The invention discloses a composition with efficacies of head infection resisting, itching relieving and alopecia preventing and a preparation method and application of the composition. The composition is prepared from, by weight, 10-500 parts of linseed oil, 10-500 parts of sea buckthorn oil, 10-500 parts of camellia oil, 10-500 parts of cade oil, 10-500 parts of geranium oil, 30-500 parts of menthol, 1-400 parts of radix glycyrrhizae extract, 1-400 parts of red date extract, 1-400 parts of cortex moutan extract, 1-400 parts of psidium guajava extract, 1-400 parts of herba centellae extract, 1-400 parts of rhizoma ligustici wallichii, 1-400 parts of black tea extract, 1-200 parts of phytosterol, 0.1-200 parts of pepper extract, 1-200 parts of flos sophorae immaturus extract, 1-200 parts of ginger extract, 1-200 parts of aloe extract, 1-200 parts of humulus lupulus extract, 1-200 parts of herba ecliptae extract, 1-200 parts of radix angelicae sinensis extract, 1-200 parts of ginkgo extract, 1-200 parts of morinda citrifolia extract, 1-200 parts of oat glucan, 10-300 parts of inositol, 10-300 parts of tocopheryl acetate, 0.1-5 parts of biotin and 1-50 parts of fullerene. The composition has the efficacies of head infection resisting, itching relieving, alopecia preventing, hair strengthening and hair growth promoting.