45 results about "Dioscorea futschauensis" patented technology

The invention discloses a Bacillus subtilis WB8 capable of producing diosgenin, and belongs to the technical field of applied microbiology. The strain is separated from internal issues of subterraneous stems of a medicinal plant of Dioscorea zingiberensis wright, can synthesize and secrete a metabolin of a host plant, namely diosgenin, and has the preservation number of CCTCC (China Center For Type Culture Collection) NO:M2010271; and the strain is rod-like, has the size of 1-1.5*3-5 mu m, forms a single-chain sporocarp, releases endospore, has rough colony with grid bulges, forms pellicle through liquid fermentation, has rich foam, and can grow 16SrDNA core sequence 1542bp with the thermophilic temperature of between 30 and 35 DEG C, the pH of 6.0-9.0 and capacity of tolerating 9.0 percent NaCl, wherein the core sequence has the sequence number of HM210636. The strain liquid is fermented by a common culture medium for 48 to 60 hours, the chloroform extract of the fermentation liquor is the diosgenin, and the diosgenin has the activity of inhibiting pathogenic bacteria and resisting tumor cells. The strain provided by the invention can produce the diosgenin; and compared with the conventional process of extracting the diosgenin from yam plants, the method avoids the pollution of strong acid and yam byproducts on environment, and has potential industrial application prospect.

The invention obtains an endophyte of a dioscorea zingiberensis by steps of field sampling, sample surface sterilization, isolation of microorganism, liquid culture and the like; the strain is identified as bacillus licheniformis, which can produce diosgenin of the dioscorea zingiberensis and can be used as production strain for fermentation.

The invention relates to a method for extracting dioscorea zingiberensis diosgenin in traditional Chinese medicine peltate yam, a preparation agent, and the application of the dioscorea zingiberensis diosgenin in preparing drugs in prevention and treatment of cardiovascular diseases. The dioscorea zingiberensis diosgenin extraction method of the invention adopts enzyme to remove coarse impurities, and then uses weak-polar macroporous resin to extract the dioscorea zingiberensis diosgenin. The method of the invention can significantly improve the purity and extraction rate of dioscorea zingiberensis diosgenin to reduce the production cost. The dioscorea zingiberensis diosgenin of the invention can also prepare coated tablets, syrup, oral solution, drops or capsules; compared with the prior art, the dioscorea zingiberensis diosgenin prepared through the method of the invention has better efficacy and is conducive to the transport and storage. Clinical trials prove that the dioscorea zingiberensis diosgenin extracted in the invention has a significant effect on coronary heart disease, hyperlipidemia and other cardiovascular diseases.

The invention belongs to the field of cosmetics and particularly relates to an anti-aging composition and preparation and application thereof. The anti-aging composition is prepared from the following ingredients in parts by weight: 1 to 3 parts of hydrolyzed pearl powder, 1 to 3 parts of dioscorea villosa root extract, 0.5 to 2 parts of acetyl hexapeptide-8, 0.5 to 2 parts of acetyl octapeptide-3 and 0.5 to 2 parts of nonapeptide-1. Experiments prove that the anti-aging composition disclosed by the invention has an extremely obvious removing effect on quiescent wrinkles, dynamic wrinkles and posture extrusion wrinkles, meanwhile can obviously promote multiplication of human skin fibroblast, extremely obviously promotes synthesis of I and III type collagen and extremely obviously inhibits expression of MMP1 and MMP3.

Production of diosgenin by super-critical hydrolysis is carried out by taking dioscorea plant as raw material, putting it into super-critical reactor, hydrolytic reacting at 200-300degree and 20-35MPa with water as solvent, filtering and extracting to obtain final product. It has no environmental pollution, higher purity and more yield.

