394 results about "Bacteriocidal Agents" patented technology

The invention discloses a microbial agent for preventing and treating soil-borne diseases of food crops and a preparation method of the microbial agent, and belongs to the technical field of microorganisms. The microbial inoculum comprises three functional strains, namely pseudomonas guayensis, paenibacillus polymyxa and trichoderma koningiopsis, the three strains are mixed in equal proportion and cooperate with one another, so that the microbial inoculum has a remarkable disease-resistant effect, has an antagonistic effect on various diseases of wheat and corn, can be used for preventing and treating wheat root rot, take-all and banded sclerotial blight as well as corn stem rot and banded sclerotial blight, and has a wide application prospect. Particularly, the inhibition effect on the take-all disease of wheat is optimal. Meanwhile, the coating agent and the auxiliaries are used, so that the acting durability and stability of the microbial inoculum are improved. The microbial agent is matched with a conventional base fertilizer for use, use of chemical bactericides is reduced, growth of common pathogenic bacteria of food crops is effectively inhibited, and the yield of the food crops is increased.

The invention relates to bacillus velezensis, a biocontrol inoculant prepared from the bacillus velezensis and application of the biocontrol inoculant, and belongs to the technical field of biological inoculants. The bacillus velezensis provided by the invention is bacillus velezensis and is named as JK1, the preservation unit is China General Microbiological Culture Collection Center, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing, the number of the preservation center is CGMCC No.31375, and the preservation time is July 19, 2024. The bacillus JK1 disclosed by the invention has relatively excellent bacteriostatic action on different pathogenic bacteria regardless of living bacteria, supernate or cell lysis solution. The bacillus JK1 is high in living ability and wide in application range of nutrient sources, temperature, humidity and pH value. The compound has no pathogenicity to fruits and vegetables, has good tolerance to broad-spectrum bactericides, and has a great application prospect.

The invention relates to bacillus amyloliquefaciens, a biocontrol inoculant prepared from the bacillus amyloliquefaciens and application of the biocontrol inoculant, and belongs to the technical field of biological inoculants. The bacillus amyloliquefaciens provided by the invention is bacillus amyloliquefaciens and is named as JK2, the preservation unit is China General Microbiological Culture Collection Center, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, the number of the preservation center is CMCC No.31376, and the preservation time is July 19, 2024. The bacillus amyloliquefaciens JK2 disclosed by the invention has a certain bacteriostatic effect on different pathogenic bacteria regardless of living bacteria, supernate or cell lysis solution. The bacillus amyloliquefaciens JK2 is high in living ability and wide in application range of nutrient sources, temperature, humidity and pH value. The compound has no pathogenicity to fruits and vegetables, has good tolerance to broad-spectrum bactericides, and has a great application prospect.

The invention discloses a self-response odor-removing and sterilizing material, a preparation method thereof and a refrigerator, and belongs to the technical field of refrigerator adsorption materials. Comprising main materials and auxiliary materials, the main body material is composed of an adsorption material and a formaldehyde removal agent; the auxiliary material is composed of a response material and a bactericide; the response material is selected from a covalent organic framework; the bactericide is selected from benzimidazole compounds; the benzimidazole compound shows a deprotonation trend when the pH value is any one value of 2.8-7.1, and is combined with the covalent organic framework through electrostatic attraction; when the pH value is less than 2.8, the benzimidazole compound and the covalent organic framework are protonated at the same time, and the benzimidazole compound is released. The sterilization material is applied to the refrigerator sterilization aspect, and the problems that the sterilization effect is not lasting due to the fact that a common odor-removing sterilization material plays a role to release sterilization substances from the beginning of application, or the sterilization material cannot be actively released, the sterilization effect can be achieved only when bacteria are adsorbed on the surface of the sterilization material, and the bacteria in the air cannot be killed are solved.

