15 results about "Zoospore" patented technology

The invention provides a streptomyces flavogriseus C-21 strain, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the registration number of the streptomyces flavogriseus C-21 strain is CGMCC No. 36050. The preparation method of the sterile fermentation filtrate of the streptomyces fulvus C-21 comprises the following steps: inoculating a strain C-21 to an oat culture medium plate in a streak manner, culturing for 7 days at 28 DEG C, scraping spores on the surface of the culture medium, adding sterile water to prepare a spore suspension with the concentration of 108 / mL, inoculating the spore suspension into a millet immersion culture solution according to the inoculum size of 2%, and culturing for 7 days at the temperature of 28 DEG C to obtain the sterile fermentation filtrate of the streptomyces fulvus C-21. Performing shaking culture for 7 days in a constant-temperature shaking table at the temperature of 28 DEG C and the speed of 180 r / min, centrifuging for 30 minutes at the speed of 12000 r / min, and filtering supernate by using a 0.22 mu m microporous filter membrane to obtain sterile fermentation filtrate. The streptomyces fulvus C-21 can generate extracellular enzymes such as cellulase, protease and amylase, sterile fermentation filtrate of the streptomyces fulvus C-21 can destroy mycelial morphology and cell membrane integrity of the tomato late blight bacteria, mycelial growth, sporangium germination and zoospore release of the tomato late blight bacteria are remarkably inhibited, and the streptomyces fulvus C-21 has a good control effect on the tomato late blight.

The application provides a kelp breeding performance evaluation method based on sporangium spore dissemination parameters and application, and belongs to the technical field of seaweed cultivation. The method comprises the following steps: S1, taking mature kelp samples, washing, and air-drying treatment to obtain pretreated kelp; S2, taking kelp tissue blocks from the pretreated kelp obtained in step S1, loading the kelp tissue blocks into a mesh bag, immersing the kelp tissue blocks in seawater, promoting the kelp tissue blocks to disseminate, and collecting zoospore liquid; S3, measuring sporangium spore dissemination parameters in step S2; S4, calculating dissemination capacity according to the sporangium spore dissemination parameters obtained in step S3, and evaluating the breeding performance of the kelp according to the dissemination capacity. The above method can quantify the sporangium spore dissemination characteristics of kelp, systematically evaluate the breeding potential of kelp, and improve the breeding efficiency and yield of kelp.

This invention discloses an oligosaccharide syltransferase STT3 subunit from *Phytophthora sojae*, its encoding gene, and its applications. The protein sequence of the oligosaccharide syltransferase STT3 subunit provided by this invention is shown in Sequence 2; its encoding gene is shown in Sequence 1. Experiments have demonstrated that the protein provided by this invention plays an important role in the growth and development of *Phytophthora sojae*, specifically manifested in the slowed mycelial growth, reduced number of sporangia and zoospores, and decreased pathogenicity of *Phytophthora sojae* after its deletion. These conclusions provide a technical basis for exploring the molecular mechanisms of *Phytophthora sojae* development and pathogenicity, and provide molecular targets for the future development of novel fungicides.

The invention discloses a pseudomonas strain JQ36 and a preparation method and application of a peptide compound produced by the pseudomonas strain JQ36. The pseudomonas JQ36 is separated from rhizosphere soil of wheat in Gansu province, and peptide substances with novel structures can be generated through liquid fermentation. The substances can be separated and purified by combining a solid-phase extraction technology with a high performance liquid chromatography technology to obtain monomeric compounds. Under in-vitro experiment conditions, the peptide substance can inhibit the zoospore germination of phytophthora capsici and split the zoospore of phytophthora capsici; in a pepper living body, the compound with the concentration of 50 mu M can well prevent and control phytophthora capsici diseases, and compared with a control group, the compound with the concentration can achieve the phytophthora capsici prevention and control effect of 83% or above. Therefore, the compound with the novel structure has a good phytophthora capsici prevention and control effect, can be used for preparing biological medicines for preventing and controlling phytophthora capsici, and has an important potential application value in the fields of agricultural production and the like.

