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112 results about "Zoospore" patented technology
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A zoospore is a motile asexual spore that uses a flagellum for locomotion. Also called a swarm spore, these spores are created by some protists, bacteria and fungi to propagate themselves.
Method for generating sporangia and releasing zoospore by inducing phytophthora capsici
ActiveCN105441375AShort induction periodNo pollution in the processMicroorganism based processesSpore processesContinuous lightSporangium
The invention discloses a method for generating sporangia and releasing zoospore by inducing phytophthora capsici. The method includes the steps that phytophthora capsici is activated, an inducing solution for generating sporangia is prepared, sporangia are induced to be generated, and zoospore is induced to be released. The inducing solution used in the method is originally imported V8 vegetable juice with the volume concentration being 10-15% and a CaCO3 solution with the volume concentration being 0.2-0.3 g / L. When phytophthora capsici is induced to generate the sporangia, continuous light culture is carried out under a white fluorescent lamp. The method is simple, convenient to use, quick, free of pollution and capable of being used in phytophthora capsici pathogenicity measurement and biological activity measurement, achieved through an antibacterial agent, of phytophthora capsici.
Owner:INST OF PLANT PROTECTION FAAS
Method for constructing manually controlled submarine algae field
InactiveCN102138513AAchieve autonomous operationLow costClimate change adaptationCultivating equipmentsOcean bottomEmbryo
The invention provides a method for constructing a manually controlled submarine algae field. The method comprises the steps of selection and treatment of seaweed substrates, culture of seedlings of seaweeds, sowing and the like, wherein the seaweed substrates are breeding screens formed by weaving reddish brown ropes; the step of culture of seedlings of seaweeds comprises the substeps of collection of seedlings of seaweeds and management of culture of seedlings; after the water density of the collected spores during release in the substep of collection of seedlings of seaweeds reaches 10-15 zoospores in the field of 100-times microscope, the seaweed substrates are put in the seawater to adhere; and the adhesion density is 20-30 embryo spores in the field of 100-times microscope. The method has the following advantages and beneficial effects: the variety, density, place and sowing time of the submarine algae field achieve manual control; the fixed substances of the algae, such as C, N, P and the like, are taken from the sea to the land by harvesting the seaweed, so the effect on solving the ecological problems is longer; the cost is far lower than that of the artificial reefs; thenatural submarine geomorphology is not damaged; and the submarine algae field can be formed in a shorter time, thus rapidly increasing the resource quantity.
Owner:QINGDAO AGRI UNIV
Single spore isolation method of phytophthora capsici zoospores
InactiveCN105886451AAvoid easy separationEasy to operateFungiMicroorganism based processesSterile waterAntibiotic Y
The invention relates to a single spore isolation method of phytophthora capsici zoospores. The single spore isolation method comprises the steps that phytophthora capsici is inoculated to a V8 culture medium, sealing is performed with a sealing membrane, culture at the constant temperature of 25 DEG C is performed for 5 days, then the sealing membrane is removed, irradiation is performed for 24 hours, sterile water of 4 DEG C is added to perform induction and constant-temperature release respectively for 25 minutes so as to obtain a zoospore suspension, the suspension is diluted to reach the standard that 1-2 spores exist in each (10*) view, 1 mL of the suspension is taken and added to a V8 culture medium flat plate containing antibiotics in an evenly coated mode, constant-temperature standing is performed for 6 hours, a single germinated zoospore is found through an inverted microscope and is cut by using a scalpel to form a 0.5 cm square mark, it is confirmed that single spores are transferred to a flat antibiotic plate, and purified spores are single spore plants. The single spore isolation method is simple and convenient to operate, high in accuracy and good in rapid separating effect, adopts simple devices and can separate single spores of a large number of phytophthora capsici zoospores within a short time.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY
Method of inducing phytophthora nicotianae breda de haan bacteria to generate sporangiums and release zoospores
ActiveCN103045529AShort induction periodNo pollution in the processSpore processesSnow moldNicotiana tabacum
The invention belongs to the technical field of plant protection, and discloses a method of inducing phytophthora nicotianae breda de haan bacteria to generate sporangiums and release zoospores. The method comprises the following steps: activating the phytophthora nicotianae breda de haan bacteria, preparing the induction liquid generating sporangiums and inducing to generate sporangiums and release zoospores. According to the invention, the used induction liquid is the Bache wild vegetable composite fruit and vegetable juice with the volume concentration of 15-25 percent and the compound fertilizer solution with the mass concentration of 0.05-0.20 percent, and when the phytophthora nicotianae breda de haan is induced to generate the sporangiums, the continuous illumination culture is performed under the white fluorescent lamp. The method provided by the invention is simple, convenient, rapid and pollution-free, and can be used for the researches on virulence determination of the phytophthora nicotianae breda de haan bacteria, the biological activity assay of the phytophthora nicotianae breda de haan bacteria by the sterilizing agent, the evaluation of disease resistance of an epidemic disease by the luffa varieties and the like.