The invention discloses application of threonine dehydratase in regulation and control of growth of Didymeria segeticola and application of threonine dehydratase serving as a bactericide target in regulation and control of growth of Didymeria segeticola. The threonine dehydratase gene Td disclosed by the invention is derived from Didymeria segeticola, and a knockout mutant is obtained by constructing a knockout gene segment of the Td gene and introducing the knockout gene segment into a D.segeticola protoplast. Experiments find that the Td gene plays a role in growth of D.segeticola, in-vivo pyruvic acid content, in-vivo ATP (adenosine triphosphate) content and sensitivity to wuyiencin. Molecular docking analysis, molecular dynamics simulation and trace thermophoresis experiments prove that the threonine dehydratase can be used as a bactericide target, and the bactericide wuyiencin is obtained through screening. The threonine dehydratase disclosed by the invention can be used as a bactericide target and a key protein of a disease pathogenic mechanism, and has a wide application prospect in prevention and treatment of plant pathogenic fungal diseases.

The invention relates to bacillus subtilis, a biocontrol inoculant prepared from the bacillus subtilis and application of the biocontrol inoculant, and belongs to the technical field of biological inoculants. The bacillus provided by the invention is bacillus subtilis and is named as KC2, the preservation unit is China General Microbiological Culture Collection Center, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, the number of the preservation center is CGMCC No.31372, and the preservation time is July 19, 2024. The bacillus KC2 disclosed by the invention has a certain bacteriostatic effect on different pathogenic bacteria regardless of living bacteria, supernate or cell lysis solution. The bacillus KC2 is high in living ability and wide in application range of nutrient sources, temperature, humidity and pH value. The compound has no pathogenicity to fruits and vegetables, has good tolerance to broad-spectrum bactericides, and has a great application prospect.

The invention belongs to the technical field of modern agriculture, and particularly relates to a biocontrol bacillus amyloliquefaciens BA200 and application thereof. A new biological control bacterium BA200 is separated and identified, is named as bacillus amyloliquefaciens by taxonomy, and has a remarkable inhibition capability on two pathogenic bacteria, namely fusarium oxysporum and phytophthora vigna, causing the cowpea fusarium wilt, and the inhibition rate is up to 60% or above; fermentation liquor prepared from the bacillus amyloliquefaciens BA200 can remarkably reduce the morbidity and disease index of cowpea fusarium wilt, is a good biocontrol microorganism and has application and development potential and important significance in control of cowpea fusarium wilt, and the biocontrol bacterium bacillus amyloliquefaciens BA200 has a broad-spectrum biological control effect, can inhibit various phytopathogens and has a good application prospect. And valuable biocontrol bacterium resources are provided for preparation of biological bactericides.

To provide a novel pest control agent composition, particularly a bactericidal composition.SOLUTION: A bactericidal or bacteriostatic composition comprising an active ingredient A selected from pyrazole compounds or salts thereof represented by formula (I): [G represents G-1; G1 represents C1-C6 haloalkyl or the like; T represents an oxygen atom or the like; RX represents C1-C6 alkyl or the like; RY represents a hydrogen atom or the like; R1 represents a hydrogen atom or the like; R2 represents a hydrogen atom or the like; R3 represents a hydrogen atom or the like; Ra represents a hydrogen atom or the like; Z represents Z-1 or the like; Z1 represents C1-C6 alkyl or the like; m5 and n5 each represent an integer of 0, 1, 2, 3, 4, or 5; and p represents an integer of 0 or 1] and an active ingredient B selected from known bactericidal or bacteriostatic compounds.SELECTED DRAWING: None

The invention relates to the technical field of bactericides, and discloses an oilfield flooding bactericide containing plant bactericidal components and a preparation method thereof. The preparation method comprises the following steps: loading the plant-based traditional Chinese medicine extract and the isothiazolinone-based corrosion inhibitor on the silver-loaded cyclodextrin metal organic framework to prepare an antibacterial agent / silver-loaded cyclodextrin metal organic framework composite material, and mixing the antibacterial agent / silver-loaded cyclodextrin metal organic framework composite material with methyl methacrylate to prepare the antibacterial agent / silver-loaded cyclodextrin metal organic framework composite material. The bactericide obtained by carrying out copolymerization reaction on modified hydroxyquinoline, vinyl quaternary phosphonium salt and methacrylic acid through an in-situ polymerization method can effectively kill and inhibit the breeding of sulfate reducing bacteria and other bacteria in oilfield reinjection water, and has a certain corrosion inhibition effect.

