The invention relates to a preparation method of a traditional Chinese medicine lotion for curing acute conjunctivitis, which pertains to the technical field of traditional Chinese medicine preparation methods. At present, acute conjunctivitis is cured by adopting a sulfa drug which is easy to cause gastrointestinal tract reaction or crystalluria, hematuria or tetter, antipyretic, leukocyte reduction, etc. The technical proposal of the invention is that: chrysanthemum, mint, cicada slough, mulberry leaf, fructus viticis, taraxacum mongolicum, viola yedoensis makino, herba patriniae, blackberrylily, ampelopsis japonica mak, cyrtomium fortunei, smilax glabra, bistortae, polygonum bistatum, herba lobeliae radicantis, potentilla discolor bunge, barberry root, cayratia japonica, tupan root, prunella vulgaris, clearson, seed of feather cockscomb, pale butter-fly bush bud, pipewort, equisetum hyemale, bat dung, princesplume ladysthumb fruit, and unripe licorice are taken as raw materials, the raw materials are soaked by using 1600ml water for 30mintues and boiled by small fire, residues are filtered by using gauze, thus preparing 1000ml of traditional Chinese medicine liquid which is the traditional Chinese medicine lotion for curing acute conjunctivitis. The lotion of the invention has the advantages of simple preparation method, low toxicity, fewer side effects of the prepared traditional Chinese medicine lotion and being able to directly achieve to the focus.