Screening method of biocontrol bacteria and dosage for tobacco black shank

Abstract

The invention discloses a method for screening biocontrol bacteria and dosage thereof against tobacco black shank. The method comprises the following steps: a. cultivating sterile tobacco seedlings on filter paper in a petri dish; b. on the basis of step a , add bacterial culture solution with biocontrol potential in the petri dish; c, after the tobacco seedlings and the bacterial suspension with biocontrol potential are cultivated together for 24 hours, add the zoospore suspension of tobacco black shank bacteria to the petri dish d, on the basis of step c, give the tobacco seedlings normal growth conditions, detect the control effect of the bacteria with biocontrol potential added by observing the morbidity of the tobacco seedlings after 7 days, and screen out the biocontrol that can prevent and treat tobacco black shank bacteria. Compared with the traditional method, the present invention has many advantages. It is simple, fast, less workload, high efficiency, accurate and reliable screening result, and can screen bacteria with bio-control function from a large number of unknown bacteria in a short period of time.

Description

technical field [0001] The invention relates to a method for screening biocontrol bacteria and dosage for tobacco black shank, and belongs to the technical field of crop disease biological control. Background technique [0002] With the development of society, people pay more and more attention to the harm of smoking to the body, and the control on the application of chemical fertilizers and pesticides in the process of tobacco leaf production is becoming more and more intense. A large number of studies have shown that many diseases of tobacco can be eliminated through biological The use of bio-control bacteria to prevent and control plant diseases is the key direction of future development; one of the difficulties in the control of plant diseases by bio-control bacteria is the screening of highly efficient bio-control bacteria. The selection of existing bio-control bacterial strains is often achieved through the following steps. The culture dish face-off culture method is u...

AI Technical Summary

Problems solved by technology

The selection of existing bio-control bacterial strains is often achieved through the following steps. The culture dish face-off culture method is used to screen the bacteria with the antibacterial zone effect, and then the live potted plant is used to verify the disease prevention effect. This method often has a large workload and slow speed. , A large amount of test materials and labor are required, and the cycle is generally more than 90 days, which seriously restricts the selection of biocontrol bacterial strains

At the same time, many biocontrol bacteria have an inhibitory effect on pathogenic bacteria on the isolated plate, but have no control effect on the living body; while some antagonistic bacteria have a good control effect on the living body, but have no activity on the isolated plate. Therefore, there are many loopholes in the screening of antagonistic bacteria by using the Petri dish confrontation culture method, and there is a certain probability that good biocontrol bacteria will be lost, which is not conducive to the development of biological control of plant diseases.