The invention relates to the technical field of reverse osmosis membrane maintenance, in particular to a cleaning scale inhibitor special for a reverse osmosis membrane and application of the cleaning scale inhibitor. The cleaning scale inhibitor provided by the invention comprises an agent A, an agent B and an agent C, the agent A comprises the following components in percentage by mass: 10-20% of organic phosphonic acid, 10-20% of polycarboxylic acid polymer, 5-10% of sulfamic acid, 3-8% of sodium gluconate and the balance of water; the agent B comprises 8-15% of an isothiazolinone bactericide, 5-10% of a nonionic surfactant, 3-8% of a chlorine bactericide, 2-5% of sodium dodecyl benzene sulfonate and the balance of water; and the agent C comprises 10-20% of sodium polyacrylate, 8-15% of sodium hexametaphosphate, 5-10% of citric acid, 3-8% of silicate and the balance of water. The cleaning scale inhibitor provided by the invention has an efficient cleaning effect on various pollutants, is environment-friendly, and does not cause chemical damage to a reverse osmosis membrane.

The invention discloses a novel bradyrhizobium strain SBM1 as well as a preparation and application thereof, and belongs to the technical field of microorganisms. The novel bradyrhizobium strain SBM1 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.29121. The strain has good tolerance to herbicides, bactericides and insecticides, and is high in nodulation and nitrogen fixation capacity, wide in symbiotic spectrum and remarkable in nitrogen-saving and high-yield-promoting capacity. Under the field planting condition that the application amount of nitrogen fertilizer is reduced by 50%, the strain can remarkably improve the nitrogen fixation and growth performance of crops, so that a strain source is provided for obtaining a rhizobium inoculant suitable for planting various varieties of peanuts, the application amount of the nitrogen fertilizer is reduced, normal implementation of crop pesticides is guaranteed, and the economic benefit is increased. The important significance is realized on the increase of the crop yield.

The invention is suitable for the technical field of agricultural bactericides, and provides application of an echinocandin antifungal drug in prevention and treatment of agricultural diseases. According to the present invention, the two common echinocandin antifungal drugs such as the anidulafungin and the Rezafungin acetate, which are common in the field of medicine, are firstly applied to prevention and control of crop diseases; prevention and control tests are carried out on crop fungal diseases such as sclerotiniose, corn southern leaf blight, tomato gray mold, rice blast and crop anthracnose, and results show that the two drugs can significantly inhibit the growth of pathogenic fungal hyphae, and compared with an existing agricultural commercial bactericide carbendazim, the bactericidal composition shows higher inhibitory activity and has the characteristics of high efficiency and low toxicity. The invention lays a foundation for deep research and application of echinocandin drugs in the agricultural field, has a wide market prospect, effectively improves the crop disease prevention and control level, and promotes grain yield increase and quality safety.

The invention discloses an ADF protein from phytophthora as well as a coding gene and application thereof. According to the present invention, the PsADF protein provided by the present invention is the protein represented by sequences 2 and 5 in Phytophthora sojae (Phytophthora sojae); the coding gene is shown as a sequence 3 and a sequence 4. Tests prove that the protein provided by the invention plays an important role in the growth and development process of phytophthora sojae, specifically, after the 64-site mutation of the key amino acid functional site of the protein, the sporangium abnormality and zoospore quantity of the phytophthora sojae are reduced, and the pathogenicity is reduced. The protein provided by the invention has very high application value in control of epidemic disease occurrence and epidemic caused by phytophthora, and provides a molecular target for research and development of novel bactericides in the future. The corresponding ADF inhibitors have different degrees of activity on typical plant pathogenic oomycetes such as phytophthora sojae, phytophthora capsici and phytophthora infestans. The protein provided by the invention has great application potential in the aspect of preventing and controlling the crop epidemic diseases caused by phytophthora plant pathogenic oomycetes, and a novel bactericide developed based on the protein as an action target has important practical significance in controlling the occurrence and prevalence of the crop epidemic diseases caused by phytophthora.

