Anti-wrinkle and firming composition beneficial to collagen absorption and retention and skin filling

Abstract

The invention relates to the field of anti-wrinkle and firming skin care products, in particular to an anti-wrinkle and firming composition beneficial to collagen absorption and retention and skin filling. An anti-wrinkle firming composition helpful for collagen absorption and retention and filling skin comprises the following components in percentage by mass: 0.05-1% of collagen, 0.1-5% of a perilla frutescens extract, 0.1-5% of a mirabilis jalapa extract, 0.1-5% of a rodendranthus spicatus extract, 0.1-5% of a centella asiatica extract, 0.1-5% of a white truffle fungus extract and a solvent. According to the present invention, the collagen can be easily absorbed, retained and filled into the skin so as to further improve the anti-wrinkle and firming effects.

Description

Technical Field

[0001] The present application relates to the field of anti-wrinkle and firming skin care products, and more particularly, to an anti-wrinkle and firming composition that helps collagen to be absorbed, retained and fill the skin. Background Art

[0002] With the growing demand for beauty and anti-aging, skin firmness and anti-wrinkle properties have become a research hotspot in the field of cosmetics and skin care products. The aging process of the skin is accompanied by a significant loss of collagen, which is one of the main causes of skin sagging, wrinkles and fine lines. Collagen, as the most important structural protein in the skin, is responsible for maintaining the elasticity and firmness of the skin. The decline in its content and quality directly affects the appearance and aging of the skin.

[0003] Collagen plays an important role in the skin. It not only forms the main support of the dermis, but also participates in the adhesion and signal transduction between skin cells. It is essential to maintain the normal physiological function of the skin. However, with the increase of age and the influence of external environmental factors, the synthesis rate of collagen in the skin gradually slows down, while the degradation rate accelerates, resulting in a significant decrease in collagen content. This unbalanced state causes the skin to lose elasticity, become loose and wrinkled, and seriously affects the appearance and touch of the skin.

[0004] Although collagen supplementation is of great significance for improving skin condition, its absorption and retention face many challenges. First, as a large molecular protein, collagen is difficult to directly penetrate the skin barrier and enter the dermis. Secondly, even if some collagen can enter the skin, it is easily degraded by enzymes in the skin, resulting in a limited retention time in the skin. In addition, there are differences in skin conditions and absorption capacity between different individuals, which makes the effect of collagen supplementation vary from person to person, resulting in poor anti-wrinkle and firming effects.

[0005] Therefore, there is room for improvement. Summary of the invention

[0006] In order to solve the above problems and further improve the anti-wrinkle and firming effects, the present application provides an anti-wrinkle and firming composition that helps collagen to be absorbed, retained and fill the skin.

[0007] The anti-wrinkle and firming composition provided by the present application, which helps to absorb, retain and fill the skin with collagen, adopts the following technical solution: An anti-wrinkle and firming composition that helps collagen to be absorbed, retained and filled in the skin, comprising the following components by mass percentage: 0.05-1% collagen, 0.1-5% perilla extract, 0.1-5% Mirabilis jalapa extract, 0.1-5% Cistanche extract, 0.1-5% Centella asiatica extract, 0.1-5% white truffle extract, and a solvent.

[0008] If the plant extracts are not properly selected and formulated, they may not work as expected, and may even have the opposite effect, aggravating skin problems. By adopting the above technical solution and selecting special plant extracts for formulation, safety, effectiveness and stability are ensured.

[0009] Specifically, especially with the cooperation of Perilla extract, Mirabilis jalapa extract and Cistanche deserticola extract, it can quickly penetrate into the skin and effectively promote the production of hyaluronic acid. Hyaluronic acid is a key component for skin moisturizing. It can increase the moisture content of the skin and provide a good living environment for collagen, thereby facilitating the absorption and retention of collagen. Moreover, the cooperation of the three can help to remove some free radicals, delay the cell aging process, and maintain the youthful vitality of the skin. Young skin cells are more likely to absorb and retain collagen.

[0010] The combination of the three can also make the Mirabilis jalapa extract more fully stimulate the synthesis of ceramide and hyaluronic acid, thereby improving the skin barrier function. A healthy skin barrier helps reduce the damage to the skin by external harmful substances, while maintaining the moisture and nutrition of the skin, creating favorable conditions for the absorption and retention of collagen.

[0011] Through various combinations, the overall condition of the skin is further improved, making the skin healthier, smoother and firmer, and easier to absorb and retain collagen.

