The invention relates to a method for constructing and identifying
molecular marker fingerprint chromatogram of
Dendrobium huoshanense and similar species thereof, which solves the problems that a method of identifying
medicinal plants in the prior art has high cost, complicated procedures and long time consumption. The method comprises the steps of: 1, the collection of
Dendrobium huoshanense samples; 2 the extraction and purification of
DNA of genomes of the samples; 3. ISSR-PCR amplification; 4,
agarose gel electrophoresis; 4, the construction of ISSR
molecular marker fingerprint chromatogram of the samples to be tested; and 5, the identification of
Dendrobium huoshanense
germplasm by utilizing the constructed ISSR
molecular marker fingerprint chromatogram. The method has the advantages that the method saves the time and the cost, and can obtain an accurate and reliable identification chromatogram through the extraction to
DNA of Dendrobium plants, the ISSR-PCR amplification, and the
agarose gel electrophoresis; the method has the advantages of simplicity, convenience, quickness, good repetitiveness, and
high resolution on the identification of raw materials which are easy to confuse in appearance; and the method can identify in a
seedling stage, which has important effect on ensuring the accuracy and stability of base resources of medicinal materials.