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Paecliomyces.sp strain for preventing and treating myzus persicae

A Paecilomyces spp., Paecilomyces spp. technology, applied in the directions of microorganism-based methods, applications, fungi, etc.

Inactive Publication Date: 2016-04-13
TOBACCO RES INST CHIN AGRI SCI ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, chemical control is still the most effective way to control Aphids, and there are also many successful applications of biological control methods (such as the reproduction and release of the natural enemy Aphis persicae, etc.), but the use of marine microbial secondary metabolites to explore The development of biological agents for aphids has not been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Field control effect experiment of embodiment 1 bacterial strain mfn21 fermentation supernatant to tobacco aphid

[0033]The strain mfn21 was fermented and cultivated in large quantities. After 8 days, the fermentation liquid was collected, the bacteria were filtered through 8 layers of gauze, and the fermentation supernatant was taken for field control effect experiments. During the peak period of tobacco aphid occurrence in the tobacco field, use a 1L sprayer to evenly spray the fermentation supernatant on the front and back of the leaves with aphids on the upper part of the tobacco plant. Investigate the base number of insect populations before spraying, and investigate the number of remaining live insects on the 1st, 3rd, and 7d after spraying. According to the investigation results, the corrected control effects of the fermentation supernatant on the 1st, 3rd, and 7th days were 83.2%, respectively. 89.6%, 8793%. The results showed that the fermentation supernatant...

Embodiment 2

[0034] Example 2 Bioassay of bacterial strain mfn21 secondary metabolite active substance on aphid

[0035] Carry out a large amount of fermentation culture on the strain mfn21, collect the fermentation broth after 8 days, filter the bacteria through 8 layers of gauze, extract 3 times with an equal amount of ethyl acetate, combine the organic phase for distillation, and transfer the obtained substance to a 5mL centrifuge tube for elution with 5mL acetone When the room temperature is evaporated until the liquid level no longer drops, it is used as a bioassay solution for later use. Select tobacco leaves with 10-15 aphids and place them in a petri dish. Dilute the solution to be tested with acetone into 5 concentration gradients, namely 500 μL / mL, 250 μL / mL, 125 μL / mL, 62.5 μL / mL, and 31.25 μL / mL. Use a 0.0776 μL capillary micro-dropper and use the drop method. Spot measurement, set acetone as a control, repeat 4 times for each treatment, investigate and count the number of dea...

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PUM

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Abstract

The invention aims to provide a strain for preventing and treating myzus persicae. The preservation number of the strain is CGMCCNO. 11659. The mfn21 strain obtained by the invention has a biological activity on the myzus persicae, and can be applied to prevention and treatment of the myzus persicae on tobacco. A bioassay adopting a micro-drop method shows that the lethality of a secondary metabolite in a fermentation broth to the myzus persicae can reach up to 97.78%. After chemical analysis and structural identification by adopting GC / MS is performed, the secondary metabolite comprises seven main compounds including two alkaloid compounds, two ester compounds, a glycoside compound, a peptide compound and an alcohol compound.

Description

technical field [0001] The invention belongs to the technical field of microorganism prevention and control, and in particular relates to a Paecilomyces bacterial strain used for preventing and treating aphids. Background technique [0002] As people pay more and more attention to their own health and living environment, the disadvantages brought by traditional chemical pest control measures have attracted great attention. The excessive use of chemical pesticides has led to the increase of pesticide residues in the environment, the enhancement of pest resistance and the re-emergence of pests and other problems are the most prominent. With the further understanding of various social problems, environmental problems, and human health problems caused by chemical pesticides in various countries around the world, more and more chemical pesticides are restricted or banned. As a new type of pesticide with low toxicity and low residue, and pests and diseases are not easy to produce...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/79
CPCA01N63/30C12N1/145C12R2001/79
Inventor 王秀芳任广伟王凤龙徐蓬军王新伟陈丹
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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