A scalp care solution containing probiotic fermentation products and preparation method thereof

Abstract

The invention discloses a scalp care solution containing a probiotic fermentation product and a preparation method thereof, which comprises the following raw materials by weight: 87-91 parts of water, 3-5 parts of glycerol, 4-9.3 parts of probiotic fermentation products, 1.3-2.6 parts of plant extracts, 0.4-0.8 parts of 1,2-hexanediol, 0.4-0.8 parts of p-hydroxyacetophenone, 0.1-0.3 parts of carbomer, 0.1-0.2 parts of arginine, 0.1-0.15 parts of trace components, and 7-11 parts of composite nano-carrier liquid. The scalp care solution provides nutrition for scalp probiotics by adding prebiotic fermentation products, such as lactobacillus fermentation products, bifid yeast fermentation product filtrate, altermonas fermentation product extract, and bifid yeast fermentation product lysate, which is beneficial to promoting the growth of scalp probiotics and enhancing the scalp barrier, thereby achieving scalp health.

Description

Technical Field

[0001] The invention belongs to the technical field of daily chemical products, and particularly relates to a scalp care solution containing a probiotic fermentation product and a preparation method thereof. Background Art

[0002] The scalp is the "soil" on which hair depends for survival. A healthy scalp can grow and cultivate beautiful hair. The scalp is similar to other human skin structures, but there are also differences, such as the well-developed sebaceous sweat glands and vigorous sebum secretion of the scalp. If scalp problems are not prevented and controlled in time, the scalp barrier function can be easily damaged, eventually leading to scalp inflammation, hair follicle inflammation, etc.

[0003] In people's consciousness and behavior, they often only pay attention to hair care, thus neglecting scalp care; however, they do not know that bad work and rest habits, external pressure, air pollution, surfactants and other chemical raw materials will cause scalp problems such as itching, dandruff, oiliness, odor, erythema, hair loss, aging, etc. The health of the scalp directly affects the state of the hair. The stratum corneum of the scalp is not only thinner than that of the face, but also has more sebaceous glands, so the requirements for scalp care are higher. Scalp care products need to have the functions of gentle cleansing, effective anti-hair loss, effective dandruff removal and anti-aging for the scalp, to protect the scalp microecological environment, repair the scalp barrier function, enhance the scalp resistance, etc. to maintain the normal metabolic function of the scalp and promote healthy hair growth.

[0004] The scalp microecology is a whole composed of host cells and secretions on the surface of the scalp, scalp microorganisms and the microenvironment in which they are located. Since the scalp has developed sweat glands and sebaceous glands, which can secrete water and lipids and provide nutrients for the growth of microorganisms, there are more microorganisms on the scalp. Under normal conditions, these resident bacteria can seize nutrients and cell binding sites on the scalp, prevent pathogenic bacteria in the external environment from invading the scalp, and maintain the balance of the scalp microecology. A healthy scalp ecological environment is maintained by three major balances: oil, flora, and metabolism; when oil secretion is unbalanced, the scalp will become oily; when the flora environment is unbalanced, harmful bacteria will breed in large numbers and it is easy to itch; when the scalp stratum corneum metabolizes too quickly and falls off, dandruff is formed. Oily scalp, itchy scalp, dandruff, dry hair, hair loss, etc. are all scalp problems caused by an imbalance in the scalp ecological environment. The scalp microbiome is usually composed of bacteria and fungi, generally including Staphylococcus, Propionibacterium and Malassezia. Most staphylococci are non-pathogenic bacteria, and Staphylococcus epidermidis is a resident bacterium that helps the skin to clean itself. The large-scale parasitism and reproduction of Malassezia can induce or aggravate seborrheic dermatitis, hair loss, pityriasis capitis, folliculitis, pruritus, tinea capitis and other diseases.

[0005] Hair is keratin that grows from hair follicles, while the scalp is the skin on the head. Scalp care and hair care cannot be generalized. Scalp care should try to maintain the ecological balance of the scalp and ensure that the steady-state oil, pH value, and moisture content of the scalp microbial flora are within the normal range. Chinese patent CN113142554A discloses a probiotic fermentation liquid for improving dandruff production and its application, comprising the following raw materials in parts by weight: 40-70 parts of Lactobacillus acidophilus, Lactobacillus reuteri, Lactobacillus casei, Lactobacillus rhamnosus, Lactobacillus helveticus, Lactobacillus plantarum, Leuconostoc mesenteroides, Bifidobacterium breve, etc., 9-12 parts of molasses, 10-13 parts of tea solution, 7-10 parts of glucose, 0.8-1.1 parts of sodium tripolyphosphate, 2-5 parts of Polygonum multiflorum, 1-4 parts of Ginkgo biloba leaves, 9-12 parts of Cnidium monnieri, 15-18 parts of Platycladus orientalis leaves, 1-4 parts of Eclipta prostrata, 4-7 parts of defatted soy flour, 5-8 parts of glycyrrhizic acid, 0.7-1 parts of vitamin B6, 1-4 parts of pig liver, 5-8 parts of whey protein, 4-7 parts of cereal powder, 0.5-0.8 parts of emulsifier, 0.2-0.5 parts of stabilizer, and 10-13 parts of purified water. The invention has simple preparation steps and low cost. After application, it can enhance hair quality, inhibit the growth of dandruff, increase human immunity, and has a strong fermented flavor, which is easily accepted by the public. However, according to its specification, the fermented liquid only has a good effect in inhibiting Marsala fungi. Whether it is effective in adjusting the flora environment of the scalp, promoting scalp repair, and enhancing the scalp barrier remains to be discussed. Therefore, the field urgently needs to develop a scalp care liquid, so as to fundamentally achieve scalp health. Summary of the invention

[0006] In view of the deficiencies in the prior art, the object of the present invention is to provide a scalp care solution containing probiotic fermentation products and a preparation method thereof. The scalp care solution provides nutrition for scalp probiotics by adding prebiotic fermentation products, such as lactobacillus fermentation products, bifid yeast fermentation product filtrate, alternator mononas fermentation product extract, and bifid yeast fermentation product lysate, which is beneficial to promote the growth of scalp probiotics, enhance the scalp barrier, and thus achieve scalp health.

