Medical skin repair liquid and application thereof

Abstract

The invention belongs to the technical field of skin repair, and particularly relates to a medical skin repair liquid, which is prepared from the following raw materials in percentage by weight: 1 to 10 percent of caffeic acid grafted carboxymethyl chitosan, 1 to 5 percent of sodium hyaluronate, 1 to 8 percent of nano zinc oxide, 0.1 to 1 percent of ethylparaben, 2 to 10 percent of polydimethylsiloxane and the balance of purified water. According to the invention, caffeic acid is grafted on a carboxymethyl chitosan chain, so that the adhesion performance is improved, and meanwhile, a certain film-forming property is achieved. Secondly, sodium hyaluronate can provide a moisturizing effect, and meanwhile, the sodium hyaluronate and the polydimethylsiloxane can be synergistically assisted to form a film. In addition, nano-zinc oxide and ethylparaben provide antibacterial and anti-inflammatory effects. The traditional Chinese medicine composition can effectively relieve red, swollen and painful skin and relieve inflammation, and is beneficial to skin protection. In the using process, the repairing liquid is easily and uniformly smeared on the skin surface, can isolate external stimulation after automatically forming a film, inhibits growth of bacteria on the skin surface, inhibits inflammation, and helps to recover the skin barrier function, so that the repairing of the skin is effectively promoted.

Description

Technical Field

[0001] The invention relates to the technical field of skin repair, in particular to a medical skin repair liquid which can improve skin dryness, relieve skin redness and swelling, reduce inflammation and inhibit bacterial growth. Background Art

[0002] The skin is the first barrier to maintain human health and undertakes important physiological functions. Multiple stimuli from the external environment, such as ultraviolet radiation, mechanical damage, microbial infection, etc., can lead to dryness, redness, swelling, inflammation and even dysfunction of the skin. Specifically, excessive ultraviolet exposure can cause skin sunburn, aging and even increase the risk of skin cancer. In addition, when the skin suffers trauma such as abrasions or cuts, the release of cytokines triggers an inflammatory response, which not only delays the healing process of the wound, but also increases the risk of infection. In order to cope with these skin problems, a variety of skin repair products have appeared on the market. These products are developed and applied according to different needs such as moisturizing, anti-inflammatory and antibacterial.

[0003] In moisturizing products, natural moisturizing factors such as hyaluronic acid, alginic acid and chitosan are widely used in formulas. By supplementing these high-efficiency moisturizing factors, it not only helps to maintain the moisture balance of the skin, but also effectively reduces the sensitivity of the skin, thereby maintaining the softness and elasticity of the skin. However, in the face of the growing demand for diversified skin repair, a single moisturizing function is no longer sufficient to meet the comprehensive needs. In order to improve the application of skin repair products, the existing technology achieves multifunctional integration by scientifically optimizing the formula. For example, adding plant extracts that can relieve inflammation to moisturizing products can further enhance the repair effect of the product. Consumers usually tend to choose repair products containing natural ingredients and with specific functions. Although natural ingredients are favored for their mildness, low irritation and rich nutrition, they are also easy to become a hotbed for microbial growth. Therefore, it is particularly necessary to add preservatives in appropriate amounts. Preservatives can inhibit the growth of microorganisms, prevent the spoilage of products and extend the service life, but they may also cause skin irritation and allergies. In recent years, metal ions have been widely used as preservatives due to their broad-spectrum antibacterial properties and safety. At the same time, they can also effectively replace antibiotics to achieve antibacterial effects, thereby reducing the potential threat to public health posed by excessive use of antibiotics. However, the antibacterial effect of metal ions is also affected by their type, size, etc. Therefore, the selection of metal ions is also particularly important. Existing technologies have developed a variety of product forms including band-aids, gauze dressings, gel dressings, etc. However, the preparation process of these products is relatively cumbersome and not convenient to use. Skin repair liquid is a simple-to-use external product, but it cannot be stably attached to the skin surface for a long time. Therefore, when developing skin repair products, it is necessary to ensure that the product achieves the best balance between practicality, effectiveness and safety through scientific formula design and ingredient optimization.