The invention discloses a glucosidase protein (GLS1) from phytophthora plant pathogenic oomycetes as well as a coding gene and application thereof. The PsGLS1 protein provided by the invention is a protein of which the sequence is as shown in SEQ ID No. 1 in Phytophthora sojae, and the PsGLS1 protein is a protein of which the sequence is as shown in SEQ ID No. 2 in Phytophthora sojae; the coding gene of the gene is shown as SEQ ID No.2. Experiments prove that the protein provided by the invention plays an important role in the growth and development process of phytophthora sojae, and after the PsGLS1 gene is knocked out, the mycelial growth rate, the sporangium number, the zoospore yield and the resting spore germination rate of the phytophthora sojae are obviously reduced, and the pathogenicity of pathogenic bacteria is obviously influenced. Glucosidase inhibitor mispermine has an inhibition effect on the growth of phytophthora sojae hyphae. In conclusion, the PsGLS1 gene has very high application value in control of epidemic disease occurrence and epidemic caused by phytophthora, and a molecular target is provided for research and development of novel bactericides in the future.

This invention discloses a glucosidase protein (GLS1) from *Phytophthora*, a plant pathogenic oomycete, its encoding gene, and its applications. The PsGLS1 protein provided by this invention is effective against *Phytophthora soybean* (…). Phytophthora sojae The protein in this invention is shown in SEQ ID No. 1; its encoding gene is shown in SEQ ID No. 2. Experiments have demonstrated that the protein provided by this invention plays an important role in the growth and development of *Phytophthora soybeani*. PsGLS1 Gene knockout significantly reduced the mycelial growth rate, sporangium number, zoospore yield, and resting spore germination rate of *Phytophthora soybeani*, and also significantly affected the pathogenicity of the fungus. The glucosidase inhibitor sericite also inhibited the mycelial growth of *Phytophthora soybeani*. In summary, this invention… PsGLS1 Genes have high application value in controlling the occurrence and spread of diseases caused by Phytophthora, and provide molecular targets for the development of new fungicides in the future.

The application discloses an oligosaccharyltransferase STT3A (staurosporine and temperature-sensitive A) subunit from Phytophthora capsici and a coding gene and application thereof. The oligosaccharyltransferase STT3A subunit provided by the application is shown as sequence 2; and the coding gene is shown as sequence 1. Experiments prove that the protein provided by the application plays an important role in the growth and development of Phytophthora capsici, and the specific performance is that after the protein is deleted, the mycelium growth rate of Phytophthora capsici is slowed down, the zoospore quantity is reduced, and the pathogenicity is reduced, etc. The above conclusion provides a technical basis for exploring the development and pathogenic molecular mechanism of Phytophthora capsici, and provides a potential molecular target for the research and development of a new type of bactericide in the future.

This invention discloses a multi-layer vertical seedling device and method for kelp. The seedling device includes a seedling frame and several layers of seedling trays. The height of the seedling trays is adjustable. A dynamic shading curtain is installed at the top of the seedling frame. A supplemental lighting unit, a flow control unit, and an environmental monitoring unit are installed on each seedling tray. Normal seedling cultivation is performed on the first layer of seedling trays. In the early stage of seedling attachment on the second layer of seedling trays, white light is emitted by the supplemental lighting unit of the second layer to promote the attachment and germination of zoospores to form gametophytes. Subsequently, the supplemental lighting unit of the second layer of seedling trays emits red and blue light, with the proportion of red light being greater than that of blue light. During the extended cultivation stage on the second layer of seedling trays, moderate light intensity red light is used as the main source and blue light as a supplement to slow growth while ensuring the accumulation of photosynthetic products, increasing the chlorophyll content of seedlings, enhancing adhesion, reducing diseases and seedling rot, and improving the stability and yield of seedlings.

The application provides application of chitin in early infection of plasmodiophora brassicae in prevention and control of brassica root tumour disease, and is based on the technology and application of blocking chitin synthesis of zoosporangium in early infection of plasmodiophora brassicae to prevent and control brassica root tumour disease. It is proved by experiments that the zoosporangium in early infection of plasmodiophora brassicae contains chitin, and the chitin is synthesized by plasmodiophora brassicae chitin synthase genes PbCHS3 and PbCHS7, thereby providing a new target for prevention and control of brassica root tumour disease. Through expression of PbCHS3 and PbCHS7 silencing vectors, exogenous small RNA treatment or application of polyoxin, which can block chitin synthesis, in arabidopsis and rape, brassica root tumour disease can be prevented and controlled efficiently, and key technology and method for prevention and control of brassica root tumour disease are provided.