[0007] To achieve the above object, the present invention provides the following technical solutions:

[0008] A scalp care solution containing probiotic fermentation products, comprising the following raw materials in parts by weight:

[0009] 87-91 parts of water, 3-5 parts of glycerol, 4-9.3 parts of probiotic fermentation products, 1.3-2.6 parts of plant extracts, 0.4-0.8 parts of 1,2-hexanediol, 0.4-0.8 parts of p-hydroxyacetophenone, 0.1-0.3 parts of carbomer, 0.1-0.2 parts of arginine, 0.1-0.15 parts of trace ingredients, and 7-11 parts of composite nano-carrier liquid.

[0010] Preferably, a scalp care solution containing a probiotic fermentation product comprises the following raw materials, by weight: 88-90 parts of water, 3-4 parts of glycerol, 5-7 parts of probiotic fermentation products, 1.3-2.6 parts of plant extracts, 0.4-0.6 parts of 1,2-hexanediol, 0.4-0.6 parts of p-hydroxyacetophenone, 0.1-0.2 parts of carbomer, 0.15-0.2 parts of arginine, 0.12-0.15 parts of trace ingredients, and 8-10 parts of composite nanocarrier liquid.

[0011] Preferably, a scalp care solution containing a probiotic fermentation product comprises the following raw materials, by weight: 89.63 parts of water, 3 parts of glycerol, 5.5 parts of a probiotic fermentation product, 2.1 parts of a plant extract, 0.55 parts of 1,2-hexanediol, 0.55 parts of p-hydroxyacetophenone, 0.2 parts of carbomer, 0.15 parts of arginine, 0.14 parts of trace ingredients, and 9 parts of a composite nanocarrier liquid.

[0012] Preferably, the probiotic fermentation product is composed of the following raw materials, by weight: 2-4 parts of lactobacillus fermentation product, 1-3 parts of bifid yeast fermentation product filtrate, 0.9-2 parts of alternator mononas fermentation product extract, and 0.1-0.3 parts of bifid yeast fermentation product lysate; the plant extract is composed of the following raw materials: 0.5-1 parts of matsutake extract, 0.3-0.7 parts of Astragalus membranaceus root extract, and 0.5-0.9 parts of melilotus extract.

[0013] In the present invention, the lactobacillus fermentation products mainly include organic acids, bacteriocins, aromatic substances such as acetaldehyde, extracellular polysaccharides, γ-aminobutyric acid, etc. Among them, the organic acids mainly include lactic acid, acetic acid, and a small amount of formic acid, propionic acid, etc., which have antibacterial and preservative effects.

[0014] In the present invention, the filtrate of the bifid yeast fermentation product can promote the growth and regeneration of skin cells. It contains some antibacterial and anti-inflammatory substances, which can effectively inhibit the activity of pathogens. At the same time, it contains rich antioxidant substances, has strong antioxidant capacity, and has the functions of beauty, anti-wrinkle, whitening, moisturizing and the like.

[0015] In the present invention, the lysate of the bifid yeast fermentation product has strong anti-immunosuppressive activity and can promote DNA repair, can effectively protect the skin from damage caused by ultraviolet rays, help prevent photoaging of the epidermis and dermis of the scalp, has the function of strengthening the metabolism of the stratum corneum, can also capture free radicals, inhibit lipid peroxidation, and has whitening and anti-aging functions. In addition, the lysate of the bifid yeast fermentation product also contains nutrients such as proteins, polypeptides and polysaccharides that are beneficial to the skin, which can nourish and moisturize, brighten and resist aging, and resist photoaging.

[0016] In the present invention, the extract of the fermentation product of Alteromonas has antibacterial, anti-inflammatory, antioxidant and other biological activities, which helps to delay scalp aging, and can be dispersed on the stratum corneum on the surface of the scalp to prevent scalp dryness and has good moisturizing properties.

[0017] More preferably, the probiotic fermentation product is composed of the following raw materials, by weight: 2-3 parts of lactobacillus fermentation product, 1-2 parts of bifid yeast fermentation product filtrate, 0.9-1.5 parts of alternator mononas fermentation product extract, and 0.1-0.2 parts of bifid yeast fermentation product lysate; the plant extract is composed of the following raw materials: 0.8-1 parts of matsutake extract, 0.4-0.6 parts of astragalus membranaceus root extract, and 0.6-0.8 parts of melilotus extract.

[0018] In the present invention, the Matsutake extract contains substances such as polysaccharides, proteins, amino acids, polypeptides, steroids, terpenes, polyphenols and vitamins. Among them, polyphenols have good antioxidant activity due to their high reactivity of hydroxyl substitution and free radical phagocytosis ability, and have a large scavenging effect on both hydroxyl and superoxide anion free radicals. At the same time, they also have anti-inflammatory and antibacterial activity and can inhibit the growth of pathogenic microorganisms.

[0019] In the present invention, the Astragalus membranaceus root extract contains flavonoid compounds, such as isoflavones, flavonoid glycosides, etc., and has the effects of anti-oxidation, anti-inflammatory and immunomodulation.

[0020] In the present invention, the Melilotus officinalis extract mainly contains triterpenoid compounds, flavonoid compounds, coumarin compounds, and also contains phenolic acid compounds and sterol compounds, and has anti-inflammatory and antioxidant effects.

