Rice seedling-stage salt-tolerant gene qST11 and molecular marker method thereof

Abstract

The invention relates to a rice seedling-stage salt-tolerant gene qST11 and a molecular marker method thereof and belongs to the fields of rice stress-resistance breeding and molecular genetics. The rice seedling-stage salt-tolerant gene qST11 is characterized in that the BC1F10 introgression line group constructed by the Zhongguangxiang No. 1 and the IR75862 as well as the F2 group constructed by cross derivation of the introgression lines are utilized and the single-marker analysis is used, so that the gene site qST11 related to the rice seedling-stage salt tolerance at the eleventh chromosome of the rice genome is positioned and a practical and economical marker RM332 which is closely linked to the gene site qST11 and is on the basis of the PCR amplification is obtained. The rice seedling-stage salt-tolerant gene qST11 is used in assisted selection breeding and polymerization breeding of rice seedling-stage salt tolerance, so that the defect of lack of salt-tolerant gene in prior art is effectively made up and the genotype selection to the low-generation breeding population in the seedling stage is realized; thus salt-tolerant individuals are obtained and timely hybrid trans-breeding is facilitated, so that the errors of phenotypic identifications during the salt tolerance identification process are reduced, the breeding efficiency is improved and the breeding process is fastened.

Description

Technical field: [0001] The invention relates to a rice seedling stage salt-tolerant gene qST11 and a molecular marking method thereof, belonging to the fields of rice stress resistance breeding and molecular genetics. Background technique: [0002] Rice is one of the most important food crops in the world. It is sensitive to salt damage, especially at the seedling stage; through molecular breeding, the aggregation of favorable genes for salt tolerance and the selection of salt-tolerant rice varieties are the most direct and effective way to improve the salt tolerance of rice. Methods. [0003] Molecular genetic studies have shown that rice salt tolerance is controlled by multiple genes. So far, researchers have used forward genetics to identify multiple QTLs that affect rice salt tolerance, among which genes such as Saltol have been fine-mapped, and SKC1 has been cloned. In addition, the researchers also cloned a number of genes affecting salt tolerance in rice, such as O...

AI Technical Summary

Problems solved by technology

In 2012, the set of introduced lines was tested for salt tolerance at the seedling stage, and it was found that although the parents Zhongguangxiang 1 and IR75862 were moderately salt-sensitive, some of the introduced lines showed strong salt-tolerant characteristics at the seedling stage

After comparison and mapping, it was found that there is no gene reporting on the level of salt damage and survival days at this locus, so qST11 is a new gene controlling the salt tolerance of rice seedlings

Images