The invention discloses a thin-layer identification method for a compound
traditional Chinese medicine preparation containing liquorice, and belongs to the technical field of thin-layer
chromatography. The technical problem to be solved is to provide a thin-layer identification method capable of simultaneously identifying the
white mulberry root-
bark, the cortex lycii radicis and the liquorice, and the thin-layer identification method is less in sampling and high in detection efficiency. According to the technical scheme, the thin-layer identification method for the liquorice-containing compound
traditional Chinese medicine preparation is characterized by comprising the following steps of: spotting a
test solution and a reference solution on a thin-layer plate, respectively spotting the
test solution and the reference solution on the same
silica gel G thin-layer plate to form a strip shape, taking a
butyl acetate-
methanol-
acetone-water-
formic acid solution as a developing
solvent, and carrying out thin-layer
chromatography on the
test solution and the reference solution to obtain the liquorice-containing compound
traditional Chinese medicine preparation. The volume ratio of
butyl acetate to
methanol to
acetone to water to
formic acid is 5: (0.5-2): (2-3): (0.5-1): 1, expanding, taking out, airing, spraying a 10%
sulfuric acid ethanol solution of 2%
vanillin, heating until the color of spots is clear, and inspecting under
sunlight.