149results about How to "Simple separation and purification process" patented technology

The invention relates to a rhodococcus ruber and a method for preparing 5-cyanovaleramide by utilizing the same. The strain needs oxygen, does not need power, spores and gram-positive bacteria, is already stored in the Common Microorganism Center of Chinese Microorganism Strain Storage Management Committee and has storage data of Jun. 4, 2009 and a preservation number of CGMCC No.3090. The method for preparing the 5-cyanovaleramide by utilizing the rhodococcus ruber comprises the following steps: (1) culturing seeds; (2) fermenting and culturing; (3) preparing a rhodococcus ruber suspension; (4) preparing a crude product of 5-cyanovaleramide; and (5) purifying the 5-cyanovaleramide. The invention provides a method for generating the 5-cyanovaleramide by selectively hydrolyzing adiponitrile in a rhodococcus ruber region, has high catalytic efficiency, high adiponitrile conversion rate up to 100 percent and 5-cyanovaleramide regioselectivity up to 100 percent, does not generate a side product of adipamide and simplifies the separation purification process of the 5-cyanovaleramide.

The invention relates to a method for synthesizing salidroside by utilizing enzyme catalyzed direct glucosylation, comprising the following steps: carrying out a reaction on glucose, tyrosol and enzyme in a solvent containing a buffer solution; and collecting the salidroside by adopting the conventional method, wherein, the solvent comprises an icon-containing liquid and an organic solvent. Compared with the existing method, in the method, a reaction medium composed of the ionic liquid is adopted as a reaction system for synthesizing the salidroside by utilizing the enzyme catalyzed direct glucosylation; the enzyme activity and the enzyme stability are ensured; the dissolubility of the substrate in the reaction system is improved; the water activity in the reaction system is reduced; the expensive glucoside donor needs not to be used, and the salidroside can be further synthesized; the separation and purification processes of the product are simple, the unreacted tyrosol can be recovered and recycled, thus lowering the production cost, and satisfying the requirements of rapidly developing the medical industries.

The invention provides a preparation process of nano carbon crystals and belongs to the field of carbon material preparation. The preparation process includes the following steps of acid washing, wherein diamond raw materials are crushed into fine powder being over 10000 meshes in particle size, and after the fine powder is subjected to acid washing and extraction, the fine powder is washed with deionized water till the pH of cleaning fluid is close to 7; screening, wherein the cleaned materials are subjected to centrifugal separation, supernatant liquid is picked for precipitation separation for 4-7 days, the supernatant liquor is removed, and a finished product of the nano carbon crystals is obtained after lower sediment is dried. The preparation process is low in lost, simple in equipment, simple in operation and high in safety of operating staff, simple in separation and extraction process and little in pollution to the environment.

The invention relates to a preparation method of 2,6-difluorobenzamide by utilizing rhodococcus ruber. The method comprises the following steps: (1) fermentation and culture of seeds; (2) preparation of rhodococcus ruber suspension fluid; (3) conversion of 2,6-difluorobenzonitrile; (4) preparation of finished products. The invention provides a preparation method of 2,6-difluorobenzamide by utilizing rhodococcus ruber to convert 2,6-difluorobenzonitrile into 2,6-difluorobenzamide. The microorganism conversion method has a very high catalytic efficiency, increases the substrate conversion rate, under a material concentration of 3.5 mol/L, the 2,6-difluorobentrinile conversion rate is as high as 100%, the 2,6-difluorobenamide selectivity is 100%, and no by product 2,6- difluorobenzoic acid is generated. The separation and purification technology of target product is simplified, and the method has the advantages of mild reaction conditions, low requirements on equipment, and easy application to production.