[0021] Preferably, the trace ingredients are composed of the following raw materials in parts by weight: 9-10 parts of 1,2-pentanediol, 5-6 parts of disodium EDTA, 0.1-0.3 parts of ethylhexylglycerol, 0.01-0.04 parts of ergothioneine, 0.01-0.03 parts of acetyl octapeptide-3, and 0.01-0.02 parts of arginine / lysine polypeptide.

[0022] More preferably, the trace ingredients are composed of the following raw materials in parts by weight: 9-9.5 parts of 1,2-pentanediol, 5-5.5 parts of disodium EDTA, 0.1-0.2 parts of ethylhexylglycerol, 0.01-0.03 parts of ergothioneine, 0.01-0.02 parts of acetyl octapeptide-3, and 0.01-0.015 parts of arginine / lysine polypeptide.

[0023] Preferably, the preparation method of the composite nano-carrier liquid is as follows:

[0024] S1. Add succinic acid into deionized water, stir to dissolve, then add 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride solution, stir to activate, then add hydroxypropyl chitosan, hydroxypropyl-β-cyclodextrin, and N-hydroxysuccinimide, and react at a constant temperature. After the reaction is completed, add 4 times the volume of dichloromethane for precipitation, filter, wash with ethanol, and dry to obtain a solid product;

[0025] S2, adding the solid product in step S1, whey protein, and Tween 80 into deionized water, stirring evenly to obtain an aqueous phase; adding astaxanthin, artemisia annua oil, and glyceryl distearate into anhydrous ethanol, stirring at 75° C. to obtain an oil phase;

[0026] S3, adding the oil phase in step S2 to the water phase, homogenizing with a high-speed shear emulsifier, dispersing to form an emulsion solution, maintaining a water bath temperature of 75-80°C, continuously stirring at high speed, evaporating the ethanol, and adding 50mL of deionized water at the same temperature to the emulsion, then treating the emulsion with an ultrasonic power of 400W, the ultrasonic frequency is: ultrasonic 5s, interval 5s, continuous ultrasonic 5-8min, after the end of the ultrasonic, the emulsion is placed in an ice water bath to cool, and impurities are filtered through a 0.8μm filter membrane to obtain.

[0027] In the present invention, astaxanthin has a strong antioxidant capacity, can remove nitrogen dioxide, sulfide, disulfide, etc., can also reduce lipid peroxidation, and effectively inhibit lipid peroxidation caused by free radicals. However, due to its characteristic of being insoluble in water, its application in daily chemical products is limited. The present invention improves the solubility of astaxanthin and expands its application range by preparing a nanostructured lipid carrier and introducing an inclusion compound hydroxypropyl-β-cyclodextrin.

[0028] In the present invention, the pale yellow-green volatile oil obtained by water distillation extraction of the aerial part of the Asteraceae plant Artemisia annua is one of the main effective parts of the traditional Chinese medicine Artemisia annua. It contains multiple chemical components such as artemisinin, isoartemisinin, eucalyptol, L-camphor, caryophyllene, pinene, etc., and has antibacterial, anti-inflammatory, antitussive and expectorant effects. However, it is volatile and unstable in nature, which limits its application. The present invention reduces its volatility, increases stability, and improves its application range by adding hydroxypropyl-β-cyclodextrin modified by hydroxypropyl chitosan.

[0029] Preferably, the concentration of the 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride solution in step S1 is 20wt%, the mass ratio of the succinic acid, deionized water, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride solution, hydroxypropyl chitosan, hydroxypropyl-β-cyclodextrin, and N-hydroxysuccinimide is 5-8:200-300:50-60:15-20:20-30:10-15, the temperature of the isothermal reaction is 80-90°C, and the time is 2-4h.

[0030] Preferably, in step S2, the mass ratio of the solid product, whey protein, Tween 80 and deionized water is 10-20:5-8:3-5:100-150; the mass ratio of astaxanthin, artemisia annua oil, glyceryl distearate and anhydrous ethanol is 1-2:10-15:20-30:50.

[0031] Preferably, the homogenization shear rate in step S3 is 2000-4000 r / min, the temperature is 75-80° C., and the time is 30-40 min.

[0032] The present invention also protects a method for preparing the scalp care solution containing the probiotic fermentation product as described above, comprising the following steps:

[0033] The raw materials are weighed according to the formula, glycerol and probiotic fermentation products are added into water, stirred at 60-70° C. for 20-30 min, then 1,2-hexanediol, p-hydroxyacetophenone, carbomer and arginine are added, and stirring is continued for 20-30 min. After cooling to room temperature, plant extracts, trace ingredients and composite nano-carrier liquid are added, stirred at 40-50° C. for 30-50 min, homogenized at 3000-4000 r / min for 5-10 min, and sterilized to obtain the scalp care solution containing probiotic fermentation products.

[0034] Compared with the prior art, the present invention has the following beneficial effects:

[0035] (1) The scalp care solution containing probiotic fermentation products provided by the present invention has the function of adjusting the flora environment of the scalp and improving the problems of severe oil secretion and dandruff and itching through the interaction of various raw materials in the formula. It also has the function of inhibiting Malassezia and solving the problem of scalp microecological imbalance caused by excessive Malassezia. It can also promote scalp repair and the formation of hair collagen, and improve the scalp microecology. The scalp care solution has good stability and has good application prospects in cosmetics.