Owner:PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
Molecule detection technique of cayenne pepper phytophthora capsici pectin lyase (Pcpel) 1 genes
InactiveCN101643781AMicrobiological testing/measurementMicroorganism based processesCayenne pepperPhytophthora sp.
The invention relates to a molecule detection technique of cayenne pepper phytophthora capsici pectin lyase (Pcpel) 1 genes, belonging to the field of molecular biology. A pair of specific primers aredesigned and synthesized by the cayenne (sweet) pepper phytophthora capsici pectin lyase (Pcpel) 1 genes, the specificity of a plurality of tested bacteria species is verified by PCR, and then the sensitivity of the specific primers is verified by carrying out quantitative molecule detection for a soil cayenne pepper phytophthora oospore and a zoospore; and on the basis, the qualitative and quantitative molecule detection and the early warning of cayenne pepper phytophthora capsici contained in cayenne pepper diseased field soil, diseased stems, diseased leaves and diseased fruits are carriedout. The invention can detect and early warn the cayenne pepper phytophthora capsici in different periods and pathopoiesia characters thereof, thereby being convenient to establish a comprehensive prevention and control technology strategy, determining and selecting an optimal prevention and control period to carry out comprehensive prevention and control and high-efficiency management for the cayenne pepper phytophthora capsici, really controlling the infection and the harm of pathogenic bacteria from a source and enhancing the comprehensive prevention and control effect of the cayenne (sweet) pepper phytophthora capsici.
Owner:SHANDONG AGRICULTURAL UNIVERSITY
Seedling cultivation method for sea-tangle
InactiveCN101502232AGuaranteed healthy growthImprove simplicityCultivating equipmentsSeaweed cultivationSporophyteIrradiation
The present invention discloses a seedling cultivation method of sea tangle, wherein the method comprises the following steps: a. cultivating the collected zoospores under the white radiation irradiation with a certain illumination intensity for forming gametophyte; b. cultivating the gametophyte with the blue light illumination with a certain illumination intensity for converting the gametophyte to micro sporinite; c. growing the micro sporinite under the red light illumination with a certain illumination intensity for a certain period of time; and d. cultivating the sporinite under blue light illumination with a certain illumination intensity so that the sporinite obtains the dimension required for stored cargo delivery. The healthy growing of sporinite can be guaranteed under a higher temperature through adopting the combination irradiation with various light qualities for executing seedling cultivation of sea tangle. The method is simple for applying and the seedling cultivation cost is low.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Loop-mediated isothermal amplification (LAMP) primers for detecting Phytophthora capsici and kit comprising primers
InactiveCN102586469AAccurate detectionExempt investmentMicrobiological testing/measurementMicroorganism based processesDiseased plantPcr method
The invention provides loop-mediated isothermal amplification (LAMP) primers for detecting Phytophthora capsici and a kit comprising the primers. The primers comprise FIP, BIP, F3 and B3 (shown as Seq ID No.1-4). An LAMP primer technology is used for quickly detecting the Phytophthora capsici, and the Phytophthora capsici can be accurately detected in a complicated pathogenic bacteria environmentin diseased plant tissues and soil. The method has higher specificity and sensitivity than the conventional PCR method, propagules of the Phytophthora capsici in various forms, such as mycelia, oospores and zoospores can be detected, and the method has significance in aspects of early warning of Phytophthora capsici epidemics and monitoring pathogeny in epidemic areas; meanwhile, expensive instruments can be avoided, and the method is easily promoted and used in basic level.