The invention discloses functional mulberry leaf oligosaccharide and a preparation method and application thereof. The method comprises the following steps that 1, dried mulberry leaves serve as raw materials, after crushing is conducted, degreasing is conducted by means of petroleum ether, and degreased mulberry leaf powder is obtained; 2, a mulberry leaf polysaccharide extracting solution is obtained by conducting water extraction on the degreased mulberry leaf powder; 3, hydrolase is added in the obtained mulberry leaf polysaccharide extracting solution, and oligosaccharide hydrolysate is obtained through enzymolysis; 4, ethanol is added in the oligosaccharide hydrolysate, unreacted mulberry leaf polysaccharide is subjected to alcohol precipitation, and a mulberry leaf oligosaccharide solution with polysaccharide removed is obtained; 5, saccharomyces cerevisiae is added in the mulberry leaf oligosaccharide solution with the polysaccharide removed, fermentation is conducted to removemonosaccharide, and finally the functional mulberry leaf oligosaccharide is obtained. Accordingly, alcohol precipitation is utilized for removing the polysaccharide, microbial fermentation is utilized for removing the monosaccharide, the mulberry leaf oligosaccharide separation and purification process is simplified, the functional mulberry leaf oligosaccharide has better economical efficiency and environmental friendliness, and the obtained mulberry leaf oligosaccharide has the physiological efficacy of promoting proliferation of intestinal beneficial bacteria and inhibiting growth of harmful bacteria.

The invention relates to recombinant strain capable of expressing fungal immunomodulatory protein from Flammulina velutipes (FIP-fve), a construction method, a protein expression and purification method, and protein application. Existing FIP-fve engineered strains have the disadvantages of low expression rate, expression product of insoluble inclusion body, uncertain structure and broken strain, complex purification process, and non-suitability for large-scale production. The invention adopts FIP-fve gene and Transetta (DE3) as host bacteria to construct recombinant strain; induces expression with isopropyl-beta-d-thiogalactoside (IPTG); treats by combining a lysozyme method and ultrasonic method; and desalts by adopting Ni-His.Band column chromatography and Sephadex G25 gel column chromatography, thereby finishing construction of recombinant strain, expression and purification. The invention is used for preparing drug for preventing or treating allergy. The purification process is simple and rapid, and suitable for large-scale fermentation production.

The invention discloses a method for efficiently separating immunocompetence polysaccharide of ganoderma atrum. Refined ganoderma atrum polysaccharide PSG serves as raw materials of the method, strong anion exchange resin Q-Sepharose Fast Flow is adopted for separating and purifying the PSG, sodium chloride solutions in different concentrations (0-2 M) are used for performing stepwise elution, different obtained components are sequentially dialyzed, frozen and dried, and ganoderma atrume acidic beta-(1->3, 1->6)-glucan polysaccharide components with immunocompetence are obtained. The method has the advantages that different chemical components in the PSG can be effectively separated by the strong anion exchange resin; the prepared acidic polysaccharide is high in sugar content, good in uniformity and superior to refined polysaccharide PSG in immunocompetence; the method is easy to operate, fast, efficient and good in reproducibility, elution liquid in use is free of toxin and pollution, and the method can be widely used for large-scale preparation of immunocompetence polysaccharide of ganoderma atrum.

The invention relates to a preparation method for synthesizing alpha-arbutin through immobilizing hydroquinone by a macro-porous adsorption resin and carrying out fermentation catalysis of the immobilized hydroquinone and disaccharide by Xanthomonas campestris having a preservation number of CGMCC NO.1243. The method comprises the steps of immobilized hydroquinone preparation, microbial fermentation, reaction condition optimization, and product separation and extraction. The alpha-arbutin output of the method is 20-235g each liter of a broth, and is 14 times higher than the output of alpha-arbutin obtained by directly using hydroquinone as a reactant, the hydroquinone conversion rate of the method reaches 90.5-95.2%, and the purity of the above obtained separated and purified product can reach above 99.5%. The immobilization of the hydroquinone improves the tolerance of a thallus to hydroquinone, simplifies the subsequent separating and purifying technology of the product, and reduces the phenol-containing wastewater emission of the above system; and the method has the advantages of high output, simple production technology, low production cost, environmental protection, and substantial reduction of the alpha-arbutin production cost, and lays a foundation for the large-scale low-cost production of alpha-arbutin.

