of a plant in a field planting manner and form a 'bio-control membrane' so as to prevent infection of pathogenic bacteria at the surface of the plant; the substances, such as antibiotics and phytohormone, are generated in the processes of interaction with the pathogenic bacteria and hyperparasitism, and a plurality of diseases of crops can be prevented, resisted or directly killed; the bio-control bacteria foliage fertilizer enables the crops to have solid plant cell wall tissue, so that the ability of plant epidermis for directly resisting incursion of the pathogenic bacteria and insect pests is enhanced, and healthy growth of the crops is facilitated; the target of using a little of pesticide or not is achieved, and the environmental pollution is alleviated. Thus, the yield and the quality of the crops are improved.

A microencapsulation method of triple probiotics relates to a cell immobilization biological method. The method comprises the following steps: firstly, culturing triple probiotics to a logarithmic growth phase, preparing a soybean protein bacterial suspension; secondly, mixing the soybean protein bacterial suspension with gelatin to prepare a bacterial gel solution; thirdly, mixing the bacterial gel solution with sodium alginate, performing emulsification; fourthly, sprinkling a calcium chloride solution, performing a capsulation reaction; fifthly, spraying chitosan, performing further solidification; sixthly, washing with a calcium chloride solution, performing centrifugation, collecting capsules. The invention can perform simultaneous microencapsulation of three probiotics; A probiotic preparation prepared by the invention has very strong stress resistance, and can be colonized and grow in intestinal tracts more rapidly.

The invention discloses a saline-alkali soil modified microbial inoculum and a preparation method thereof. The microbial inoculum comprises, by weight, 10-30 parts of bacillus megatherium HB bacteriapowder, 35-65 parts of phosphorus tailings powder, 12-18 parts of phosphogypsum, 8-25 parts of sugar refinery wastes, 6-26 parts of seaweed residue and 1-8 parts of mannitol. Components of the saline-alkali soil modified microbial inoculum have synergistic interactions with each other to enhance effects, if any one of the components lacks, expected effects cannot be reached, and only when the saline-alkali soil modified microbial inoculum with whole formulas is adopted and 30-80 kg of the microbial inoculum is used per mu, can the effects of increasing fertilizer utilization rate and crop yields, and improving quality and saline-alkali lands be achieved.

The invention relates to bacillus megaterium, a polypeptide agricultural microbial agent thereof as well as a preparation method thereof and applicationof the polypeptide agricultural microbial agent.The bacillus megaterium takes distillers' grains, soya bean meal and straw powder, which are rich in protein content, as main raw materials, and are mixed with corn flour, wheat bran, inorganic saltsand a few carbon source to reasonably compound and steam; high-yield protease bacillus subtilis is firstly utilized, the bacillus megaterium is utilized for decomposing different proteins in raw materials under a special fermentation condition to prepare the agricultural microbial agent which takes active peptides, amino acid, polysaccharides and the like as efficient ingredients; the microbial agent contains a great number of living spores, 17 free amino acids, a plurality of active peptides, and polysaccharides, and nutrient content is rich, the activity of thallus is high, and shelf life reaches two years or more; and according to the field experiment, the good effects of improving quality and increasing the yield are achieved.

The invention provides a biological organic fertilizer for preventing and treating root-knot nematode and a preparation method and application of the biological organic fertilizer. The biological organic fertilizer comprises 5-30 parts of a composite bacterial agent and 70-95 parts of a carrier, wherein the composite bacterial agent contains bacillus subtilis, paecilomyces lilacinus, pochonia chlamydospora and streptomycete, and the carrier is made from chicken manure, mushroom residues, and rape seed cake through microorganism compost fermentation. The composite bacterial agent has the effectof preventing and treating root-knot nematode in soil. The carrier is abundant in organic components and inorganic components, which can improve soil, increase the nutritive components in soil, accelerate the effective colonization of the composite bacterial agent in soil, and improve the capability of controlling treating root-knot nematode in soil of the composite bacterial agent. The providedbiological organic fertilizer for preventing and treating root-knot nematode is powder, can be used as a base fertilizer, is harmless to human and animals, is good in preventing and treating effect, and can obviously improve the quality and yield of crops.

