17 results about "Endospore Forming Bacteria" patented technology

The invention discloses a preparation method of a curing agent with biological self-healing capability, which comprises the following steps: preparing a bacillus pasteurii bacterial solution, preparing the bacillus pasteurii bacterial solution into an endophytic spore solution, immersing polyvinyl alcohol fibers into a sodium alginate / endophytic spore solution, taking out the polyvinyl alcohol fibers, immediately immersing the polyvinyl alcohol fibers into a calcium acetate solution, drying the polyvinyl alcohol fibers to obtain core fibers with a hydrogel coating, and curing the core fibers with the hydrogel coating to obtain the curing agent with biological self-healing capability. The preparation method comprises the following steps: taking magnesium phosphate cement as a raw material, immediately immersing the magnesium phosphate cement into a chloroform solution of a polystyrene and polylactic acid polymer blend, drying to obtain a coated microbial liquid, premixing urea and glucose, doping the premixed urea and glucose into the magnesium phosphate cement, uniformly stirring, adding the coated microbial liquid, and uniformly stirring to obtain the product. According to the invention, phosphate cement is adopted as the curing agent, has the advantages of high strength, short setting time, strong durability, low alkalinity, good biocompatibility and the like, and compared with the curing agent in the prior art, the curing agent not only has a good curing effect, but also has a crack self-healing function, and is especially suitable for reinforcing foundation soil.

The present invention relates to the field of bacterial protein engineering and protein fibers applicable as metal-binding bionanomaterials. More specifically, the present invention relates to engineered bacterial endospore appendage (Ena) proteins modified to contain metal-binding polypeptide (MBP) inserts providing for stable, flexible and robust protein assemblies with metal-binding activity. In particular, the invention relates to methods for designing Ena-fusion proteins capable of self-assembling into fibers, and for recombinant production of said self-assembling Ena-MBP fusion protein subunits, assemblies and fibers, ensuring a sustainable source of biological material for use in metal mineralization, metal sequestration, and metal-removal applications such as waste water treatment, water softening, or bioremediation.

The present invention relates to a method for stabilizing a liquid bacterial endophytic spore composition. The invention also relates to a multifunctional penetration preservation strategy for the suspension of the liquid bacillus endophytic spores.

The present application relates to a multifunctional-damage responsive biofiber and methods of forming thereof, comprising: a core comprising a polymeric fiber; a crosslinked endospore loaded hydrogel layer coating the core, wherein the hydrogel layer is formed with: a solution comprising endospores, one or more anionic polymers, and one or more crosslinking agents; and a (co)polymer shell encapsulating the hydrogel layer, wherein the shell has one or more layers which may be the same or different, and each of the one or more layers is formed with a (co)polymer selected from the group consisting of nitrocellulose (NITR), epoxy Resin (ER), polymethylmethacrylate (PMMA), polyvinylidene fluoride (PVDF), cyanoacrylate adhesive (CYA), polystyrene (PS), polylactic acid (PLA), and combinations thereof. This application also relates to methods for self-healing a matrix material including impregnating a matrix material with the multi-functional damage-responsive biofiber.

Disclosed herein are compositions and methods for disinfecting, sterilizing and / or sanitizing surfaces associated with human or veterinary medical uses, pharmaceutical manufacturing or cosmetic manufacturing. The method includes contacting the surface with a peracid-containing composition and a peracetic acid (PAA) enhancing composition. The methods may be used to inactivate, remove and / or prevent viruses, endophytic spores, bacteria, and combinations thereof.

The invention belongs to the technical field of plant disease prevention and treatment, and particularly relates to a method for preventing and treating plant diseases by using bacillus velezensis, which comprises the following steps: S1, strain culture and identification; the method comprises the following steps: taking a tomato greenhouse soil sample, separating bacterial strains by adopting a dilution method and a plate coating method, and screening bacterial strains with an inhibiting effect on pathogenic bacteria by adopting a plate confrontation method; the pathogenic bacteria comprise any one or more of fusarium graminearum, alternaria solani, cucumber botrytis cinerea, cucumber bacterial angular leaf spot bacteria, phytophthora capsici and peronophythora litchii. The screened strains with high bacteriostatic activity are identified as follows: morphological observation: the bacillus velezensis forms milk white and rough bacterial colonies with irregular edges on a TSA culture medium, and the thalli are gram-positive rod-shaped and are used for generating endophytic spores; the invention can alleviate the problems of environmental pollution, pesticide residue, pathogen resistance and the like caused by the existing chemical pesticide for preventing and treating plant diseases.

This invention relates to the field of microbial self-assembled protein fibrils as novel bionanomaterials. More specifically, this invention relates to protein nanofibril materials comprising Bacillus endospore appendage (ENA) proteins, which are spontaneously folded helical hairpin subunits assembled into α-helical multimers (A-ENA) fibrils having a hydrophobic core and covalently linked by one or more isopeptide bonds, providing nanofibrils with high stability and tensile strength. This invention also relates to said protein nanofibrils being engineered or modified to provide functionalized bionanomaterials. In particular, this invention relates to methods for the recombinant preparation of said self-assembled protein nanofibrils, and their use in modifying bacterial endospores and endospore activity or pathogenicity.