The invention discloses a disinfection tank for wastewater treatment, and belongs to the field of wastewater treatment.The disinfection tank comprises a disinfection tank body, and a liquid inlet device is fixedly installed on one side of the disinfection tank body; the rapid decomposition assembly comprises a heating plate fixedly mounted on the bottom wall of an inner cavity of the disinfection tank, a square rod is rotatably mounted on the bottom wall of the inner cavity of the disinfection tank, a sleeve frame sleeves the outer side of the square rod, a cylinder is rotatably mounted on the top wall of the inner cavity of the disinfection tank, a reciprocating screw rod is arranged in an inner cavity of the cylinder, and a rotating shaft is arranged in the inner cavity of the cylinder; the reciprocating screw rod is rotatably mounted with the disinfection tank and the cylinder, and a movable plate is slidably mounted in an inner cavity of the cylinder; according to the sterilizing tank, wastewater and a sterilizing agent in the sterilizing tank can be uniformly heated, and the process of decomposing the sterilizing agent into active ingredients can be accelerated by combining heating with efficient mixing, so that the residual sterilizing agent which is not completely reacted and the generation of potential pollutants are reduced, and the sterilizing efficiency is further improved.

The invention provides a cleaning agent with double effects of cleaning and sterilization for a scrubber and a preparation method of the cleaning agent. The cleaning agent comprises the following components in parts by weight: 5-10 parts of a solvent, 10-20 parts of a surfactant, 0.5-3 parts of a washing assistant, 0-1 part of a pH regulator, 1.5-3 parts of a bactericide, 0.3 part of essence, 0.01-0.1 part of an organic silicon emulsion and the balance of deionized water, wherein the total parts by weight of the components are 100. According to the invention, low-foam and foam-inhibiting components and long-acting antibacterial components are added, and the surfactant matched with the system and the addition amount of the surfactant are adjusted at the same time, so that while the cleaning and decontamination capabilities are ensured, the cleaning agent is endowed with strong bactericidal capability and long-acting antibacterial performance, the interior and exterior of the scrubber equipment are not damaged, and the occurrence of the situation that the equipment is mistakenly reported and halted is avoided.

The invention relates to the field of biology, in particular to phytophthora sojae psFBP21 protein, a psFBP21 gene, a biological material and application thereof. The amino acid sequence of the phytophthora sojae psFBP21 protein provided by the invention is as shown in SEQ ID NO. 2; the nucleotide sequence of the coding gene is as shown in SEQ ID NO. 1. Experiments prove that the phytophthora sojae psFBP21 protein provided by the invention plays an important role in the growth and development process of phytophthora sojae, specifically, after the protein is deleted, the growth of phytophthora sojae hyphae is slowed down, the number of oospore is reduced, the pathogenicity is reduced and the like. The conclusion provides a technical basis for exploring phytophthora sojae development and a pathogenic molecular mechanism, and provides a molecular target for research and development of novel bactericides in the future.

The invention discloses bacillus subtilis LXN-1, and the bacillus subtilis LXN-1 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC.NO 31697. The bacillus subtilis LXN-1 is a bacillus subtilis LXN-1 strain. The bacillus subtilis LXN-1 has an inhibition effect on various plant pathogenic bacteria, the inhibition rate on tomato botrytis cinerea can reach 61.5%, the inhibition rate on tobacco brown spot caused by alternaria longibrachiata can reach 72%, the bacillus subtilis LXN-1 also has a virus resistance effect, the inhibition rate on tobacco TMV viral plaque can reach 95%, and the bacillus subtilis LXN-1 has a good application prospect. In addition, the bacillus subtilis LXN-1 also has the potential of being compounded with pesticide bactericides with iprodione, trifloxystrobin, difenoconazole, pyraclostrobin, copper oxychloride and copper hydroxide as main components for use, and has market popularization and application values, and absorption of the plants to nutrient elements can be enhanced, and the growth of the plants can be promoted, and the bacillus subtilis LXN-1 also has the potential of being compounded with the pesticide bactericides with the iprodione, the trifloxystrobin, the difenoconazole, the pyraclostrobin, the copper oxychloride and the copper hydroxide as main components.

