34results about How to "Exempt investment" patented technology

The invention relates to a power system energy efficiency public service cloud platform based on cloud computing. A three-layer framework is adopted in the energy efficiency public service cloud platform and comprises an energy efficiency service Iaas layer, an energy efficiency service Paas layer and an energy efficiency service application Saas layer. The energy efficiency service Iaas layer is an infrastructure level service layer and is used for providing virtual resources, mapped through the virtual technology, for a network, a server and database physical facilities in the cloud platform. The energy efficiency service Paas layer is a platform level service layer and used for providing the execution environment of cloud middleware resources, a distributed parallel platform and application service software for power consumers. The energy efficiency service application Saas layer is a software level service layer and used for providing software services of energy efficiency services based on Web access for users. According to the energy efficiency public service cloud platform, acquisition and monitoring of energy efficiency information and diagnosis and analysis of user energy efficiency parameters are achieved through the energy efficiency service application Saas layer. By means of the framework mode, management objects and function requirements can be quickly expanded and upgraded, and user energy management efficiency is improved.

The invention provides loop-mediated isothermal amplification (LAMP) primers for detecting Phytophthora capsici and a kit comprising the primers. The primers comprise FIP, BIP, F3 and B3 (shown as Seq ID No.1-4). An LAMP primer technology is used for quickly detecting the Phytophthora capsici, and the Phytophthora capsici can be accurately detected in a complicated pathogenic bacteria environmentin diseased plant tissues and soil. The method has higher specificity and sensitivity than the conventional PCR method, propagules of the Phytophthora capsici in various forms, such as mycelia, oospores and zoospores can be detected, and the method has significance in aspects of early warning of Phytophthora capsici epidemics and monitoring pathogeny in epidemic areas; meanwhile, expensive instruments can be avoided, and the method is easily promoted and used in basic level.

The invention provides a RPA primer, probe and detection method for detecting salmonella. A primer sequence of the RPA primer is as follows: for an iroB gene: an upstream primer SEQ ID No.1:5'-GGAATGTCATACTTAGCGGGTTTGACACGAGC-3', and a downstream primer SEQ ID No.2:5'-GGTGGTATTTGACGCTGGCGGTGCAAACCT-3'. According to the invention, an RPA technology is combined with fluorescence detection, the salmonella can be quickly and accurately detected. By adopting the method, the salmonella can be specifically detected from common pathogenic bacteria, thereby providing a technical support for rapid detection of the salmonella, and at the same time, the investment of expensive instruments can be eliminated, and the method is convenient for popularization and use at the grassroots level.

The invention discloses a RPA primer for detecting chicken infectious laryngotracheitis virus. The nucleotide sequence of the RPA primer is shown as SEQ ID NO.1 and SEQ ID NO.2; the RPA primer is strong in specificity, high in sensitivity, and accurate in detection result. The invention further provides a RPA method for detecting the chicken infectious laryngotracheitis virus. The detecting method is easy to operate, low in equipment requirement, strong in specificity, high in sensitivity, and accurate and reliable in detection result; the detecting method provides a low-cost, fast and specific field diagnosis method for effectively detecting and diagnosing the chicken infectious laryngotracheitis virus.

The invention provides a rapid detection method of food-borne burkholderia gladioli, which comprises the following steps: a polymerase chain reaction is carried out in a polymerase reaction system, wherein the reaction system contains a specific primer pair for amplifying burkholderia gladioli and a burkholderia gladioli specific probe; the specific primer pair for amplifying the burkholderia gladioli comprises primers as shown in SEQ ID NO.1 and SEQ ID NO.2, the burkholderia gladioli specific probe is a Taqman probe. The rapid detection method for the food-borne burkholderia gladioli established by the invention has the characteristics of strong specificity, high sensitivity, rapid detection and the like, can meet the requirements of daily rapid food detection, can avoid the investment of expensive instruments, is convenient to popularize and use in grassroots, and has a relatively good application prospect.