[0036] (2) The scalp care solution containing probiotic fermentation products provided by the present invention comprises a probiotic fermentation product of lactobacillus fermentation product, a filtrate of bifid yeast fermentation product, an extract of altermonas fermentation product, and a lysate of bifid yeast fermentation product. Compared with a single probiotic fermentation product, the probiotic fermentation product has better antioxidant properties, moisturizing properties, and the function of regulating the scalp flora environment. The plant extract used is a complex of a matsutake extract, an astragalus root extract, and a melilotus extract. Compared with a single plant extract, the three plant extracts in the present invention have a better synergistic effect, further improving the anti-ultraviolet oxidation property, soothing scalp, and repairing damaged scalp of the scalp care solution. The present invention compounds the composite probiotic fermentation product with the composite plant extract, and simultaneously supplements with carbomer, arginine, trace ingredient raw materials, and a composite nano-carrier liquid, so that the ingredients in the care solution are more easily absorbed and utilized by the scalp, thereby improving the ecological microenvironment of the scalp.

[0037] (3) The scalp care solution containing probiotic fermentation products provided by the present invention, the composite nano-carrier solution added, firstly stir succinic acid and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride to activate, so as to increase the activity of the carboxyl group in succinic acid, and then add hydroxypropyl deacetylated chitosan and hydroxypropyl-β-cyclodextrin, the carboxyl group in succinic acid reacts with the hydroxyl group in hydroxypropyl deacetylated chitosan and hydroxypropyl-β-cyclodextrin, and the two are combined in the form of covalent bonds, thereby improving the stability of the two in the care solution, hydroxypropyl deacetylated chitosan has good antioxidant, antibacterial and anti-inflammatory properties, and also has certain moisturizing properties, and hydroxypropyl-β-cyclodextrin can be used as an inclusion compound to improve the solubility of astaxanthin and active substances in plant extracts that are difficult to dissolve in water, thereby increasing The utilization of water-insoluble active ingredients makes the care solution have better functions of resisting free radicals, moisturizing, repairing scalp and strengthening scalp barrier. Then, artemisia annua oil and glyceryl distearate are used as oil phase, whey protein and Tween 80 are used as surfactants, and nanostructured lipid carriers are prepared by high-speed stirring-ultrasonic dispersion method. Hydroxypropyl deacetylated chitosan-modified hydroxypropyl-β-cyclodextrin is added during the preparation process to improve the solubility of astaxanthin, increase the stability of astaxanthin and improve the bioavailability; at the same time, the artemisia annua oil in the composite nano-carrier liquid has a good penetration-promoting effect, which can promote the active ingredients in plant extracts (such as triterpenoids, flavonoids, coumarin compounds, etc.) to be absorbed and utilized by the scalp, realize the synergistic effect of multiple ingredients, and thus promote scalp health. DETAILED DESCRIPTION

[0038] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0039] The lactobacillus fermentation product was purchased from Jiangsu Jufeng Chemical Technology Co., Ltd.

[0040] The filtrate of the bifid yeast fermentation product was purchased from Hubei Langbowan Biopharmaceutical Co., Ltd.

[0041] The Alteromonas fermentation product extract was purchased from Shaanxi Xinkang Biotechnology Co., Ltd.

[0042] The bifid yeast fermentation product lysate was purchased from Jiangsu Caiwei Biotechnology Co., Ltd.

[0043] The Tricholoma matsutake extract was purchased from Lanzhou Waterless Biotechnology Co., Ltd., with a product specification of 10:1;

[0044] The Astragalus membranaceus root extract was purchased from Shaanxi Aibonyi Biotechnology Co., Ltd., with a product specification of 10:1;

[0045] The Melilotus officinalis extract was purchased from Xi'an Youshuo Biotechnology Co., Ltd., with a product specification of 10:1;

[0046] The hydroxypropyl deacetylated chitosan was purchased from Huzhou Purui Biopharmaceutical Technology Co., Ltd.

[0047] Example 1

[0048] A scalp care solution containing probiotic fermentation products, comprising the following raw materials:

[0049] 89.63 parts of water, 3 parts of glycerol, 5.5 parts of probiotic fermentation products, 2.1 parts of plant extracts, 0.55 parts of 1,2-hexanediol, 0.55 parts of p-hydroxyacetophenone, 0.2 parts of carbomer, 0.15 parts of arginine, 0.14 parts of trace ingredients, and 9 parts of composite nanocarrier liquid.

[0050] Among them, the probiotic fermentation product is composed of the following raw materials: 2.5 parts of lactobacillus fermentation product, 1.5 parts of bifid yeast fermentation product filtrate, 1.35 parts of altermonas fermentation product extract, and 0.15 parts of bifid yeast fermentation product lysate; the plant extract is composed of the following raw materials: 0.95 parts of matsutake extract, 0.55 parts of astragalus membranaceus root extract, and 0.6 parts of melilotus extract; the trace ingredients are composed of the following raw materials: 9 parts of 1,2-pentanediol, 5 parts of disodium EDTA, 0.1 parts of ethylhexylglycerol, 0.02 parts of ergothioneine, 0.02 parts of acetyl octapeptide-3, and 0.01 parts of arginine / lysine polypeptide.

[0051] The preparation method of the composite nano-carrier liquid is as follows:

[0052] S1. Add 7g of succinic acid to 250g of deionized water, stir to dissolve, then add 55g of 20wt% 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride solution, stir and activate at 40°C for 1h, then add 18g of hydroxypropyl deacetylated chitosan, 25g of hydroxypropyl-β-cyclodextrin, and 12g of N-hydroxysuccinimide, and react at 85°C for 3h. After the reaction is completed, add 4 times the volume of dichloromethane to precipitate, filter, wash with ethanol, and dry at 80°C for 4h to obtain a solid product;

[0053] S2, adding 15g of the solid product in step S1, 6g of whey protein, and 4g of Tween 80 to 130g of deionized water, stirring evenly to obtain an aqueous phase; adding 1.5g of astaxanthin, 13g of artemisia annua oil, and 25g of glyceryl distearate to 50g of anhydrous ethanol, stirring at 75°C to obtain an oil phase;

[0054] S3, the oil phase in step S2 is added to the water phase, and homogenized with a high-speed shear emulsifier at a shear rate of 3000r / min, a temperature of 75°C, and a time of 35min to disperse and form an emulsion solution, and the water bath temperature is maintained at 75°C, and high-speed stirring is continued to evaporate the ethanol, and 50mL of deionized water at the same temperature is added to the emulsion, and then the emulsion is treated with an ultrasonic power of 400W, and the ultrasonic frequency is: ultrasonic 5s, interval 5s, and continuous ultrasonic 7min. After the ultrasonic end, the emulsion is placed in an ice water bath to cool, and impurities are filtered through a 0.8μm filter membrane to obtain.