The invention discloses a method for detecting drug resistance of botrytis cinerea to succinate dehydrogenase (SDHI) bactericides. On the basis of an RPA-CRISPR / Cas12a system, a specific primer pair amplification target fragment and crRNA for activating Cas12a trans-cleavage activity are designed aiming at key mutation sites related to drug resistance in a bactericide action target SdhB gene, so that fluorescent light visible to naked eyes can be generated only when a resistant strain sequence is matched with the crRNA, and the fluorescent light is opposite to the fluorescent light generated when a sensitive strain sequence is matched with the crRNA. The method provided by the invention overcomes the dependence on laboratories and large instruments in the traditional drug resistance detection method, provides key technical support for accurate prevention and control and drug resistance management of grape gray mold, and provides direct drug use basis for scientific prevention and control in the field.

The invention relates to a seawater-based delayed cross-linking agent, the seawater-based delayed cross-linking agent is a poly (ammonia boron) cross-linking agent, and the poly (ammonia boron) cross-linking agent is one of a poly (ammonia boron) cross-linking agent I and a poly (ammonia boron) cross-linking agent II; the seawater-based ultra-low-concentration guanidine gum fracturing fluid is prepared from the following components in percentage by mass: 0.20 to 0.35 percent of a vegetable gum thickening agent, 0.20 to 0.40 percent of a seawater-based delayed cross-linking agent, 0.10 to 0.30 percent of a seawater-based cross-linking synergist, 0.05 to 0.10 percent of a pH (Potential of Hydrogen) regulator, 0.05 to 0.10 percent of a cleanup additive, 0.2 to 3 percent of an anti-swelling agent, 0.03 to 0.20 percent of a sterilizing agent and the balance of seawater, according to the invention, the water cost of fresh water can be saved, the transportation cost of transporting fresh water can also be saved, and the effect of saving the cost of fracturing fluid is especially remarkable.

The invention belongs to the technical field of disease control, and discloses a bactericide for controlling radix bupleuri root rot, which is prepared from the following components: 10%-20% of a vesicle-arbuscular mycorrhizal agent, 30%-50% of carbendazim, 15%-25% of JS399-19, 10%-20% of phenazino-1-carboxylic acid, 10%-20% of bacillus subtilis, 3%-7% of a surfactant, 3%-7% of a stabilizer and the balance of a carrier, the dispersing agent is used for diluting and dispersing the active ingredients. By scientifically proportioning various efficient active components such as the vesicle-arbuscular mycorrhizal agent, the carbendazim, the phenamacril, the phenazino-1-carboxylic acid and the bacillus subtilis, a set of multi-target and multi-layer disease prevention and control system is formed, pathogenic bacteria of radix bupleuri root rot are accurately struck, growth and reproduction of radix bupleuri root rot are effectively inhibited, and the disease incidence rate is greatly reduced. Compared with a traditional medicament, the bactericide not only has a remarkable control effect, but also is safe and friendly to human bodies, animals and the environment, the use frequency and the total amount of the medicament are reduced, the requirements of consumers on green agricultural products are met, and occurrence and spreading of radix bupleuri root rot are controlled.