Owner:INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
Method for inducing phytophthora parasitica var. nicotianae to generate zoosporangium and release spore
ActiveCN102226167AEfficient productionProduce short and focusedMicroorganism based processesSpore processesNicotiana tabacumMycelium
The invention discloses a method for inducing phytophthora parasitica var. nicotianae to generate zoosporangia and release spores. The method comprises steps of: culturing phytophthora parasitica var. nicotianae mycelia on an oat medium prepared in a narrow mouth conical flask; preparing an induction liquid for generation of zoosporangia by selecting roots of tobacco seedlings, with 6-7 pieces of true leaves completely expanding, of a black shank infected variety; inducing the mycelia with the induction liquid to generate zoosporangia and to release spores. According to the invention, a characteristic that a black shank infected tobacco variety is more easily to generate affinity reaction with phytophthora parasitica var. nicotianae is employed, and a filtrate of seedling roots of the black shank infected tobacco variety is used as the induction liquid, so as to induce the cultured mycelia to generate zoosporangia. Proved by tests, the invention has a short induction time, high zoospore release rate, high obtained zoospore content; and scraping mycelia for culture is not needed. Therefore, the invention is of less pollution and easy to operate.
Owner:YUNNAN ACAD OF TOBACCO AGRI SCI
Biocontrol strain BCJB01 and application thereof
The invention discloses a biocontrol strain BCJB01 and application thereof. Bacillus cereus BCJB01 is preserved in China General Microbiological Culture Collection Center on January 30, 2015 with the preservation number to be CGMCC NO. 10477. The Bacillus cereus BCJB01 has the unique action of decomposing downy mildew zoospores, and thus having good controlling effect to both grape downy mildew and cucumber downy mildew.
Owner:BIOLOGY INST OF SHANDONG ACAD OF SCI +2
Phytophthora capsici effector RxLR19781 gene and application thereof
The invention provides a phytophthora capsici effector RxLR19781 gene and application thereof. By using multiple genetic engineering techniques, biological functions of the phytophthora capsici effector RxLR19781 (SEQ ID NO:2) gene and an interactions protein GME (guanosine diphosphatemannose mannose 3'5' epimerase (SEQ ID NO:4) are revealed, GME is a key factor in the process of regulating and controlling RxLR19781 to control zoospore infestation functions, RxLR19781 is capable of inhibiting necrosis caused by Bax, and results that RxLR19781 is further silenced in phytophthora capsici show that pathogenicity of the phytophthora capsici can be weakened by RxLR19781.