[0055] The method for preparing the scalp care solution containing the probiotic fermentation product comprises the following steps:

[0056] Weigh the raw materials according to the above formula, add glycerol and probiotic fermentation products into water, stir at 65°C for 25 minutes, then add 1,2-hexanediol, p-hydroxyacetophenone, carbomer, and arginine, continue stirring for 25 minutes, cool to room temperature, add plant extracts, trace ingredients, and composite nano-carrier liquid, stir at 45°C for 40 minutes, homogenize at 3500r / min for 8 minutes, and sterilize to obtain the scalp care solution containing probiotic fermentation products.

[0057] Example 2

[0058] A scalp care solution containing probiotic fermentation products, comprising the following raw materials:

[0059] 89 parts of water, 4 parts of glycerin, 6.5 parts of probiotic fermentation products, 1.9 parts of plant extracts, 0.6 parts of 1,2-hexanediol, 0.6 parts of p-hydroxyacetophenone, 0.2 parts of carbomer, 0.15 parts of arginine, 0.12 parts of trace ingredients, and 8 parts of composite nanocarrier liquid.

[0060] Among them, the probiotic fermentation product is composed of the following raw materials: 3 parts of lactobacillus fermentation product, 2 parts of bifid yeast fermentation product filtrate, 1.3 parts of altermonas fermentation product extract, and 0.2 parts of bifid yeast fermentation product lysate; the plant extract is composed of the following raw materials: 0.7 parts of matsutake extract, 0.5 parts of astragalus membranaceus root extract, and 0.7 parts of melilotus extract; the trace ingredients are composed of the following raw materials: 9.5 parts of 1,2-pentanediol, 5.5 parts of disodium EDTA, 0.2 parts of ethylhexylglycerin, 0.02 parts of ergothioneine, 0.02 parts of acetyl octapeptide-3, and 0.015 parts of arginine / lysine polypeptide.

[0061] The preparation method of the composite nano-carrier liquid is as follows:

[0062] S1. Add 6 g of succinic acid to 250 g of deionized water, stir to dissolve, then add 55 g of 20 wt% 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride solution, stir and activate at 40 ° C for 1 h, then add 17 g of hydroxypropyl deacetylated chitosan, 25 g of hydroxypropyl-β-cyclodextrin, and 12 g of N-hydroxysuccinimide, and react at 85 ° C for 3 h. After the reaction is completed, add 4 times the volume of dichloromethane to precipitate, filter, wash with ethanol, and dry at 80 ° C for 4 h to obtain a solid product;

[0063] S2, adding 15g of the solid product in step S1, 6g of whey protein, and 4g of Tween 80 to 120g of deionized water, stirring evenly to obtain an aqueous phase; adding 1.5g of astaxanthin, 12g of artemisia annua oil, and 25g of glyceryl distearate to 50g of anhydrous ethanol, stirring at 75°C to obtain an oil phase;

[0064] S3, the oil phase in step S2 is added to the water phase, and homogenized with a high-speed shear emulsifier at a shear rate of 3000r / min, a temperature of 80°C, and a time of 35min to disperse and form an emulsion solution, and the water bath temperature is maintained at 80°C, and high-speed stirring is continued to evaporate the ethanol, and 50mL of deionized water at the same temperature is added to the emulsion, and then the emulsion is treated with an ultrasonic power of 400W, and the ultrasonic frequency is: ultrasonic 5s, interval 5s, and continuous ultrasonic 6min. After the ultrasonic end, the emulsion is placed in an ice water bath to cool, and impurities are filtered through a 0.8μm filter membrane to obtain.

[0065] The method for preparing the scalp care solution containing the probiotic fermentation product comprises the following steps:

[0066] Weigh the raw materials according to the above formula, add glycerol and probiotic fermentation products into water, stir at 65°C for 25 minutes, then add 1,2-hexanediol, p-hydroxyacetophenone, carbomer, and arginine, continue stirring for 25 minutes, cool to room temperature, add plant extracts, trace ingredients, and composite nano-carrier liquid, stir at 45°C for 40 minutes, homogenize at 3500r / min for 7 minutes, and sterilize to obtain the scalp care solution containing probiotic fermentation products.

[0067] Example 3

[0068] A scalp care solution containing probiotic fermentation products, comprising the following raw materials:

[0069] 87 parts of water, 3 parts of glycerol, 4 parts of probiotic fermentation products, 1.3 parts of plant extracts, 0.4 parts of 1,2-hexanediol, 0.4 parts of p-hydroxyacetophenone, 0.1 parts of carbomer, 0.1 parts of arginine, 0.1 parts of trace ingredients, and 7 parts of composite nanocarrier liquid.

[0070] Among them, the probiotic fermentation product is composed of the following raw materials: 2 parts of lactobacillus fermentation product, 1 part of bifid yeast fermentation product filtrate, 0.9 parts of altermonas fermentation product extract, and 0.1 parts of bifid yeast fermentation product lysate; the plant extract is composed of the following raw materials: 0.5 parts of matsutake extract, 0.3 parts of astragalus membranaceus root extract, and 0.5 parts of melilotus extract; the trace ingredients are composed of the following raw materials: 9 parts of 1,2-pentanediol, 5 parts of disodium EDTA, 0.1 parts of ethylhexylglycerin, 0.01 parts of ergothioneine, 0.01 parts of acetyl octapeptide-3, and 0.01 parts of arginine / lysine polypeptide.

