Construction and identification method of molecular marking fingerprint of Dendrobium huoshanense and similitude species thereof
A Dendrobium Huoshanense, fingerprinting technology, applied in the construction and identification of Dendrobium germplasm fingerprints, can solve the problems of high cost, long time-consuming, complicated procedures, etc., and achieve simple steps, optimized reaction system, and strong genotyping ability Effect
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Embodiment 1
[0037] In this example, the collected leaves of 8 Dendrobium strains that are similar to Dendrobium Huoshanense in terms of medicinal value and morphology are used as test materials, the total DNA of the genome is extracted, and the ISSR molecular marker analysis of the specified primers is carried out. The presented bands established ISSR-PCR molecular marker fingerprints of each test sample, and the constructed ISSR-PCR molecular marker fingerprints were used for germplasm identification of Dendrobium.
[0038] The specific operation steps are as follows:
[0039] 1), collecting fresh dendrobium sample pretreatment;
[0040] Consume starch with fresh Dendrobium dendrobium more than 6 hours;
[0041] Take 10 grams of fresh dendrobium leaves after dark treatment, put more than 50 grams of acetone and water mixed solution with a ratio of 3:1 and store for two days, take out the materials, soak in more than 50 grams of distilled water for one hour, and then take out the materia...
Embodiment 2
[0082] Identification of Dendrobium Huoshanenseensis by the DNA Fingerprints of Eight Dendrobium Lines Constructed
[0083] Get the unknown sample to establish fingerprints according to the method in Example 1, compare the fingerprints of the unknown samples with the obtained DNA fingerprints of the 8 strains of Dendrobium, and if it is the same as the Dendrobium Huoshanense spectrum, it is Dendrobium Huoshanense, otherwise it is not Dendrobium Huoshanense.
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