Bacillus subtilis SWB8 for producing diosgenin

Abstract

The invention discloses a Bacillus subtilis WB8 capable of producing diosgenin, and belongs to the technical field of applied microbiology. The strain is separated from internal issues of subterraneous stems of a medicinal plant of Dioscorea zingiberensis wright, can synthesize and secrete a metabolin of a host plant, namely diosgenin, and has the preservation number of CCTCC (China Center For Type Culture Collection) NO:M2010271; and the strain is rod-like, has the size of 1-1.5*3-5 mu m, forms a single-chain sporocarp, releases endospore, has rough colony with grid bulges, forms pellicle through liquid fermentation, has rich foam, and can grow 16SrDNA core sequence 1542bp with the thermophilic temperature of between 30 and 35 DEG C, the pH of 6.0-9.0 and capacity of tolerating 9.0 percent NaCl, wherein the core sequence has the sequence number of HM210636. The strain liquid is fermented by a common culture medium for 48 to 60 hours, the chloroform extract of the fermentation liquor is the diosgenin, and the diosgenin has the activity of inhibiting pathogenic bacteria and resisting tumor cells. The strain provided by the invention can produce the diosgenin; and compared with the conventional process of extracting the diosgenin from yam plants, the method avoids the pollution of strong acid and yam byproducts on environment, and has potential industrial application prospect.

Description

technical field [0001] The invention relates to the technical field of applied microorganisms, in particular to an endogenous Bacillus subtilis SWB8 ( Bacillus subtilis SWB8). Background technique [0002] Diosgenin (Diosgenin, also known as saponin) is the hydrolyzed product of diosgenin, which is mainly used as a raw material for the synthesis of steroidal drugs, and has been widely used in cosmetics, health care, contraceptive analgesia, anesthesia, lowering cholesterol and treating diabetes, etc. drug. At present, diosgenin is mainly derived from the genus Dioscorea ( Dioscorea ), Fenugreek ( Trigonella ) and Zingiber officinalis ( Costus )plant. Dioscorea shield leaf ( Dioscorea zingiberensis C.H. Wright), also known as turmeric, is a perennial herbaceous vine, and its underground stem contains 45-50% starch, 40-50% cellulose and 2-3% diosgenin. In China, turmeric is the main source of extracting diosgenin, with a planting area of ​​1.8 million mu at its p...

AI Technical Summary

Problems solved by technology

Domestically, strong acid hydrolysis and other technologies are mainly used to extract diosgenin from turmeric plants, but the technology is backward. Statistics show that 140 tons of fresh turmeric can be processed by traditional strong acid hydrolysis, which can produce 1 ton of saponin and produce 400-500 tons of waste water, COD 30000mg / L, BOD 8000mg / L, ammonia nitrogen 300 mg / L and 10 tons of waste residue (Shen Kangrong et al., Turmeric Development and Planting Technology, Hubei Science and Technology Press), causing serious pollution to the environment

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