The invention provides a bamboo carbon-polyvinyl formal biological carrier for water treatment and a process for preparing the same. The bamboo carbon-polyvinyl formal biological carrier is designed for solving the technical problems of high price, high abrasion ratio, large density, easy fragile, and difficult cleaning and replacement in the prior biological carrier active carbon and the inorganic carrier, and is produced by using polyvinyl formal as the organic polymer carrier, and adding filler such as bamboo-carbon for foaming treatment of calcium carbonate and citric acid. The prepared product has the characteristics of communication pore, multiple pores, micro pores, has large specific surface and biological compatibility, facilitates bacteria laminating and carries a large amount of bacteria, can fully meet the application requirements of suspended filler for water treatment, has great scientific and social significance, and can create good environmental, social and economic benefits if being popularized and applied.

The present invention discloses a nutritional composition for improving oral health. The nutritional composition comprises the following raw materials in parts by weight: 5-30 parts of probiotics, 20-80 parts of prebiotics and 0.1-2 parts of epigallocatechin gallate; the probiotics are selected from at least one of lactobacillus rhamnosus, lactobacillus reuteri, lactobacillus helveticus, and lactobacillus salivarius; and the prebiotics are selected from at least one of inulin, oligofructose, galactooligosaccharide, xylooligosaccharide, stachyose, oligoisomaltose, raffinose, trehalose, and lactitol. At the same time, the present invention also provides a use of the nutritional composition in preparations of oral health-care products. The provided nutritional composition can inhibit colonization of caries inducing streptococcus mutans, reduces adhesion of subgingival gingiva pathogenic bacteria, inhibits bad breath, promotes the proliferation of the oral probiotics, prevents the occurrence and development of dental erosion and oral cancers, and can effectively solve the oral problems caused by alcoholism, smoking, staying up late, irregular diet, long-term consumption of acidic substances, etc.

The invention discloses a probiotics preparation for conditioning infant intestinal tracts. The preparation is prepared by the following steps: taking walnut oil or plant blend oil taking walnut oil as a main component as a carrier, adds probiotics and other blend components into the carrier, performing blending at low temperature, and carries out negative-pressure deoxygenation to enable the probiotics to be embedded in the carrier so as to obtain drops. The method is simple, the process is easy to control, and the drops are stable in performance, do not solidify at low temperature, and are uniform in components. The viable bacteria in the probiotic drops are always in a dormant state, and the drops are stable after long-time preservation, light in taste, and natural and safe. After the drops are taken, the drops can withstand the acid and alkali environment in a human body, the survival rate of probiotics is high, the probiotics are rapidly planted and propagated in the intestinal tract of the human body, and the balance of microecology is adjusted, so that various intestinal tract problems are safely, effectively and efficiently solved. The method is suitable for preparing the probiotic preparation, and the preparation is further used for conditioning symptoms such as constipation and diarrhea in intestinal tracts of infants under the age of 3 years.

The invention discloses a bacillus safensis strain ZDC-02, and belongs to the technical field of biological control of plant diseases, wherein the strain is preserved in China General Microbiological Culture Collection Center with preservation number of CGMCC No.12217. Experiments indicate that the bacillus safensis strain ZDC-02 has the characteristics of being rapid in growth speed, broad in action spectrum, strong in stress resistance, capable of achieving rapid mass colonization on plant bodies and the like; therefore, the strain has a good application prospect. A bio-control preparation, as a bio-control preparation which has a quite broad application prospect and is prepared from the bacillus safensis, not only can effectively prevent and control various plant diseases but also can effectively improve a crop yield.

The invention discloses a polyglutamic acid gel micrococcus agent.A microorganism, a glycidyl methacrylate modified gama-polyglutamic acid, a photoinitiator, and water are used as dispersion phases, ahexadecane and a span 80 are used as continuous phases, droplet-based microfluidics technology is adopted to prepare microspheres, the sizes of themicrospheresare between 100 to 500 [mu]m. The invention further discloses preparation method and application of the polyglutamic acid gel micrococci agent. According to the polyglutamic acid gel microspheres,anembedding microbial agent can be released,the effective period of the high effective viable count can be prolonged, high activity biological function is realized, colonization of the functional microbial agent in the soil is advantageous, avariety of crop diseasescan be effectivelyprevented and controlled, the using amounts of chemical pesticides and chemical fertilizers are decreased, biological control and chemical control areeffectively combined, strengths are taken to offset weaknesses, integrated crop prevention and control is realized, and time and effort are saved.