Owner:SHANDONG AGRICULTURAL UNIVERSITY
Molecule detection technique of cayenne pepper phytophthora capsici polygalacturonase (Pcipg) 5 genes
InactiveCN101643782AReduce the cost of prevention and controlProving testabilityMicrobiological testing/measurementMicroorganism based processesCayenne pepperPentagalacturonic acid
The invention relates to a molecule detection technique of cayenne pepper phytophthora capsici polygalacturonase (Pcipg) 5 genes, belonging to the field of molecular biology. A pair of specific primers are designed and synthesized by the cayenne (sweet) pepper phytophthora capsici polygalacturonase (Pcipg) 5 genes, the specificity of a plurality of tested bacteria species is verified by PCR, and then the sensitivity of the specific primers is verified by carrying out quantitative molecule detection for a soil cayenne pepper phytophthora oospore and a zoospore; and on the basis, the qualitativeand quantitative molecule detection and the early warning of cayenne pepper phytophthora capsici contained in cayenne pepper diseased field soil, diseased stems, diseased leaves and diseased fruits are carried out. The invention can detect and early warn the cayenne pepper phytophthora capsici in different periods and pathopoiesia characters thereof, thereby being convenient to establish a comprehensive prevention and control technology strategy, determining and selecting an optimal prevention and control period to carry out comprehensive prevention and control and high-efficiency managementfor the cayenne pepper phytophthora capsici, really controlling the infection and the harm of pathogenic bacteria from a source and enhancing the comprehensive prevention and control effect of the cayenne (sweet) pepper phytophthora capsici.
Owner:SHANDONG AGRICULTURAL UNIVERSITY
Primer for detecting the soybean phytophthora and kit and method thereof
InactiveCN101805796AAccurate diagnosisAccurate detectionMicrobiological testing/measurementDNA/RNA fragmentationPropagulePhytophthora sp.
The invention provides a primer for detecting the soybean phytophthora, and a kit and a method thereof. The primer and the detecting method for detecting the soybean phytophthora have high accuracy, high specificity and high sensitivity, and can detect the various propagules of the soybean phytophthora such as hypha, oospore, zoospore and the like. The whole technique is fast, sensitive and accurate, which has important significance in the prevention of foreign soybean phytophthora, the detection of pathogenic bacteria in domestic affected area, the international trade and the like.
Owner:NANJING AGRICULTURAL UNIVERSITY
Method for phytophthora nicotianae inoculation of tobacco
InactiveCN104396599APrecise screeningEfficient screeningHorticulture methodsSporelingNicotiana tabacum
The invention discloses a method for phytophthora nicotianae inoculation of tobacco. The method for the phytophthora nicotianae inoculation of tobacco includes steps that (1) preparing for tobacco seed plantlets; (2) preparing phytophthora nicotianae zoospore suspension; (3) irrigating the roots to inoculate; (4) counting the morbidity situation, and evaluating the disease resistant result. The zoospore inoculated to the roots of the seed plantlets can be quantified rigidly, the resistant difference between the same variety due to excessive inoculation amount is avoided, and the resistance identification is more precise and effective; in the actual operation, the inoculation can be performed in quantities, the space usage is small, the labor cost is saved, and the experiment period is shortened. The method for the phytophthora nicotianae inoculation of tobacco is suitable for popularization.
Owner:YUNNAN ACAD OF TOBACCO AGRI SCI
Kelp germplasm separation method
ActiveCN103266079AMeet the needs of rapid growthAvoid affecting water qualityPlant cellsWater qualityGermplasm
The invention relates to a kelp germplasm separation method. According to the kelp germplasm separation method, when kelp zoospores are diffused, kelp is inside and outside wrapped by medicine, gauze and bolting-silk, so that kelp mucus and impurities can be effectively prevented from influencing water quality; a pipettor separation gametophyte can be used for effectively improving the separation efficiency and avoiding pollution; and a culture medium is improved, so that the rapid growth requirement of the kelp zoospores is satisfied, and the pollution can be effectively avoided. Therefore, the kelp germplasm separation method is safe and high-efficiency.
Owner:MARICULTURE INST OF SHANDONG PROVINCE
High-efficiency biocontrol strain screening method taking pepper phytophthora blight as target
InactiveCN103981249ALow priceEasy to buyMicrobiological testing/measurementMicroorganism based processesBiotechnologyPhytophthora sp.
