Anti-aging composition containing PQQ and preparation method thereof

Abstract

The invention discloses an anti-aging composition containing PQQ and a preparation method of the anti-aging composition, and belongs to the field of medicines or health food, the anti-aging composition comprises PQQ, an antioxidant, collagen, hyaluronic acid and a plant extract, and the content of PQQ is 0.1-5%; 1%-10% of collagen; 0.05%-2% of hyaluronic acid; the antioxidant comprises the following components in percentage by weight: 0.5%-5% of vitamin C, 0.1%-3% of alpha-tocopherol and 0.05%-2% of beta-carotene; the composition contains PQQ and various antioxidants such as vitamin C, alpha-tocopherol, beta-carotene, coenzyme Q10 and glutathione, and the components have a synergistic effect, so that free radicals can be effectively removed, oxidative damage can be reduced, and cell aging can be delayed; most of the components in the composition are natural substances or are widely applied to the fields of food and health care products, the safety is high, and the quality and safety of the product can be ensured through the steps of strict preparation process control, accurate component dissolving method, pH value adjustment, filtration and the like.

Description

Technical Field

[0001] The present invention relates to the technical field of medicines or health food, and in particular to an anti-aging composition containing PQQ and a preparation method thereof. Background Art

[0002] PQQ (pyrroloquinoline quinone) is a coenzyme of oxidoreductase. PQQ is a strong antioxidant that can effectively remove free radicals in the body. By removing free radicals and protecting mitochondria, PQQ can delay the aging process of cells.

[0003] As people's living standards improve and their attention to health and beauty continues to increase, anti-aging has become a hot research field. There are many anti-aging products on the market, but most of them are not ideal or have certain side effects. Therefore, a safe and effective anti-aging composition containing PQQ is needed. Summary of the invention

[0004] In view of the deficiencies of the prior art, the present invention provides an anti-aging composition containing PQQ and a preparation method thereof, which solves the problems raised in the above background technology.

[0005] Technical solution: To solve the above technical problems, according to one aspect of the present invention, more specifically, an anti-aging composition containing PQQ, including PQQ, antioxidants, collagen, hyaluronic acid, and plant extracts, wherein the content of PQQ is 0.1%-5%; collagen is 1%-10%; and hyaluronic acid is 0.05%-2%;

[0006] The antioxidants include 0.5%-5% vitamin C, 0.1%-3% α-tocopherol, 0.05%-2% β-carotene, 0.05%-2% coenzyme Q10, and 0.05%-2% glutathione;

[0007] The plant extracts include grape seed extract with a content of 0.5%-5%, green tea extract with a content of 0.5%-4%, and ginseng extract with a content of 0.1%-3%.

[0008] Furthermore, the grape seed extract contains proanthocyanidins, catechins, epicatechin polyphenols; the green tea extract is rich in tea polyphenols, including catechins, flavonoids, and anthocyanidins; and the ginseng extract includes a variety of ginsenosides, polysaccharides, and flavonoids.

[0009] According to another aspect of the present invention, more specifically, a method for preparing an anti-aging composition containing PQQ comprises the following steps:

[0010] S1. Raw material preparation: prepare PQQ, antioxidants, collagen, hyaluronic acid, and plant extract raw materials according to the formula ratio;

[0011] Prepare purified water, ethanol, and acid-base regulator;

[0012] S2. Dissolution of ingredients:

[0013] a. Dissolution of PQQ: slowly add PQQ powder into an appropriate amount of solvent. You can first use a small amount of ethanol or other organic solvent to help dissolve it, and then dilute it with pure water to the required concentration; stir evenly to ensure that PQQ is completely dissolved;

[0014] Pay attention to controlling the dissolution temperature to avoid excessively high temperatures affecting the stability of PQQ; the dissolution operation can generally be carried out between room temperature and 40°C;

[0015] b. Dissolution of antioxidants:

[0016] Vitamin C can be directly dissolved in pure water. Use 15 ml of water for every gram of vitamin C. Stir evenly at a stirring speed of 200-500 rpm.

