587 results about "Bacteriocidal Agents" patented technology

The invention relates to bacillus, a biocontrol inoculant prepared from the bacillus and application of the biocontrol inoculant, and belongs to the technical field of biological inoculants. The bacillus provided by the invention is bacillus subtilis and is named as ZZ1, the preservation unit is China General Microbiological Culture Collection Center, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, the preservation center is numbered as CGMCC No.31374, and the preservation time is July 19, 2024. The bacillus ZZ1 disclosed by the invention has a certain bacteriostatic effect on different pathogenic bacteria regardless of living bacteria, supernate or cell lysis solution. The bacillus ZZ1 is high in living ability and wide in application range of nutrient sources, temperature, humidity and pH value. The compound has no pathogenicity to fruits and vegetables, has good tolerance to broad-spectrum bactericides, and has a great application prospect.

The invention discloses a microbial agent for preventing and treating soil-borne diseases of food crops and a preparation method of the microbial agent, and belongs to the technical field of microorganisms. The microbial inoculum comprises three functional strains, namely pseudomonas guayensis, paenibacillus polymyxa and trichoderma koningiopsis, the three strains are mixed in equal proportion and cooperate with one another, so that the microbial inoculum has a remarkable disease-resistant effect, has an antagonistic effect on various diseases of wheat and corn, can be used for preventing and treating wheat root rot, take-all and banded sclerotial blight as well as corn stem rot and banded sclerotial blight, and has a wide application prospect. Particularly, the inhibition effect on the take-all disease of wheat is optimal. Meanwhile, the coating agent and the auxiliaries are used, so that the acting durability and stability of the microbial inoculum are improved. The microbial agent is matched with a conventional base fertilizer for use, use of chemical bactericides is reduced, growth of common pathogenic bacteria of food crops is effectively inhibited, and the yield of the food crops is increased.

The invention relates to bacillus, a biocontrol inoculant prepared from the bacillus and application of the biocontrol inoculant, and belongs to the technical field of biological inoculants. The bacillus provided by the invention is bacillus subtilis and is named as QY2, the preservation unit is China General Microbiological Culture Collection Center, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, the number of the preservation center is CGMCC No.31373, and the preservation time is July 19, 2024. The bacillus QY2 disclosed by the invention has a certain bacteriostatic effect on different pathogenic bacteria regardless of living bacteria, supernate or cell lysis solution. The bacillus QY2 is high in living ability and wide in application range of nutrient sources, temperature, humidity and pH value. The compound has no pathogenicity to fruits and vegetables, has good tolerance to broad-spectrum bactericides, and has a great application prospect.

The invention relates to bacillus velezensis, a biocontrol inoculant prepared from the bacillus velezensis and application of the biocontrol inoculant, and belongs to the technical field of biological inoculants. The bacillus velezensis provided by the invention is bacillus velezensis and is named as JK1, the preservation unit is China General Microbiological Culture Collection Center, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing, the number of the preservation center is CGMCC No.31375, and the preservation time is July 19, 2024. The bacillus JK1 disclosed by the invention has relatively excellent bacteriostatic action on different pathogenic bacteria regardless of living bacteria, supernate or cell lysis solution. The bacillus JK1 is high in living ability and wide in application range of nutrient sources, temperature, humidity and pH value. The compound has no pathogenicity to fruits and vegetables, has good tolerance to broad-spectrum bactericides, and has a great application prospect.

The invention relates to bacillus amyloliquefaciens, a biocontrol inoculant prepared from the bacillus amyloliquefaciens and application of the biocontrol inoculant, and belongs to the technical field of biological inoculants. The bacillus amyloliquefaciens provided by the invention is bacillus amyloliquefaciens and is named as JK2, the preservation unit is China General Microbiological Culture Collection Center, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, the number of the preservation center is CMCC No.31376, and the preservation time is July 19, 2024. The bacillus amyloliquefaciens JK2 disclosed by the invention has a certain bacteriostatic effect on different pathogenic bacteria regardless of living bacteria, supernate or cell lysis solution. The bacillus amyloliquefaciens JK2 is high in living ability and wide in application range of nutrient sources, temperature, humidity and pH value. The compound has no pathogenicity to fruits and vegetables, has good tolerance to broad-spectrum bactericides, and has a great application prospect.

