The invention discloses a cultivation material composition for producing high-quality agaricus blazei and a manure-free rice straw raw material cultivation method. The invention is characterized in that: the cultivation material composition comprises the following raw materials in part by weight: 1,000 to 1,500 parts of rice straw, 33 to 45 parts of ammonium bicarbonate, 35 to 45 parts of calciumsuperphosphate, 35 to 40 parts of gypsum and 34 to 50 parts of lime; and the rice straw raw material cultivation method comprises: raw material preparation and processing, namely primary fermentationof windrow, mushroom house selection and disinfection, secondary fermentation of culture materials on beds in the house, inoculation and culture, earthing and fruiting management, wherein the step ofraw material processing, namely primary fermentation, also comprises special pre-piling, pile building and pile turning. The method helps to effectively solve the problem that the content of heavy metals such as cadmium in the agaricus blazei exceeds a standard. Ammonium bicarbonate is used as a nitrogen source fertilizer in the cultivation materials, so damage caused by ammonia is prevented; theprocess is simple; the management is work-saving and labor-saving; the anlages of the agaricus blazei mushrooms is uniform and the mushroom shape is desirable; the yield and quality of the agaricus blazei mushrooms are high; and the artificial cultivation scale of the agaricus blazei can be enlarged.