[0017] α-tocopherol, add ethanol according to the ratio of α-tocopherol to ethanol of about 1:10, stir at a speed of 100-300 rpm, and then dilute with purified water;

[0018] β-carotene, add vegetable oil according to the ratio of β-carotene to vegetable oil of about 1:20, use ultrasound to assist dissolution, the ultrasound power can be set to 200-400W, and the ultrasound time is 20 minutes;

[0019] Add ethanol at a ratio of about 1:10; stir at a speed of 150-400 rpm for more than 30 minutes;

[0020] Add glutathione to the solvent at a ratio of about 1:10, stirring at a speed of 200-500 rpm for 10-30 minutes;

[0021] The room temperature during the above dissolution process is controlled at 20-25°C;

[0022] c. Dissolution of collagen:

[0023] Add the collagen powder to an appropriate amount of pure water and stir slowly at a low temperature of 4°C to allow it to gradually dissolve;

[0024] d. Dissolution of hyaluronic acid:

[0025] Hyaluronic acid can be directly dissolved in pure water, stirred evenly, and heated to about 40°C to speed up the dissolution;

[0026] e. Dissolution of plant extracts:

[0027] Add the solvent in a ratio of grape seed extract to solvent of about 1:10;

[0028] Green tea extract uses 70-90°C hot water as a solvent, adds hot water in a ratio of about 1:10 of green tea extract to hot water, stirs at a speed of 200-400 rpm, maintains the temperature at 70-90°C, and continues stirring for 15-30 minutes;

[0029] Add the solvent in a ratio of about 1:10 of ginseng extract to solvent, stir at a speed of 300-500 rpm, stir for 10-20 minutes at room temperature of 20-25°C, then raise the temperature to 40-50°C and continue stirring for 10-20 minutes;

[0030] S3, mixing: dissolving PQQ and other raw materials in appropriate amounts of solvents respectively, and then mixing the solutions together and stirring evenly;

[0031] S4. Adjusting pH value: A pH value of the composition can be adjusted using an acid-base regulator so that the pH value can be adjusted between 5 and 7;

[0032] S5. Filtration: Filter the mixed solution using a filter membrane with a pore size of 0.22 μm to remove impurities and insoluble particles;

[0033] S6. Filling: Fill the filtered solution into appropriate containers and store them in sealed containers.

[0034] Furthermore, the plant extract preparation method is as follows:

[0035] Raw material processing: the plant raw materials are pre-processed by crushing, drying, etc. to increase the surface area and improve the extraction efficiency;

[0036] Extraction: Select a suitable solvent, mix the plant material and the solvent in a certain proportion, extract at an appropriate temperature and time, and use the immersion method for extraction;

[0037] Filtration: Filter the extract to remove solid impurities;

[0038] Concentration: The solvent in the extract is removed by evaporation or other methods to obtain a concentrated plant extract.

[0039] Furthermore, the preparation method of PQQ is as follows:

[0040] Using 5-methyl-2,4-dinitroanisole as the starting material:

[0041] The first step: 5-methyl-2,4-dinitroanisole and diethyl oxalate undergo condensation reaction under the action of a base to prepare ethyl 3-(5-methoxy-2,4-dinitro)phenylpyruvate;

[0042] Step 2: 3-(5-methoxy-2,4-dinitro)phenylpyruvate undergoes a reduction reaction under the action of a reducing agent and then undergoes a ring closure to obtain 6-amino-5-methoxy-1H-indole-2-carboxylic acid ethyl ester; the reducing agent is hydrazine hydrate / RaneyNi, hydrazine hydrate-activated carbon / (FeCl3+AlCl3), 10% Pd-C / H2;