The invention discloses a novel acinetobacter baumannii phage vBAbaSqsb1 and application thereof, and belongs to the technical field of microorganisms. The bacteriophage is an Acinetobacter baumannii bacteriophage vBAbaSqsb1 which is preserved in the Guangdong Microbiological Culture Collection Center on July 5, 2024, the preservation number is GDMCC (China General Microbiological Culture Collection Center) NO: 64831-B1, and the preservation address is Institute of Microbiology of Academy of Sciences of Guangdong Province. The bacteriophage can specifically recognize and split the acinetobacter baumannii so as to eliminate infection and overcome the problem of antibiotic resistance of the acinetobacter baumannii. Through discovery and application research of the phage vBAbaSqsb1, the phage vBAbaSqsb1 not only can be used for preparing a phage preparation for preventing and treating diseases caused by acinetobacter baumannii infection, but also can be used for preparing various environmental bactericides for solving potential acinetobacter baumannii in various environments. The bacteriophage vBAbaSqsb1 has the characteristics of safety, no toxic or side effect and the like, so that the problem of drug resistance caused by abuse of antibiotics can be avoided when the bacteriophage vBAbaSqsb1 is applied.

The invention discloses a self-response odor-removing and sterilizing material, a preparation method thereof and a refrigerator, and belongs to the technical field of refrigerator adsorption materials. Comprising main materials and auxiliary materials, the main body material is composed of an adsorption material and a formaldehyde removal agent; the auxiliary material is composed of a response material and a bactericide; the response material is selected from a covalent organic framework; the bactericide is selected from benzimidazole compounds; the benzimidazole compound shows a deprotonation trend when the pH value is any one value of 2.8-7.1, and is combined with the covalent organic framework through electrostatic attraction; when the pH value is less than 2.8, the benzimidazole compound and the covalent organic framework are protonated at the same time, and the benzimidazole compound is released. The sterilization material is applied to the refrigerator sterilization aspect, and the problems that the sterilization effect is not lasting due to the fact that a common odor-removing sterilization material plays a role to release sterilization substances from the beginning of application, or the sterilization material cannot be actively released, the sterilization effect can be achieved only when bacteria are adsorbed on the surface of the sterilization material, and the bacteria in the air cannot be killed are solved.

The invention discloses application of threonine dehydratase in regulation and control of growth of Didymeria segeticola and application of threonine dehydratase serving as a bactericide target in regulation and control of growth of Didymeria segeticola. The threonine dehydratase gene Td disclosed by the invention is derived from Didymeria segeticola, and a knockout mutant is obtained by constructing a knockout gene segment of the Td gene and introducing the knockout gene segment into a D.segeticola protoplast. Experiments find that the Td gene plays a role in growth of D.segeticola, in-vivo pyruvic acid content, in-vivo ATP (adenosine triphosphate) content and sensitivity to wuyiencin. Molecular docking analysis, molecular dynamics simulation and trace thermophoresis experiments prove that the threonine dehydratase can be used as a bactericide target, and the bactericide wuyiencin is obtained through screening. The threonine dehydratase disclosed by the invention can be used as a bactericide target and a key protein of a disease pathogenic mechanism, and has a wide application prospect in prevention and treatment of plant pathogenic fungal diseases.