The invention belongs to the technical field of agricultural microorganisms, and discloses a bacillus flexus strain BJ699 and application thereof in strawberry production. Specifically, the invention provides a novel bacillus flexus strain BJ699, and the preservation number of the strain BJ699 in the China Center for Type Culture Collection is CCTCC (China Center for Type Culture Collection) NO: M20252013. The invention also provides a microbial agent containing the strain BJ699 as an active component and application of the strain BJ699 in preparation of a microbial agent for prevention and control of strawberry root rot pathogenic bacteria and / or degradation of pyraclostrobin. The new strain or the microbial agent containing the strain comes from the natural environment, has no cross resistance to existing bactericides and insecticides, has the advantages of high efficiency, low toxicity, no environmental pollution, low possibility of drug resistance generation and the like, and has a wide application prospect.

The invention relates to the technical field of sewage treatment, in particular to a preparation method of a degradable bactericide for an oil field. According to the invention, a hydroxypropyl guanidine gum-polycaprolactone-ethylenediamine fragment copolymer is taken as a carrier, a methylisothiazolinone quaternary ammonium salt (Q-MIT) bactericide is loaded, and the degradable bactericide for the oil field is obtained by cooperating with a dodecyl ethyl sulfide (TC-SE) bactericide and combining with sodium polyaspartate. A carrier forms a hydrogen bond with a polar group on the surface of a biological membrane through a hydroxypropyl guanidine gum main chain to realize targeted anchoring, a polycaprolactone branched chain encapsulates Q-MIT through a hydrophobic effect, primary amino groups of ethylenediamine are introduced to form a pH sensitive Schiff base bond with Q-MIT, and the primary amino groups and adipic dihydrazide form a thermal response hydrazide bond to construct a dual dynamic covalent bond, so that controlled release of Q-MIT is realized, and the biomembrane is prepared. And moreover, the components of the whole system are connected through degradable chemical bonds, so that the environmental protection property and sustainability of oil field operation are guaranteed.

The invention relates to a synergistic composition for preventing and treating celery root rot. The tannic acid and fludioxonil in the synergistic composition disclosed by the invention show a relatively good synergistic effect in a compounding mass ratio range of (1: 50)-(50: 1). According to the synergistic composition disclosed by the invention, tannic acid with weak bacteriostatic activity on celery root rot and fludioxonil with excellent bacteriostatic activity are compounded for use, so that a remarkable synergistic effect is achieved, the prevention and treatment effect is far higher than that of a single agent, and the synergistic effect is achieved by reducing pesticide. Besides, according to the synergistic composition disclosed by the invention, the plant source tannic acid and the chemical bactericide fludioxonil are compounded for use, and the use amount of chemical agents is reduced, so that the resistance risk of the chemical bactericide is reduced, pesticide residues and ecological environment pollution are reduced, and the synergistic composition has a good application prospect.

To provide a bactericidal agent for penetration into the deep layer of dental plaque that allows a bactericidal component to penetrate from the surface layer to the inner deep layer of dental plaque.SOLUTION: Provided is a bactericidal agent for penetration into the deep layer of dental plaque that comprises glycyrrhetinic acid (X) and a cationic bactericidal agent (Y) as active ingredients.SELECTED DRAWING: None

The invention relates to the technical field of gastrodia elata cultivation, in particular to a fungus material for gastrodia elata cultivation and a cultivation method.The fungus material contains plants capable of inhibiting pathogenic bacteria causing gastrodia elata tuber rot disease, and the fungus material is composed of, by mass, 58% of sawdust, 30% of cottonseed hulls, 10% of maytenus stems, 1% of gypsum powder, 0.5% of KH2PO4 and 0.5% of MgSO4. The fungus material provided by the invention contains the plant maytenus stems capable of inhibiting pathogenic bacteria causing the gastrodia elata tuber rot disease, the ethanol extract of the maytenus stems can efficiently inhibit the pathogenic bacteria primary verticillium atrophaeum causing the gastrodia elata tuber rot disease, and in addition, the ethanol extract of the maytenus stems has no obvious inhibiting effect on armillaria mellea; the method ensures that a gastrodia elata-armillaria mellea symbiotic system is not interfered, the field experiment control effect is 76.85% when the fungus material is used and a method for spraying the maytenus stem ethanol extract bactericide is combined, and the method has important value for ensuring sustainable development of the gastrodia elata industry.

