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A microbial composition for preventing and treating tobacco bacterial wilt and its application

A microbial composition and grassland technology, applied in the field of microorganisms, can solve the problems such as the difficulty of completely eliminating the pathogenic bacteria of tobacco bacterial wilt and increase the negative impact on the environment, and achieve the effect of improving the microbial flora and the number of effective leaves

Active Publication Date: 2020-01-03
四川省食用菌研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to prevent and control tobacco bacterial wilt and increase tobacco production, a large number of chemicals and fertilizers have been developed and applied, combined with other control and management measures. Although such drugs and fertilizers can temporarily control the disease, it is difficult to completely eliminate the pathogenic bacteria of tobacco bacterial wilt , and chemical inputs can increase the negative impact on the environment

Method used

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  • A microbial composition for preventing and treating tobacco bacterial wilt and its application
  • A microbial composition for preventing and treating tobacco bacterial wilt and its application
  • A microbial composition for preventing and treating tobacco bacterial wilt and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1, the screening of optimal microbial composition obtains

[0037] 1. Screening of antagonistic bacteria

[0038] 1. Medium

[0039] TM medium: peptone 10.0g, glucose 5.0g, casein hydrolyzate (Trypticase) 1.0g, agar powder 18.0g

[0040] Beef extract-peptone medium: 3.0g beef extract, 5.0g peptone, 5.0g NaCl, 20.0g agar, 1000mL distilled water, pH 6.8-7.2

[0041] 2. Determination of antagonistic ability

[0042] Tobacco bacterial wilt pathogen, donated by Yunnan Academy of Tobacco Science.

[0043]Inoculate the pathogenic bacteria in liquid TM medium, shake and culture at 30°C and 170r / min for 36 hours, collect the bacteria by centrifugation and resuspend them with sterile water, place them in a sterile spray bottle sterilized with 70% alcohol and 10% hydrogen peroxide, and then evenly Spray 0.2mL per plate on the TM plate to make a plate containing pathogenic bacteria, and use a 6mm sterile puncher to punch holes in three directions at equal distances f...

Embodiment 2

[0056] Embodiment two. The following is a potted test example

[0057] 1. Processing settings:

[0058] Control (CK): no bacterial solution

[0059] Treatment: Composition (L2-003-A-5+SC-105-B-10+SC-168-A-2+MT-002-B-7) fermented broth, i.e. "1234" described in Example 1 combination.

[0060] Soil: The potting soil is paddy soil sterilized at 121.3°C for 3 hours.

[0061] 2. Culture medium

[0062] TM culture medium: peptone 10.0g, glucose 5.0g, casein hydrolyzate (Trypticase) 1.0g LB culture medium: tryptone 10.0g, yeast extract 5.0g, NaCl 10.0g, pH value 7.4

[0063] 3. Preparation of composition fermentation broth and application method

[0064] Introduce the pathogenic bacteria of tobacco bacterial wilt into TM culture medium, culture it with shaking at 30°C and 170r / min for 24 hours, and adjust its concentration to 10 with sterile water. 9 cfu / mL spare. The inoculation method of R. solanacearum pathogenic bacteria in treatment and CK treatment adopts the stem base p...

example 3

[0125] Example three. The following is a field test example

[0126] 1. Preparation method of microbial composition fermented liquid

[0127] According to Example 1, the 4 strains in "1234" were combined to configure a composite strain. Inoculate one loop of each of the four strains into a 500ml Erlenmeyer flask filled with 250ml LB culture medium, ferment and culture in a shaking table at 30°C and 180r / min for 48h to form a seed solution. The fermenter is used to amplify the cultivation of the seed solution. The fermentation conditions are 36 hours of fermentation time, 0.5 V / V.M of ventilation, 250 r / h of rotation speed, and 30° C. of temperature, and the fermented liquid of the composition is formed.

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Abstract

The invention belongs to the field of microbes, and particularly provides a microbial composition for preventing and treating bacterial wilt of tobacco. The microbial composition is formed by combining three actinomyces bacteria of streptomyces microflavus CGMCC (China General Microbiological Culture Collection Center) 12841, streptomyces albidoflavus CGMCC 12842 and streptomyces pratensis CGMCC 12843 and one pseudomonas aeruginosa. The microbial composition can be used for effectively preventing and treating the bacterial wilt of the tobacco, is used for ameliorating the agronomic traits of the tobacco, adjusting the proportion of a beneficial microbe in soil and improving the yield of the tobacco and the proportion of superior cigarette. In addition, the invention also provides a culture method of the microbial composition.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a microbial composition and its application for preventing and treating bacterial wilt of tobacco. [0002] technical background: [0003] Tobacco is one of the important economic crops in my country. As of 2010, tobacco planting area was 1.35 million hectares, accounting for 0.86% of the total cultivated area. my country has a wide range of tobacco planting areas, and as far as the main tobacco cultivation distribution areas are concerned, the tobacco leaf production areas are concentrated in the economically underdeveloped areas of Yunnan, Guizhou, Sichuan, Chongqing, Hunan and other places, and the economic benefits brought by flue-cured tobacco to farmers are far greater than other crops Therefore, tobacco leaf has become an important economic crop in tobacco growing areas, and tobacco leaf production has become a pillar planting industry in these planting areas and a pillar incom...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00A01P1/00C12R1/465C12R1/385
CPCA01N63/00C12N1/20A01N2300/00
Inventor 吴翔甘炳成彭卫红谢丽源黄忠乾谭昊周洁
Owner 四川省食用菌研究所
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