The invention discloses arginine methyltransferase protein from phytophthora plant pathogenic oomycetes as well as a coding gene and application thereof. The arginine methyltransferase protein provided by the invention is as shown in sequences 6-8; the coding gene is shown in sequences 1-3. Experiments prove that the protein disclosed by the invention plays an important role in growth, development and pathopoiesis processes of typical phytophthora plant pathogen oomycete phytophthora capsici, and after the protein is deleted or the expression level is reduced, the growth rate of phytophthora capsici hyphae is reduced, the sporangium yield is reduced, the number of zoospore is reduced, the pathogenicity is reduced and the like. Corresponding arginine methyltransferase inhibitors have different degrees of activity on typical plant pathogenic oomycetes such as phytophthora capsici, phytophthora sojae and phytophthora infestans. The gene provided by the invention has great application potential in the aspect of preventing and controlling the crop epidemic diseases caused by phytophthora plant pathogenic oomycetes, and a novel bactericide developed based on the protein as an action target has important practical significance in controlling the occurrence and prevalence of the crop epidemic diseases caused by phytophthora.

The invention relates to bacillus subtilis, a biocontrol inoculant prepared from the bacillus subtilis and application of the biocontrol inoculant, and belongs to the technical field of biological inoculants. The bacillus provided by the invention is bacillus subtilis and is named as KC2, the preservation unit is China General Microbiological Culture Collection Center, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, the number of the preservation center is CGMCC No.31372, and the preservation time is July 19, 2024. The bacillus KC2 disclosed by the invention has a certain bacteriostatic effect on different pathogenic bacteria regardless of living bacteria, supernate or cell lysis solution. The bacillus KC2 is high in living ability and wide in application range of nutrient sources, temperature, humidity and pH value. The compound has no pathogenicity to fruits and vegetables, has good tolerance to broad-spectrum bactericides, and has a great application prospect.

The invention belongs to the technical field of modern agriculture, and particularly relates to a biocontrol bacillus amyloliquefaciens BA200 and application thereof. A new biological control bacterium BA200 is separated and identified, is named as bacillus amyloliquefaciens by taxonomy, and has a remarkable inhibition capability on two pathogenic bacteria, namely fusarium oxysporum and phytophthora vigna, causing the cowpea fusarium wilt, and the inhibition rate is up to 60% or above; fermentation liquor prepared from the bacillus amyloliquefaciens BA200 can remarkably reduce the morbidity and disease index of cowpea fusarium wilt, is a good biocontrol microorganism and has application and development potential and important significance in control of cowpea fusarium wilt, and the biocontrol bacterium bacillus amyloliquefaciens BA200 has a broad-spectrum biological control effect, can inhibit various phytopathogens and has a good application prospect. And valuable biocontrol bacterium resources are provided for preparation of biological bactericides.

To provide a novel pest control agent composition, particularly a bactericidal composition.SOLUTION: A bactericidal or bacteriostatic composition comprising an active ingredient A selected from pyrazole compounds or salts thereof represented by formula (I): [G represents G-1; G1 represents C1-C6 haloalkyl or the like; T represents an oxygen atom or the like; RX represents C1-C6 alkyl or the like; RY represents a hydrogen atom or the like; R1 represents a hydrogen atom or the like; R2 represents a hydrogen atom or the like; R3 represents a hydrogen atom or the like; Ra represents a hydrogen atom or the like; Z represents Z-1 or the like; Z1 represents C1-C6 alkyl or the like; m5 and n5 each represent an integer of 0, 1, 2, 3, 4, or 5; and p represents an integer of 0 or 1] and an active ingredient B selected from known bactericidal or bacteriostatic compounds.SELECTED DRAWING: None

The invention discloses a CsbHLH118 gene and application of the CsbHLH118 gene in regulation of citrus sour rot, and belongs to the technical field of gene engineering. The nucleotide sequence of the transcription factor CsbHLH118 is shown as SEQ ID NO.1. The transcription factor CsbHLH118 provided by the invention has the advantages that the occurrence of the sour rot of the navel oranges can be delayed by transient overexpression of the CsbHLH118, the resistance to sour rot bacteria is lost by transient silence of the CsbHLH118, and the CsbHLH118 plays a positive regulation role in the resistance to the postharvest sour rot of the navel oranges. The invention provides a target gene for developing effective bactericides.