The invention provides a water-soluble medicinal fertilizer containing a strong-antagonism anti-rice blast bacterial strain, a preparation method and application of the medicinal fertilizer. The medicinal fertilizer is prepared from the following constituents in parts by weight: 55-85 parts of an HR (Hypersensitive Response) antibiological inoculant, 10-30 parts of polyglutamic acid, and 5-15 parts of a plant cell stabilizer, wherein the HR antibiological inoculant comprises bacillus subtilis-w3 with an effective viable count of 7.0-9.0*10<9> cfu / g. The medicinal fertilizer disclosed by the invention can achieve a good biological control effect on rice blast, banded sclerotial blight and the like, significantly promotes growth of the rice, increases number of productive ears, setting percentage and thousand seed weight of the rice, and remarkably improves the vegetable yield and the quality.

The invention provides a preparation method and application of a biological seedling raising substrate of chaetomium globosum, and belongs to the technical field of agricultural microorganisms. The method comprises the steps that after preliminary fermentation is performed on agricultural waste, the agricultural waste is inoculated with chaetomium globosum ND35 sexual conidial powder to perform secondary fermentation, so that a functional flora taking chaetomium globosum as the main microorganisms in the substrate, and the biological seedling raising substrate of chaetomium globosum is obtained. The agricultural waste comprises, but is not limited to, bark, wood chips, straw and the like. The preparation method and application of the biological seedling raising substrate of chaetomium globosum have the advantages of being simple, being capable of being directly applied to the seedling raising stages of tomatoes, cucumbers and other plants, planting chaetomium globosum ND35 bacterial strains into the plants more directly and quickly, and being beneficial to the plants perpetually; the biological seedling raising substrate of chaetomium globosum is an excellent seedling raising substrate, can be applied to industrialized seedling raising of various plants, fills in the blank of domestic excellent substrates, replaces imported substrates, promotes the healthy development of industrialized seedling raising of vegetables, and has great economic and social benefits.

The invention relates to Streptomyces alboflavus, a method using the Streptomyces alboflavus to prepare a biocontrol microbial agent and application. The method includes: placing the Streptomyces alboflavus into a preservation culture medium, activating at 28-32 DEG C for 5-7 days, placing into a seed culture medium, and culturing at 30 DEG C and 160-180r / min for 36-48 hours to obtain a seed bacterium solution; inoculating the seed bacterium solution into a fermentation culture medium, performing fermentation culture in a constant-temperature culture tank of 30 DEG C for 7 days, placing the matrix obtained after the fermentation culture into a ventilation drying oven of 25-35 DEG C or an air energy heat pump drying machine for drying or subjecting the matrix to natural drying under normaltemperature, and crushing to obtain the biocontrol microbial agent. The prepared biocontrol microbial agent is applicable to ralatonia solanacearum antagonism, applicable to salt resistance, drought resistance and soil inorganic phosphorus dissolving, applicable to siderophore and growth hormone heteroauxin synthesis, and applicable to tomato seed germination promotion and growth promotion.

The invention discloses a microbial fertilizer for improving soil, which is prepared from the following raw materials in percentage by weight: Bacillus mucilaginosus 40-45%; potassium fulvate 25-30%;diethyl aminoethyl hexanoate 2-3 %; yeast extract 5-6 %; chitosan oligosaccharide 5-6 %; sodium humate 4-6 %; seaweed extract 4-6 %; compound proteinase 2-3 %; Bacillus licheniformis 4-5 %. The invention can not only improve the soil, but also provide nutrition for the growth and development of plants and enhance the disease resistance of the plants through the mutual cooperation and mutual promotion among the components of the invention. Therefore, the invention can reduce the use amount of chemical fertilizers, improve the soil improvement effect and effectively degrade heavy metals and pesticides in the soil.

The invention relates to lactobacillus agilis and an application thereof and belongs to the technical field of microorganisms. The bacterial strain is a lactic acid bacterium which is separated from a normal microbial flora in intestinal tracts of a healthy pig. By means of Gram staining microscopy, physiological biochemical test and 16s-rRNA sequence analysis, the bacterial strain is identified to be a strain of lactobacillus agilis. Through growth curve drawing, gastrointestinal tract stress resistance environment test and in-vitro enteroadhesive test screening, the lactobacillus agilis strain shows the best biological characters when being compared with other lactic acid bacteria separated from the intestinal tract. The lactobacillus agilis can be applied as a feed additive in production of piglets when being completely cultured. The lactobacillus agilis can promote growth and increase utilization rate of the feed, can reduce diarrhea ratio, improve animal immunity level and improve disease resistance, can effective replace antibiotics in feed additives. The invention provides an excellent bacterial strain for probiotics, and has important significance of improving economic benefit in pig breeding and developing healthy breeding of pigs.