[0043] Step 3: 6-amino-5-methoxy-1H-indole-2-carboxylic acid ethyl ester reacts with glyoxylate and pyruvate in an oxygen atmosphere under the action of Lewis acid catalyst to generate 5-methoxy-1H-pyrrole (2,3-f) quinoline-2,7,9-tricarboxylate;

[0044] Step 4: 5-methoxy-1H-pyrrole (2,3-f) quinoline-2,7,9-tricarboxylate undergoes ester hydrolysis reaction to obtain 5-methoxy-1H-pyrrole (2,3-f) quinoline-2,7,9-tricarboxylic acid;

[0045] Step 5: 5-methoxy-1H-pyrrole (2,3-f) quinoline-2,7,9-tricarboxylic acid undergoes oxidation reaction under the action of oxidant Ce (NH4) 2 (NO3) 6 to obtain PQQ.

[0046] The beneficial effects of the anti-aging composition containing PQQ and the preparation method thereof of the present invention are:

[0047] (1) The composition contains PQQ and a variety of antioxidants such as vitamin C, α-tocopherol, β-carotene, coenzyme Q10 and glutathione. These ingredients work synergistically to effectively remove free radicals, reduce oxidative damage, and thus delay cell aging.

[0048] (2) Most of the ingredients in the composition of the present invention are natural substances or have been widely used in the field of food and health products, and are relatively safe. The quality and safety of the product can be ensured through strict preparation process control, precise ingredient dissolution method, pH adjustment and filtration steps;

[0049] PQQ can stimulate the biogenesis of mitochondria, that is, promote the formation of new mitochondria. Antioxidants composed of vitamin C, α-tocopherol and other ingredients help maintain the normal structure and function of mitochondria by neutralizing free radicals and reducing oxidative damage. It has mitochondrial repair function and plays a role in protecting mitochondria from damage, maintaining normal mitochondrial function and promoting mitochondrial biogenesis in a direct or indirect way, thereby facilitating the mitochondrial repair process. BRIEF DESCRIPTION OF THE DRAWINGS

[0050] The present invention is further described in detail below with reference to the accompanying drawings and specific implementation methods.

[0051] Figure 1 It is a structural schematic diagram of the present invention. DETAILED DESCRIPTION

[0052] The present invention will be described in detail below with reference to the accompanying drawings and in combination with embodiments. It should be noted that the embodiments and features in the embodiments of the present application can be combined with each other without conflict.

[0053] In order to make the technical solution of the present invention clearer, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0054] Reference Figure 1 , an anti-aging composition containing PQQ, comprising PQQ, antioxidants, collagen, hyaluronic acid, and plant extracts, wherein the content of PQQ is 0.1%-5%; collagen is 1%-10%; and hyaluronic acid is 0.05%-2%;

[0055] The antioxidants include 0.5%-5% vitamin C, 0.1%-3% α-tocopherol, 0.05%-2% β-carotene, 0.05%-2% coenzyme Q10, and 0.05%-2% glutathione;

[0056] Preferably, the plant extract includes 0.5%-5% grape seed extract, 0.5%-4% green tea extract, and 0.1%-3% ginseng extract.

[0057] The grape seed extract contains proanthocyanidins, catechins, epicatechin polyphenols; the green tea extract is rich in tea polyphenols, including catechins, flavonoids, and anthocyanidins; and the ginseng extract includes a variety of ginsenosides, polysaccharides, and flavonoids.

[0058] Reference Figure 1 , a method for preparing an anti-aging composition containing PQQ, comprising the following steps:

[0059] S1. Raw material preparation: prepare PQQ, antioxidants, collagen, hyaluronic acid, and plant extract raw materials according to the formula ratio;

[0060] Prepare purified water, ethanol, and acid-base regulators (such as sodium hydroxide, hydrochloric acid, etc.);

[0061] S2. Dissolution of ingredients:

[0062] a. Dissolution of PQQ: slowly add PQQ powder into an appropriate amount of solvent. You can first use a small amount of ethanol or other organic solvent to help dissolve it, and then dilute it with pure water to the required concentration; stir evenly to ensure that PQQ is completely dissolved;

