Microbe preparation for curing bacteroidal grancille wilt of plant and its method as well as usage
A microbial preparation and plant technology, applied in the field of microorganisms, can solve the problems of no control effect and achieve the effects of strong biological control, growth promotion, and increased yield
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Embodiment 1
[0029] Example 1 Isolation and screening of bacterial strain HY96-2
[0030] In this example, Ralstonia solanacearum strain (Ralstonia solanacearum) No. 1 physiological race Tb and No. 2 physiological race Tt, vegetable solanum blight, tobacco Alternaria Alternaria, rice blast fungus, cucumber Fusarium wilt and soybean root rot Fusarium bacteria as indicator strains.
[0031] The healthy and diseased tomato plants were collected from different degrees of bacterial wilt in the suburbs of Nanchang, and some of the soil around the root system was sealed in a clean plastic bag and brought back indoors for separation as soon as possible. Divide the collected samples into the following 3 parts: root circle, shake the plant vigorously, and the shaken soil is the rhizosphere part; rhizosphere: the soil that is firmly attached to the root surface after shaking, washed with water, is the rhizosphere part . Root surface: the washed root is cut into small pieces, mixed with quartz sand ...
Embodiment 2
[0043] Identification of embodiment 2 HY96-2 bacterial strain
[0044] The HY96-2 strain isolated from the tomato rhizosphere soil in Nanchang obtained in the above-mentioned Example 1 is further identified below.
[0045] Staining: Gram staining and acid-fast staining were carried out according to conventional methods in this field.
[0046] Morphological characteristics: culture on nutrient agar and beef immersion agar medium at 32°C for 2 days, take a smear of the bacteria, observe the morphology of the bacteria with an optical microscope after staining, and observe the surface characteristics of the cells with an electron microscope.
[0047] Cell wall chemical classification: analysis of amino acids and glycoforms of the whole cell hydrolyzate of the bacteria by thin plate chromatography.
[0048] Culture characteristics: After cultured on LB agar, nutrient agar, glucose yeast extract agar and beef extract agar at 32°C for 2-3 days, observe the colony formation and color...
Embodiment 3
[0060] The fermentation culture of embodiment 3 HY96-2
[0061] 1) 5L automatic fermenter culture
[0062] After the HY96-2 seeds were activated, the strains were cultured. First gelatinize the starch, then add yeast powder, protein powder, glucose, MgSO 4 、KH 2 PO 4 and CaCO 3 medium, sterilized at 121°C for 30 minutes.
[0063] Wash the thalline in the eggplant bottle with sterile water, and inoculate it into a 5L fully automatic fermenter. Under the conditions of an aeration ratio of 0.4-2:1, a rotating speed of 300-800 rpm, and a temperature of 25-35° C., the fermentation time is 24-48 hours. Adopt plate counting method (referring to " plant disease research method ", Fang Zhongda compiles, 1979, Agricultural Press) to record its final bacterial concentration to be 1.37 * 10 12 CFU / ml.
[0064] 2) 50L automatic fermentation tank culture
[0065] After the seeds are activated, the strains are cultivated. The starch is first gelatinized, and then added to the above...
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