The invention provides a human keratinocyte growth factor -1 structure analogues KGF-1delta 23KGF(40S), an N end of an amino acid sequence of which lacks 23 amino acids, while the 40-bit cysteine point of which is mutated into a nonpolar amino acid. The invention also relates to a production method of the structure analogues, which carries out the fusion expression with a small ubiquitin related modifier gene mature peptide, while a fusion protein and the ubiquitin related modifier gene protease 1 co-express in the prokaryotes. In the process of fermentation expression, the ubiquitin related modifier gene protease 1 can hydrolyze the fusion protein to produce a soluble KGF-1delta 23KGF (40S). The human keratinocyte growth factor structure analogues can facilitate the proliferation of the keratinocyte cells, the growth of the hair follicle cells and inhibit the growth of the fibroblast cells, and has the functions of anti-scar, anti-fibrosis, epidermis healing facilitation and corneal wound reparation, etc.

The disclosed preparation method for beta-cyclodextrin comprises: with gene engineering technique, fixing the cyclodextrin glycosyl transfer enzyme expression on surface of saccharomyces cerevisiae cell to ferment and prepare the product; centrifugal removing just the thali cell and residue for separation and purification; condensing the supernatant to obtain the final product. This invention keeps enzyme activity for reutilization, improves yield by producing ethanol and consuming the glucose by yeast, and has wide application with unassailable security.

The invention discloses a preparation method of biobased pentaerythritol fatty acid ester, which belongs to the technical field of esterification and synthesis in organic chemistry. Specifically, pentaerythritol is mixed with biobased fatty acid, flowing degassing is performed under a negative pressure condition for an autocatalytic esterification reaction, and refined processes of separation dealcoholization, distillation deacidification and the like are performed, so as to obtain biobased pentaerythritol fatty acid ester. The preparation method has the advantages that a catalyst does not need to be added in the autocatalytic esterification reaction, thereby preventing a residual catalyst existing in a combined-state form in product molecules in a synthesis process of the traditional catalytic esterification technology commonly adopted at present from affecting the overall performance of a product; separation and purification processes are simple, and are controlled easily; the degree of esterification is high, side reaction is less, and the product purity is high; the high-temperature stability of the product is good.

The invention relates to a method for preparing carbamate. The method for preparing the carbamate comprises the following processes of: dissolving carbonic ester, amine and acetate catalyst into a proper solvent so that the molar ratio of the carbonic diester to the amine is 2 to 20 times stoichiometric ratio and the molar ratio of the catalyst to the amine is 1:5-1:100; and then reacting for 0.5 to 16 hours at the temperature of between 30 and 160 DEG C, and performing separation and purification to obtain the required carbamate. The method has the main advantages that: the catalyst has stable chemical performance and low price; and the product separation and purification processes are simple.

The invention discloses a method for extracting and separating proanthocyanidins from lycium ruthenicum murr. According to the method, a high-speed shearing wall-breaking technology, a macroporous resin mixed simulated moving bed enrichment technology and a nanofiltration membrane separation and purification technology are adopted as core technologies, lycium ruthenicum murr with the highest content of proanthocyanidins is used as a raw material, and green solvents water and ethanol are used as solvents to develop a proanthocyanidins industrialization technology and its products in line with the international mainstream product specifications. The content of proanthocyanidins in the obtained product is greater than or equal to 85%, OPC: 65%-80%, and polyphenol content is greater than or equal to 85%. The process not only helps improve the purity and yield of oligoprocyanidins, but also meets the requirements of industrial production on raw materials, solvent use, production route, production process safety, product color, yield and purity. The process is a pure green production process. by the process, there is organic residues, output is high, and product quality can reach the standards of mainstream products in the international market. The product can be used as a raw material for food, health food and special medical uses.

This invention discloses an earthworm plasminogen gene and gene engineering strain and its construction and its application, in which, the plasminogen gene is composed of 747 nuleotide, coding 246 amino acid, which gene expresses active gene engineering plasminogen protein, the fermented broth active unit surpasses 3, 390, 000U/L.