[0071] The preparation method of the composite nano-carrier liquid is as follows:

[0072] S1. Add 5 g of succinic acid to 200 g of deionized water, stir to dissolve, then add 50 g of 20 wt% 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride solution, stir and activate at 40°C for 1 h, then add 15 g of hydroxypropyl deacetylated chitosan, 20 g of hydroxypropyl-β-cyclodextrin, and 10 g of N-hydroxysuccinimide, and react at 80°C for 4 h. After the reaction is completed, add 4 times the volume of dichloromethane to precipitate, filter, wash with ethanol, and dry at 80°C for 4 h to obtain a solid product;

[0073] S2, adding 10g of the solid product in step S1, 5g of whey protein, and 3g of Tween 80 to 100g of deionized water, stirring evenly to obtain an aqueous phase; adding 1g of astaxanthin, 10g of artemisia annua oil, and 20g of glyceryl distearate to 50g of anhydrous ethanol, stirring at 75°C to obtain an oil phase;

[0074] S3, the oil phase in step S2 is added to the water phase, and homogenized with a high-speed shear emulsifier at a shear rate of 2000r / min, a temperature of 75°C, and a time of 20min to disperse and form an emulsion solution, and the water bath temperature is maintained at 75°C, and high-speed stirring is continued to evaporate the ethanol, and 50mL of deionized water at the same temperature is added to the emulsion, and then the emulsion is treated with an ultrasonic power of 400W, and the ultrasonic frequency is: ultrasonic 5s, interval 5s, and continuous ultrasonic 5min. After the ultrasonic end, the emulsion is placed in an ice water bath to cool, and impurities are filtered through a 0.8μm filter membrane to obtain.

[0075] The method for preparing the scalp care solution containing the probiotic fermentation product comprises the following steps:

[0076] Weigh the raw materials according to the above formula, add glycerol and probiotic fermentation products into water, stir at 60°C for 30 minutes, then add 1,2-hexanediol, p-hydroxyacetophenone, carbomer, and arginine, continue stirring for 20 minutes, cool to room temperature, add plant extracts, trace ingredients, and composite nano-carrier liquid, stir at 40°C for 50 minutes, homogenize at 3000r / min for 10 minutes, and sterilize to obtain the scalp care solution containing probiotic fermentation products.

[0077] Example 4

[0078] A scalp care solution containing probiotic fermentation products, comprising the following raw materials:

[0079] 91 parts of water, 5 parts of glycerin, 9.3 parts of probiotic fermentation products, 2.6 parts of plant extracts, 0.8 parts of 1,2-hexanediol, 0.8 parts of p-hydroxyacetophenone, 0.3 parts of carbomer, 0.2 parts of arginine, 0.15 parts of trace ingredients, and 11 parts of composite nanocarrier liquid.

[0080] Among them, the probiotic fermentation product is composed of the following raw materials: 4 parts of lactobacillus fermentation product, 3 parts of bifid yeast fermentation product filtrate, 2 parts of altermonas fermentation product extract, and 0.3 parts of bifid yeast fermentation product lysate; the plant extract is composed of the following raw materials: 1 part of matsutake extract, 0.7 parts of astragalus membranaceus root extract, and 0.9 parts of melilotus extract; the trace ingredients are composed of the following raw materials: 10 parts of 1,2-pentanediol, 6 parts of disodium EDTA, 0.3 parts of ethylhexylglycerol, 0.04 parts of ergothioneine, 0.03 parts of acetyl octapeptide-3, and 0.02 parts of arginine / lysine polypeptide.

[0081] The preparation method of the composite nano-carrier liquid is as follows:

[0082] S1. Add 8 g of succinic acid to 300 g of deionized water, stir to dissolve, then add 60 g of 20 wt% 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride solution, stir and activate at 40°C for 1 h, then add 20 g of hydroxypropyl deacetylated chitosan, 30 g of hydroxypropyl-β-cyclodextrin, and 15 g of N-hydroxysuccinimide, and react at 90°C for 2 h. After the reaction is completed, add 4 times the volume of dichloromethane to precipitate, filter, wash with ethanol, and dry at 80°C for 4 h to obtain a solid product;

[0083] S2, adding 20g of the solid product in step S1, 8g of whey protein, and 5g of Tween 80 to 150g of deionized water, stirring evenly to obtain an aqueous phase; adding 2g of astaxanthin, 15g of artemisia annua oil, and 30g of glyceryl distearate to 50g of anhydrous ethanol, stirring at 75°C to obtain an oil phase;

[0084] S3, the oil phase in step S2 is added to the water phase, and homogenized with a high-speed shear emulsifier at a shear rate of 4000r / min, a temperature of 80°C, and a time of 10min to disperse and form an emulsion solution, and the water bath temperature is maintained at 80°C, and high-speed stirring is continued to evaporate the ethanol, and 50mL of deionized water at the same temperature is added to the emulsion, and then the emulsion is treated with an ultrasonic power of 400W, and the ultrasonic frequency is: ultrasonic 5s, interval 5s, and continuous ultrasonic 8min. After the ultrasonic end, the emulsion is placed in an ice water bath to cool, and impurities are filtered through a 0.8μm filter membrane to obtain.