[0004] In summary, it is crucial to design an efficient skin repair product that can comprehensively improve skin conditions, especially for problems such as dryness, redness, inflammation and bacterial infection, to achieve safe, effective and sustainable skin repair effects. While ensuring that the preparation process is simple and easy to use, such products scientifically integrate multiple functions such as moisturizing, anti-inflammatory and antibacterial, and balance the use of preservatives and antibiotics to ensure that the product can not only significantly improve skin conditions, but also minimize potential health risks, and achieve the goal of safe, effective and sustainable skin repair. Summary of the invention

[0005] Aiming at the diversified demands for skin repair, the present invention provides a medical skin repair liquid composition and a preparation method thereof.

[0006] The composition and its mass percentage are 1%-10% of caffeic acid grafted carboxymethyl chitosan; 1%-5% of sodium hyaluronate; 1%-8% of nano zinc oxide; 0.1%-1% of ethylparaben; 2%-10% of polydimethylsiloxane; and the remaining components are purified water.

[0007] Preferably, the carboxymethyl chitosan is O-carboxymethyl chitosan, which is prepared by introducing carboxymethyl groups into the hydroxyl groups of chitosan for substitution.

[0008] Preferably, the nano zinc oxide has excellent solubility and an average particle size of about 80-100 nm.

[0009] Preferably, the polydimethylsiloxane is hexamethyldisiloxane, which has good auxiliary film-forming property and flexibility.

[0010] Preferably, the specific amounts are 4% caffeic acid grafted carboxymethyl chitosan, 2% sodium hyaluronate, 2% nano zinc oxide, 0.5% ethyl hydroxybenzoate, and 5% polydimethylsiloxane.

[0011] A method for preparing the medical skin repairing liquid as described above specifically comprises the following steps: Step 1: Preparation of caffeic acid grafted carboxymethyl chitosan. First, chitosan and benzaldehyde are added to phosphate buffer, the pH of the mixed solution is adjusted to alkaline, and the mixture is stirred and mixed continuously for reaction. Subsequently, isopropanol and chloroacetic acid are added to continue stirring the reaction. After the reaction is completed, the pH value of the reaction system is adjusted to acidic, the mixture is centrifuged, and repeatedly washed with anhydrous ethanol to finally obtain O-carboxymethyl chitosan. On this basis, the carbodiimide chemical cross-linking method is further used to graft it with caffeic acid. Weigh carboxymethyl chitosan and dissolve it in phosphate buffer, add 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS), and adjust the pH value of the reaction system to 5.0. Finally, caffeic acid is added and the reaction is stirred continuously at room temperature in the dark. After the reaction is completed, it is dialyzed and freeze-dried to finally obtain caffeic acid grafted carboxymethyl chitosan.

[0012] Step 2: Preparation of nano zinc oxide. First, potassium hydroxide and zinc acetate are added to anhydrous ethanol and completely dissolved. Under ice bath conditions, potassium hydroxide solution is added dropwise to the zinc acetate solution, and the reaction is stirred for 1 hour. Subsequently, 3-aminopropyltriethoxysilane is added to the reaction system, and the reaction is continued by stirring. After the reaction is completed, the mixture is centrifuged, repeatedly washed with anhydrous ethanol, and finally freeze-dried to obtain nano zinc oxide powder.

[0013] Step 3: First, weigh caffeic acid grafted carboxymethyl chitosan and dissolve it in purified water, then add nano zinc oxide, sodium hyaluronate, and ethylparaben in sequence and stir to dissolve. Add polydimethylsiloxane in a water bath at 80°C and continue stirring until the mixture is uniform.

[0014] Step 4: After the mixed solution is cooled to room temperature, it is filtered using a 0.22 μm filter to remove any undissolved particles. The filtered solution is then filled into a glass bottle to finally prepare a medical skin repair solution.

[0015] The method of using the above-mentioned skin repair liquid is as follows: First, clean the affected skin. According to the affected area, take an appropriate amount of the skin repair liquid of the present invention and apply it gently and evenly on the skin. Use it 2-3 times a day. Avoid contact with eyes and mouth during use. If allergic reaction or irritation occurs, stop using it immediately.

