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Detection method applied to origin tracing based on IKnife-REIMS technology

A technology of origin traceability and detection method, which is applied in the preparation of test samples, measuring devices, and material analysis through electromagnetic means, which can solve the problems of no traceability of origin and achieve stable results and shorten analysis time

Pending Publication Date: 2022-02-18
ZUNYI INST OF PROD QUALITY INSPECTION & TESTING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is that there is currently no method for tracing the origin of Xishui Ma sheep. The present invention provides a REIMS-based ionization source that does not require sample pretreatment, collects a large amount of data through a time-of-flight mass spectrometer, and Live The combination of ID software model algorithm and real-time traceability system technology is applied to the real-time traceability detection method of the origin, which solves the above-mentioned problems well

Method used

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  • Detection method applied to origin tracing based on IKnife-REIMS technology
  • Detection method applied to origin tracing based on IKnife-REIMS technology
  • Detection method applied to origin tracing based on IKnife-REIMS technology

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Effect test

Embodiment 1

[0024] 1. Materials and Instruments

[0025] Pure methanol mass spectrometry, Merck, Germany; leucine enkephalin: Leu-Enkaphalin, Waters, USA; frozen dumpling box, food-grade PP material, with a sealing cover, the size is 31cm×23.5cm×3.5cm, each The grid size is 7cm×3.5cm, and a total of 62 groups of reliable samples have been collected. The samples are collected by special personnel to ensure that the origin is accurate and true. The details of the tested samples are shown in Table 1.

[0026] Table 1 Sample source information table

[0027]

[0028] In-situ ionization source time-of-flight mass spectrometer: Xevo G2-XS REMIS Q-TOF: Waters Company of the United States; high-frequency electronic knife: product of Aiboerbe Company of Germany; XY-201 smoke purifier: Shenzhen Xingyi Environmental Protection Technology Co., Ltd.; Milli-QReference ultra-pure water generator: Millipore Company of the United States; IKnife-REIMS The diagram of the mutton sample collection device...

Embodiment 2

[0039] 1. Optimization results of acquisition conditions

[0040] For fresh mutton samples with more lipids, such as epidermis and subcutaneous fat, the signal response of cutting is low, and it is quite different from the required experimental data, so only the lean tissue part was collected. In order to ensure the stability and validity of the collected data, the intensity of each data peak in the total ion current diagram should not be lower than 5×10 7 , so through repeated testing of different parameter conditions, it was finally determined that the voltage was set to 40V, the length was 2cm, the incision depth was 0.5cm, and the cutting duration was 2-3s as the optimal acquisition conditions. The total ion chromatogram and mass spectrogram obtained by cutting the tenderloin of Xishui Ma sheep in negative ion mode are shown in the appendix figure 2 And attached image 3 .

[0041] 2. Cluster analysis results

[0042] Multivariate statistical analysis was applied to i...

Embodiment 3

[0044] 1. Identification model validation

[0045] In the Live ID software, a total of 709 data of 62 samples of Xishui Ma sheep, Inner Mongolia sheep, Jilin sheep and Guizhou sheep were analyzed by the overall cross-model inter-group validation analysis, randomly divided into 5 groups, and 4 groups were randomly selected to establish a new model. Model, the model is verified on the remaining 1 set of data, repeated 5 times, and the measurement accuracy of the prediction model is predicted. The recognition rate is 97.32%, and the results are listed in Table 2 and Figure 4 .

[0046] Table 2 Model cross-validation results

[0047]

[0048] Since the establishment of the model is to identify the origin traceability of Xishui Ma sheep in the production process and circulation field, the experimenters randomly selected authentic samples of Xishui Ma sheep in the market and did not add them to the establishment of the model. Through the Iknife-REIMS system The sample was rep...

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Abstract

The invention discloses a method for rapidly identifying Shuanghuama sheep by adopting an REIMS rapid evaporation ionization mass spectrometry technology and a high-resolution mass spectrometry. The real-time source-tracing detection method for the Shuanghuama sheep is obtained by on-site collection of mutton samples from different producing areas, instrument acquisition parameter optimization and model establishment. Recognition accuracy of the method is 97.81%; differential marker analysis is conducted on the data by using principal component analysis and a partial least square discriminant model, and a series of characteristic compounds with large differences are identified. The method does not need pretreatment, and is short in detection time, high in recognition rate and stable in result.

Description

technical field [0001] The invention belongs to the field of origin traceability, and in particular relates to a detection method for origin traceability of Xishui Ma sheep based on IKnife-REIMS. Background technique [0002] Xishui Ma sheep is a local fine breed in the country. Xishui County, Zunyi City, Guizhou Province is the place of origin. Now it has become a product of China's national geographical indication. It is an important animal genetic resource in my country. In 2009, it was identified as a national livestock and poultry genetic resource by experts from the National Animal Genetic Resources Committee. In order to expand and strengthen the mutton sheep industry, the local government and enterprises combine the excellent quality resources of Xishui Ma sheep to develop special sheep products, establish a brand, and meet the needs of the market and consumers. However, at present, mutton from other origins or varieties can be seen everywhere in the market, fake an...

Claims

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Application Information

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IPC IPC(8): G01N27/626G01N1/28
CPCG01N27/626G01N1/28
Inventor 张季向丽萍王兰兰李霞刘桂岚李梅卢叶凌蕾杨沙卢泽毅
Owner ZUNYI INST OF PROD QUALITY INSPECTION & TESTING
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