The invention relates to the technical field of bactericides, and discloses an oilfield flooding bactericide containing plant bactericidal components and a preparation method thereof. The preparation method comprises the following steps: loading the plant-based traditional Chinese medicine extract and the isothiazolinone-based corrosion inhibitor on the silver-loaded cyclodextrin metal organic framework to prepare an antibacterial agent / silver-loaded cyclodextrin metal organic framework composite material, and mixing the antibacterial agent / silver-loaded cyclodextrin metal organic framework composite material with methyl methacrylate to prepare the antibacterial agent / silver-loaded cyclodextrin metal organic framework composite material. The bactericide obtained by carrying out copolymerization reaction on modified hydroxyquinoline, vinyl quaternary phosphonium salt and methacrylic acid through an in-situ polymerization method can effectively kill and inhibit the breeding of sulfate reducing bacteria and other bacteria in oilfield reinjection water, and has a certain corrosion inhibition effect.

The invention relates to the technical field of reverse osmosis membrane maintenance, in particular to a cleaning scale inhibitor special for a reverse osmosis membrane and application of the cleaning scale inhibitor. The cleaning scale inhibitor provided by the invention comprises an agent A, an agent B and an agent C, the agent A comprises the following components in percentage by mass: 10-20% of organic phosphonic acid, 10-20% of polycarboxylic acid polymer, 5-10% of sulfamic acid, 3-8% of sodium gluconate and the balance of water; the agent B comprises 8-15% of an isothiazolinone bactericide, 5-10% of a nonionic surfactant, 3-8% of a chlorine bactericide, 2-5% of sodium dodecyl benzene sulfonate and the balance of water; and the agent C comprises 10-20% of sodium polyacrylate, 8-15% of sodium hexametaphosphate, 5-10% of citric acid, 3-8% of silicate and the balance of water. The cleaning scale inhibitor provided by the invention has an efficient cleaning effect on various pollutants, is environment-friendly, and does not cause chemical damage to a reverse osmosis membrane.

The invention discloses a function of a zinc finger transcription factor in regulating resistance of plant pathogenic fungi to various bactericides, which is a transcription factor with a zinc finger structure obtained from botrytis cinerea and named as Bcmr1. After the transcription factor is over-expressed in a botrytis cinerea standard strain B05.10, the sensitivity of pathogenic bacteria to bactericides with various action mechanisms is reduced, and the sensitivity of the pathogenic bacteria to the bactericides after deletion of the transcription factor is remarkably improved. The invention provides a synergist screening candidate target for the sensitivity recovery of the phytopathogen with multidrug resistance in the field to the bactericide, and is beneficial to the treatment of the multidrug resistance of the phytopathogen and the improvement of the activity of the existing bactericide.

The invention discloses a novel bradyrhizobium strain SBM1 as well as a preparation and application thereof, and belongs to the technical field of microorganisms. The novel bradyrhizobium strain SBM1 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.29121. The strain has good tolerance to herbicides, bactericides and insecticides, and is high in nodulation and nitrogen fixation capacity, wide in symbiotic spectrum and remarkable in nitrogen-saving and high-yield-promoting capacity. Under the field planting condition that the application amount of nitrogen fertilizer is reduced by 50%, the strain can remarkably improve the nitrogen fixation and growth performance of crops, so that a strain source is provided for obtaining a rhizobium inoculant suitable for planting various varieties of peanuts, the application amount of the nitrogen fertilizer is reduced, normal implementation of crop pesticides is guaranteed, and the economic benefit is increased. The important significance is realized on the increase of the crop yield.