[0012] Preferably, the anti-wrinkle and firming composition comprises the following components in percentage by mass: 0.1% collagen, 1% perilla extract, 1% Mirabilis jalapa extract, 1% Cistanche deserticola extract, 1% Centella asiatica extract, 1% white truffle extract, and solvent.

[0013] Preferably, the solvent is water.

[0014] By adopting the above technical scheme, the dosage relationship of each component in the anti-wrinkle and firming composition that helps to absorb, retain and fill the skin is further limited, which can give full play to the synergistic effect and promote the absorption and retention of collagen, thereby playing a good anti-wrinkle and firming effect.

[0015] Preferably, the preparation method of the perilla extract comprises the following steps: Step a: washing and chopping the perilla, and mixing it with water to obtain a perilla solution, wherein the mass ratio of the perilla to the water is 1:(3-5); Step b: subjecting the perilla solution to microwave treatment to obtain a microwave perilla solution; Step c: mixing the microwave perilla solution with Propionibacterium freudenreichii and Rhodotorula glutinosae, and culturing them at 25-30° C. for 40-50 hours to obtain a perilla mixed solution; the mass ratio of Propionibacterium freudenreichii, Rhodotorula glutinosae to the microwave perilla solution is (0.001-0.005):(0.001-0.005):1, with the mass of the microwave perilla solution as the benchmark; Step d: centrifuging the perilla mixed solution, taking the supernatant and filtering it to obtain the perilla extract.

[0016] Perilla extract itself does not have a cooling effect, but in actual research, it was found that the perilla extract obtained by the above technical solution, when combined with Mirabilis jalapa extract and Cistanche deserticola extract, can effectively relieve skin discomfort. Especially when the skin is sensitive, it can reduce the user's discomfort, relieve redness and swelling, give the user a cooling and refreshing feeling, improve skin conditions, and make the skin absorb collagen faster and better, so as to achieve a more significant anti-wrinkle and firming effect.

[0017] Preferably, the mass ratio of perilla to water is 1:5, and the mass ratio of Propionibacterium freudenreichii, Rhodotorula glutinosae and microwave perilla solution is 0.002:0.002:1.

[0018] By adopting the above technical solution, the dosage relationship between Propionibacterium freudenreichii, Rhodotorula glutinosae and the microwave perilla solution is further limited. In the mixed preparation, more effective ingredients can be better released, and the active ingredients in the extract are changed through the metabolic activities of Propionibacterium freudenreichii and Rhodotorula glutinosae, thereby obtaining a specific perilla extract with better performance.

[0019] Preferably, the microwave treatment in step b is carried out at 65-75° C. and 300-400 W for 3-5 min.

[0020] By adopting the above technical solution and further limiting the conditions of microwave treatment, the entire system can be rapidly heated and evenly heated, which helps to release more active substances and is also beneficial for the subsequent cooperation with Propionibacterium freudenreichii and Rhodotorula mucilaginosa.

[0021] Preferably, the method for preparing the anti-wrinkle and firming combination that helps collagen to be absorbed, retained and filled in the skin is to mix all the raw materials until they are uniform.

[0022] Preferably, all raw materials are mixed under stirring at 1000-1500 r / min.

[0023] By adopting the above technical scheme, the mixing conditions of each raw material are further limited, and the mixing is carried out at a higher speed, so that the raw materials are mixed more fully and evenly. After contacting with the skin, they can respond quickly and penetrate into the skin to enhance the skin's absorption capacity for collagen, thereby playing a better anti-wrinkle and firming effect.

[0024] In summary, this application has the following beneficial effects: 1. With the cooperation of Perilla extract, Mirabilis jalapa extract and Cistanche deserticola extract, it improves the overall condition of the skin from multiple aspects such as promoting the production of hyaluronic acid and stimulating the synthesis of ceramides, creating favorable conditions for the absorption and retention of collagen, making the skin healthier, smoother and firmer, and easier to absorb and retain collagen.

[0025] 2. In this application, the perilla extract is preferably prepared in a specific manner, and after being combined with the Mirabilis jalapa extract and the Cistanche deserticola extract, it can effectively relieve skin discomfort. Especially when the skin is sensitive, it can reduce the user's discomfort, relieve redness and swelling, give the user a feeling similar to cooling and cooling, improve skin conditions, and make the skin absorb collagen faster and better, so as to play a more significant anti-wrinkle and firming effect. DETAILED DESCRIPTION

[0026] The present application is further described in detail below in conjunction with embodiments.