The invention provides a Chinese herbal medicine microbial fermentation preparation and a preparation method thereof, and belongs to the technical field of traditional Chinese medicine ferment.The preparation method comprises the following steps that a Chinese herbal medicine composition is crushed and mixed, water is added to adjust the humidity, then first compound zymophyte is inoculated for fermentation culture, and a solid-state fermentation product is prepared; taking the solid-state fermentation product as a raw material, and preparing a traditional Chinese medicine extracting solution through a solvent extraction method; taking the traditional Chinese medicine extracting solution as a fermentation substrate, and inoculating second compound zymophyte for fermentation culture to prepare the Chinese herbal medicine microbial fermentation preparation. According to the invention, through secondary fermentation, solid fermentation and liquid fermentation are combined, so that effective substances are relatively easy to dissolve out, the growth inhibition of an extracting solution on later fermentation is also reduced, and then the extracting solution is used as a secondary fermentation substrate, so that effective components and active substances of traditional Chinese medicines in the extracting solution are extracted to the maximum extent and are efficiently absorbed and utilized by a human body.

The invention discloses a protein for promoting root surface colorization of trichoderma harzianum and application of the protein, and belongs to the technical field of microorganisms.The HFB small protein family and the function of the HFB small protein family of participating in regulating and controlling of root surface colorization of trichoderma harzianum are found for the first time, and it is shown through research that a remarkable positive regulating and controlling effect is achieved on root surface colorization of trichoderma harzianum through the existence of the family protein.The protein is expressed and secreted in pichia pastoris through the technologies such as yeast heterologous expression and protein fusion.After the protein is purified and enriched, an outer source is added into a water-culture planting system, special protein envelops can be formed on the surfaces of roots, the hydrophilicity / hydrophobicity of the surfaces of roots can be changed, rapid colorization of plant biocontrol growth-promoting bacteria trichoderma harzianum is promoted, and the colorization amount is increased by 110-180% within 48 h compared with that of a control group without the protein.By means of the protein and the application of the protein, the problems that in application of microorganism preparations mainly prepared from trichoderma and related biological fertilizer, the colorization of functional bacteria is poor in effect, and the field application effect is instable can be effectively solved.

The invention discloses a probiotic drop for improving allergic constitution and a preparation method of the probiotic drop, and belongs to the technical field of medical biology. The probiotic drop comprises the following raw materials: lactobacillus casei, lactobacillus paracasei, lactobacillus rhamnosus, lactobacillus reuteri, lactobacillus acidophilus, bifidobacterium longum, lactobacillus plantarum, fermented lactic acid bacteria, galactooligosaccharide, xylooligosaccharide, anhydrous glucose, protein hydrolysate, polyhydric alcohols, natural vitamin E, unsaturated fatty acid, vegetable oil and phospholipid oil. The preparation method of the drop is simple, the process is easy to control, and the drop is stable in performance, not solidified at low temperature, uniform in component, good in oxidation resistance and more stable in storage.

The invention relates to the technical field of microorganisms, in particular to salt-tolerant bacillus and application thereof. The salt-tolerant bacillus strain BAH-1 provided by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the salt-tolerant bacillus strain BAH-1 is CGMCC No. 23503. The halotolerant bacillus BAH-1 is high in colonization capacity and breeding speed in plants, has the effect of efficiently inhibiting plant pathogenic bacteria, has the effect of simultaneously treating plant diseases such as red crown rot, brown spot and stem black spot disease, is high in control effect, has a very good application prospect in plant disease control, and has a wide application prospect. And a foundation is laid for the development of efficient and broad-spectrum biocontrol preparations.