The invention relates to the technical field of fertilizers, and discloses a rice sheath blight disease prevention fertilizer, which comprises a fertilizer, medium trace elements, an adhesion functional carrier, a functional additive, and a microbial agent or a bactericide, the fertilizer comprises a nitrogen-phosphorus-potassium compound fertilizer, an original fertilizer, calcium superphosphate and ammonium sulfate, wherein the original fertilizer comprises decomposed livestock and poultry manure, crop straw compost, bean cakes and rape seed cakes; the medium trace elements comprise boric acid, copper sulfate and borax; the microbial agent comprises a bacillus subtilis microbial agent and a trichoderma harzianum microbial agent; the bactericide comprises validamycin wettable powder, a thifluzamide suspending agent and a tebuconazole emulsion in water. Through the components of the fertilizer, the occurrence rate of rice sheath blight disease can be remarkably reduced, the yield and quality of rice can be improved, the microbial agent can increase the number and activity of beneficial microorganisms in soil, the soil structure and fertility are improved, and the yield and quality of rice are improved. A good soil condition is created for rice growth, so that the rice has a better resistance effect.

The invention is suitable for the technical field of agricultural bactericides, and provides application of an echinocandin antifungal drug in prevention and treatment of agricultural diseases. According to the present invention, the two common echinocandin antifungal drugs such as the anidulafungin and the Rezafungin acetate, which are common in the field of medicine, are firstly applied to prevention and control of crop diseases; prevention and control tests are carried out on crop fungal diseases such as sclerotiniose, corn southern leaf blight, tomato gray mold, rice blast and crop anthracnose, and results show that the two drugs can significantly inhibit the growth of pathogenic fungal hyphae, and compared with an existing agricultural commercial bactericide carbendazim, the bactericidal composition shows higher inhibitory activity and has the characteristics of high efficiency and low toxicity. The invention lays a foundation for deep research and application of echinocandin drugs in the agricultural field, has a wide market prospect, effectively improves the crop disease prevention and control level, and promotes grain yield increase and quality safety.

The invention discloses an ADF protein from phytophthora as well as a coding gene and application thereof. According to the present invention, the PsADF protein provided by the present invention is the protein represented by sequences 2 and 5 in Phytophthora sojae (Phytophthora sojae); the coding gene is shown as a sequence 3 and a sequence 4. Tests prove that the protein provided by the invention plays an important role in the growth and development process of phytophthora sojae, specifically, after the 64-site mutation of the key amino acid functional site of the protein, the sporangium abnormality and zoospore quantity of the phytophthora sojae are reduced, and the pathogenicity is reduced. The protein provided by the invention has very high application value in control of epidemic disease occurrence and epidemic caused by phytophthora, and provides a molecular target for research and development of novel bactericides in the future. The corresponding ADF inhibitors have different degrees of activity on typical plant pathogenic oomycetes such as phytophthora sojae, phytophthora capsici and phytophthora infestans. The protein provided by the invention has great application potential in the aspect of preventing and controlling the crop epidemic diseases caused by phytophthora plant pathogenic oomycetes, and a novel bactericide developed based on the protein as an action target has important practical significance in controlling the occurrence and prevalence of the crop epidemic diseases caused by phytophthora.

The invention discloses a disinfection tank for wastewater treatment, and belongs to the field of wastewater treatment.The disinfection tank comprises a disinfection tank body, and a liquid inlet device is fixedly installed on one side of the disinfection tank body; the rapid decomposition assembly comprises a heating plate fixedly mounted on the bottom wall of an inner cavity of the disinfection tank, a square rod is rotatably mounted on the bottom wall of the inner cavity of the disinfection tank, a sleeve frame sleeves the outer side of the square rod, a cylinder is rotatably mounted on the top wall of the inner cavity of the disinfection tank, a reciprocating screw rod is arranged in an inner cavity of the cylinder, and a rotating shaft is arranged in the inner cavity of the cylinder; the reciprocating screw rod is rotatably mounted with the disinfection tank and the cylinder, and a movable plate is slidably mounted in an inner cavity of the cylinder; according to the sterilizing tank, wastewater and a sterilizing agent in the sterilizing tank can be uniformly heated, and the process of decomposing the sterilizing agent into active ingredients can be accelerated by combining heating with efficient mixing, so that the residual sterilizing agent which is not completely reacted and the generation of potential pollutants are reduced, and the sterilizing efficiency is further improved.