[0016] The composition of the present invention is described as follows: Caffeic acid grafted carboxymethyl chitosan is a copolymer in which caffeic acid groups are introduced into the carboxymethyl chitosan structure by chemical methods, thereby giving it unique properties. Carboxymethyl chitosan itself, as a natural polymer derivative, has good solubility and biocompatibility. Further studies have shown that carboxymethyl chitosan not only has good moisturizing and antibacterial and anti-inflammatory properties, but also has high viscosity and can form a stable film. In addition, the carboxyl and amino groups contained in carboxymethyl chitosan are conducive to chemical modification. By introducing the polyphenol group of caffeic acid, it can adhere tightly to the surface of the skin and form a protective barrier. Caffeic acid grafted carboxymethyl chitosan has multiple functions such as moisturizing, antibacterial, anti-inflammatory, and film-forming, and can solve diverse needs in practical applications. In the skin repair liquid, caffeic acid grafted carboxymethyl chitosan, on the basis of providing a moisturizing effect, uses its high viscosity and film-forming properties to effectively solve the problem that the traditional repair liquid is too fluid and difficult to act on the skin surface for a long time, while exerting certain antibacterial and anti-inflammatory effects, further providing additional protection for skin repair.

[0017] Sodium hyaluronate is a natural polysaccharide with good moisturizing and biocompatibility, and is widely used in skin repair products. Sodium hyaluronate can absorb and lock moisture on the skin surface, provide long-lasting moisturizing, and improve the skin microenvironment and provide nutrition to the skin.

[0018] Nano zinc oxide is a nano-scale metal oxide with high dispersibility and high specific surface area. It has been widely used in the field of skin repair due to its excellent antibacterial and anti-inflammatory properties and ability to accelerate wound healing. Compared with metals such as silver and copper, zinc oxide is less toxic to the human body. After grain refinement, the particle size of zinc oxide reaches the nanometer level, which not only improves its solubility but also enhances its stability. In the skin repair liquid, nano zinc oxide has little irritation to the skin and exhibits excellent antibacterial properties. It not only effectively inhibits the growth of bacteria on the surface and deep layers of the skin, but can also be used as a preservative to inhibit the growth of microorganisms in the product.

[0019] Ethyl paraben is a paraben preservative with broad-spectrum antibacterial activity. In the skin repair liquid, ethyl paraben is added in a safe and acceptable amount, and through the synergistic effect with nano zinc oxide, it not only enhances the antiseptic effect of the product, but also ensures the safety of use, and at the same time demonstrates its potential in antibacterial applications.

[0020] Polydimethylsiloxane is a chemically stable and physiologically inert organosilicon compound. In skin repair applications, polydimethylsiloxane is less irritating to the skin and can form a barrier on the skin surface to effectively prevent water evaporation and isolate it from external damage. In the skin repair liquid, polydimethylsiloxane not only makes the product easier to apply, but also helps form a uniform film and is not easy to react with other components, thus ensuring the stability of the product.

[0021] Compared with the prior art, the present invention has the following beneficial effects: The invention provides a multifunctional medical skin repair liquid that integrates moisturizing, antibacterial and anti-inflammatory properties. The composition is a mixture of caffeic acid grafted carboxymethyl chitosan, sodium hyaluronate, nano zinc oxide, ethyl hydroxybenzoate and polydimethylsiloxane. The preparation process of the skin repair liquid is simple and controllable, and the functions of each component are clear. Caffeic acid grafted carboxymethyl chitosan, as the main component, not only gives the product excellent moisturizing performance, but also has multiple functions such as antibacterial and anti-inflammatory, providing comprehensive protection for the skin, while sodium hyaluronate is used to further increase the moisturizing performance. The synergistic effect of ethyl hydroxybenzoate and nano zinc oxide not only enhances the antiseptic effect of the product, but also shows excellent antibacterial properties. Finally, with the cooperation of polydimethylsiloxane, it shows the characteristics of easy application and accelerated film formation.

[0022] This medical skin repair liquid has the following characteristics: 1. The ingredients are safe and harmless, with low irritation and non-allergenic properties; 2. It is easy to apply on the skin surface, has good adhesion, and can form a film within minutes; 3. Excellent moisturizing properties maintain the softness and elasticity of the skin; 4. Significant antibacterial and anti-inflammatory effects can effectively relieve skin redness and swelling, reduce inflammation and inhibit bacterial growth. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 The water content growth rate of each group of repair liquid at different times Figure 2 Antibacterial effect of each group of repair solutions on Escherichia coli Figure 3 Antibacterial effect of each group of repair solutions on Staphylococcus aureus Figure 4 Comparison of the antibacterial effect of medical skin repair liquid applied on the skin DETAILED DESCRIPTION

[0024] The present invention is further described in conjunction with specific examples. Except for caffeic acid grafted chitosan and nano zinc oxide, the raw materials used in the following preparations can all be purchased from the market. Example

[0025] A medical skin repairing liquid is prepared, the composition of which is 4% caffeic acid grafted carboxymethyl chitosan, 2% sodium hyaluronate, 2% nano zinc oxide, 0.5% ethyl hydroxybenzoate, 5% polydimethylsiloxane, and the rest is purified water. Example

[0026] On the basis of Example 1, the proportion of caffeic acid grafted carboxymethyl chitosan was increased to 8% to enhance adhesion and film-forming effect. Example

[0027] On the basis of Example 1, the proportion of sodium hyaluronate was increased to 5% to enhance the moisturizing effect.