The invention provides a composite microbial bactericide, and a preparation method and application thereof. The composite microbial bactericide is composed of the following components in parts by weight: 10-20 parts of composite microbial inoculum, 10-20 parts of nutritional ingredient, 4-6 parts of enzyme activated substance and 54-78 parts of bactericide carrier, wherein the composite microbial inoculum contains biocontrol growth-promoting bacteria and a protein-decomposing bacterium; the biocontrol growth-promoting bacteria include Trichoderma harzianum, Trichoderma viride and Bacillus licheniformis; and the protein-decomposing bacterium is Bacillus subtilis. The invention also provides a preparation method of the composite microbial bactericide. The composite microbial bactericide provided by the invention can be colonized and grow quickly at the rhizosphere of a plant and form a protecting cover, thus preventing the infection of pathogenic fungi at the root; antibiotics and other plant growth-promoting substances are generated in the pathogenic bacterium interaction and hyperparasitization processes; multiple diseases of crops can be remarkably prevented and antagonized or be directly killed; and meanwhile, the composite microbial bactericide has a root promotion and germination accelerating effect and can promote the healthy growth of the crops, thereby improving the yield and the quality.

The invention discloses a pepper seedling raising method suitable for winter and spring in northern areas, which belongs to the technical field of line pepper planting and comprises the following steps: (1) substrate preparation, (2) seed treatment, (3) germination accelerating treatment and (4) sowing and seedling raising. The method is simple in overall step and easy to popularize and apply, and the cultivated pepper seedlings are stable in quality and high in survival rate.

The present invention discloses a Maggi Mech's yeast T‑2, the storage number of which is: CGMCC No. 18253, and the application of the Maggi Mech's yeast in sterilization and preservation of fruit. Especially the application of the Maggi Metschniella in inhibiting and killing Botrytis cinerea and / or Alternaria. Fermenting the Maggi Mech's yeast to obtain a fermented liquid, the fermented liquid is used for sterilization and preservation of fruit. Fermenting the Maggi Metchi yeast to obtain its metabolites, which are used for sterilization and preservation of fruit; the metabolites are liquid metabolites or gaseous metabolites. The above-mentioned metabolites can also be used as a fungicide for fruit sterilization and preservation. This technology can not only be used as a fruit fungicide, but also has good safety, low temperature resistance, no side effects, simple operation and strong practicability.

The invention relates to the technical field of calf breeding, in particular to a feed for calves and a calf raising method. Buffalo calves are bred by a voluntarily research and developed feed and raising method, quick colonization and propagation of probiotics in intestinal tracts of buffaloes can be accelerated, and the intestinal tract environment of the buffaloes can be improved. According tothe method, the buffaloes are promoted to eat more mainly through improving the mouth feel of the feed and increasing the probiotics; and the feed does not use hormones, so that the appetite and theimmunity of the buffaloes bred with the feed are better than those of the buffaloes raised by a conventional method, and the meat quality is better.

The invention provides a soluble straw decomposing agent, its preparation method and application. The soluble straw decomposing agent comprises by weight: 10-20 parts of a composite bacterial agent, 20-50 parts of nutrients, 2-5 parts of a plant source emulsification penetrant, and 25-68 parts of a soluble carrier. The composite bacterial agent contains cellulose decomposition bacteria, protein decomposition bacteria, and nutrient bio-control growth-promoting bacteria. The invention also provides a preparation method of the above soluble straw decomposing agent. The soluble straw decomposing agent provided in the invention is in a powder form and is fully soluble in water, and also can be applied through both scattering and spraying, thus being suitable for mechanized operation. The soluble straw decomposing agent provided in the invention can substantially speed up degradation of a plurality of crop straw, promote crop growth, and prevent a lot of crop diseases and pests.

The invention provides a biological organic fertilizer with high-cadmium-pollution-resistance bacterial strains. The biological organic fertilizer is composed of, by weight, 4 parts to 6 parts of core microbial agents, 1 part to 3 parts of ion antagonists, and 91 parts to 95 parts of refined organic fertilizers. The invention further provides production and application of the biological organic fertilizer with the high-cadmium-pollution-resistance bacterial strains. The microorganism bacterial strains in the core microbial agents are dominant strains which are specially screened out of sludge of rice field fishponds in cadmium pollution areas. The microorganism bacterial strains are powerful in function and can rapidly grow in soil in a field planting mode to form strong bacterial colonies. The chemical form of cadmium in soil is changed through the live and multiply effect of the microorganism bacterial strains so that the cadmium can be fixed or passivated or detoxified, and the mobility and the bio-availability of cadmium in the soil environment are reduced. The bacterial strains can directly adsorb heavy metal ions Cd+ to microbial cell walls or cells for metabolism, and the cadmium is reduced, purified and fixed through the combined action of the ion antagonists and the refined organic fertilizers.