[0027] The raw materials used in the following examples and comparative examples are all commercially available products. Example

[0028] Example 1 An anti-wrinkle and firming composition that helps collagen to be absorbed, retained and filled in the skin comprises the following components: collagen, perilla extract, Mirabilis jalapa extract, Cistanche deserticola extract, Centella asiatica extract, white truffle extract and a solvent.

[0029] The content of each component is shown in Table 1.

[0030] Collagen was commercially available from Shaanxi Jinbo Biopharmaceutical Co., Ltd., model number is micoreCol.III-CP5-SMART.

[0031] The perilla extract is homemade, and the preparation method comprises the following steps: Step a: Wash the perilla with clean water and then chop it into small pieces. Mix the chopped perilla with water in a mass ratio of 1:4 to obtain a perilla solution.

[0032] Step b: microwave the perilla solution at 70° C. and 350 W for 4 min to obtain a microwave perilla solution.

[0033] Step c: based on the mass of the microwave perilla solution, the mass ratio of Propionibacterium freudenreichii, Rhodotorula glutinosus to the microwave perilla solution is 0.002:0.002:1, and Propionibacterium freudenreichii and Rhodotorula glutinosus are prepared.

[0034] Add Propionibacterium freudenreichii and Rhodotorula glutinosae into the microwave perilla solution and mix until uniform.

[0035] The mixture was cultured at 28°C for 48 hours to obtain a perilla mixed solution.

[0036] Step d: centrifuge the perilla mixed solution, take the supernatant, and filter it with a 0.3 μm filter membrane to obtain a perilla extract.

[0037] Propionibacterium freudenreichii was purchased from Hubei Haijia Biotechnology Co., Ltd.

[0038] Rhodotorula glutinosae was purchased from Ruichu Biotechnology (Jiangsu) Co., Ltd.

[0039] Mirabilis jalapa extract was commercially available from Lanzhou Waterless Biotechnology Co., Ltd.

[0040] Yellow flower nine wheel grass extract is commercially available from Xi'an Four Leaf Clover Biotechnology Co., Ltd. Centella asiatica extract is commercially available and purchased from Xi'an Ruierli Bioengineering Co., Ltd. White truffle extract is commercially available and purchased from Shaanxi Dongjiang Kangtai Health Industry Co., Ltd.

[0041] The solvent is water.

[0042] The method for preparing an anti-wrinkle and firming composition that helps collagen to be absorbed, retained and filled in the skin comprises the following steps: all raw materials are mixed uniformly at a stirring speed of 1200 r / min to obtain an anti-wrinkle and firming composition that helps collagen to be absorbed, retained and filled in the skin.

[0043] Example 2 An anti-wrinkle and firming composition that helps collagen to be absorbed, retained and fill the skin, which is different from Example 1 in that it includes the following components: collagen, perilla extract, Mirabilis jalapa extract, Cistanche deserticola extract, Centella asiatica extract, white truffle extract, and solvent.

[0044] The content of each component is shown in Table 1.

[0045] Collagen was commercially available from Shaanxi Jinbo Biopharmaceutical Co., Ltd., model number is micoreCol.III-CP5-SMART.

[0046] The perilla extract is homemade, and the preparation method comprises the following steps: Step a: Wash the perilla with clean water and then chop it into small pieces. Mix the chopped perilla with water in a mass ratio of 1:5 to obtain a perilla solution.

[0047] Step b: microwave the perilla solution at 65° C. and 400 W for 3 min to obtain a microwave perilla solution.

[0048] Step c: based on the mass of the microwave perilla solution, the mass ratio of Propionibacterium freudenreichii, Rhodotorula glutinosus to the microwave perilla solution is 0.005:0.005:1, and Propionibacterium freudenreichii and Rhodotorula glutinosus are prepared.

[0049] Add Propionibacterium freudenreichii and Rhodotorula glutinosae into the microwave perilla solution and mix until uniform.

[0050] The mixture was cultured at 25°C for 50 hours to obtain a perilla mixed solution.

[0051] Step d: centrifuge the perilla mixed solution, take the supernatant, and filter it with a 0.3 μm filter membrane to obtain a perilla extract.

[0052] Propionibacterium freudenreichii was purchased from Hubei Haijia Biotechnology Co., Ltd.