The invention provides a cleaning agent with double effects of cleaning and sterilization for a scrubber and a preparation method of the cleaning agent. The cleaning agent comprises the following components in parts by weight: 5-10 parts of a solvent, 10-20 parts of a surfactant, 0.5-3 parts of a washing assistant, 0-1 part of a pH regulator, 1.5-3 parts of a bactericide, 0.3 part of essence, 0.01-0.1 part of an organic silicon emulsion and the balance of deionized water, wherein the total parts by weight of the components are 100. According to the invention, low-foam and foam-inhibiting components and long-acting antibacterial components are added, and the surfactant matched with the system and the addition amount of the surfactant are adjusted at the same time, so that while the cleaning and decontamination capabilities are ensured, the cleaning agent is endowed with strong bactericidal capability and long-acting antibacterial performance, the interior and exterior of the scrubber equipment are not damaged, and the occurrence of the situation that the equipment is mistakenly reported and halted is avoided.

The invention discloses a CsMUTE gene and application thereof in regulation of citrus sour rot, and belongs to the technical field of gene engineering. The nucleotide sequence of the gene is as shown in SEQ ID NO. 1. The invention provides a transcription factor CsMUTE, and experiments find that transient overexpression of CsMUTE can delay the occurrence of sour rot of navel oranges, transient silence of CsMUTE loses resistance to sour rot bacteria, and CsMUTE plays a positive regulation role in resistance of postharvest sour rot of navel oranges. The invention provides a target gene for developing effective bactericides.

The invention relates to the field of biology, in particular to phytophthora sojae psFBP21 protein, a psFBP21 gene, a biological material and application thereof. The amino acid sequence of the phytophthora sojae psFBP21 protein provided by the invention is as shown in SEQ ID NO. 2; the nucleotide sequence of the coding gene is as shown in SEQ ID NO. 1. Experiments prove that the phytophthora sojae psFBP21 protein provided by the invention plays an important role in the growth and development process of phytophthora sojae, specifically, after the protein is deleted, the growth of phytophthora sojae hyphae is slowed down, the number of oospore is reduced, the pathogenicity is reduced and the like. The conclusion provides a technical basis for exploring phytophthora sojae development and a pathogenic molecular mechanism, and provides a molecular target for research and development of novel bactericides in the future.

The present invention (named: a class of aminonaphthoquinone derivatives and their preparation and sterilization, weeding and algae killing uses) relates to 33 2-chloro-3-aminonaphthoquinone formate, urea, amide and sulfonamide derivatives and their preparation methods, as well as their uses in sterilization, weeding and algae killing. The 33 2-chloro-3-aminonaphthoquinone formate, urea, amide and sulfonamide derivatives are generated by reacting 2,3-dichloronaphthoquinone with potassium cyanate, substituted alcohol or amine, or with sulfonamide; or by reacting 2-chloro-3-aminonaphthoquinone with different amides. These compounds can be used as fungicides to control tomato gray mold, or as herbicides to control weeds such as Amaranthus retroflexus and Crabgrass, or as algaecides to control algae.

The utility model discloses a sterile filling device for rod-inserting food, which comprises a sterile bin, a conveying part, a rod-inserting part, a filling part, a sealing part, a sterilizing part and a drying part, and the sterilizing part, the drying part, the rod-inserting part, the filling part and the sealing part are sequentially distributed on a conveying path. The sterile bin is provided with a sterilizing cavity, a drying cavity, a rod inserting cavity, a filling cavity and a sealing cavity, the sterilizing component extends into the conveying path through the sterilizing operation end to spray a sterilizing agent to the conveying path, and the drying component extends into the conveying path through the drying operation end to spray drying gas to the conveying path. According to the utility model, the top of the sterilized forming sheet sequentially extends into and passes through the rod insertion cavity, the filling cavity and the sealing cavity, so that the use amount of sterile air required to be introduced for constructing the whole sterile environment of the sterile bin is reduced, the construction conditions of the sterile environment are reduced, and the production cost is greatly reduced.