The invention discloses a rapid joint analysis method for Pu-239, Sr-90 and Cs-137 in waste liquid. The rapid joint analysis method comprises the following steps of: precipitating plutonium and strontium by controlling a pH value of wastewater, and separating then from cesium; dissolving plutonium and strontium precipitates, meanwhile, adjusting a valence state of Pu to Pu(IV), separating 239Pu and 90Sr through using a TEVA resin and Sr resin double-column series connection method, and measuring 239Pu and 90Sr through using ICP-MS and a liquid scintillation counter respectively; and collectingall liquid in the plutonium and strontium precipitation process, directly enriching 137Cs through using KNiF-PAN resin, naturally airing the resin, and measuring 137Cs by using a gamma spectrometer.The rapid joint analysis method has the advantages of small sample size, simple process, short experiment period, high separation efficiency and the like, and a feasible and efficient technical meanscan be provided for rapid analysis of nuclide in nuclear emergency monitoring and nuclear safety supervision.

The invention provides a method for improving the quality of biodiesel. The method comprises the following steps: 1, reacting oil, short-chain alcohol, water and liquid lipase in a one-stage or multi-stage enzyme reactor, separating a reaction solution into a heavy phase and a light phase, and recycling enzymes in the heavy phase, the light phase being used for subsequent immobilized enzyme transformation; 2, causing the light phase obtained in step 1 to flow into a one-stage or multi-stage reactor with immobilized lipase, adding short-chain alcohol for reaction, and performing online dehydration in a reaction process; 3, causing a reaction solution obtained in step 2 to flow into an enzyme reactor of the next stage to be reacted with dimethyl carbonate or diethyl carbonate, and performing online hydration in a reaction process to further implement complete transformation of the oil to biodiesel, wherein the yield of the biodiesel exceeds 98 percent, and the total glycerin content is lower than 0.2 percent. According to the method, low-quality non-edible oil can be transformed into high-quality biodiesel, a subsequent separation and purification process is greatly simplified, and good economic benefits and social benefits are achieved.

The invention discloses saccharifying enzyme high-yield strain gene knockout recombinant bacteria with low trans-glycoside enzyme background as well as a construction method and application thereof and belongs to the technical field of biology. The recombinant bacteria are obtained by inactivating one or more genes relevant to alpha-glucose trans-glycoside enzyme expression. Experiments prove that a new strain with low trans-glycoside enzyme activity is obtained by performing gene engineering reconstruction on aspergillus niger pyrG defect-type Aspergillus niger SH-2:delta pyrG with high saccharifying enzyme yield, and inactivating genes agdB, agdA and agdE. Compared with an initial strain, the strain has the advantages that the activity of saccharifying enzyme produced by fermentation of the strain is increased by 33%, the enzyme activity of alpha-glucose trans-glycoside enzyme is reduced by at least 43%, a separation and purification process for removing trans-glycoside enzyme from fermentation liquid is simplified, the production cost is lowered, and the recombinant bacteria have certain innovativeness and relatively great significance in development.

The invention discloses a method for recycling alkali waste liquid generated during the production of chitin and relates to the technical field of treatment and comprehensive use of wastewater generated during food processing. The method comprises the following steps: alkali waste liquid generated in a deproteinization process during the production of chitin is used as a raw material and subjected to stainless steel membrane filtration and nanofiltration to obtain clean alkali liquid to be reused; and concentrated solutions obtained after the stainless steel membrane filtration and nanofiltration are mixed, neutralized and subjected to spray drying and roller drying to obtain high-protein feed additives. Compared with the prior method for treating waste liquid, the method realizes recycling of alkali liquid, comprehensive use of active ingredients, and prevention of discharge of alkali liquid into environment, adopts a separation and purification process which is simple, reasonable, short in process, convenient in operation, high in yield and quality of alkali, and combines a stainless steel membrane filtration separation system and a nanofiltration separation system which have advantages of low operation cost, high filtration precision, high concentration factor, high concentration of the concentrated solutions and so on, contributes to production of high-protein feed additives through solidification of concentrated solutions and avoids secondary pollution.