Owner:텐진인스티튜트오브플랜트프로텍션
Method for collecting and cultivating offspring seed of wild monostroma nitidum
InactiveCN101611693AIncrease productionIncrease chlorophyll contentClimate change adaptationCultivating equipmentsSlow growthThallus
The invention disclosed a method for collecting and cultivating offspring seed of wild monostroma nitidum. The method comprises the following steps: building an appropriate seed bed and adopting an appropriate seed net to ensure that zoospores of offspring seed of wild monostroma nitidum are attached to ropes of the seed net for growing into thallus, so as to build appropriate living and growing space for wild monostroma nitidum; then managing the seed bed to ensure high yield of the wild monostroma nitidum on the seed bed and high content of chlorophyll of the wild monostroma nitidum and less sediment containing of gained wild monostroma nitidum, thereby improving the quality of the wild monostroma nitidum; the method solves the defects of long seed raising period in a man-made chamber, slow growth, higher input cost, high management difficulty and the like; the method can be applied to large scale production, the seed bed can be repeatedly used, the production cost is low, the thallus on the seed net can be easily collected to reduce the waste of mudflat and reef collection; in addition, the obtained monostroma nitidum is natural wild monostroma nitidum and is ideal green food.
Owner:NINGBO UNIV
Culture medium for prompting Phytophthora capsici Leonian to generate zoosporangium
InactiveCN104726390AInduced generationInduce and induceFungiMicroorganism based processesPathogenicityZoospore
The invention particularly discloses a culture medium for prompting Phytophthora capsici Leonian to generate zoosporangium, belonging to the technical field of culture mediums. The culture medium is prepared from the steps of slicing 200g of peeled carrots into pieces, boiling in 1500ml of boiling water for 30 minutes, filtering, extracting filtrate, adding water into the filtrate until the solution is 1000ml, adding 15g of agar, uniformly stirring, carrying out sterilization at 121 DEG C for 15 minutes, so as to obtain the culture medium for prompting Phytophthora capsici Leonian to generate zoosporangium. The culture medium is prepared from yellow carrots and is capable of effectively inducing and prompting the Phytophthora capsici Leonian to generate zoosporangium, so that the problem that zoosporangium is very difficulty generated from Phytophthora capsici Leonian strains P2 and P3 on a red carrot culture medium is solved, and the inoculation requirement of a lots of zoospores can be effectively met, thereby being favorable to the quantitative research of researchers on pathogenicity of these strains.
Owner:INNER MONGOLIA AGRICULTURAL UNIVERSITY +1
PCR (Polymerase Chain Reaction) detection primer for phytophthora capsici leonian, kit containing primer and application thereof
InactiveCN102643903AQuick checkAccurate detectionMicrobiological testing/measurementDNA/RNA fragmentationPropagulePhytophthora sp.
The invention provides a PCR (Polymerase Chain Reaction) detection primer for phytophthora capsici leonian. The primer comprises Enl4s and Enl1a (as shown in Seq ID No.1 and 2). Four pairs of specific primers capable of quickly detecting phytophthora capsici leonian germs can be obtained based on the sequence difference between the Enl and Ypt genes of the phytophthora capsici leonian and other phytophthora nicotianaes: Enl2s / Enl3a, Enl4s / Enl1a, Ypt2s / Ypt3a and Ypt2s / Ypt5a, wherein the sensitivity of Enl4s / Enl1a is the highest. A PCR detection system is established on the basis, which is capable of quickly and accurately detecting the phytophthora capsici leonian from complex pathogenic bacteria environments in the tissues of plants having diseases and soil. A detection kit established according to the method is simple and convenient for operation, good in specificity and high in sensitivity; and the detection kit is capable of detecting the propagules in various forms of the phytophthora capsici leonian, such as hypha, oospore, zoospore and the like, thereby having great significance in the aspects of early warning on the epidemic situation of the phytophthora capsici leonian, pathogen supervision on the epidemic area and the like.