The invention provides a RPA primer and a probe for detecting Listeria monocytogenes and a detection method. The RPA primer and the probe are designed based on a Listeria monocytogenes actA gene, andprimer sequences are shown in SEQ ID No.1, 2 and 3. The probe length is 45-52bp, a position where is 30bp apart from 5' terminal in the middle of the probe is modified by dSpacer, thymidine at doublesides of the dSpacer molecule are respectively replaced by a fluorescence group FAM and a quenching group BHQ1, a 3' terminal of the probe is modified by a blocking group C3Spacer, and the sequence isshown in SEQ ID No.4. Compared with the prior art, detection by the detection method can be completed within 15 minutes, in order to rapidly detect whether Listeria monocytogenes exists in a sample;the established RPA method has good specificity and high sensitivity, special apparatuses are not needed, and the method has important application values in the fields of disease monitoring, clinic diagnosis, foodstuff safety monitoring, and the like.

The invention discloses a kit, a primer pair, a probe and a method for detecting vibrio parahaemolyticus. The kit comprises an RPA reaction system which comprises an RPA primer probe mixture, the RPAprimer probe mixture comprises the primer pair and the probe, a nucleotide sequence of an upstream primer in the primer pair is shown as SEQ ID NO.1 while that of a downstream primer in the primer pair is shown as SEQ ID NO.2, and nucleotide sequences of the probe are shown as SEQ ID NO.3 and SEQ ID NO.4. The primer is high in specificity and sensitivity and accurate in detection result. By combining RPA technology for detecting vibrio parahaemolyticus with a colloidal gold flow measuring chromatography test paper strip, quick detection of vibrio parahaemolyticus can be realized, and 10pg of vibrio parahaemolyticus DNA can be detected at least.

The invention provides an LAMP primer for detecting peach venturia and a detection kit. The sequence of the LAMP primer is shown as Seq ID No.1-4. According to the LAMP primer and the detection kit, aloop-mediated isothermal amplification technology is adopted to rapidly detect the peach venturia and can accurately detect the peach venturia from complex pathogenic bacterium environments such as pathogenic plant tissue. The LAMP primer can detect propagules in various forms, such as hyphae and spores, of the peach venturia, has great significance on the aspects such as early warning of epidemic situation and pathogenic surveillance of affected areas of the peach venturia, can also avoid high investment in instruments and is convenient to apply and popularize in grass-roots units.

The invention discloses a method for extracting ganoderma lucidum sporocarp polysaccharide through fermentation pretreatment. The method comprises the following steps of: S1, fermentation strain preparation; S2, ganoderma lucidum sporocarp pretreatment; s3, ganoderma lucidum powder pretreatment; S4, liquid state fermentation or solid state fermentation; and S5, fermentation product extraction. With the method adopted, the polysaccharide content and the polysaccharide extraction rate of a ganoderma lucidum extract can be improved, the ganoderma lucidum extraction is facilitated after the fermentation pretreatment, the subsequent extraction times can be reduced, and the extraction utilization rate of the ganoderma lucidum is improved.

The invention relates to a cultivation and seedling growing matrix for melons and vegetables. The matrix is formed by uniform mixing of the following raw materials by weight: 10-25% of urea formaldehyde resin foam particles, 15-35% of fermented two-mushroom residue, and 30-55% of peat. The preparation method of the fermented two-mushroom residue includes: mixing agaricus bisporus residue with flammulina velutiper residue according to 1:(0.7-1.2), conducting windrow fermentation, when the temperature reaches above 65DEG C, conducting pile-turning once every day, and when water decreases to less than 40%, conducting sieving by a 5mm sieve, thus obtaining fluffy fermented two-mushroom residue. The urea formaldehyde resin foam involved in the invention is granular and has small particle size, is convenient for water retention and air permeability, is beneficial to rooting and fast sprouting, and also puts an end to the use of perlite and vermiculite, reduces the dust harmful to the human body in production, and has pH of 5.5, and after uniform mixing with the fully fermented two-mushroom residue, the pH is about 6.5. By using the above three raw materials, discharge of solid waste is reduced. As the urea formaldehyde resin foam particle production involves certain formaldehyde, various pathogens in the seedling growing and cultivation matrix can be effectively killed, thus avoiding the investment of bactericide before use by a user.

