The invention relates to a formula of a matrine-containing and mite-removing soap. According to the formula, sodium hydroxide, lauric acid, stearic acid, peony seed oil, shea butter and a surfactant are adopted as main materials, and matrine, blueberry anthocyanin, a honeysuckle extract, tea tree essential oil, an aloe extract and pearl powder are adopted as auxiliary materials. The formula specifically comprises, by weight, 3-5 parts of sodium hydroxide, 8-10 parts of lauric acid, 15-20 parts of stearic acid, 2-4 parts of peony seed oil, 2-4 parts of shea butter, 25-30 parts of the surfactant, 1-2 parts of blueberry anthocyanin, 1-2 parts of the honeysuckle extract, 1-2 parts of tea tree essential oil, 1-2 parts of the aloe extract, 1-2 parts of pearl powder, 2.5-3.5 parts of matrine and8-10 parts of purified water. The invention also provides a preparation method of the matrine-containing and mite-removing soap. According to the preparation method, a finished product is prepared byadopting four steps. The matrine-containing and mite-removing soap not only has a good skin decontamination function but also can remove mites, whiten the skin, remove freckles, resist aging and remove wrinkles and bacteria on the surface of the skin of the human body, so that the matrine-containing and mite-removing soap brings effective effects of mite removing, skin caring and face beautifyingto users, and the market competitiveness of products is improved.