According to the suspending agent containing the pydiflumetofen and the meconazole, provided by the invention, the defects of a traditional single bactericide are overcome, and the control effect on various plant diseases is improved. According to the suspending agent disclosed by the invention, a natural biological source substance chitosan oligosaccharide is introduced as a thickening agent, so that the prevention and treatment effect of the agent on the rice sheath blight disease can be improved unexpectedly.

The invention relates to a sterilization liquid agent based on cuprous hydroxide, and also relates to a preparation method and a use method thereof, the suspension of cuprous hydroxide is used as the sterilization liquid agent, and diseases caused by various oomycetes, fungi and / or bacteria can be effectively prevented and treated; wherein the turbid liquid of cuprous hydroxide is obtained by stirring a cuprous chloride coordination compound in a solution state and decomposing the cuprous chloride coordination compound in water. According to the sterilization liquid, the single-time copper dosage is far lower than that of an existing copper ion sterilization agent, and meanwhile the prevention and treatment range of diseases is wider. Therefore, the risk of excessive heavy metal residue caused by the existing copper preparation is solved, and the problem that multiple bactericides need to be used at the same time for preventing and treating one or more diseases is also avoided. The bactericidal liquid provided by the invention has important significance for preventing and treating plant diseases, and has huge application potential in the agricultural market.

The invention relates to the technical field of agricultural plant disease control, and particularly discloses a biological and chemical compound bactericide and application thereof. The biological and chemical compound bactericide comprises a bacterial solution of a biocontrol strain bacillus velezensis LHN1 and a chemical bactericide, the biocontrol strain bacillus velezensis LHN1 is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 63550; the chemical bactericide is selected from at least one of prochloraz, pyraclostrobin or tebuconazole; in the compound bactericide, the volume ratio of the LHN1 bacterial liquid to the chemical bactericide is (1: 4)-(4: 1). Through the synergistic effect of biological prevention and control and chemical prevention and control, the problems that a single chemical bactericide pollutes the environment and the single biological prevention and control effect is unstable are solved, the prevention effect in a pot experiment reaches up to 83.99%, meanwhile, the dosage of chemical agents is reduced by 30%-50%, and the method has the advantages of being efficient, low in toxicity and low in cost and is suitable for root rot prevention and control in large-scale planting of rhizoma polygonati.

The invention belongs to the field of pesticide preparations, and particularly relates to a bactericidal composition containing bixafen and application of the bactericidal composition. The bixafen bactericidal composition comprises the following components in percentage by weight: 3-10% of bixafen, 5-20% of triazole bactericide, 8-12% of Termul 5030, 1-3% of Atlox 4912, 1-5% of ethylene glycol, 13-18% of cyclohexanone and the balance of deionized water, wherein the total percentage is 100%. And the triazole bactericide is tebuconazole or prothioconazole. The microemulsion prepared by the invention has excellent preparation performance, and the emulsifier is compounded, so that compact micelles can be formed, the solubilizing amount can be increased, the dosage of the emulsifier can be reduced, the production cost can be reduced, and the microemulsion has an excellent prevention and treatment effect on wheat scab.

The invention relates to the technical field of pesticides, in particular to a hydrazide derivative containing a 3, 4-dihydroisoquinoline structure, a preparation method and application. The hydrazide derivative containing the 3, 4-dihydroisoquinoline structure is a compound as shown in a formula (I), or a stereoisomer, a tautomer, an isotope derivative and a pesticide acceptable salt thereof. The hydrazide derivative containing the 3, 4-dihydroisoquinoline structure has broad-spectrum inhibitory activity on phytopathogens such as plant pathogenic fungi, especially has excellent inhibitory activity on basidiomycota and ascomycomycota pathogenic fungi such as gaeumannomyces graminis, physalospora piricola, sclerotinia sclerotiorum, botrytis cinerea and rhizoctonia solani, and has broad-spectrum inhibitory activity. The bactericide is expected to be developed into a novel green bactericide which is efficient, safe, economical and environment-friendly.