A storage box for print screen units of the present invention has a structure of comprising: a main frame (10) having glass doors (11) provided at the front opening thereof; slot shelves (20) having aplurality of slot parts (21) such that every print screen unit (1) is respectively erected thereon and stored and released; a plurality of slot display lamps (40) sensing and displaying whether the print screen units (1) are stored or released and whether the same are abnormally stored, while being individually lit in each of the plurality of slot parts (21); and a storing and releasing terminal(60) for controlling whether the print screen units (1) are stored and released. Therefore, when the print screen units (1) of different sizes are erected and inserted regardless of order, the print screen units (1) can be aligned and stored in a single file while the front parts thereof are matched without touching each other, and damage caused by collision and life reduction can be prevented and, simultaneously, the print screen units (1) can be protected from dust and static electricity. In addition, by means of the storing and releasing terminal (60), storing and releasing and abnormal storing can be visually and more quickly confirmed according to various color changes of the slot display lamps (40) individually provided in every slot part (21), while the print screen units (1) have aspecified position so as to be respectively stored in and released from the plurality of slot parts (21) of the storage box.

The invention discloses RPA primers and probes for rapidly detecting heterodera filipjevi, a kit and application, and belongs to the field of detection of plant parasitic nematodes. The RPA primer is as shown in SEQ ID NO: 1-2, and the probe is as shown in SEQ ID NO: 3. The RPA detection method for Heterodera filipjevi is established by using the RPA primer and the probe, the method is high in sensitivity and very strong in specificity, the detection threshold is 10-3 second-stage larva DNA, 10-4 single cyst DNA and 0.01 ng genome DNA, and the sensitivity is 100 times that of common PCR detection; no cross reaction exists between the nematodes and common closely related plant parasitic nematodes. The whole RPA detection process can be completed within one hour. The detection method is strong in specificity, high in sensitivity, visual in result, rapid and accurate, and has very high application value in the aspects of early diagnosis, field detection and early warning and the like of the Heterosporidium filipjevi disease.

The invention relates to a bipolar membrane electrodialysis cyclization method for ortho-dihalogenated carboxylic acid, belonging to the field of industrial sewage treatment. The bipolar membrane electrodialysis cyclization method for ortho-dihalogenated carboxylic acid comprises the following steps: continuously increasing a pH value in virtue of dissociated hydroxide ions in an alkali liquor chamber of a first bipolar membrane device (a two-chamber bipolar membrane device) so as to allow an ortho-dihalogenated carboxylic acid solution to undergo a cyclization reaction in the alkali liquor chamber with a pH value of 10-13 and to remove halogen, thereby obtaining cyclized carboxylate; and allowing the cyclized carboxylate to pass through a cation exchange membrane in a second bipolar membrane device (a two-chamber bipolar membrane device) so as to obtain sodium hydroxide and to obtain cyclized carboxylic acid when a pH value in an acid chamber decreases to 0.5 or less. Therefore, thebipolar membrane electrodialysis cyclization method for ortho-dihalogenated carboxylic acid in the invention effectively eliminates the addition of acid and alkali, reduces cost and pollution, and cansignificantly improve economic benefits.

The invention relates to a power system energy efficiency public service cloud platform based on cloud computing. A three-layer framework is adopted in the energy efficiency public service cloud platform and comprises an energy efficiency service Iaas layer, an energy efficiency service Paas layer and an energy efficiency service application Saas layer. The energy efficiency service Iaas layer is an infrastructure level service layer and is used for providing virtual resources, mapped through the virtual technology, for a network, a server and database physical facilities in the cloud platform. The energy efficiency service Paas layer is a platform level service layer and used for providing the execution environment of cloud middleware resources, a distributed parallel platform and application service software for power consumers. The energy efficiency service application Saas layer is a software level service layer and used for providing software services of energy efficiency services based on Web access for users. According to the energy efficiency public service cloud platform, acquisition and monitoring of energy efficiency information and diagnosis and analysis of user energy efficiency parameters are achieved through the energy efficiency service application Saas layer. By means of the framework mode, management objects and function requirements can be quickly expanded and upgraded, and user energy management efficiency is improved.