The invention discloses a method for circularly processing and utilizing rhizome in dioscorea, which comprises the following steps: washing the rhizome in a dioscorea plant and then removing impurities, squeezing for a plurality of times by a multi-stage metal roller, circularly adding water, slurrying, filtering with screening and filtering equipment, and circularly squeezing the separated dioscorea solid residue again; mixing the filtered slurry with a polyacrylamide flocculant, stirring, allowing the obtained mixture to enter a precipitator for sedimentation separation, and separating out dioscorea starch at a lower layer; and allowing an upper suspension layer to enter a helix discharging sedimentation centrifuge for forcible separation, and then rapidly separating dioscin slurry from the settled supernatant to finally obtain concentrated dioscin. In the method for circularly processing and utilizing the rhizome in the dioscorea of the invention, the water for washing and slurrying is from the settled supernatant of the precipitator and the helix discharging sedimentation centrifuge and can be recycled. The method solves the fundamental problem of processing and utilizing the rhizome in the dioscorea and greatly improves the economic value of dioscorea application.

The invention provides a plant expression vector for synthesizing taxadiene, a strain DZGGPPSTS, dioscorea zingiberensis and a preparation method therefor. The vector contains a taxus chinensis geranylpyrophosphate synthase gene GGPPS expression cassette CaMV35S-GGPPS-Tnos and a taxus chinensis taxadiene synthase gene TS expression cassette CaMV35S2-TS-CaMV 3' UTR. The strain DZGGPPSTS is obtainedthrough transducing the vector into agrobacterium tumefaciens and has a collection number of CCTCC NO: M2019685; and a transgenic plant capable of synthesizing the taxadiene is obtained through integrating the vector into a genome of dioscorea zingiberensis through strain DZGGPPSTS mediating and carrying out regenerating. A gene GGPPS and a gene TS are over-expressed simultaneously, the dioscoreazingiberensis serves as an acceptor plant, and thus, the transgenic plant with high taxadiene synthesizing capability is obtained.

The invention discloses a dioscorea villosa anti-aging essence and a preparation method thereof. The essence includes the following components: water, a dioscorea villosa root extract, butanediol, a pueraria mirifica extract, a cimicifuga racemosa root extract, an angelica sinensis root extract, a paeonia lactiflora root extract, vitamin E, p-hydroxyacetophenone, soy isoflavone, a polygonum cuspidatum root extract, a Thailand pueraria mirifica extract, a pomegranate fruit extract, a red clover flower extract, isononyl isononanoate, squalane, cetearyl glucoside, cetearyl alcohol, a salvia miltiorrhiza root extract, a pseudo-ginseng extract, a peony root extract, a ginkgo leaf extract, 1,2-hexanediol and oligopeptide-1. The invention belongs to the technical field of anti-aging essence, and particularly relates to a dioscorea villosa anti-aging essence and a preparation method thereof, wherein the dioscorea villosa anti-aging essence can promote metabolism of skin cells, contains various active ingredients, and can promote activity of hormone secretions so as to achieve an anti-aging effect.

The invention discloses diosgenin synthesis related proteins derived from dioscorea zingiberensis as well as a coding gene and application. The diosgenin synthesis related proteins derived from the dioscorea zingiberensis provided by the invention are two proteins as shown in SEQ ID No.9 and SEQ ID No.7. A saccharomyces cerevisiae engineering strain capable of synthesizing diosgenin can be obtained by introducing diosgenin synthesis related genes derived from the dioscorea zingiberensis and a cytochrome P450 reductase coding gene into saccharomyces cerevisiae capable of synthesizing cholesterol. According to the invention, the biosynthesis of saccharomyces cerevisiae diosgenin is realized.

The invention belongs to the technical field of plant tissue culture, and provides a rapid propagation method and a culture medium for Dioscorea composita tissue culture seedlings. According to the method, the axillary buds of dioscorea composita are used as explants, callus generation is promoted on an improved MS + 2mg/L 6-BA + 0.2 mg/L NAA culture medium, callus proliferation is promoted on an MS + 2mg/L 6-BA + 0.2 mg/L NAA + 0.05% glutamic acid culture medium, redifferentiation of cluster buds is promoted on MS + 2mg/L 6-BA + 0.4 mg/L NAA + 0.01% vitamin E + 0.025% selenocysteine, and complete root structures of the axillary buds are promoted on improved MS + 1mg/L IBA + 0.1 mg/L NAA. The invention provides a technical basis for factory scale breeding of the Dioscorea mexicana, and also lays a technical basis for genetic engineering improvement and molecular breeding of the Dioscorea mexicana.