[0063] Pay attention to controlling the dissolution temperature to avoid excessively high temperatures affecting the stability of PQQ; the dissolution operation can generally be carried out between room temperature and 40°C;

[0064] b. Dissolution of antioxidants:

[0065] Vitamin C can be directly dissolved in pure water (15 ml of water can be used for each gram of vitamin C), stirred evenly, and the stirring speed is controlled at 200-500 rpm;

[0066] α-tocopherol, adding ethanol in a ratio of α-tocopherol to ethanol of about 1:10-1:20 (mass to volume ratio), stirring at a speed of 100-300 rpm, and then diluting with purified water;

[0067] β-carotene, adding vegetable oil according to a ratio of β-carotene to vegetable oil of about 1:20-1:50 (mass to volume ratio), using ultrasound to assist dissolution, the ultrasound power can be set to 200-400W, and the ultrasound time is 20 minutes;

[0068] Add ethanol at a ratio of about 1:10-1:30 (mass to volume ratio) of coenzyme Q10 to ethanol; stir at a speed of 150-400 rpm for more than 30 minutes;

[0069] Glutathione is added to the solvent (water or buffer) at a ratio of about 1:10-1:30 (mass to volume ratio), with a stirring speed of 200-500 rpm for 10-30 minutes;

[0070] The room temperature during the above dissolution process is controlled at 20-25°C;

[0071] c. Dissolution of collagen:

[0072] Add the collagen powder into an appropriate amount of pure water and stir slowly at low temperature (around 4°C) to gradually dissolve it.

[0073] d. Dissolution of hyaluronic acid:

[0074] Hyaluronic acid can be directly dissolved in pure water, stirred evenly, and heated to about 40°C to speed up the dissolution;

[0075] e. Dissolution of plant extracts:

[0076] Add the solvent in a ratio of grape seed extract to solvent of about 1:10-1:20 (mass to volume ratio);

[0077] Green tea extract uses 70-90°C hot water as a solvent, adds hot water in a ratio of about 1:10-1:30 (mass to volume ratio) of green tea extract to hot water, stirs at a speed of 200-400 rpm, maintains the temperature at 70-90°C, and continues stirring for 15-30 minutes;

[0078] Add the solvent in a ratio of about 1:10-1:20 (mass-volume ratio) of ginseng extract to solvent, stir at a speed of 300-500 rpm, stir for 10-20 minutes at room temperature (20-25°C), then raise the temperature to 40-50°C and continue stirring for 10-20 minutes;

[0079] S3, mixing: dissolving PQQ and other raw materials in appropriate amounts of solvents respectively, and then mixing the solutions together and stirring evenly;

[0080] S4. Adjusting pH value: A pH value of the composition can be adjusted using an acid-base regulator so that the pH value can be adjusted between 5 and 7;

[0081] S5. Filtration: Filter the mixed solution using a filter membrane with a pore size of 0.22 μm to remove impurities and insoluble particles;

[0082] S6. Filling: Fill the filtered solution into appropriate containers and store them in sealed containers.

[0083] Preferably, the plant extract preparation method is as follows:

[0084] Raw material processing: the plant raw materials are pre-processed by crushing, drying, etc. to increase the surface area and improve the extraction efficiency;

[0085] Extraction: Select a suitable solvent (such as water, ethanol, methanol, etc.), mix the plant material with the solvent in a certain proportion, extract at an appropriate temperature and time, and use the immersion method for extraction;

[0086] Filtration: Filter the extract to remove solid impurities;

[0087] Concentration: The solvent in the extract is removed by evaporation or other methods to obtain a concentrated plant extract.