Owner:INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
Screening method of biocontrol bacteria and dosage for tobacco black shank
InactiveCN102277408AAvoid missingAvoid screeningMicrobiological testing/measurementMicroorganism based processesBiotechnologyNicotiana tabacum
The invention discloses a method for screening biocontrol bacteria and dosage thereof against tobacco black shank. The method comprises the following steps: a. cultivating sterile tobacco seedlings on filter paper in a petri dish; b. on the basis of step a , add bacterial culture solution with biocontrol potential in the petri dish; c, after the tobacco seedlings and the bacterial suspension with biocontrol potential are cultivated together for 24 hours, add the zoospore suspension of tobacco black shank bacteria to the petri dish d, on the basis of step c, give the tobacco seedlings normal growth conditions, detect the control effect of the bacteria with biocontrol potential added by observing the morbidity of the tobacco seedlings after 7 days, and screen out the biocontrol that can prevent and treat tobacco black shank bacteria. Compared with the traditional method, the present invention has many advantages. It is simple, fast, less workload, high efficiency, accurate and reliable screening result, and can screen bacteria with bio-control function from a large number of unknown bacteria in a short period of time.
Owner:GUIZHOU TOBACCO SCI INST
Molecular detection primer specific for phytophthora sojae and application thereof
ActiveCN103571946AMicrobiological testing/measurementFluorescence/phosphorescenceSporelingPhytophthora sojae
The invention designs a molecular detection primer special for phytophthora sojae, which adopts R-SNARE protein coding gene Ykt6 needed by cell survival as a target. The primer has high sensitivity and is matched with a universal primer Ykt6F / Ykt6R of Ykt6 phytophthora; a nested PCR method is adopted, so that the sensitivity can be improved by 1000 times and trace phytophthora sojae can be detected; according to the method, specificity and sensitivity are high and hypha, oospore and zoospore of the phytophthora sojae can be detected. Simultaneously, the special for the phytophthora sojae is utilized for developing the detection method for real-time quantitative PCR and a linear regression equation, the amount of infected phytophthora sojae in soybean can be accurately and quantitatively detected and the content of the oospore of the residual phytophthora sojae in the soil can be detected so as to provide forceful data for prediction and forecast of diseases and provide technical support for entry and exit quarantine.
Owner:PLANT PROTECTION & QUALITY & SAFETY OF AGRI PRODS INST ANHUI ACAD OF AGRI SCI
Porphyra suspended filamentous form cell ripening accelerating device and method
InactiveCN1673342ADispersion is obviousSimple structureTissue/virus culture apparatusPorphyraEngineering
The present invention discloses one kind of suspending laver filiform cell mature promoting apparatus and method. In proper zoospore collecting season, the suspension cultured cells may be made to mature in great amount to produce conchospores with normal germination function for collecting. The apparatus consists of four parts including flushing bag, overflow barrel, water pump and water returning channel. After nearly mature laver filiform cell in 150-450 g is set inside the flushing bag, the water pump is made to run for 2-4 nights so that water inside the bag is rolled to make the cell become mature. The present invention can meet the requirement of raising laver spore.
Owner:INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Method for identifying resistance of epidemic diseases of capsicum annuum at seedling stage by root-dipping and inoculating
PendingCN110628870AReduce demandTime-consuming and labor-intensive solutionMicrobiological testing/measurementDiseaseProblem of time
The invention belongs to the technical field of disease resistance identification of horticultural crops and particularly relates to a method for identifying the resistance of epidemic diseases of capsicum annuum at the seedling stage by root dipping and inoculating. The method comprises the steps of capsicum annuum seedling preparation, spore suspension preparation, inoculation, disease investigation, evaluation and the like, wherein the bared root and caudex parts of the capsicum annuum seedlings are soaked with the spore suspension having a concentration of 10 zoospores / mL when the seedlingage is 5-6-leaf stage; the disease index is investigated on the fifth day after inoculation, and grading is performed according to the disease index, and the resistance of varieties is evaluated. Themethod can identify the resistance by utilizing a root-dipping and inoculating method, has the advantage of low inoculation concentration, can solve the problems of time and labor consumption for culturing mass bacteria, is in accordance with natural incidence situation since bacteria contact with the root part, is standard and scientific in disease index grading, has low requirement for the inoculation environment, is easy to use, and provides technical support to screening of large-scale capsicum annuum disease-resistant breeding materials.