The invention discloses a method for preparing biodiesel in a eutectic system. The biodiesel is prepared by stirring and heating plant oil and methyl alcohol, which are used as raw materials, under normal pressure. The method has the advantages that a sodium hydroxide catalyst is adopted, so that the cost is low, the catalysis activity is high, and the using amount is small; the reaction conditions are mild, and the reaction can be executed under normal pressure and methyl alcohol backflow temperature conditions; a eutectic solvent consists of choline chloride and glycerinum, so that an emulsification phenomenon is avoided, and the technical flow is simplified; the product is high in purity and contains few impurities; the eutectic catalysis system consisting of the eutectic solvent and the sodium hydroxide is easy to recycle and use and has an environment-friendly effect.

The invention discloses an electric-control hydraulic special electric pole clamp which comprises clamping mechanisms, a rotary bearing, a swing joint and a main rack. The clamping mechanisms are arranged on the side portions of the two ends of the main rack, and the rotary bearing is fixed to the outer wall of the upper surface of the middle of the main rack. A cycloid motor for driving the rotary bearing to move forwards and backwards is fixed to the outer side of the middle of the main rack. A rotary connector is arranged at the center of the rotary bearing in a sleeved mode, and the lower end of the rotary connector is fixed to the main rack. The swing joint is fixed to the upper end of the rotary connector, an electromagnetic valve set is fixed in the swing joint, and the output end of the electromagnetic valve set is communicated with an oil inlet of the cycloid motor through an oil pipe. The electric pole clamp has high compatibility, and the electric pole installation convenience and efficiency are effectively improved.

The invention discloses a multi-RPA primer and probe for simultaneously detecting a newcastle disease virus, an infectious bronchitis virus and an H9N2-subtype avian influenza virus. The multi-RPA primer and probe comprise a primer and probe for detecting the newcastle disease virus, a primer and probe for detecting the infectious bronchitis virus and a primer and probe for detecting the H9N2-subtype avian influenza virus. The multi-RPA primer and probe are high in specificity, high in sensitivity and accurate in detection result. The invention further discloses a method for simultaneously detecting the newcastle disease virus, the infectious bronchitis virus and the H9N2-subtype avian influenza virus through multi-RPA. The method comprises the steps of primer synthesis, template RNA extraction, reverse transcription, multi-PCR amplification and amplification product analysis. The detection method is simple in operation and good in stability, and a low-cost, fast and specific field diagnosis method is provided for effective detection and identification of mixed infection of common avian respiratory diseases.

The invention discloses a UHPC steel-concrete composite beam full-section incremental launching comprehensive construction method. The method comprises the following steps that 10, a manufacturing site, an assembling site, an assembling and overlapping area and a cast-in-place maintenance area are arranged; 20, in the manufacturing site, bridge deck slabs and steel edge box blocks are machined and pre-matched; 30, the steel edge box block bodies are continuously matched to manufacture the steel edge box in the assembling site; 40, in the assembling and overlapping area, cross beams, small longitudinal beams and the steel edge boxes are spliced to manufacture steel beams, and the bridge deck slabs and the steel beams are overlapped; 50, in the cast-in-place maintenance area, a bridge deck slab wet joint is poured and maintained; and 60, incremental launching construction is carried out on the bridge deck slabs in the round until the incremental launching construction of the whole main bridge is finished. According to the UHPC steel-concrete composite beam full-section incremental launching comprehensive construction method, steel-concrete composite beam full-section incremental launching construction is adopted, welding operation during front-field installation of the main beam is reduced, and the overall construction quality is improved.

The invention discloses a kit, a primer pair, a probe and a method for detecting vibrio parahaemolyticus. The kit comprises an RPA reaction system which comprises an RPA primer probe mixture, the RPAprimer probe mixture comprises the primer pair and the probe, a nucleotide sequence of an upstream primer in the primer pair is shown as SEQ ID NO.1 while that of a downstream primer in the primer pair is shown as SEQ ID NO.2, and nucleotide sequences of the probe are shown as SEQ ID NO.3 and SEQ ID NO.4. The primer is high in specificity and sensitivity and accurate in detection result. By combining RPA technology for detecting vibrio parahaemolyticus with a colloidal gold flow measuring chromatography test paper strip, quick detection of vibrio parahaemolyticus can be realized, and 10pg of vibrio parahaemolyticus DNA can be detected at least.