[0028] Table 1 Specific formula of medical skin repair liquid composition Comparative Example 1 A medical skin repairing liquid is prepared, the composition of which is 4% carboxymethyl chitosan, 2% sodium hyaluronate, 2% nano zinc oxide, 0.5% ethyl hydroxybenzoate, 5% polydimethylsiloxane, and the rest is purified water.

[0029] Comparative Example 2 A medical skin repairing liquid is prepared, the composition of which is 4% caffeic acid grafted carboxymethyl chitosan, 2% sodium hyaluronate, 2% commercially available common zinc oxide, 0.5% ethyl hydroxybenzoate, 5% polydimethylsiloxane, and the rest is purified water.

[0030] In order to prove the beneficial effects of the present invention, performance verification was carried out, and the specific experimental conditions are as follows: Film forming test Take 1 mL of the skin repair solution and apply it evenly on a glass slide, place it in a constant temperature box at 37±1℃, and record the film-forming time.

[0031] Experimental results: The test results are shown in Table 2. The skin repair liquid with caffeic acid grafted carboxymethyl chitosan can form a uniform film within 3 minutes after application.

[0032] Table 2 Film formation time of each group of repair liquid Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Film forming time (min) 2 2 2 5 3 Moisturizing performance test 10 subjects aged between 20 and 35 were randomly selected to test the moisturizing performance in a laboratory environment with a constant temperature of 23°C and a relative humidity of 50%. After cleaning the inner skin of the forearms of both hands, 5 2cm×2cm areas were selected for marking. Before the test, the moisture content of the above areas was tested using a skin moisture meter. Subsequently, Examples 1-3 and Comparative Example 1 were selected for the smearing test. The moisture content was tested using a skin moisture meter 1 hour and 5 hours after smearing, respectively, and the average moisture content growth rate of each group after each test was calculated to analyze the changes in skin moisture content.

[0033] Experimental results: Figure 1 As shown, during the test of the subjects, the repair liquids of each group showed good smoothness when applied on the skin, and no discomfort such as redness, swelling, and stinging occurred, and the skin did not feel tight after film formation. From the test results in Table 3, it can be seen that the skin water content of Examples 1-3 is higher than that of Comparative Example 1. The higher the content of caffeic acid-grafted carboxymethyl chitosan and sodium hyaluronate, the stronger the skin moisturizing ability. It can be seen that the natural moisturizing factor is beneficial to improving the moisturizing performance of the repair liquid.

[0034] Adhesion performance test 2. 10 subjects in the moisturizing performance test were selected. Before the test, they sat quietly in a laboratory environment with a constant temperature of 23℃ and a relative humidity of 50% for 30 minutes. Then, they cleaned their hands and felt the stickiness of each group of repair liquids with their fingertips and palms, and scored them at the same time. The scoring system uses a 10-point system. The higher the score, the stronger the stickiness. The average score of each group was calculated to analyze the adhesion performance of the repair liquid.

[0035] Experimental results: It can be seen from the test results in Table 4 that compared with Comparative Example 1, the stickiness of the rest of the repair solutions is at a higher level. This is because the catechol group of caffeic acid can form hydrogen bonds, electrostatic interactions, etc. with the skin surface. At the same time, the higher the content of caffeic acid grafted carboxymethyl chitosan, the stronger the adhesion performance. Combined with the results of tests 1-3, it can be seen that the repair solution has certain adhesion properties, can quickly adhere to the skin surface, and effectively form a film to form a protective barrier.

[0036] Table 3 Viscosity score results of each group of repair liquid Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Stickiness score 8.0 8.8 8.2 5.5 7.8 Antibacterial performance test 4.1 The skin repairing liquids of Examples 1 and 2 and Comparative Examples 1 and 2 were co-incubated with Staphylococcus aureus and Escherichia coli, respectively. After 1 hour of incubation, the bacteria were counted and the antibacterial rate was calculated to analyze the antibacterial effect of the skin repairing liquid.