[0088] Preferably, the preparation method of PQQ is as follows:

[0089] Using 5-methyl-2,4-dinitroanisole as the starting material:

[0090] The first step: 5-methyl-2,4-dinitroanisole and diethyl oxalate undergo condensation reaction under the action of a base (such as sodium methoxide, sodium ethoxide, sodium isopropoxide, etc.) to obtain ethyl 3-(5-methoxy-2,4-dinitro)phenylpyruvate;

[0091] Step 2: 3-(5-methoxy-2,4-dinitro)phenylpyruvate undergoes a reduction reaction under the action of a reducing agent and then undergoes a ring closure to obtain 6-amino-5-methoxy-1H-indole-2-carboxylic acid ethyl ester; the reducing agent is hydrazine hydrate / RaneyNi, hydrazine hydrate-activated carbon / (FeCl3+AlCl3), 10% Pd-C / H2;

[0092] Step 3: 6-amino-5-methoxy-1H-indole-2-carboxylic acid ethyl ester reacts with glyoxylate and pyruvate in an oxygen atmosphere in a three-component one-pot reaction under the action of a Lewis acid catalyst (such as CuCl2, ZnCl2, etc.) to generate 5-methoxy-1H-pyrrole (2,3-f) quinoline-2,7,9-tricarboxylate;

[0093] Step 4: 5-methoxy-1H-pyrrole (2,3-f) quinoline-2,7,9-tricarboxylate undergoes ester hydrolysis reaction to obtain 5-methoxy-1H-pyrrole (2,3-f) quinoline-2,7,9-tricarboxylic acid;

[0094] Step 5: 5-methoxy-1H-pyrrole (2,3-f) quinoline-2,7,9-tricarboxylic acid undergoes oxidation reaction under the action of oxidant Ce (NH4) 2 (NO3) 6 to obtain PQQ.

[0095] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. An anti-aging composition containing PQQ, comprising PQQ, antioxidants, collagen, hyaluronic acid, and plant extracts, characterized in that: PQQ content is 0.1%-5%; collagen 1%-10%; hyaluronic acid 0.05%-2%; The antioxidants include 0.5%-5% vitamin C, 0.1%-3% α-tocopherol, 0.05%-2% β-carotene, 0.05%-2% coenzyme Q10, and 0.05%-2% glutathione; The plant extracts include grape seed extract with a content of 0.5%-5%, green tea extract with a content of 0.5%-4%, and ginseng extract with a content of 0.1%-3%.

2. An anti-aging composition containing PQQ according to claim 1, characterized in that: The grape seed extract contains proanthocyanidins, catechins, epicatechin polyphenols; the green tea extract is rich in tea polyphenols, including catechins, flavonoids, and anthocyanidins; and the ginseng extract includes a variety of ginsenosides, polysaccharides, and flavonoids.