The invention provides a compound microbial agent for preventing and treating crop scab and a preparation method and a use method thereof. The compound microbial agent is high in pertinence to crop bacterial scab, the mutual synergistic effect of microorganisms is reasonably utilized, the rhizosphere ecological environment of crops is improved through life activities of active microorganisms contained in the microbial agent, and nutrient elements which cannot be directly utilized by crops in the soil are converted into available quick-acting nutrients for the crops. In the aspect of disease treatment, the microbial agent adopts the principle of treating bacteria with bacteria, and the growth of pathogenic bacteria in soil is inhibited by using antibiotic substances secreted by antagonistic strains, so that the pathogenic bacteria cannot reach the pathogenic quantity in the soil, and cannot infect crops. Thecompound microbial agent prevents the crops from attacking diseases, and is strong in adaptability and good in stability. The compound microbial agent can efficiently antagonize potato scab, tomato pepper scab and winter jujube scab, effectively reduce pesticide residue, cannot pollute the environment, and has the effects of fixing nitrogen, dissolving phosphorus and potassium, stimulating plant growth, improving crop quality and the like, so that the purposes of increasing yield and producing high yield are achieved.

The invention relates to a water depth optimization and rapid recovery method of a Scirpus nipponicu wetland under saline-alkali stress. The method includes collecting Scirpus nipponicu corms for seedling raising, sowing the seedlings in different culture bowls, placing the seedlings in a water tank to build a water depth gradient, and adding a Hoagland semi-nutrient solution with different concentrations of NaHCO3+NaCl for culture; and after the culture, measuring the aboveground, underground and corm biomass of the Scirpus nipponicu, and analyzing the optimum water depth for the growth of the Scirpus nipponicu under different saline-alkali concentrations. The results show that when the concentration of saline-alkali is 0, the water depth is adjusted to 0cm; when the saline-alkali concentration is 2.5 g kg-1, the water depth is adjusted to 15-30cm; when the saline-alkali concentration is 5.00 g kg-1, the water depth is adjusted to 15cm; and when the saline-alkali concentration is larger than 10.0 g kg-1, the Scirpus nipponicu cannot survive. By studying the influence of water depth on the growth of the Scirpus nipponicu under different saline-alkali stress degrees, the optimal water depth for the growth of the Scirpus nipponicu under different saline-alkali concentrations is determined, the biomass accumulation of the Scirpus nipponicu is effectively improved, and the rapid colonization and recovery of Scirpus nipponicu population are facilitated.

The invention discloses a mildew Mycoplasma pink, its solid fermentation inoculant and its application, and aims to solve the technical problems of chemical pollution control, complicated biocontrol bacteria screening and complicated production process. Screening a strain of Slime sclerotiorum ( Clonostachys rosea ) NF‑06, whose deposit number is CGMCC No.16262. Culturing and preparing a solid fermentation inoculum obtained by solid fermentation of Mycoplasma pink strain NF-06. The Myxoplasma pink strain NF-06 or the solid fermentative inoculum is used in the control of plant root-knot nematode disease. The method of the invention is simple and feasible, low in cost, high in effective viable bacteria count of the spore powder, friendly to ecological safety, less chemical agent is used, simple in production process, low in cost, and beneficial for application and popularization.

The invention provides a preparation method and application of a biological seedling raising substrate of chaetomium globosum, and belongs to the technical field of agricultural microorganisms. The method comprises the steps that after preliminary fermentation is performed on agricultural waste, the agricultural waste is inoculated with chaetomium globosum ND35 sexual conidial powder to perform secondary fermentation, so that a functional flora taking chaetomium globosum as the main microorganisms in the substrate, and the biological seedling raising substrate of chaetomium globosum is obtained. The agricultural waste comprises, but is not limited to, bark, wood chips, straw and the like. The preparation method and application of the biological seedling raising substrate of chaetomium globosum have the advantages of being simple, being capable of being directly applied to the seedling raising stages of tomatoes, cucumbers and other plants, planting chaetomium globosum ND35 bacterial strains into the plants more directly and quickly, and being beneficial to the plants perpetually; the biological seedling raising substrate of chaetomium globosum is an excellent seedling raising substrate, can be applied to industrialized seedling raising of various plants, fills in the blank of domestic excellent substrates, replaces imported substrates, promotes the healthy development of industrialized seedling raising of vegetables, and has great economic and social benefits.