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Phytophthora culture medium and preparation method thereof

A culture medium, Phytophthora technology, applied to bacteria and other directions, can solve problems such as unsatisfactory Phytophthora culturing effect, and achieve the effects of simple preparation method, good culturing effect and wide sources

Inactive Publication Date: 2014-03-26
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide a Phytophthora culture medium, aiming to solve the problem that the existing oatmeal culture medium has an unsatisfactory effect on the cultivation of Phytophthora

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] A preparation method for Phytophthora culture medium, comprising the following steps:

[0014] (1) Add 20g of rice to 500ml of water, place the container containing water and rice in boiling water and heat for 15 minutes, filter the mixture in the container after heating to obtain rice soup;

[0015] (2) Add 600ml of soil extract and 6g of sunflower seed powder to the rice soup prepared in step (1), and sterilize it to obtain Phytophthora medium 1.

Embodiment 2

[0017] (1) Add 40g of rice to 400ml of water, place the container containing water and rice in boiling water and heat for 20 minutes, filter the mixture in the container after heating to obtain rice soup;

[0018] (2) Add 400ml of soil extract and 4g of sunflower seed powder to the rice soup prepared in step (1), and sterilize it to obtain Phytophthora medium 2.

Embodiment 3

[0020] (1) Add 30g of rice to 500ml of water, heat the container containing water and rice in boiling water for 10 minutes, and filter the mixture in the container after heating to obtain rice soup;

[0021] (2) Add 500ml of soil extract and 5g of sunflower seed powder to the rice soup prepared in step (1), and sterilize it to obtain Phytophthora medium 3.

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PUM

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Abstract

The present invention provides a phytophthora culture medium and a preparation method thereof, wherein 20-40 g of rice is added to 400-600 ml of water, water boiling bath heating is performed for 10-20 min to obtain rice water, the rice water is mixed with 400-600 ml of a soil leaching solution and 4-6 g of sunflower seed powder, and sterilization is performed to obtain the finished product. According to the present invention, the phytophthora culture medium provides a good culture effect for phytophthora, and the number of the zoosporangium of the phytophthora is more; and the phytophthora culture medium preparation method has characteristics of simpleness, wide raw material source, and simple and easily-available raw material, and is suitable for industrial production preparation.

Description

technical field [0001] The invention belongs to the field of bacterial culture medium preparation, and in particular relates to a Phytophthora culture medium and a preparation method thereof. Background technique [0002] Now the cultivation of Phytophthora generally adopts oatmeal to add water, boils to get the filtrate, and then packs and sterilizes to obtain. However, Phytophthora cultured on this oatmeal medium had fewer zoosporangia. Contents of the invention [0003] The object of the present invention is to provide a Phytophthora culture medium, aiming to solve the problem that the existing oatmeal culture medium has unsatisfactory cultivation effect on Phytophthora. [0004] The present invention is achieved in this way. A Phytophthora culture medium includes soil extract, sunflower seed powder, and rice, wherein the volume-to-mass ratio of the soil extract, sunflower seed powder, and rice is (400-600) ml: (4 ~6)g: (20~40)g. [0005] Preferably, the volume-to-ma...

Claims

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Application Information

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IPC IPC(8): C12N1/20
Inventor 彭晟陈微陈兴杨莹赵银河
Owner YUNNAN AGRICULTURAL UNIVERSITY
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