[0053] Rhodotorula glutinosae was purchased from Ruichu Biotechnology (Jiangsu) Co., Ltd.

[0054] Mirabilis jalapa extract was commercially available from Lanzhou Waterless Biotechnology Co., Ltd.

[0055] Yellow flower nine wheel grass extract is commercially available from Xi'an Four Leaf Clover Biotechnology Co., Ltd. Centella asiatica extract is commercially available and purchased from Xi'an Ruierli Bioengineering Co., Ltd. White truffle extract is commercially available and purchased from Shaanxi Dongjiang Kangtai Health Industry Co., Ltd.

[0056] The solvent is water.

[0057] The method for preparing an anti-wrinkle and firming composition that helps collagen to be absorbed, retained and filled in the skin comprises the following steps: all raw materials are mixed uniformly at a stirring speed of 1000 r / min to obtain an anti-wrinkle and firming composition that helps collagen to be absorbed, retained and filled in the skin.

[0058] Example 3 An anti-wrinkle and firming composition that helps collagen to be absorbed, retained and fill the skin, which is different from Example 1 in that it includes the following components: collagen, perilla extract, Mirabilis jalapa extract, Cistanche deserticola extract, Centella asiatica extract, white truffle extract, and solvent.

[0059] The content of each component is shown in Table 1.

[0060] Collagen was commercially available from Shaanxi Jinbo Biopharmaceutical Co., Ltd., model number is micoreCol.III-CP5-SMART.

[0061] The perilla extract is homemade, and the preparation method comprises the following steps: Step a: Wash the perilla with clean water and then chop it into small pieces. Mix the chopped perilla with water in a mass ratio of 1:3 to obtain a perilla solution.

[0062] Step b: microwave the perilla solution at 75° C. and 300 W for 5 min to obtain a microwave perilla solution.

[0063] Step c: based on the mass of the microwave perilla solution, the mass ratio of Propionibacterium freudenreichii, Rhodotorula glutinosus to the microwave perilla solution is 0.001:0.001:1, and Propionibacterium freudenreichii and Rhodotorula glutinosus are prepared.

[0064] Add Propionibacterium freudenreichii and Rhodotorula glutinosae into the microwave perilla solution and mix until uniform.

[0065] The mixture was cultured at 30°C for 40 hours to obtain a perilla mixed solution.

[0066] Step d: centrifuge the perilla mixed solution, take the supernatant, and filter it with a 0.3 μm filter membrane to obtain a perilla extract.

[0067] Propionibacterium freudenreichii was purchased from Hubei Haijia Biotechnology Co., Ltd.

[0068] Rhodotorula glutinosae was purchased from Ruichu Biotechnology (Jiangsu) Co., Ltd.

[0069] Mirabilis jalapa extract was commercially available from Lanzhou Waterless Biotechnology Co., Ltd.

[0070] Yellow flower nine wheel grass extract is commercially available from Xi'an Four Leaf Clover Biotechnology Co., Ltd. Centella asiatica extract is commercially available and purchased from Xi'an Ruierli Bioengineering Co., Ltd. White truffle extract is commercially available and purchased from Shaanxi Dongjiang Kangtai Health Industry Co., Ltd.

[0071] The solvent is water.

[0072] The method for preparing an anti-wrinkle and firming composition that helps collagen to be absorbed, retained and filled in the skin comprises the following steps: all raw materials are mixed uniformly at a stirring speed of 1500 r / min to obtain an anti-wrinkle and firming composition that helps collagen to be absorbed, retained and filled in the skin.

[0073] Table 1 Category Example 1 Example 2 Example 3 collagen(%) 0.1 0.05 1 Perilla extract (%) 1 0.1 5 Mirabilis jalapa extract (%) 1 0.1 5 Cistanche deserticola extract (%) 1 0.1 5 Centella asiatica extract (%) 1 0.1 5 White Truffle Extract (%) 1 0.1 5 Solvent (%) to 100 to 100 to 100 Comparative Example Comparative Example 1 An anti-wrinkle and firming composition that helps collagen to be absorbed, retained and filled in the skin, which is different from Example 1 in that white truffle extract is used instead of perilla extract.

[0074] Comparative Example 2 An anti-wrinkle and firming composition that helps collagen to be absorbed, retained and fill the skin, which is different from Example 1 in that white truffle extract is used to replace Mirabilis jalapa extract.