Owner:黑龙江省农业科学院园艺分院
Bacillus for preventing and treating root rot of paris polyphylla, and preparation method and application thereof
The invention discloses bacillus for preventing and treating root rot of paris polyphylla, and a preparation method and application thereof. A paenibacillus polymyxa strain HJ-2 is adopted to infect the fusarium zoospores, then germination of fusarium spore is further inhibited, and harm of fusarium to rhizome parts of the paris polyphylla is reduced, so that harm of root rot to the paris polyphylla is reduced, the yield of the paris polyphylla is ensured, and economic loss of pesticide farmers is reduced. In addition, the paenibacillus polymyxa strain HJ-2 is used for biological prevention, so that pesticide residues are not generated, and the bacillus provided by the invention has the characteristics of greenness and recyclability, good prevention and control effect, avoidance of pesticide residue pollution and the like.
Owner:汉江师范学院
Method for vaccination soybean with phytophthora sojae
InactiveCN101268744AEasy to handleSmall footprintHorticulture methodsPlant genotype modificationAgricultural sciencePhytophthora sojae
The invention discloses a method for Phytophthora sojae to inoculate sojae. The method for Phytophthora sojae to inoculate sojae provided by the invention is a method in which the spore suspension of the Phytophthora sojae is used to inoculate a sojae seedling of which the radicel exposes out of the seed capsule. A swarm spore root inoculating method of the invention can do inoculation strictly and quantificationally, and avoids overlarge inoculum concentration to result in that the original disease resistant variety is in susceptibility, thereby ensuring to be more effective and accurate in the application of the filtration of the anti-source; at the same time, the method of the invention has the advantages of small occupied space, less labors, short experiment period and simple latter material treatment in the actual operation, therefore, the method of the invention is suitable for popularization and application.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY
Real-time observation method for phytophthora nicotianae infection process
The invention discloses a real-time observation method for phytophthora nicotianae infection process. The real-time observation method for the phytophthora nicotianae infection process includes that preparing for tobacco seed plantlet, preparing phytophthora nicotianae zoospore suspension, irrigating the root to inoculate, dyeing and observing. According to the real-time observation method for the phytophthora nicotianae infection process, phytophthora nicotianae inoculation can be performed in a manual climatic box or a culture room by means of culture dish sponge and filter paper for preserving moisture, and the dyed and discolored seed plantlet radicle can be directly observed under a microscope. The real-time observation method for the phytophthora nicotianae infection process is convenient and easy to operate and suitable for observing the phytophthora nicotianae infection process of tobacco and detecting the resistance of different varieties.
Owner:YUNNAN ACAD OF TOBACCO AGRI SCI
Method for separating monosporangiums of potato late blight pathogens
InactiveCN105483032AShorten the timeImprove operational efficiencyFungiSpore processesMicroorganismGenetic Materials
The invention belongs to the field of microorganisms, and particularly relates to a method for separating monosporangiums of potato late blight pathogens. On the basis of an existing suspension liquid separating method, the monosporangiums are separated through the characteristic that each monosporangium contains a plurality of zoospores, and genetic materials of the zoospores are the same; the monosporangiums are stimulated at the low temperature to release the zoospores, and the new method for separating the monosporangiums is formed. The success rate of the improved method is nearly 6 times that of the original method, and operation time is halved; meanwhile, operative difficulty is greatly reduced, and the method is an effective method for rapidly separating a large mass of monosporangiums in a pathogeny-group researching test.
