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Bacillus safensis as well as bio-control preparation and application thereof

A technology of bacillus saphobia and biological control preparations, applied in the directions of biocides, biochemical equipment and methods, and chemicals for biological control, etc., can solve problems such as damage to fruit trees and vegetables, economic losses, etc. , the effect of fast growth and good application prospects

Inactive Publication Date: 2016-11-16
青岛中达农业生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Colletotrichum gloeosporioides Penz is widely distributed and has various hosts. It can infect a variety of plants and cause anthracnose, especially causing serious damage to fruit trees and vegetables, causing huge economic losses.

Method used

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  • Bacillus safensis as well as bio-control preparation and application thereof
  • Bacillus safensis as well as bio-control preparation and application thereof
  • Bacillus safensis as well as bio-control preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0019] 1. Isolation and purification of Bacillus safensis ZDC-02

[0020] (1) Shafu bacillus (Bacillus safensis) ZDC-02 separation method of the present invention

[0021] Bacillus safensis (Bacillus safensis) ZDC-02 of the present invention is separated and obtained by the dilution coating method, and the specific separation method is: take 1g of the bottom mud sample and dissolve it in 99mL of sterile water, place it on a shaker for 5-10min, Mix the sample well with water to obtain 10 -2 Bottom sludge dilution. Take 1ml of the above sediment dilution to 9ml sterile water, shake well to obtain 10 -3 Bottom sludge dilution. Prepare 10 in the same way -4 、10 -5 、10 -6 sediment dilution. take 10 -4 、10 -5 、10 -6 Put 100 μL of the diluted solution of the bottom mud on the poured 2216E medium plate, spread the diluted solution evenly with a spreader, and repeat 3 times for each concentration. The culture dish was cultured in an incubator at 28-30°C for 24 hours, and the...

Embodiment 2

[0031] Example 2 Fermentation process of Bacillus fumigatus strain ZDC-02

[0032] LB liquid medium, the formula is: tryptone 10g, yeast extract 5g, sodium chloride 10g, distilled water 1000mL, pH adjusted to 7.0-7.2.

[0033] 2216E liquid medium, the formula is: yeast extract 1g, peptone 5g, filtered seawater 1000mL, pH adjusted to 7.4-7.5.

[0034] A large amount of fermentation medium is a solid medium, and its formula is solid material and inorganic salt solution, and the ratio of described solid material and inorganic salt is 98.5:0.5 (mass ratio); The composition of described solid material is bran: rice husk: Corn flour: soybean meal = 80:10:5:5 (mass ratio); the inorganic salt includes 20% potassium dihydrogen phosphate, 5% magnesium sulfate, 10% ammonium sulfate, 65% light carbonic acid according to the mass percentage calcium;

[0035] Mass solid fermentation process of Bacillus suffolus strain ZDC-02:

[0036] (1) Activate the Bacillus suffolus strain ZDC-02 stor...

Embodiment 3

[0040] Example 3 Verification of indoor effect of Bacillus shamfu bacillus strain ZDC-02 on pepper anthracnose

[0041] 1. Experimental method

[0042] Adopt flat-plate confrontation culture method, the capsicum anthracnose pathogenic bacteria [Colletotrichmgloeosporioides (Penz.) Penz.et Sacc.] bacterium cake (8mm) that will be tested is moved to the center of the flat plate, ZDC-02 bacterial strain is inoculated at 2cm around, and it is placed in 28- Cultivate in a constant temperature incubator at 30°C, and use a petri dish not inoculated with Bacillus as a control, and each treatment is repeated 3 times. When the control hyphae covered the plate, the diameter of the colony and the diameter of the inhibition zone were measured by the cross method, and the average value and relative inhibition rate were calculated.

[0043] 2. Results

[0044] It can be seen from Table 1 that Bacillus suffolus strain ZDC-02 has a strong inhibitory effect on the mycelial growth of Capsicum ...

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Abstract

The invention discloses a bacillus safensis strain ZDC-02, and belongs to the technical field of biological control of plant diseases, wherein the strain is preserved in China General Microbiological Culture Collection Center with preservation number of CGMCC No.12217. Experiments indicate that the bacillus safensis strain ZDC-02 has the characteristics of being rapid in growth speed, broad in action spectrum, strong in stress resistance, capable of achieving rapid mass colonization on plant bodies and the like; therefore, the strain has a good application prospect. A bio-control preparation, as a bio-control preparation which has a quite broad application prospect and is prepared from the bacillus safensis, not only can effectively prevent and control various plant diseases but also can effectively improve a crop yield.

Description

technical field [0001] The invention relates to the technical field of biological control of plant diseases, in particular to a bacillus shamfu and its biological control preparation and application. Background technique [0002] Colletotrichum gloeosporioides Penz is widely distributed and has various hosts. It can infect a variety of plants and cause anthracnose, especially causing serious damage to fruit trees and vegetables, causing huge economic losses. The use of chemical pesticides has been the key to the control of anthracnose for many years. However, with the increase of strain resistance and the problems of pesticide residues caused by chemical pesticides, it is particularly important to find new control methods for the prevention and control of anthracnose. As a new control method, biological control is safe for humans and animals, does not pollute the environment, and is not easy to produce drug resistance. Therefore, the use of microorganisms to control plant di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/07
CPCA01N63/10C12N1/205C12R2001/07
Inventor 张德超祝帅朱凤蒙
Owner 青岛中达农业生物科技有限公司
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