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Method for cultivating undaria pinnatifida and using undaria pinnatifida to treat red tides

A technology of wakame and red tide, applied in chemical instruments and methods, botanical equipment and methods, applications, etc., can solve the problems of difficult wide application, large-scale application, long preparation cycle, selectivity limitation, etc., to avoid the occurrence of red tide, The method is simple and has no effect of secondary pollution

Inactive Publication Date: 2018-04-24
BIOTECH RES CENT SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to selectivity limitations, long preparation cycle, high cost and other reasons, this method is difficult to be widely and large-scale application
And a virus often only has specific killing activity on one species of algae

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for cultivating undaria pinnatifida and controlling red tides, comprising the following steps:

[0022] 1) Induce the female gametophyte until the young sporophyte reaches 0.8-1.0 mm, then fill it with air, maintain the temperature at 18°C, light intensity 2000Lx, light time 12 h / day, and exchange the culture medium once every 2 days. Cultivate until the young sporophytes reach 0.8-1.2cm in length, and form parthenogenetically mature sporophytes;

[0023] The induction culture medium is: add 20mL preparation solution to every 1L of sterilized seawater; the preparation solution is to add NaNO to every 1L of distilled water 3 3.5g, KH 2 PO 4 0.5g, Fe(NH 4 ) 2 (SO 4 ) 2 ·6H 2 O 0.702g, Na 2 EDTA 1g,H 3 BO 3 1.14g, MnSO 4 ·H 2 O 164m, dissolved and sterilized.

[0024] The induction culture conditions are: temperature 18°C, light intensity 2000Lx, and light time 12 h / day for 12 days, and the culture medium is fully exchanged once every 2 days.

[0...

Embodiment 2

[0031] A method for cultivating undaria pinnatifida and controlling red tides, comprising the following steps:

[0032] 1) Induce the female gametophyte until the young sporophyte reaches 0.8-1.0 mm, then fill it with air, maintain the temperature at 18°C, light intensity 2000Lx, light time 12 h / day, and exchange the culture medium once every 2 days. Cultivate until the young sporophytes reach 0.8-1.2cm in length, and form parthenogenetically mature sporophytes;

[0033] The induction culture medium is: add 20mL preparation solution to every 1L of sterilized seawater; the preparation solution is to add NaNO to every 1L of distilled water 3 3.5g, KH 2 PO 4 0.5g, Fe(NH 4 ) 2 (SO 4 ) 2 ·6H 2 O 0.702g, Na 2 EDTA 1g,H 3 BO 3 1.14g, MnSO 4 ·H 2 O 164m, dissolved and sterilized.

[0034] The induction culture conditions are: temperature 18°C, light intensity 2000Lx, and light time 12 h / day for 12 days, and the culture medium is fully exchanged once every 2 days.

[0...

Embodiment 3

[0041] A method for cultivating undaria pinnatifida and controlling red tides, comprising the following steps:

[0042] 1) Induce the female gametophyte until the young sporophyte reaches 0.8-1.0 mm, then fill it with air, maintain the temperature at 20°C, light intensity 2000Lx, light time 12 h / day, and exchange the culture medium once every 2 days. Cultivate until the young sporophytes reach 0.8-1.2cm in length, and form parthenogenetically mature sporophytes;

[0043] The induction culture medium is: add 20mL preparation solution to every 1L of sterilized seawater; the preparation solution is to add NaNO to every 1L of distilled water 3 3.5g, KH 2 PO 4 0.5g, Fe(NH 4 ) 2 (SO 4 ) 2 ·6H 2 O 0.702g, Na 2 EDTA 1g,H 3 BO 3 1.14g, MnSO 4 ·H 2 O 164m, dissolved and sterilized.

[0044] The induction culture conditions are: temperature 18°C, light intensity 2000Lx, and light time 12 h / day for 12 days, and the culture medium is fully exchanged once every 2 days.

[0...

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PUM

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Abstract

The invention provides a method for cultivating undaria pinnatifida and using the undaria pinnatifida to treat red tide. The method is characterized in that according to a sign before the occurrence of the red tides, that is, the temperature is increased by 2 DEG C within 7 days, a method different from a biological restraint method is adopted; before the outbreak of the red tides, the cultivatedundaria pinnatifida is added into the sea to absorb nutritional components in the sea water to alleviate the eutrophication of the sea water, and thus the occurrence of the red tides is avoided. The method includes: performing induced cultivation on female gametophyte to obtain young sporophyte, and carrying out aerated feeding cultivation to obtain mature sporophyte; releasing the sporophyll of the sporophyte into the sea to obtain zoospores, and placing seedling curtains attached with the zoospores in fresh sea water; when the germinated zoospores develop into female gametophyte, co-cultivating male gametophyte and the female gametophyte attached to the seedling curtains to complete the development and fertilization process; transferring the young sporophyte to a relevant sea area for cultivation after the young sporophyte reaches 50-100 micrometers to obtain the undaria pinnatifida and treat the red tides.

Description

technical field [0001] The invention relates to a method for controlling red tides, in particular to a method for cultivating wakame and controlling red tides. Background technique [0002] Wakame (Undaria pinnatifida) belongs to Phaeophyta, Brownia, Laminaria, Pteraceae, and Undaria genus. It belongs to euthermic algae. It is delicious and nutritious. , sugar and vitamins, because the cellulose content of wakame is only about 3% of the dry weight, it is easy to be digested by the human body, and it is a healthy food popular among consumers. Wakame contains a variety of nutrients. According to preliminary analysis, every 100 grams of dry product contains 11.6g of crude protein, 0.32g of refined fat, 37.81g of sugar, and 18.93g of ash. It also contains a variety of vitamins. The crude protein content is higher than that of kelp , and its taste is also more than kelp. Undaria pinnatifida is not only an edible economical brown algae, but also can be used as a raw material for...

Claims

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Application Information

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IPC IPC(8): A01G33/00C02F3/32C02F103/08
CPCA01G33/00C02F3/322C02F2103/08
Inventor 黄超尤升波于金慧毕玉平
Owner BIOTECH RES CENT SHANDONG ACADEMY OF AGRI SCI
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