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Method for extracting and converting saponin in trillium tschonoskii

A technology of trillium and saponins, which is applied in the field of extraction and transformation of saponins in trillium, and can solve the problems of high process cost, cumbersome steps, and long process

Active Publication Date: 2022-06-07
嘉文丽(福建)化妆品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above method has long process, cumbersome steps and high process cost
[0004] Trillium extract has certain toxicity

Method used

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  • Method for extracting and converting saponin in trillium tschonoskii
  • Method for extracting and converting saponin in trillium tschonoskii
  • Method for extracting and converting saponin in trillium tschonoskii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0051] (1) Extraction

[0052] Take 1kg of trillium root and crush it to less than 10 mesh, use 75% ethanol as solvent, the ratio of material to liquid is 1:10, the extraction temperature is 75°C, the extraction time is 3h, and the extraction times are 2 times; the extracts are combined and concentrated in vacuo Ethanol was recovered to obtain 1.5kg of concentrate.

[0053] (2) Enzymatic hydrolysis

[0054] Weigh 30g of rhamnosidase (midsummer) enzyme solution dissolved in 2kg of pure water, add the enzyme solution to the concentrated solution, and keep it at 50°C for enzymatic hydrolysis for 48h, and the solution becomes cloudy.

[0055] (3) cooling crystallization

[0056] The enzymatic hydrolysate was cooled to 5°C and kept for 24 h, and a large amount of precipitation was precipitated; the enzymatic hydrolysate was centrifuged at 3000 g for 15 min while it was cold, and the supernatant was discarded to obtain a precipitate.

[0057] (4) Recrystallization

[0058] The pre...

Embodiment approach 2

[0062] (1) Extraction

[0063] Take 100g of trillium root and crush it to less than 10 mesh, and extract it twice with 70% ethanol. The extraction temperature is 75°C, the extraction time is 2h, and the amount of 70% ethanol is 1L; the extracts are combined, and the ethanol is recovered by vacuum concentration to obtain 200mL. Concentrate.

[0064] (2) Enzymatic hydrolysis

[0065] Weigh 50 g of naringinase (Bohao) enzyme solution dissolved in 500 mL of pure water, add the enzyme solution to the concentrated solution, and keep it at 50 °C for enzymatic hydrolysis for 24 hours, and the solution will continue to become turbid.

[0066] (3) cooling crystallization

[0067] The enzymatic hydrolysate was cooled to 5°C and kept for 24 h, and a large amount of precipitation was precipitated; the enzymatic hydrolysate was centrifuged at 3000 g for 15 min while it was cold, and the supernatant was discarded to obtain a precipitate.

[0068] (4) Recrystallization

[0069] The precip...

Embodiment approach 3

[0073] (1) Extraction

[0074] Take 10kg of trillium root and cut it into thin slices with a slicer, put it into the extraction kettle, and then put 70kg of 60% ethanol as a solvent, set the extraction temperature to 70°C and the extraction time to 3h, and extract twice; The feed liquid is pumped to the concentration kettle through an 800-mesh filter screen, and the ethanol is recovered by vacuum concentration to obtain about 30 kg of concentrated liquid.

[0075] (2) Fermentation

[0076] The strain Lactobacillus rhamnosus was first activated with MRS liquid medium for 2 to 3 generations, and then replaced part of the glucose in the MRS liquid medium with naringin for 3 to 5 generations, and finally the domesticated bacteria were used. fermented extract. The MRS liquid medium was mixed with the concentrated liquid at a ratio of 2:1, and the domesticated strain was inoculated, the fermentation temperature was 35°C, and the fermentation time was 5-7 days.

[0077] (3) Precip...

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Abstract

The invention belongs to the technical field of extraction and separation of active ingredients of natural plants, and particularly relates to an extraction and conversion method of saponin in trillium tschonoskii. According to the method, various saponins in trillium tschonoskii are converted into pennogenin-glucoside-rhamnose and diosgenin-glucoside-rhamnose by virtue of an enzymolysis method, and the saponins are crystallized by virtue of a cooling method, so that the high-purity saponins are obtained, the separation and purification process of the trillium tschonoskii saponin is simplified, and meanwhile, the cytotoxicity of the trillium tschonoskii saponin is also reduced.

Description

technical field [0001] The invention belongs to the technical field of extraction, separation and transformation of natural plant active components, and in particular relates to a method for extracting and transforming saponins in Trillium. Background technique [0002] Trillium, as the name suggests, is a herbal medicine that can prolong life. It is one of the four magic medicines of the Tujia family and is also a tertiary protected plant in my country. Trillium belongs to the genus Trillium of the Liliaceae (also considered to be the Chenopodium family). There are about 50 species of Trillium in the world, and there are only 3 species in my country, namely Trillium tschonoskii Maxim., Trillium camschatcense Ker Gawl. and Trillium govanianum Wall.ex D. Don), mainly distributed in the southwest, northwest and northeast and other places, mainly Trillium tschonoskii Maxim in Fujian. Trillium contains some special secondary metabolites, including saponins, flavonoids, sesquite...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/896A61K125/00
CPCA61K36/896A61K2236/15A61K2236/13A61K2236/333A61K2236/39A61K2236/51A61K2236/19A61K2236/53
Inventor 林晓锋曹万祥王文俊萧自智谢君君施雅丽林颖斌
Owner 嘉文丽(福建)化妆品有限公司
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