The invention provides a pellicle (1) comprising a matrix material (50) and an activatable material (10), wherein the activatable material (10) is configured embedded in the matrix material (50), wherein the matrix material (50) comprises bacterial cellulose (55), and wherein the activatable material (10) comprises bacterial endospores (15). The pellicle (1) may especially be used in one or more of: drug delivery, biosensing, biocatalysis, skincare, and food-packaging.

Examples of a mat are disclosed. The mat comprises a body with a first surface and a second surface, and a microbial component that comprises pre-determined microorganisms which are dispersed in the body of the mat between the first and second surfaces, on one of the first surface or the second surface, or on both of the first surface and the second surface. The microbial component manipulates a microbiome of an environment adjacent or in contact to the mat. The microbial component can comprise at least one of live bacteria, bacteria endospores, fungi, viruses, protozoa, algae, slime moulds, archaca, micro-plants, a synthetic biology system or microbial precursor that stimulate growth of the desired microorganisms.

The present invention relates to methods for stabilizing liquid bacterial endospores compositions. The present invention also relates to multi-functional osmotic preservation strategies for liquid Bacillus endospore suspensions.

The present invention relates to a method for reducing bacterial endospores in an aqueous fiber suspension containing recycled cellulosic fibers, the fiber suspension preferably having an original endospore load of 10,000 CFU / ml or more. The method includes adjusting the pH of the fiber suspension to a pH value of 6.5 or less, adjusting the oxidation-reduction potential (ORP) of the fiber suspension to an ORP value of 200 mV or more using a first oxidizing agent, and introducing an amount of performic acid as a second oxidizing agent into the fiber suspension to reduce the bacterial endospores to an endospore load of 1,000 CFU / ml or less.

The invention relates to a method for reducing bacterial endospores in an aqueous fibre suspension comprising recycled cellulosic fibres, wherein the fibre suspension has an original endospore amount, preferably of ≥10 000 CFU / ml. The method comprises adjusting the pH of the fibre suspension to a pH value of ≤6.5, adjusting the oxidation-reduction potential (ORP) of the fibre suspension to an ORP value of ≥200 mV with a first oxidizing agent, and introducing an amount of performic acid as a second oxidizing agent to the fibre suspension for reducing the bacterial endospores to an endospore amount of ≤1000 CFU / ml.

The present invention relates to concrete compositions and methods of making a concrete composition having self-healing properties, including a cementitious matrix impregnated with a plurality of biofibers, wherein the biofibers comprise: a polymer fiber core comprising one or more polymers selected from the group consisting of polyester, polyethylene, polypropylene, polyvinyl alcohol, polyamide, aramid, polyacrylonitirile, cellulose, polyurethane, and combinations thereof; a crosslinked hydrogel layer coating on the polymer fiber core, wherein the hydrogel layer comprises endospores, one or more cross-linked anionic polymers, and a (co)polymer shell encapsulating the hydrogel layer, wherein the shell has one or more layers which may be the same or different, and each of the one or more layers is formed with a (co)polymer selected from the group consisting of nitrocellulose (NITR), epoxy resin (ER), polymethyl methacrylate(PMMA), polyvinylidene fluoride (PVDF), cyanoacrylate adhesive (CYA), polystyrene (PS), polylactic acid (PLA), and combinations thereof.

This invention discloses a high-purity, multifunctional probiotic powder and its preparation method. Based on the different characteristics of lactic acid bacteria, Bacillus subtilis, and actinomycetes, this invention employs different methods for their respective proliferation. This not only avoids potential mutual interference and contamination during their individual proliferation processes but also achieves high viable cell counts while maintaining high purity, and the high viable cell count is maintained even after mixing. This invention mixes the proliferated bacterial powders in a reasonable ratio, allowing lactic acid bacteria to be the dominant strain while fully utilizing the functional characteristics of each of the three bacteria: lactic acid bacteria are anaerobic, while Bacillus subtilis and actinomycetes are aerobic. The latter can absorb oxygen released by the former, ensuring that in the anaerobic environment of the human intestine, Bacillus subtilis and actinomycetes remain active rather than dormant, generating resistant endospores through synergistic action with lactic acid bacteria.

The present invention discloses a method for preparing a curing agent with biological self-healing ability, which comprises the following steps: first preparing a bacillus pasteurianus liquid, then preparing an endospore solution, then immersing a polyvinyl alcohol fiber in a sodium alginate / endospore solution, taking it out and immediately immersing it in a calcium acetate solution, drying it, obtaining a core fiber with a hydrogel coating, immediately immersing it in a chloroform solution of a polystyrene and polylactic acid polymer blend, drying it, obtaining a coated microbial liquid, pre-mixing urea and glucose, adding them to magnesium phosphate cement, stirring them evenly, then adding the coated microbial liquid, stirring them evenly, and obtaining the product. The present invention adopts phosphate cement as a curing agent, which has the advantages of high strength, short setting time, strong durability, low alkalinity, good biocompatibility, etc. Compared with the curing agent in the prior art, the curing agent not only has a good curing effect, but also has a crack self-healing function, and is particularly suitable for reinforcing foundation soil.

The present invention provides a method and a system for detecting the presence of an analyte in a sample. In particular, the present invention provides a system, such as a diagnostic kit, for detecting the presence of an analyte in a sample, comprising (a) a recombinant bacterium or spore expressing one or more recombinant proteins on the surface thereof, wherein the recombinant protein specifically binds to the analyte directly or through a binding agent that specifically binds to the recombinant protein and the analyte, and (b) a signal-producing substance that can be detected.