The invention discloses bacillus subtilis LXN-1, and the bacillus subtilis LXN-1 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC.NO 31697. The bacillus subtilis LXN-1 is a bacillus subtilis LXN-1 strain. The bacillus subtilis LXN-1 has an inhibition effect on various plant pathogenic bacteria, the inhibition rate on tomato botrytis cinerea can reach 61.5%, the inhibition rate on tobacco brown spot caused by alternaria longibrachiata can reach 72%, the bacillus subtilis LXN-1 also has a virus resistance effect, the inhibition rate on tobacco TMV viral plaque can reach 95%, and the bacillus subtilis LXN-1 has a good application prospect. In addition, the bacillus subtilis LXN-1 also has the potential of being compounded with pesticide bactericides with iprodione, trifloxystrobin, difenoconazole, pyraclostrobin, copper oxychloride and copper hydroxide as main components for use, and has market popularization and application values, and absorption of the plants to nutrient elements can be enhanced, and the growth of the plants can be promoted, and the bacillus subtilis LXN-1 also has the potential of being compounded with the pesticide bactericides with the iprodione, the trifloxystrobin, the difenoconazole, the pyraclostrobin, the copper oxychloride and the copper hydroxide as main components.

The invention discloses a method for detecting drug resistance of botrytis cinerea to succinate dehydrogenase (SDHI) bactericides. On the basis of an RPA-CRISPR / Cas12a system, a specific primer pair amplification target fragment and crRNA for activating Cas12a trans-cleavage activity are designed aiming at key mutation sites related to drug resistance in a bactericide action target SdhB gene, so that fluorescent light visible to naked eyes can be generated only when a resistant strain sequence is matched with the crRNA, and the fluorescent light is opposite to the fluorescent light generated when a sensitive strain sequence is matched with the crRNA. The method provided by the invention overcomes the dependence on laboratories and large instruments in the traditional drug resistance detection method, provides key technical support for accurate prevention and control and drug resistance management of grape gray mold, and provides direct drug use basis for scientific prevention and control in the field.

According to the carbapenem-resistant klebsiella pneumoniae bacteriophage and the application, biological characteristic research, morphological analysis and other analysis are carried out on the bacteriophage, and it is found that the bacteriophage has a wide application prospect as a novel potential therapeutic agent and bactericide; meanwhile, the invention also provides a new feasible method for infection control and related application of clinical carbapenem-resistant klebsiella pneumoniae by researching the in-vitro synergistic effect of the klebsiella pneumoniae after the klebsiella pneumoniae is combined with carbapenem antibiotic meropenem.

The invention relates to a seawater-based delayed cross-linking agent, the seawater-based delayed cross-linking agent is a poly (ammonia boron) cross-linking agent, and the poly (ammonia boron) cross-linking agent is one of a poly (ammonia boron) cross-linking agent I and a poly (ammonia boron) cross-linking agent II; the seawater-based ultra-low-concentration guanidine gum fracturing fluid is prepared from the following components in percentage by mass: 0.20 to 0.35 percent of a vegetable gum thickening agent, 0.20 to 0.40 percent of a seawater-based delayed cross-linking agent, 0.10 to 0.30 percent of a seawater-based cross-linking synergist, 0.05 to 0.10 percent of a pH (Potential of Hydrogen) regulator, 0.05 to 0.10 percent of a cleanup additive, 0.2 to 3 percent of an anti-swelling agent, 0.03 to 0.20 percent of a sterilizing agent and the balance of seawater, according to the invention, the water cost of fresh water can be saved, the transportation cost of transporting fresh water can also be saved, and the effect of saving the cost of fracturing fluid is especially remarkable.