The invention belongs to the technical field of daily chemicals, and particularly relates to a method for purifying
alkaloid from oat and application of the
alkaloid to preparation of an anti-
allergy soothing composition. The method for purifying
alkaloid from oat comprises the following steps: (1) crushing oat to obtain oat
powder; (2) mixing oat and water according to a weight ratio of 1: (10-15), putting the mixture into a
microwave reactor, and carrying out
microwave-assisted extraction to obtain a mixture, (3) carrying out enzymolysis to obtain an enzymatic
hydrolysate; (4) adding mixed
bacteria into the enzymatic
hydrolysate, fermenting, and sterilizing to obtain
fermentation liquor; and (5) carrying out
centrifugal separation on the
fermentation liquor, carrying out subcritical extraction on solids obtained by
centrifugal separation, and then recovering the
solvent to obtain an extract containing the oat alkaloid. According to the method, the extraction rate of the oat alkaloid is high, and the synergistic effect of the oat alkaloid and the
sophora flavescens extract is good.