Porphyra haitanensis germ plasma identification fingerprint construction method and its uses

A technology of fingerprint spectrum and construction method, applied in the field of fingerprint spectrum construction

Inactive Publication Date: 2008-02-20
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] SRAP—Related Sequence Amplified Polymorphism Marker is a new type of molecular marker technology with high polymorphism, medium yield, good repeatability, simple operation, uniform distribution in the genome, and easier detection of amplified The target fragment is sequenced, and the primers are versatile, and the forward primer can be combined with the reverse primer in pairs, and multiple primer pairs can be assembled with a small number of primers, thereby improving the efficiency of prim...

Method used

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  • Porphyra haitanensis germ plasma identification fingerprint construction method and its uses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] In this example, the collected thallus of 15 Porphyra laver strains that are widely representative in production or research are used as test materials, the total DNA of the genome is extracted, the SRAP molecular marker analysis of the specified primers is carried out, and the representative ones are selected. Polymorphic bands, establish their DNA fingerprints, and convert the fingerprints into digital fingerprints composed of "0" and "l", input the fingerprint recognition software to establish the DNA digital fingerprint library of almond laver, and finally construct the The DNA digital fingerprint library was used in the germplasm identification of Porphyra alba. The specific implementation method is as follows:

[0034] 1. Selected experimental materials:

[0035] Select the Porphyra laver line that is widely used in partial production or research, as shown in Table 1, the Porphyra laver strain that is widely used in production or research that has been collected ...

Embodiment 2

[0072] Apply the altar laver digital fingerprint library constructed in the present invention to the germplasm identification of altar laver:

[0073] Assuming that it is necessary to determine which algae strain a batch of almond laver belongs to which has been used on a large scale in production, this batch of altar laver can be identified according to the following method, and finally determine which strain it belongs to.

[0074] First, the genomic DNA was extracted from this batch of laver according to the method in Example 1, and purified as a template, using ME1: 5'-TAGGTCCAAACCGGATA-3' as the forward primer, and EM9: 5'-GACTGCGTACGAATTAAC-3' as the reverse primer , using the SRAP amplification experimental system for SRAP amplification, the amplified product was electrophoresed on 8% polyacrylamide gel at 60W constant power for 1.5h, and was visualized by silver staining, according to the selected 6 polymorphic bands Draw the DNA fingerprints of each sample of this bat...

Embodiment 3

[0077] In this embodiment, 21 Porphyra laver strains (YSI~YSIV, YSV-①~YSV-③, YSVI, YSVII-①~YSVII-⑤, YSX-①~YSX-⑤ and YSXI~YSXIII) thallus as the test material, extract the total DNA of the genome, carry out SRAP molecular marker analysis with specified primers, select representative polymorphic bands, establish their DNA fingerprints, and convert the fingerprints into For the digital fingerprint composed of "0" and "1", input the fingerprint recognition software to build the DNA digital fingerprint library of Porphyra alba, and finally use the constructed DNA digital fingerprint library in the germplasm identification of Porphyra almanac. The specific implementation method is as follows:

[0078] 2. Selected experimental materials:

[0079] Twenty-one Porphyra laver strains (YSI~YSIV, YSV-①~YSV-③, YSVI, YSVII-①~YSVII-⑤, YSX-①~YSX-⑤ and YSXI~YSXIII) in the Fujian Porphyra Germplasm Bank were used. ) fronds.

[0080] 2. Extraction and purification of Porphyra japonica genomic ...

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Abstract

The utility model relates to a reconstruction method and application for identifying fingerprint chromatogram of porphyra haitaensis, belonging to fingerprint chromatogram of porphyra haitaensis, in particular to reconstruction method and application for identifying fingerprint chromatogram of porphyra haitaensis. The utility model provides a rapid accurate reconstruction method for identifying fingerprint chromatogram of porphyra haitaensis and the application for realizing the porphyra haitaensis identification automation, which is characterized in that porphyra haitaensis specimens are collected; genome DNA of various specimens is refined and purified; the PCR is enlarged, the refined genome DNA is utilized as a template, SRAP molecule marking analysis is performed by the optimized experimental system; the representative SRAP multi-state bars are selected for reconstructing the DNA fingerprint chromatogram of various specimens; the reconstructed DNA fingerprint chromatogram is converted to the identifiable digital fingerprint by computer, and then is inputted to the fingerprint chromatogram identification software, thereby the digital fingerprint database of porphyra haitaensis is established. The utility model is used for follow-up porphyra haitaensis automation identification.

Description

technical field [0001] The invention relates to a fingerprint of Porphyra alba, in particular to a method for constructing a fingerprint of Porphyra germplasm identification and its application. Background technique [0002] Porphyra haitanensis is a unique species of warm temperate zone in my country. It is an important economic red algae artificially cultured in coastal areas of Fujian, Zhejiang and Guangdong. Its output accounts for about 80% of the total national laver production. In the past, the classification of Porphyra mainly relied on morphological methods. However, because most morphological characters are greatly affected by environmental factors, the descriptive characteristics are limited, and it is difficult to meet the requirements of Porphyra germplasm identification and improved breeding. The emergence of molecular marker technology has brought an opportunity to solve this problem. It directly marks and detects the genetic variation of the genome at the DNA...

Claims

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Application Information

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IPC IPC(8): G01N30/00G01N1/28C12Q1/68
Inventor 陈昌生谢潮添纪德华徐燕
Owner JIMEI UNIV
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