47 results about "Dioscorea futschauensis" patented technology

The invention relates to an extraction method of raw total saponins and in particular to an extraction method of raw total saponins from fresh dioscorpea plants. The extraction method of the raw total saponins from fresh dioscorpea plants mainly comprises the following steps: crushing, fermenting, radiating with microwave, performing secondary crushing, immerging into a solvent, filtering a mixedsolution and recycling the solvent. Compared with the traditional extraction method, the extraction method provided by the invention adopts a method of secondary crushing after microwave radiation, so that cellular content is quickly expanded by virtue of microwave radiation to cause sufficient expansion of cell walls accordingly, and secondary crushing is carried out to wholly disintegrate the cell walls, so that the cellular content is completely released and sufficiently contacted with the solvent, therefore, the extract can be sufficiently dissolved in the solvent, the extraction rate of a target extract is greatly improved, and the content of total saponins in a material can be over 95 percent.

The invention discloses a Bacillus subtilis WB8 capable of producing diosgenin, and belongs to the technical field of applied microbiology. The strain is separated from internal issues of subterraneous stems of a medicinal plant of Dioscorea zingiberensis wright, can synthesize and secrete a metabolin of a host plant, namely diosgenin, and has the preservation number of CCTCC (China Center For Type Culture Collection) NO:M2010271; and the strain is rod-like, has the size of 1-1.5*3-5 mu m, forms a single-chain sporocarp, releases endospore, has rough colony with grid bulges, forms pellicle through liquid fermentation, has rich foam, and can grow 16SrDNA core sequence 1542bp with the thermophilic temperature of between 30 and 35 DEG C, the pH of 6.0-9.0 and capacity of tolerating 9.0 percent NaCl, wherein the core sequence has the sequence number of HM210636. The strain liquid is fermented by a common culture medium for 48 to 60 hours, the chloroform extract of the fermentation liquor is the diosgenin, and the diosgenin has the activity of inhibiting pathogenic bacteria and resisting tumor cells. The strain provided by the invention can produce the diosgenin; and compared with the conventional process of extracting the diosgenin from yam plants, the method avoids the pollution of strong acid and yam byproducts on environment, and has potential industrial application prospect.

The invention belongs to the technical field of a microorganism secondary metabolite, and discloses a method for preparing diosgenine by a fermenting dioscorea zingiberensis endophyte. Diosgenine is obtained and separated from fermentation liquor of a dioscorea zingiberensis endophyte. The method comprises the following steps: 1) inoculating a strain culture medium with the dioscorea zingiberensis endophyte for strain culture to obtain a fermentation strain; 2) inoculating a fermentation tank containing a fermentation culture medium with the fermentation strain, starting stirring, and setting fermentation conditions of the fermentation temperature being 24-32 DEG C, the fermentation pH being 5.5-8.5, and the ventilation amount being 1.0-5.5 L / min; and 3) performing acidolysis to the obtained fermentation liquor, and carrying out neutralization to obtain diosgenine. According to a basic process, a 5L small tank is established for fermentation production of diosgenine. The yield of diosgenine is obviously increased, the technical defects of low yield of diosgenine and high energy consumption in the prior art are overcome, and research foundation is provided for development and utilization of dioscorea zingiberensis.

The invention discloses a ternary two-phase AlCl 3 The method of preparing diosgenin by hydrolysis, put the dried dioscin powder into the reaction kettle, then add the ternary biphasic solution composed of aluminum trichloride aqueous solution, ethanol and petroleum ether, evenly, react at 130-180°C for 0.5 ~3.0h, the reaction solution was filtered, the residue was filtered off, the petroleum ether layer was separated and rotary evaporated, and diosgenin was obtained after recrystallization. The present invention adopts the Lewis acid AlCl of non-traditional acid 3 The preparation of diosgenin from chrysanthemum leaf by acid hydrolysis in the ternary two-phase system not only improves the yield of saponin, shortens the extraction period, but also greatly reduces the amount of acid consumed. More importantly, without using concentrated sulfuric acid, The pollution to the environment will also become minimal, and it has great application prospects.

The invention belongs to the technical field of plant ingredient extraction, and discloses a method for preparing diosgenin from B acid DES (deep eutectic solvent) hydrolysis. Choline chloride and B acid are heated to a molten state so as to obtain a DES; dioscorea zingiberensis rhizome raw material powder, the DES and petroleum ether are added into a reactor; the temperature is raised to 100 to 130 DEG C; stirring and hydrolysis reaction are preformed; reaction liquid is subjected to filtering or centrifugal separation; a liquid petroleum ether phase is taken; petroleum ether recovered through evaporation; the diosgenin in the rest solid phase is extracted by using water saturation normal butanol solution; the normal butanol and the water are recovered through evaporation; the diosgenin is obtained. The characteristic that the B acid DES and the diosgenin can form strong hydrogen bonds is utilized, so that the DES can realize the selective extraction of the diosgenin from the raw materials for hydrolysis. The method has the advantages that the reaction selectivity is good; the pollution is low; the reaction conditions are mild; the problem of high pollution caused by simultaneoushydrolysis of cellulose and starch in the raw materials into sugar in a conventional hydrolysis process can be solved.

The invention discloses the application of diosgenin in controlling the hazards of snails in water areas, and provides a successful path for the development of novel high-efficiency and low-toxicity snail-killing products. At the same time, the present invention also provides a molluscicide prepared by compounding total alkaloids of Sophora sophora and dioscin, which is compounded with plant preparations, is environmentally friendly, safe for humans and animals, does not produce resistance, and can meet the WHO and sanitation schistosomiasis The indicator required by the competent authority is a new type of molluscicide product with high efficiency and low toxicity, which can replace the currently used molluscicide chemical niclosamide, and is worthy of application and promotion.