[0085] The method for preparing the scalp care solution containing the probiotic fermentation product comprises the following steps:

[0086] Weigh the raw materials according to the above formula, add glycerol and probiotic fermentation products into water, stir at 70°C for 20 minutes, then add 1,2-hexanediol, p-hydroxyacetophenone, carbomer, and arginine, continue stirring for 30 minutes, cool to room temperature, add plant extracts, trace ingredients, and composite nano-carrier liquid, stir at 50°C for 30 minutes, homogenize at 4000r / min for 5 minutes, and sterilize to obtain the scalp care solution containing probiotic fermentation products.

[0087] Comparative Example 1

[0088] The only difference from Example 1 is that the probiotic fermentation product in the scalp care solution is a lactobacillus fermentation product.

[0089] Comparative Example 2

[0090] The only difference from Example 1 is that the probiotic fermentation product in the scalp care solution is a filtrate of a bifida fermentation product.

[0091] Comparative Example 3

[0092] The only difference from Example 1 is that the probiotic fermentation product in the scalp care solution is a lysate of bifida fermentation product.

[0093] Comparative Example 4

[0094] The only difference from Example 1 is that the plant extract in the scalp care solution is the Astragalus membranaceus root extract.

[0095] Comparative Example 5

[0096] The only difference from Example 1 is that the plant extract in the scalp care solution is Melilotus officinalis extract.

[0097] Comparative Example 6

[0098] The only difference from Example 1 is that the plant extract in the scalp care solution is a Tricholoma matsutake extract.

[0099] Comparative Example 7

[0100] The only difference from Example 1 is that the scalp care solution does not contain the composite nano-carrier liquid.

[0101] 1. Stability test of scalp care solution

[0102] In order to show that the scalp care solution prepared by the present invention has a stable formula system, the scalp care solutions prepared by Examples 1-4 and Comparative Examples 1-7 were tested for stability, and the results are shown in Table 1:

[0103] Table 1

[0104]

[0105] As can be seen from Table 1 above, the scalp care solution containing probiotic fermentation products prepared by the present invention has good stability after standing for 120 days and in high and low temperature environments. The care solution is clear, not turbid, and has no precipitation. However, since the composite nano-carrier liquid is not added in Comparative Example 7, the stability of the care solution prepared is poor.

[0106] 2. Antioxidant DPPH free radical scavenging test

[0107] Test materials: scalp care solutions prepared in Examples 1-4 and Comparative Examples 1-7

[0108] Test method: 1 mL of the scalp care solution prepared in Examples 1-4 and Comparative Examples 1-7 was added to 10 mL of a 1×10 -4 mol / L DPPH solution, mix thoroughly, and immediately test the absorbance at 518nm and record it as A1. Then, place it at room temperature and avoid light for 30 minutes, and then measure the absorbance and record it as A2. The final concentration is 1×10 -4 The absorbance value of the DPPH solution of 10 mol / L is recorded as A0, and the scavenging rate of DPPH free radicals is calculated according to the following formula: scavenging rate = [1-(A1-A2) / A0] × 100%; the scalp care solutions in Examples 1-4 and Comparative Examples 1-7 were placed under natural light conditions for 7 weeks, and then the scavenging rates of DPPH free radicals were tested according to the above method. The test results are shown in Table 2:

[0109] Table 2

[0110] DPPH free radical scavenging rate / % DPPH free radical scavenging rate after 7 weeks / % Example 1 76.2 75.3 Example 2 75.6 74.4 Example 3 73.9 72.5 Example 4 78.1 76.4 Comparative Example 1 62.4 60.3 Comparative Example 2 64.8 63.2 Comparative Example 3 65.1 62.7 Comparative Example 4 63.7 61.5 Comparative Example 5 66.3 63.6 Comparative Example 6 61.9 60.1 Comparative Example 7 68.5 59.2

[0111] It can be seen from Table 2 above that the scalp care solution containing the probiotic fermentation product prepared by the present invention has excellent DPPH free radical scavenging ability, and the antioxidant property does not decrease significantly after storage for 7 weeks, and has good stability; while in Comparative Examples 1-6, since the probiotic fermentation product and the plant extract are both single raw materials, their antioxidant property and stability are significantly reduced.

[0112] 3. Testing of the efficacy of scalp flora environment

[0113] The scalp care solutions prepared in Example 1 and Comparative Examples 1-7 were tested for their efficacy in adjusting the flora environment of the scalp. The specific experimental method was as follows: Staphylococcus epidermidis, Propionibacterium acnes and Malassezia were inoculated in glass culture dishes and conventional culture medium, respectively. The culture medium used was agar medium, and the inoculation amount was 1×10 4 Then, scalp care solution was added to the inoculated surface, the culture temperature was 30°C, the culture speed was 150rpm, and the number of colonies was detected after 24 hours. The test results are shown in Table 3:

[0114] Table 3

[0115] Staphylococcus epidermidis Propionibacterium acnes Malassezia Example 1 <![CDATA[4.59×10 6 ]]> <![CDATA[5.17×10 4 ]]> <![CDATA[3.22×10 4 ]]> Comparative Example 1 <![CDATA[8.27×10 5 ]]> <![CDATA[7.81×10 4 ]]> <![CDATA[5.26×10 4 ]]> Comparative Example 2 <![CDATA[6.14×10 5 ]]> <![CDATA[7.06×10 4 ]]> <![CDATA[5.87×10 4 ]]> Comparative Example 3 <![CDATA[5.81×10 5 ]]> <![CDATA[9.36×10 4 ]]> <![CDATA[1.33×10 5 ]]> Comparative Example 4 <![CDATA[9.44×10 5 ]]> <![CDATA[1.08×10 5 ]]> <![CDATA[6.34×10 4 ]]> Comparative Example 5 <![CDATA[7.52×10 5 ]]> <![CDATA[8.73×10 4 ]]> <![CDATA[1.12×10 5 ]]> Comparative Example 6 <![CDATA[6.83×10 5 ]]> <![CDATA[1.54×10 5 ]]> <![CDATA[1.26×10 5 ]]> Comparative Example 7 <![CDATA[2.52×10 6 ]]> <![CDATA[7.52×10 4 ]]> <![CDATA[4.67×10 4 ]]>

[0116] It can be seen from the data in Table 3 that the scalp care solution containing probiotic fermentation products prepared by the present invention has the effect of promoting the growth of Staphylococcus epidermidis; at the same time, it has a significant inhibitory effect on the growth of Propionibacterium acnes and Malassezia, and has the effect of regulating the balance of scalp microecology.