The invention discloses an interplanting method of fast-growing eucalyptus and cunninghamia lanceolata. The interplanting method includes planting steps: (1), selecting a parcel with a thick soil layer without waterlogging; (2), transplanting: from February to May, digging holes and planting fast-growing encalyptus according to planting space of (1.5-2)mx(5-8)m, and digging holes and planting a row of cunninghamia lanceolata among row space of fast-growing encalyptus according to row space of 2-3m; (3), topdressing fast-growing encalyptus from February to April of each year; pruning and trimming parts below 1m after fast-growing encalyptus grows to 2m in height; applying decomposed fertilizer to cunninghamia lanceolata once a year according to 20-40kg per plant, ditching at positions of 0.5-1m distant from trunks during applying, applying the fertilizer into ditches, and covering with soil after fertilizing; (4), continuously felling fast-growing encalyptus according to needs after thefourth year of planting, felling according to needs after the fifth year of planting of cunninghamia lanceolata, digging up roots poor in germination according to germination conditions, and plantingin seedlings. By the method, the problem of water and soil loss caused by planting of fast-growing encalyptus can be solved effectively, and labor input for weeding and disease and pest of cunninghamia lanceolata can be reduced.

The invention provides an LAMP primer for detecting Phytophthora melonis and a kit containing the LAMP primer. The primer comprises FIP, BIP, F3 and B3 (as shown in Seq ID No.1-4). According to the invention, an LAMP primer constant-temperature amplification technology is used for the fast detection of Phytophthora melonis pathogens, thus the Phytophthora melonis can be accurately detected from complicated pathogenic bacteria contained in pathogenetic plant tissues and soil. Compared with the conventional PCR (Polymerase Chain Reaction) method, the method provided by the invention has higher specificity and sensitivity, can be used for detecting propagules, such as hypha, oospore and zoospore which have various forms, of the Phytophthora melonis, has improtant significance in the aspects of the early prewarning of a Phytophthora melonis epidemic situation, the pathogeny monitoring of an epidemic area and the like, and is convenient to popularize and use on a grass root because large instrument investment is avoided.

The invention relates to the technical field of centrifugal pumps, in particular to the water inlet and inlet and outlet base of a multistage centrifugal pump and a manufacturing method of the water inlet and inlet and outlet base. The water inlet and inlet and outlet base is mainly structurally characterized in that a water inlet and a water outlet are formed in the side wall of an shell, an inner cylinder is welded into the cavity of the shell, a water inlet corresponding to the water inlet of the shell in position is formed in the side wall of the inner cylinder, an opening is formed at the upper end of each of the shell and the inner cylinder, a shaft hole is formed in the middle of the bottom of the inner cylinder, a water inlet pipe is welded and fixed on the water inlet holes of the shell and the inner cylinder, a water outlet pipe is welded and fixed on the water outlet of the shell, and a water passing gap is formed between the shell and the side wall of the inner cylinder. The water inlet and inlet and outlet base has the advantages that the water inlet and inlet and outlet base is environmental friendly and relatively extremely low in cost, precious resources are saved, and investment in manpower and financial resources is reduced greatly.

The invention discloses a district file transmission method and system covering multicast. All authoritative servers serving the same district form an overlay network, and a new district file transmission system is obtained by increasing the forwarding function of district files on each server. The system It can realize the function of multicast transmission of zone files from the master server to other slave servers. The transmission method of the overlay multicast zone file of the present invention is: the direct transmission between the original DNS primary server and the DNS secondary server becomes the indirect transmission with the DNS secondary server as the relay node; a forwarding scheduling server is added to collect The link information between each authoritative server is calculated to obtain a multicast tree through a certain multicast tree generation algorithm, and the respective forwarding tables are also calculated for each authoritative server. Because the method of the present invention utilizes the link between the secondary servers for data transmission, the zone file transmission is accelerated. Simultaneously, the present invention can save funds, and is convenient for realization and popularization.