Owner:FUJIAN AGRI & FORESTRY UNIV
Method for cultivating undaria pinnatifida and using undaria pinnatifida to treat red tides
InactiveCN107950380AReduce eutrophicationRed tide avoidSeawater treatmentCultivating equipmentsEutrophicationOrganism
The invention provides a method for cultivating undaria pinnatifida and using the undaria pinnatifida to treat red tide. The method is characterized in that according to a sign before the occurrence of the red tides, that is, the temperature is increased by 2 DEG C within 7 days, a method different from a biological restraint method is adopted; before the outbreak of the red tides, the cultivatedundaria pinnatifida is added into the sea to absorb nutritional components in the sea water to alleviate the eutrophication of the sea water, and thus the occurrence of the red tides is avoided. The method includes: performing induced cultivation on female gametophyte to obtain young sporophyte, and carrying out aerated feeding cultivation to obtain mature sporophyte; releasing the sporophyll of the sporophyte into the sea to obtain zoospores, and placing seedling curtains attached with the zoospores in fresh sea water; when the germinated zoospores develop into female gametophyte, co-cultivating male gametophyte and the female gametophyte attached to the seedling curtains to complete the development and fertilization process; transferring the young sporophyte to a relevant sea area for cultivation after the young sporophyte reaches 50-100 micrometers to obtain the undaria pinnatifida and treat the red tides.
Owner:BIOTECH RES CENT SHANDONG ACADEMY OF AGRI SCI
Phytophthora culture medium and preparation method thereof
The present invention provides a phytophthora culture medium and a preparation method thereof, wherein 20-40 g of rice is added to 400-600 ml of water, water boiling bath heating is performed for 10-20 min to obtain rice water, the rice water is mixed with 400-600 ml of a soil leaching solution and 4-6 g of sunflower seed powder, and sterilization is performed to obtain the finished product. According to the present invention, the phytophthora culture medium provides a good culture effect for phytophthora, and the number of the zoosporangium of the phytophthora is more; and the phytophthora culture medium preparation method has characteristics of simpleness, wide raw material source, and simple and easily-available raw material, and is suitable for industrial production preparation.
Owner:YUNNAN AGRICULTURAL UNIVERSITY
Method for evaluating pepper phytophthora blight resistance of crops
InactiveCN106636302APrecise screeningEfficient screeningMicrobiological testing/measurementPhytophthora sp.Mildew
The invention discloses a method for evaluating the pepper phytophthora blight resistance of a crop. The method comprises the specific steps of preparation of seedlings, preparation of phytophthora capsici zoospore suspension liquid, root-irrigation and inoculation, statistics of morbidity and evaluation of a disease-resistant result. Quantitative phytophthora capsici zoospores are inoculated into the seedling root parts of the crop, it is avoided that the same variety anti-infection shows difference due to excessive inoculation amount, and during resistance identification, the method is more accurate and effective; in the actual operation, inoculation can be conducted in a large batch mode, and the method has the advantages of being small in occupied space, saving labor cost and shortening test period. Accordingly, the method is suitable for application and popularization.
Owner:INST OF PLANT PROTECTION FAAS
Downy mildew bacterium culture and observation method
The invention relates to the technical field of biology, in particular to a downy mildew bacterium culture and observation method. The method comprises the following steps: collecting downy mildew bacteria; preparing sporangium suspension liquid; and culturing the sporangium suspensions. Through repeated tests, a technology capable of inducing sporangium of cucumber downy mildew to germinate andrelease zoospore is obtained, the zoospore forms static spores through movement and morphological changes, the static spores germinate to form appressorium, and the appressorium germinates to generateinfection filaments. The technology can provide an effective technique for researchers to deeply research downy mildew biological basis, disease resistance identification, bactericide screening and the like. By means of the method, researchers can achieve quantitative statistics and monitoring of the number of zoospore of downy mildew, morphological changes and the number of formed infectors, andresearch of the action mechanism of the bactericide can also be achieved. Several development stages which must be experienced before downy mildew bacteria invade a host are clearly observed. The systematic observation method can provide first-hand data for prevention and treatment of the pathogenic bacteria on symptomatic drugs.
Owner:NINGXIA UNIVERSITY
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