[0075] Comparative Example 3 An anti-wrinkle and firming composition that helps collagen to be absorbed, retained and fill the skin, which is different from Example 1 in that white truffle extract is used to replace Cinnamon bark extract.

[0076] Comparative Example 4 An anti-wrinkle and firming composition that helps collagen to be absorbed, retained and filled in the skin, which is different from Example 1 in that the preparation method of the perilla extract comprises the following steps: Step a: Wash the perilla with clean water and then chop it into small pieces. Mix the chopped perilla with water in a mass ratio of 1:4 to obtain a perilla solution.

[0077] Step b: microwave the perilla solution at 70° C. and 350 W for 4 min to obtain a microwave perilla solution.

[0078] Step c: based on the mass of the microwave perilla solution, the mass ratio of red yeast to the microwave perilla solution is 0.004:1, and red yeast is prepared.

[0079] Add red yeast to the microwave perilla solution and mix until uniform.

[0080] The mixture was cultured at 28°C for 48 hours to obtain a perilla mixed solution.

[0081] Step d: centrifuge the perilla mixed solution, take the supernatant, and filter it with a 0.3 μm filter membrane to obtain a perilla extract.

[0082] Comparative Example 5 An anti-wrinkle and firming composition that helps collagen to be absorbed, retained and filled in the skin, which is different from Example 1 in that the preparation method of the perilla extract comprises the following steps: Step a: Wash the perilla with clean water and then chop it into small pieces. Mix the chopped perilla with water in a mass ratio of 1:4 to obtain a perilla solution.

[0083] Step b: microwave the perilla solution at 70° C. and 350 W for 4 min to obtain a microwave perilla solution.

[0084] Step c: Based on the mass of the microwave perilla solution, the mass ratio of Propionibacterium freudenreichii to the microwave perilla solution is 0.004:1, and Propionibacterium freudenreichii is prepared.

[0085] Add Propionibacterium freudenreichii into the microwave perilla solution and mix until uniform.

[0086] The mixture was cultured at 28°C for 48 hours to obtain a perilla mixed solution.

[0087] Step d: centrifuge the perilla mixed solution, take the supernatant, and filter it with a 0.3 μm filter membrane to obtain a perilla extract.

[0088] Performance testing Test sample preparation: A serum comprises the following components in percentage by mass: 5% 1,3-propylene glycol, 2% polyglycerol-10, 1% dipropylene glycol, 1% propylene glycol, 0.5% ammonium acryloyldimethyltaurate / VP copolymer, 0.1% phenoxyethanol, 0.2% ethylhexylglycerin, 0.2% sodium hyaluronate, 0.06% carbomer, 0.06% triethanolamine, 1% anti-wrinkle and firming composition that helps collagen to be absorbed, retained and filled in the skin, and the remainder is water.

[0089] Mix the above components until uniform to obtain essence.

[0090] Volunteer Selection: 80 female volunteers aged 35-40 were selected. They had eye wrinkles and the degree of wrinkles was similar among the volunteers. They were randomly divided into 8 groups, each with 10 people, and each group corresponded to a test sample.

[0091] 1. Anti-wrinkle: Volunteers clean their faces with clean water, take 2 mL of the test sample and apply it evenly on the face, once in the morning and once in the evening.

[0092] Before the test and 28 days after the test sample was used, the face was photographed with a facial image analyzer VISIA to record the wrinkle area and wrinkle depth.

[0093] The wrinkle area change rate and wrinkle depth change rate were calculated according to the following formula, and the average value of the volunteers in the same group was used as the group data, which was recorded in Table 2.

[0094] 2. Firming: Volunteers cleanse their faces with clean water, take 2mL of the test sample and apply it evenly on the face, once in the morning and once in the evening.

[0095] Before the test and 28 days after the test sample was used, the skin elasticity tester tested the skin elasticity and recorded the R2 value.

[0096] The skin elasticity change rate was calculated according to the following formula, and the average value of the volunteers in the same group was used as the group data, which is recorded in Table 2.

[0097] Table 2 According to the results in Table 2, compared with Comparative Examples 1-5, the test samples of Examples 1-3 showed better anti-wrinkle and firming effects, indicating that the combination of Perilla extract, Mirabilis jalapa extract, and Cistanche deserticola extract can make the skin better absorb and retain collagen, fill the skin, and achieve a firming and anti-wrinkle effect.

