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Trichoderma viride strain and application thereof

A technology of Trichoderma viride and Trichoderma viride, applied in application, chemicals for biological control, biocides, etc., can solve the problems of long period of disease-resistant varieties, environmental pollution, single resistance, etc., and achieve good application prospects , improve crop yield, the effect of fast growth

Active Publication Date: 2013-02-13
SHANDONG FOREST SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disease-resistant variety has a long cycle and a single resistance
The use of chemicals for chemical control takes a long time and a large amount of use, resulting in pesticide residues and environmental pollution; at the same time, the long-term use of chemical pesticides makes pathogenic bacteria resistant to them, resulting in decreased control efficiency or even failure

Method used

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  • Trichoderma viride strain and application thereof
  • Trichoderma viride strain and application thereof
  • Trichoderma viride strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Isolation and purification of Trichoderma viride (T.viride) WLTR35 (CGMCC No.6673)

[0030] Trichoderma viride (T.viride) WLTR35 (CGMCC No.6673) of the present invention is obtained from mushrooms in Wulian County by using the method of scribing on a plate to separate them. The separation method is as follows:

[0031] ⑴Isolation of Trichoderma strains: Take seriously damaged mushroom bags, first use 75% alcohol to treat them locally for 1 minute, and then treat them with ultraviolet light on the ultra-clean workbench for 20 minutes, cut the treated parts with a sterilized blade, and inoculate A small amount of Trichoderma spores were picked up with a needle, streaked on a PDA plate, and the growth of the colony was observed regularly. Then, the Trichoderma strain was purified by the streaking method on a plate, and transferred to a PDA test tube slant for storage for later use.

[0032] (2) Screening of Trichoderma strains highly effective against cucumber anthracn...

Embodiment 2

[0060] 1. Trichoderma viride fermentation process

[0061] PDB medium formula: 200g potato (peeled), 20g glucose, 1000mL distilled water.

[0062] A large amount of solid fermentation medium formula (mass percentage):

[0063] Solid material: corn cob 60%, bran 40%

[0064] Inorganic salt solution: 3.5% potassium dihydrogen phosphate, 0.04% magnesium sulfate, 4% ammonium sulfate, and the rest is water.

[0065] The solid-to-liquid ratio is 1:1.8 (mass ratio)

[0066] Mass solid fermentation process of Trichoderma viride (T.viride) WLTR35 (CGMCC No.6673):

[0067] ① Strain seed liquid culture: Pick a small amount of spores of Trichoderma viride (T.viride) WLTR35CGMCC No.6673 from the inclined surface of the test tube, transfer them to PDB liquid medium, and culture them on a shaker at 28°C for 3-5 days. These are the seeds liquid.

[0068] ②Cultivation of solid-producing strains: Inoculate the seed liquid into solid medium (500mL Erlenmeyer flask) at a mass ratio of 10%, a...

Embodiment 3

[0071] This example provides related experiments on the control and yield increase of Trichoderma viride (T.viride) WLTR35 (CGMCC No.6673) raw powder on cucumber anthracnose.

[0072] 1. Drugs to be tested

[0073] Trichoderma viride WLTR35 raw powder (prepared in Example 2), 70% mancozeb wettable powder (commercially available).

[0074] 2. Test crops and control objects

[0075] The test crop is cucumber, and the variety is Changchun mici;

[0076] The control object is anthrax.

[0077] 3. The situation of the test site

[0078] The test site is located in the Shouguang cucumber greenhouse planting base in Shandong Province. Cucumber anthracnose has occurred seriously in the greenhouse in recent years, and it is difficult to control. The experiment was carried out in a cucumber greenhouse of 600 square meters (0.9 mu). The soil quality was light loam with an organic matter content of 1.09. %, the pH value was 6.7, the cultivation conditions and management measures of al...

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Abstract

The invention discloses a Trichoderma viride strain and application thereof. The Trichoderma viride stain is WLTR35, and the collection number is CGMCC No.6673. The invention also discloses application of the strain or biological control preparation comprising the strain in controlling plant soil-borne disease. The experiment indicates that the Trichoderma viride strain WLTR35 has the characteristics of fast growth, large spore production quantity, wide action spectrum and the like, can quickly and massively implement colonization, and therefore, has wide application prospects. The biological control preparation prepared from the Trichoderma viride WLTR35 can efficiently control the plant soil-borne disease and effectively enhance the crop yield.

Description

technical field [0001] The invention belongs to the field of biological control of plant diseases. Specifically, the present invention relates to a strain of Trichoderma viride and its application, and a biological control agent prepared by using the Trichoderma viride strain. Background technique [0002] Plant diseases seriously affect the growth of agricultural and forestry crops, and even cause crop death, resulting in significant economic losses. For the prevention and control of plant diseases, conventional methods include traditional chemical control, breeding of disease-resistant varieties, and biological control. The disease-resistant variety has a long cycle and a single resistance. Chemical control uses a long time and a large amount of pesticides, resulting in pesticide residues and environmental pollution. At the same time, the long-term use of chemical pesticides makes pathogenic bacteria resistant to them, resulting in reduced control efficiency or even fail...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
Inventor 牛赡光王清海刘幸红段春华戴宝
Owner SHANDONG FOREST SCI RES INST
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