3. A method for preparing an anti-aging composition containing PQQ, characterized in that: Follow these steps: S1. Raw material preparation: prepare PQQ, antioxidants, collagen, hyaluronic acid, and plant extract raw materials according to the formula ratio; Prepare purified water, ethanol, and acid-base regulator; S2. Dissolution of ingredients: a. Dissolution of PQQ: slowly add PQQ powder into an appropriate amount of solvent. You can first use a small amount of ethanol or other organic solvent to help dissolve it, and then dilute it with pure water to the required concentration; stir evenly to ensure that PQQ is completely dissolved; Pay attention to controlling the dissolution temperature to avoid excessively high temperatures affecting the stability of PQQ; Generally, the dissolution operation can be carried out between room temperature and 40°C; b. Dissolution of antioxidants: Vitamin C can be directly dissolved in pure water. Use 15 ml of water for every gram of vitamin C. Stir evenly at a stirring speed of 200-500 rpm. α-tocopherol, add ethanol according to the ratio of α-tocopherol to ethanol of about 1:10, stir at a speed of 100-300 rpm, and then dilute with purified water; β-carotene, add vegetable oil according to the ratio of β-carotene to vegetable oil of about 1:20, use ultrasound to assist dissolution, the ultrasound power can be set to 200-400W, and the ultrasound time is 20 minutes; Add ethanol at a ratio of about 1:10; stir at a speed of 150-400 rpm for more than 30 minutes; Add glutathione to the solvent at a ratio of about 1:10, stirring at a speed of 200-500 rpm for 10-30 minutes; The room temperature during the above dissolution process is controlled at 20-25°C; c. Dissolution of collagen: Add the collagen powder to an appropriate amount of pure water and stir slowly at a low temperature of 4°C to allow it to gradually dissolve; d. Dissolution of hyaluronic acid: Hyaluronic acid can be directly dissolved in pure water, stirred evenly, and heated to about 40°C to speed up the dissolution; e. Dissolution of plant extracts: Add the solvent in a ratio of grape seed extract to solvent of about 1:10; Green tea extract uses 70-90°C hot water as a solvent, adds hot water in a ratio of about 1:10 of green tea extract to hot water, stirs at a speed of 200-400 rpm, maintains the temperature at 70-90°C, and continues stirring for 15-30 minutes; Add the solvent in a ratio of about 1:10 of ginseng extract to solvent, stir at a speed of 300-500 rpm, stir for 10-20 minutes at room temperature of 20-25°C, then raise the temperature to 40-50°C and continue stirring for 10-20 minutes; S3, mixing: dissolving PQQ and other raw materials in appropriate amounts of solvents respectively, and then mixing the solutions together and stirring evenly; S4. Adjusting pH value: A pH value of the composition can be adjusted using an acid-base regulator so that the pH value can be adjusted between 5 and 7; S5. Filtration: Filter the mixed solution using a filter membrane with a pore size of 0.22 μm to remove impurities and insoluble particles; S6. Filling: Fill the filtered solution into appropriate containers and store them in sealed containers.

4. An anti-aging composition containing PQQ and a preparation method thereof according to claim 3, characterized in that: The plant extract preparation method is as follows: Raw material processing: The plant raw materials are pre-processed by crushing, drying, etc. to increase the surface area and improve the extraction efficiency; Extraction: Select a suitable solvent, mix the plant material and the solvent in a certain proportion, extract at an appropriate temperature and time, and use the immersion method for extraction; Filtration: Filter the extract to remove solid impurities; Concentration: The solvent in the extract is removed by evaporation or other methods to obtain a concentrated plant extract.

5. An anti-aging composition containing PQQ and a preparation method thereof according to claim 4, characterized in that: The preparation method of PQQ is as follows: Using 5-methyl-2,4-dinitroanisole as the starting material: The first step: 5-methyl-2,4-dinitroanisole and diethyl oxalate undergo condensation reaction under the action of a base to prepare ethyl 3-(5-methoxy-2,4-dinitro)phenylpyruvate; Step 2: 3-(5-methoxy-2,4-dinitro)phenylpyruvate undergoes a reduction reaction under the action of a reducing agent and then undergoes a ring closure to obtain 6-amino-5-methoxy-1H-indole-2-carboxylic acid ethyl ester; the reducing agent is hydrazine hydrate / RaneyNi, hydrazine hydrate-activated carbon / (FeCl3+AlCl3), 10% Pd-C / H2; Step 3: 6-amino-5-methoxy-1H-indole-2-carboxylic acid ethyl ester reacts with glyoxylate and pyruvate in an oxygen atmosphere under the action of Lewis acid catalyst to generate 5-methoxy-1H-pyrrole (2,3-f) quinoline-2,7,9-tricarboxylate; Step 4: 5-methoxy-1H-pyrrole (2,3-f) quinoline-2,7,9-tricarboxylate undergoes ester hydrolysis reaction to obtain 5-methoxy-1H-pyrrole (2,3-f) quinoline-2,7,9-tricarboxylic acid; Step 5: 5-methoxy-1H-pyrrole (2,3-f) quinoline-2,7,9-tricarboxylic acid undergoes oxidation reaction under the action of oxidant Ce (NH4) 2 (NO3) 6 to obtain PQQ.

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