The invention discloses an automatic seed sampling machine. The automatic seed sampling machine comprises an automatic seed arranger, a seed conveying device, a machine vision working position, a seed gripper group, a seed gripper group moving device, a laser cutting device, a sample disc moving device, a sample disc conveying and charging-discharging device and a seed disc conveying device, wherein the automatic seed arranger is arranged at the left side of the seed conveying device in a connecting manner; the machine vision working position is arranged at one end, close to the automatic seed arranger, of the seed conveying device; the seed gripper group moving device is arranged above the seed conveying device and is provided with the seed gripper group; the laser cutting device is arranged at one side of the seed conveying device in the moving range of the seed gripper group; and the sample disc moving device, the sample disc conveying and charging-discharging device and the seed disc conveying device are arranged at the downstream positions of the seed conveying device in sequence and in the connecting manner. The automatic seed sampling machine disclosed by the invention has the advantages of wide seed adaptability, slight damage to seeds and relatively high working efficiency.