The invention discloses a preparation method of a diosgenin chitosan carrier. The preparation method is characterized by comprising the following steps of: firstly, crushing rhizomes of dioscorea zingiberensis, fermenting the rhizomes with an enzyme, and performing ultrasonic extraction with lauryl sodium sulfate and magnesium chloride to obtain an ultrasonic extracting solution; and hydrolyzing the ultrasonic extracting solution with a small amount of sulfuric acid to obtain a diosgenin hydrolysate, separating out foam with a foam separator, flushing the foam with a phosphate buffer salt solution, enabling the foam to flow into a dry lipid membrane prepared from egg yolk lecithin and cholesterol to form a liposome suspension, finally performing ultrasonic treatment, performing microfiltration, and performing chitosan wrapping to obtain the diosgenin chitosan carrier. The diosgenin yield is high, the acid consumption is low, and the diosgenin chitosan carrier is high in encapsulation efficiency, good in stability, free of irritation to human skin, high in skin permeability and high in anti-inflammatory capacity, has an inhibiting effect on tyrosinase and is applicable to cosmetics.

The invention belongs to the field of biological prevention and treatment, and particularly relates to an application of dioscin to prevention and treatment on pine nematode diseases. The dioscin disclosed by the invention is a steroid compound formed by connecting spirostane type diosgenin and a glycogen connected to a 3-site hydroxyl group through beta-1, 3 glucosidic bonds, and the dioscin comprises dioscin polyphyllin and gracillin. The dioscin can be used as an active pharmaceutical ingredient added into a pesticide carrier or composition to be prepared into a pesticide, and then the dioscin is used for preventing and treating the pine nematode disease by adopting a common pesticide formulation and a using method. The dioscin disclosed by the invention is derived from tubers of dioscorea zingiberensis serving as a dioscorea plant and is harmless to the human body and easy to degrade in environment. Compared with a conventional chemical nematocide matrine, the dioscin has the characteristics of no toxicity and no residue, has better prevention and treatment effects on pine nematode diseases, and meets the requirements of green, environment-friendly and sustainable agriculturalproduction and development.

The invention discloses applications of dioscin in treatment of water area snail harm, provides a successful path for development of a novel efficient low-toxicity snail killing product, and further relates to a snail killing preparation prepared by compounding Sophora alopecuroides total alkali and dioscin. According to the invention, the product is compounded by using the plant preparations, isenvironmentally-friendly, is safe to people and livestock, does not generate resistance, can reach the indexes required by WHO and health schistosomiasis prevention management department, is a novel efficient low-toxicity snail killing product, can replace the existing snail killing chemical niclosamide, and is worthy of application and popularization.

The invention discloses diosgenin glucoside rhamnosyltransferase as well as a coding gene and application thereof. According to the invention, a key diosgenin glucoside rhamnosyltransferase gene is separated from dioscorea zingiberensis for the first time, a gene sequence and an amino acid sequence of the key diosgenin glucoside rhamnosyltransferase gene are obtained, the diosgenin glucoside rhamnosyltransferase gene is transferred into escherichia coli for heterologous expression, and after being purified, recombinant protein can catalyze a substrate diosgenin glucoside to be converted into prosapogenin A. The diosgenin glucoside rhamnosyltransferase provides necessary gene resources for synthesis of the prosapogenin A, lays a foundation for efficient synthesis of the prosapogenin A in heterologous organisms, is also beneficial to heterologous biosynthesis of dioscin and other related steroid saponins, and has a wide application prospect.