[0117] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A scalp care solution containing probiotic fermentation products, characterized in that: By weight, it includes the following raw materials: 87-91 parts of water, 3-5 parts of glycerol, 4-9.3 parts of probiotic fermentation products, 1.3-2.6 parts of plant extracts, 0.4-0.8 parts of 1,2-hexanediol, 0.4-0.8 parts of p-hydroxyacetophenone, 0.1-0.3 parts of carbomer, 0.1-0.2 parts of arginine, 0.1-0.15 parts of trace ingredients, and 7-11 parts of composite nanocarrier liquid; The probiotic fermentation product is composed of the following raw materials: 2-4 parts of lactobacillus fermentation product, 1-3 parts of bifid yeast fermentation product filtrate, 0.9-2 parts of altermonas fermentation product extract, and 0.1-0.3 parts of bifid yeast fermentation product lysate; The plant extract is composed of the following raw materials: 0.5-1 part of Tricholoma matsutake extract, 0.3-0.7 part of Astragalus membranaceus root extract, and 0.5-0.9 part of Melilotifolia extract; The trace components are composed of the following raw materials: 9-10 parts of 1,2-pentanediol, 5-6 parts of disodium EDTA, 0.1-0.3 parts of ethylhexylglycerol, 0.01-0.04 parts of ergothioneine, 0.01-0.03 parts of acetyl octapeptide-3, and 0.01-0.02 parts of arginine / lysine polypeptide; The preparation method of the composite nano-carrier liquid is as follows: S1. Add succinic acid into deionized water, stir to dissolve, then add 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride solution, stir to activate, then add hydroxypropyl chitosan, hydroxypropyl-β-cyclodextrin, and N-hydroxysuccinimide, and react at a constant temperature. After the reaction is completed, add 4 times the volume of dichloromethane for precipitation, filter, wash with ethanol, and dry to obtain a solid product; S2, adding the solid product in step S1, whey protein, and Tween 80 into deionized water, stirring evenly to obtain an aqueous phase; adding astaxanthin, artemisia annua oil, and glyceryl distearate into anhydrous ethanol, stirring at 75° C. to obtain an oil phase; S3, adding the oil phase in step S2 to the water phase, homogenizing with a high-speed shear emulsifier, dispersing to form an emulsion solution, and then subjecting to ultrasonic treatment. After the treatment is completed, cooling, filtering and removing impurities are performed to obtain.

2. The scalp care solution containing probiotic fermentation products according to claim 1, characterized in that: By weight, it includes the following raw materials: 88-90 parts of water, 3-4 parts of glycerol, 5-7 parts of probiotic fermentation products, 1.3-2.6 parts of plant extracts, 0.4-0.6 parts of 1,2-hexanediol, 0.4-0.6 parts of p-hydroxyacetophenone, 0.1-0.2 parts of carbomer, 0.15-0.2 parts of arginine, 0.12-0.15 parts of trace ingredients, and 8-10 parts of composite nano-carrier liquid.

3. The scalp care solution containing probiotic fermentation products according to claim 1, characterized in that: The invention comprises the following raw materials by weight: 89.63 parts of water, 3 parts of glycerol, 5.5 parts of probiotic fermentation products, 2.1 parts of plant extracts, 0.55 parts of 1,2-hexanediol, 0.55 parts of p-hydroxyacetophenone, 0.2 parts of carbomer, 0.15 parts of arginine, 0.14 parts of trace ingredients and 9 parts of composite nano-carrier liquid.

4. The scalp care solution containing probiotic fermentation products according to claim 1, characterized in that: The concentration of the 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride solution in step S1 is 20wt%, the mass ratio of the succinic acid, deionized water, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride solution, hydroxypropyl chitosan, hydroxypropyl-β-cyclodextrin, and N-hydroxysuccinimide is 5-8:200-300:50-60:15-20:20-30:10-15, the temperature of the isothermal reaction is 80-90°C, and the time is 2-4h.

5. The scalp care solution containing probiotic fermentation products according to claim 1, characterized in that: In step S2, the mass ratio of the solid product, whey protein, Tween 80 and deionized water is 10-20:5-8:3-5:100-150; the mass ratio of astaxanthin, artemisia annua oil, glyceryl distearate and anhydrous ethanol is 1-2:10-15:20-30:

50.

6. The scalp care solution containing probiotic fermentation products according to claim 1, characterized in that: The shear rate of the homogenization in step S3 is 2000-4000 r / min, the temperature is 75-80° C., and the time is 30-40 min; the power of the ultrasonic treatment is 400 W, and the time is 5-8 min.

7. A method for preparing a scalp care solution containing a probiotic fermentation product according to any one of claims 1 to 6, characterized in that: The method comprises the following steps: weighing raw materials according to the formula, adding glycerin and probiotic fermentation products into water, stirring at 60-70°C for 20-30 minutes, then adding 1,2-hexanediol, p-hydroxyacetophenone, carbomer, and arginine, continuing stirring for 20-30 minutes, and adding plant extracts, trace ingredients, and composite nano-carrier liquid after cooling to room temperature, stirring at 40-50°C for 30-50 minutes, homogenizing at 3000-4000r / min for 5-10 minutes, and sterilizing to obtain the scalp care solution containing probiotic fermentation products.

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