[0098] 3. Sensitive skin test: 80 female volunteers aged 35-40 who self-diagnosed as having sensitive skin were selected and randomly divided into 8 groups, with 10 people in each group, and each group corresponds to one test sample.

[0099] Wash your face with water, then apply 2 drops of 2% histamine solution on your face. When the skin becomes obviously red, swollen or itchy, apply 2 mL of the test sample evenly to the irritated area.

[0100] Questionnaires were distributed to the volunteers to ask about their feelings after using the test samples. The questionnaire included the following contents: (1) Whether there is a cooling or refreshing feeling. The score is 0-10, and the closer to 10, the stronger the cooling or refreshing feeling. The average score of the volunteers in the same group is recorded in Table 3.

[0101] 0-4 points represent that the cooling or refreshing feeling is not strong or weak, 5-7 points represent that the cooling or refreshing feeling is more obvious, and 8-10 points represent that the cooling or refreshing feeling is strong.

[0102] (2) Test whether the sample causes discomfort. Use "yes" or "no" to represent the result, and record the number of people who "yes" feel discomfort in Table 3.

[0103] (3) Whether it can relieve discomfort such as redness, swelling, itching, etc. The number of people who chose “can effectively relieve”, “can relieve, but the effect is average”, and “cannot relieve” were recorded in Table 3.

[0104] Table 3 According to the test results in Table 3, the test samples of Examples 1-3 can give volunteers a feeling similar to cooling and refreshing, and can effectively relieve discomfort. The test samples of Comparative Examples 1-5 are not as effective in relieving discomfort as Examples 1-3.

[0105] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. An anti-wrinkle and firming composition that helps collagen to be absorbed, retained and filled in the skin, characterized in that: The invention comprises the following components in percentage by mass: 0.05-1% collagen, 0.1-5% perilla extract, 0.1-5% mirabilis jalapa extract, 0.1-5% cleome extract, 0.1-5% centella asiatica extract, 0.1-5% white truffle extract and solvent.

2. The anti-wrinkle and firming composition according to claim 1 that helps collagen to be absorbed, retained and filled in the skin, characterized in that: The anti-wrinkle and firming composition comprises the following components in terms of mass percentage: 0.1% collagen, 1% perilla extract, 1% Mirabilis jalapa extract, 1% Cistanche deserticola extract, 1% Centella asiatica extract, 1% white truffle extract, and solvent.

3. The anti-wrinkle and firming composition according to claim 1 that helps collagen to be absorbed, retained and filled in the skin, characterized in that: The solvent is water.

4. The anti-wrinkle and firming composition according to claim 1 that helps collagen to be absorbed, retained and filled in the skin, characterized in that: The preparation method of the perilla extract comprises the following steps: Step a: washing and chopping the perilla, and mixing it with water to obtain a perilla solution, wherein the mass ratio of the perilla to the water is 1:(3-5); Step b: subjecting the perilla solution to microwave treatment to obtain a microwave perilla solution; Step c: mixing the microwave perilla solution with Propionibacterium freudenreichii and Rhodotorula glutinosae, and culturing them at 25-30° C. for 40-50 hours to obtain a perilla mixed solution; the mass ratio of Propionibacterium freudenreichii, Rhodotorula glutinosae to the microwave perilla solution is (0.001-0.005): (0.001-0.005): 1, with the mass of the microwave perilla solution as the reference; Step d: centrifuging the perilla mixed solution, taking the supernatant and filtering it to obtain the perilla extract.

5. The anti-wrinkle and firming composition according to claim 4 that helps collagen to be absorbed, retained and filled in the skin, characterized in that: The mass ratio of perilla to water is 1:5, and the mass ratio of Propionibacterium freudenreichii, red yeast and microwave perilla solution is 0.002:0.002:

1.

6. The anti-wrinkle and firming composition according to claim 4 that helps collagen to be absorbed, retained and filled in the skin, characterized in that: The microwave treatment in step b is carried out at 65-75° C. and 300-400 W for 3-5 min.

7. The anti-wrinkle and firming composition that helps collagen absorption, retention and filling of the skin according to any one of claims 1 to 6, characterized in that: The preparation method of the anti-wrinkle and firming combination that helps collagen absorption, retention and filling of the skin is to mix all the raw materials until they are uniform.

8. The anti-wrinkle and firming composition according to claim 7 that helps collagen to be absorbed, retained and filled in the skin, characterized in that: When mixing all the raw materials, stir at 1000-1500r / min.

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