The invention belongs to the technical field of
fermentation engineering, and particularly discloses a low-cost
fermentation medium for
bifidobacterium lactis. At present, most
bifidobacterium lactis culture media adopt MRS culture media,
nitrogen sources of the MRS culture media comprise
beef extract powder,
yeast extract
powder, peptone and the like, and the MRS culture media based on the
nitrogen sources are high in price and high in large-scale industrial cost. In the microbial
fermentation industry, the cost of the fermentation culture medium is about one third of the fermentation production cost, the main cost of the MRS culture medium is the
nitrogen source, and the fermentation production cost can be reduced by reducing the cost of the
nitrogen source. The
corn steep liquor dry powder is a
plant nitrogen source suitable for a
bifidobacterium culture medium and contains rich soluble
protein,
amino acid, vitamins, inorganic salt and the like, the
protein content is about 40%, and the nutritional cost of the
corn steep liquor dry powder meets the breeding requirement of
bifidobacterium lactis. The
corn steep liquor dry powder is low in price, the purchase price of the corn steep liquor dry
powder is only one tenth or lower than that of
beef extract powder and peptone, and the large-scale industrial production cost can be reduced. The formula of the culture medium comprises 10-20 g / L of
galactooligosaccharide, 5-10 g / L of
glucose monohydrate, 30-40 g / L of corn steep liquor dry powder, 5-10 g / L of
yeast peptone and 1-3 g / L of
anhydrous sodium acetate. According to the invention, the water-soluble component of the dry powder of the corn steep liquor is used as a main
nitrogen source of the culture medium, and the purposes of sustainable utilization of resources and yield improvement in the production of
bifidobacterium lactis can be achieved through the application of the culture medium.