[0037] Experimental results: Figure 2-3 As shown, each group of skin repair liquids showed the ability to inhibit bacterial growth, because zinc oxide can destroy the structure of bacteria, thereby effectively inhibiting their reproduction and growth. Compared with Comparative Example 1, the antibacterial effect of the skin repair liquids in each group of examples is more significant, because the carboxymethyl chitosan modified with caffeic acid can further destroy the surface morphology of bacteria, and as the content of caffeic acid grafted carboxymethyl chitosan increases, its antibacterial effect also shows an increasing trend. Compared with Comparative Example 2, the antibacterial effect of Example 2 is also better, mainly because the nano zinc oxide with a smaller particle size can more easily penetrate the bacterial structure and interfere with the synthesis of bacterial nucleic acids.

[0038] 4.2 Two areas on the inner side of the front wall of the subject's hand were selected, one of which was not treated, and Example 1 was evenly applied to the skin of the other. After 1 hour of application, the LB agar plate was applied with a sterile cotton swab. The skin was compared with the skin without the repair solution to observe the antibacterial effect of the skin repair solution in actual application.

[0039] Experimental results: Figure 4 As shown, the use of skin repair solution can effectively inhibit the reproduction and growth of bacteria on the skin surface.

[0040] In summary, the preparation process of the medical skin repairing liquid of the present invention is simple and feasible, its ingredients are safe and harmless, and it is extremely convenient to use, and is not limited by the shape and size of the damaged skin area. The repairing liquid is easy to be evenly applied on the skin surface, and exhibits good adhesion, and can form a film on the skin surface. This film can not only prevent the loss of moisture on the skin surface, but also isolate the stimulation of the external environment. Compared with traditional carboxymethyl chitosan and zinc oxide, the composition after optimized design has a more excellent skin repair effect. Its excellent moisture retention can maintain the softness and elasticity of the skin. At the same time, the significant antibacterial and anti-inflammatory effects can effectively relieve skin redness, reduce inflammation and inhibit bacterial growth, providing all-round and deep repair for the skin.

Claims

1. A preparation method of a medical skin repairing liquid, characterized in that: It is composed of caffeic acid grafted carboxymethyl chitosan, sodium hyaluronate, nano zinc oxide, ethylparaben, polydimethylsiloxane and purified water. in, Caffeic acid grafted carboxymethyl chitosan mass percentage 1%-10%; The mass percentage of sodium hyaluronate is 1%-5%; The mass percentage of nano zinc oxide is 1%-8%; The mass percentage of ethyl paraben is 0.1%-1%; The mass percentage of polydimethylsiloxane is 2%-10%; The remainder is purified water.

2. The medical skin repairing solution according to claim 1, characterized in that: The composition comprises the following components by weight: 4% caffeic acid grafted carboxymethyl chitosan, 2% sodium hyaluronate, 2% nano zinc oxide, 0.5% ethyl hydroxybenzoate, 5% polydimethylsiloxane, and the remaining component is purified water.

3. The medical skin repairing solution according to any one of claims 1-2, characterized in that: The caffeic acid grafted carboxymethyl chitosan is a main component and has high viscosity and film-forming properties, and can exert multiple functions such as moisturizing, antibacterial, and anti-inflammatory.

4. The medical skin repairing solution according to any one of claims 1-2, characterized in that: The polydimethylsiloxane is hexamethyldioxysilane.

5. The method for preparing the medical skin repairing liquid according to any one of claims 1 to 2 comprises the following steps: caffeic acid grafted carboxymethyl chitosan, sodium hyaluronate, nano zinc oxide, and ethylparaben are mixed in proportion, and then a proportion of polydimethylsiloxane is added to the mixture and stirred evenly. Finally, the mixture is filtered and filled to obtain the medical skin repairing liquid.

6. Use of the medical skin repairing solution according to any one of claims 1, 2 or 5 in the field of skin repair.

7. The use according to claim 6, characterized in that: Skin lesions include dry redness, inflammation, and bacterial infection.

8. The use according to claim 6, characterized in that: The medical skin repairing liquid promotes skin repair through multiple functions such as moisturizing, antibacterial, and anti-inflammatory.

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