The invention relates to germplasm preservation, propagation and seedling cultivation of undaria pinnatifida, in particular to a method for large-scale updating and rapid propagation of undaria pinnatifida gametophyte germplasm. Specifically, obtained mature homozygous double (DH) sporophytes are dried in shade and stimulated to diffuse a large number of zoospores, and the spores germinate to form gametophytes consistent with parent genotypes, so that large-scale updating and rapid propagation of gametophyte germplasm are realized. The method disclosed by the invention effectively solves the technical bottleneck that a large number of gametophyte clones are difficult to obtain in a short time due to slow cell division of gametophytes from single cells, and has important significance on creation of new undaria pinnatifida hybrid varieties and large-scale seed production.

This invention discloses an arginine methyltransferase protein from *Phytophthora* plant pathogenic oomycetes, its encoding gene, and its applications. The arginine methyltransferase protein provided by this invention is shown in sequences 6-8; its encoding gene is shown in sequences 1-3. Experiments have demonstrated that the protein of this invention plays an important role in the growth, development, and pathogenicity of *Phytophthora capsici*, a typical plant pathogenic oomycete. The absence or reduced expression level of this protein leads to slower mycelial growth rate, reduced sporangium production, decreased zoospore numbers, and reduced pathogenicity of *Phytophthora capsici*. Corresponding arginine methyltransferase inhibitors exhibit varying degrees of activity against typical plant pathogenic oomycetes such as *Phytophthora capsici*, *Phytophthora soybeani*, and *Phytophthora pathogenica*. The gene provided by this invention has significant application potential in the prevention and control of crop blight caused by *Phytophthora* plant pathogenic oomycetes. Novel fungicides developed based on this protein as a target have important practical significance for controlling the occurrence and spread of crop blight caused by *Phytophthora*.

The application discloses an oligosaccharyltransferase STT3B subunit from a phytophthora capsici and a coding gene and application thereof. The oligosaccharyltransferase STT3B subunit provided by the application is shown as sequence 2; and the coding gene is shown as sequence 1. Experiments prove that the protein provided by the application plays an important role in the growth and development of the phytophthora capsici, and the specific performance is that after the protein is deleted, the mycelium growth rate of the phytophthora capsici is slowed down, the zoospore quantity is reduced, the pathogenicity is reduced and the like. The above conclusion provides a technical basis for exploring the development and pathogenic molecular mechanism of the phytophthora capsici, and provides a potential molecular target for the research and development of a new type of bactericide in the future.

An epiphytic apparatus includes a bottomed water tank, a deposition-bed tool on which zoospores released from a mother alga can be deposited, and a vertical light source capable of emitting light toward the zoospores from below. The epiphytic apparatus further includes a plurality of brightness detectors disposed above the bottomed water tank and capable of detecting a brightness distribution of the vertical light source, a distribution calculation unit that calculates a 3D distribution of zoospores based on brightness detection results of the plurality of brightness detectors, and a swimming control unit that controls, based on the 3D distribution of the zoospores, the swimming of the zoospores so that the zoospores swim toward the deposition-bed tool.

This invention discloses genes PlSWL1 And its application in the prevention and control of downy mildew in litchi. This invention constructs... PlSWL1 The gene was knocked out via vector and transformed into a protoplast using PEG-mediated homologous recombination. PlSWL1 Knockout mutants and replacement transformants were obtained from *Phytophthora licoricei* through knockout and replacement. Compared with the wild type, the mutants showed a significantly reduced germination rate of dormant spores and a markedly weakened pathogenicity of zoospores and hyphae. This invention confirms... PlSWL1 It is essential for the growth, development, infection, and pathogenicity of Phytophthora lichei, and helps to elucidate the pathogenic molecular mechanism of Phytophthora lichei, providing a target gene for the development of effective fungicides.