The invention discloses an
exosome detection method for Alzheimer's
disease, and relates to
exosome separation and extraction. The method comprises the following steps: modifying the surface of a biological sensing
chip, and forming an S-Au bond by utilizing MUA and a
gold film to construct a self-assembled monomolecular layer rich in carboxyl; activating carboxyl by using an EDC-NHS solution, anchoring an
antibody capable of recognizing
exosome membrane protein secreted by brain-derived neuronal cells, and closing non-specific sites by using
bovine serum albumin; a serum sample and a
chip are incubated, exosomes are separated through a
glycine buffer solution with the pH value being 2.0, the pH value is adjusted to be neutral, and a
lysis solution containing 1% of Triton X-100 is added for
lysis; and finally, mounting an integrated
optical fiber probe to measure a
reflection spectrum and monitor
resonance wavelength, and realizing quantitative detection by combining multi-marker
conjoint analysis. The exosome can be enriched and purified with high purity and high specificity, the process is simple, the detection sensitivity is high, and the AD detection accuracy is remarkably improved. The process is simple, complex operations such as ultracentrifugation are avoided, and the detection time is shortened.