The invention relates to a preparation method of a traditional Chinese medicine for treating keratitis caused by fire excess from yin deficiency, which belongs to the traditional Chinese medicine preparation method technical field. Currently, the treatment for treating liver-fire excess type keratitis generally employs sulfonamides and antibiotics, and the disadvantage of sulfamethoxazole can lead to gastrointestinal tract reaction, crystalluria, hematuria, fash, drug fever and leucopenia. A technical scheme is characterized in that radix rehmanniae recen, radix scrophulariae, ophiopogon root, radix asparagi, Dendrobium, radix polygonati officinalis, lily, tortoiseshell, Salvia miltiorrhiza, prepared rehmannia root, mulberry fruit, prunella spike, semen celosiae, butterflybush flower, pipewort, scouring rush, chrysanthemum, mint, cicada slough, silkworm larva, Trillium tschonoskii Maxim, semen cassiae, rauvolfia verticillate, fructu broussonetiae, Faeces Vespertilionis, Polygonum orientale and licorice are taken, the above 27 traditional Chinese medicines are placed in water for immersing, and then decocted by slow fire, filtered to remove the slag, and the decocted soup is the traditional Chinese medicine for treating keratitis caused by fire excess from yin deficiency. The prepared traditional Chinese medicine has the advantages of small toxic and side effects, short treatment course and high cure rate. The adverse reaction generated by western medicine can be avoided.