The invention discloses an air-passage multi-tube spiral device and process for preparing gas through dry distillation and pyrolysis on biomass. The device consists of a biomass raw material pre-treatment system, a biomass dry distillation and pyrolysis system, a gas and carbon powder separating and purifying system, a gas conveying and storing system, a carbon powder cooling and collecting system and an afterheat recycling system, wherein the biomass dry distillation and pyrolysis system is provided with a plurality of rows of dry distillation and pyrolysis sleeves; spiral material conveyingdevices and exhaust passages are arranged in each row of dry distillation and pyrolysis sleeves; and a communication pipe is arranged between adjacent dry distillation and pyrolysis sleeves, so the rows of dry distillation and pyrolysis sleeves are connected into an S-shaped pipe in series. The process comprises the following steps of: 1, crushing, drying and conveying a biomass raw material; 2, performing dry distillation and pyrolysis on the biomass; 3, separating and purifying gas and carbon powder; and 4, storing and conveying the gas. The device and the process have the beneficial effects that: the condition the gas flies around to affect the normal production is not caused, mass industrial production can be realized, the production cost is reduced, and low energy consumption is realized.

The invention provides loop-mediated isothermal amplification (LAMP) primers for detecting Phytophthora capsici and a kit comprising the primers. The primers comprise FIP, BIP, F3 and B3 (shown as Seq ID No.1-4). An LAMP primer technology is used for quickly detecting the Phytophthora capsici, and the Phytophthora capsici can be accurately detected in a complicated pathogenic bacteria environment in diseased plant tissues and soil. The method has higher specificity and sensitivity than the conventional PCR method, propagules of the Phytophthora capsici in various forms, such as mycelia, oospores and zoospores can be detected, and the method has significance in aspects of early warning of Phytophthora capsici epidemics and monitoring pathogeny in epidemic areas; meanwhile, expensive instruments can be avoided, and the method is easily promoted and used in basic level.

The invention belongs to the technical field of mineral processing and purification of graphite, and in particular relates to a method for mineral processing and purification of microcrystalline graphite. The present invention adopts one stage of coarse crushing, one stage of fine crushing, one stage or two stages of dry ultrafine crushing-classification, and then combines multi-stage beneficiation and multi-stage sweeping processes. The invention is a purification process with less grinding and more flotation, which can obtain microcrystalline graphite flotation concentrate with fixed carbon content of more than 90%, microcrystalline graphite flotation concentrate with fixed carbon content of more than 75% and fixed carbon content of less than 20% microcrystalline graphite tailings.

The invention provides an LAMP primer for detecting Phytophthora melonis and a kit containing the LAMP primer. The primer comprises FIP, BIP, F3 and B3 (as shown in Seq ID No.1-4). According to the invention, an LAMP primer constant-temperature amplification technology is used for the fast detection of Phytophthora melonis pathogens, thus the Phytophthora melonis can be accurately detected from complicated pathogenic bacteria contained in pathogenetic plant tissues and soil. Compared with the conventional PCR (Polymerase Chain Reaction) method, the method provided by the invention has higher specificity and sensitivity, can be used for detecting propagules, such as hypha, oospore and zoospore which have various forms, of the Phytophthora melonis, has improtant significance in the aspects of the early prewarning of a Phytophthora melonis epidemic situation, the pathogeny monitoring of an epidemic area and the like, and is convenient to popularize and use on a grass root because large instrument investment is avoided.

The invention discloses a leakage protector antitheft system which comprises a signal emission part, a leakage protector, a first protection circuit and a second protection circuit. The signal emission part is carried by a personnel and provided with a first wireless module, the leakage protector comprises a coil and a knife switch, the second protection circuit comprises a detection module, a signal input circuit and a signal output circuit, the signal input circuit and the signal output circuit are connected with the detection module, the signal output circuit is electrically connected withthe coil of the leakage protector, the signal output circuit controls the coil to be powered on when a secondary terminal of the knife switch is powdered on, the detection module is communicated witha second power supply when the coil is powered on, the first protection circuit comprises a main control module and a second wireless module connected with the main control module, and the signal output circuit controls the coil to be powered on when the second wireless module is connected with the first wireless module in a signal manner. Protective appliances and power supply safety of commercial power places are ensured, stolen property loss is removed, and safety problems caused by damaged power leakage protector are solved.