Screening method of biocontrol bacteria and dosage for tobacco black shank

A technology of tobacco black shank and tobacco black shank, which is applied in the field of biological control of crop diseases, can solve the problems of unfavorable development of plant disease biological control, a large amount of test materials and labor, and restriction of biological control bacterial strains, etc., to achieve screening results Accurate and reliable, saving manpower and less workload

Inactive Publication Date: 2011-12-14
GUIZHOU TOBACCO SCI INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The selection of existing bio-control bacterial strains is often achieved through the following steps. The culture dish face-off culture method is used to screen the bacteria with the antibacterial zone effect, and then the live potted plant is used to verify the disease prevention effect. This method often has a large workload and slow speed. , A large amount of test materials and labor are required, and the cycle is generally more than 90 days, which seriously restricts the selection of biocontrol bacterial strains
At the same time, many biocontrol bacteria have an inhibitory effect on pathogenic bacteria on the isolated plate, but have no control effect on the living body; while some antagonistic bacteria have a good control effect on the living body, but have no activity on the isolated plate. Therefore, there are many loopholes in the screening of antagonistic bacteria by using the Petri dish confrontation culture method, and there is a certain probability that good biocontrol bacteria will be lost, which is not conducive to the development of biological control of plant diseases.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: take tobacco black shank as an example to describe the screening of this biocontrol bacterium in detail, and its steps are as follows:

[0021] a. Plant aseptic tobacco seedlings on filter paper in more than one group of petri dishes. The number of petri dishes in a group is generally 3, and 12 tobacco seedlings (variety: Honghua Dajinyuan) are planted in each dish. The petri dishes used The diameter is 90mm, and the nutrient solution is added to the petri dish. The time is generally 5-7 days. After the two cotyledons of the tobacco seedlings are fully unfolded, the standard of the in vivo test can be reached;

[0022] b. After obtaining the tobacco seedlings used for the live test, add culture fluid of unknown bacterial strains with biocontrol potential to the petri dish for protective activity screening; unknown bacterial strains with biocontrol potential are obtained by the following steps:

[0023] (1) Select the soil and tobacco leaves of tobacco plan...

Embodiment 2

[0030] Embodiment 2: take tobacco black shank as an example to describe in detail the concentration screening of chemical bactericide metalaxyl to prevent and treat this disease, and its steps are as follows:

[0031] a, plant aseptic seedlings on the filter paper in more than one group of petri dishes, the number of one group of petri dishes is 7, plant 12 strains of tobacco seedlings in every petri dish, and the diameter of the petri dish used is 90mm, in the petri dish Add nutrient solution, the time is generally 5-7 days, when the two cotyledons of the tobacco seedlings are fully unfolded, they can be used for the screening of drug concentration;

[0032] b. Prepare metalaxyl liquid medicines with serial concentrations of 25, 12.5, 6.25, 3.125, 1.5625, 0.78, and 0 mg / L;

[0033] In petri dish, apply the zoospore suspension of band black shank disease bacterium; The quantity of the zoospore suspension of the tobacco black shank disease bacterium that applies in each petri d...

Embodiment 3

[0037] Embodiment 3: take tobacco black shank as an example to describe the screening of this biocontrol bacterium in detail, and its steps are as follows:

[0038] a. Plant aseptic tobacco seedlings on filter paper in more than one group of petri dishes. The number of petri dishes in a group is generally 3, and 12 tobacco seedlings (variety: Honghua Dajinyuan) are planted in each dish. The petri dishes used The diameter is 90mm, and the nutrient solution is added to the petri dish. The time is generally 5-7 days. After the two cotyledons of the tobacco seedlings are fully unfolded, the standard of the in vivo test can be reached;

[0039]B, after obtaining the tobacco seedling that live test is used, add the zoospore suspension of tobacco black shank bacterium in petri dish and carry out therapeutic activity screening;

[0040] c. After the zoospore suspension of tobacco seedlings and tobacco black shank bacterium was cultivated together for 24 hours, the unknown bacterial st...

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Abstract

The invention discloses a method for screening biocontrol bacteria and dosage thereof against tobacco black shank. The method comprises the following steps: a. cultivating sterile tobacco seedlings on filter paper in a petri dish; b. on the basis of step a , add bacterial culture solution with biocontrol potential in the petri dish; c, after the tobacco seedlings and the bacterial suspension with biocontrol potential are cultivated together for 24 hours, add the zoospore suspension of tobacco black shank bacteria to the petri dish d, on the basis of step c, give the tobacco seedlings normal growth conditions, detect the control effect of the bacteria with biocontrol potential added by observing the morbidity of the tobacco seedlings after 7 days, and screen out the biocontrol that can prevent and treat tobacco black shank bacteria. Compared with the traditional method, the present invention has many advantages. It is simple, fast, less workload, high efficiency, accurate and reliable screening result, and can screen bacteria with bio-control function from a large number of unknown bacteria in a short period of time.

Description

technical field [0001] The invention relates to a method for screening biocontrol bacteria and dosage for tobacco black shank, and belongs to the technical field of crop disease biological control. Background technique [0002] With the development of society, people pay more and more attention to the harm of smoking to the body, and the control on the application of chemical fertilizers and pesticides in the process of tobacco leaf production is becoming more and more intense. A large number of studies have shown that many diseases of tobacco can be eliminated through biological The use of bio-control bacteria to prevent and control plant diseases is the key direction of future development; one of the difficulties in the control of plant diseases by bio-control bacteria is the screening of highly efficient bio-control bacteria. The selection of existing bio-control bacterial strains is often achieved through the following steps. The culture dish face-off culture method is u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12R1/645
Inventor 汪汉成石俊雄李文红夏海乾王晶君
Owner GUIZHOU TOBACCO SCI INST
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