Rice seedling-stage salt-resistant gene qST2 and molecular marking method thereof

Abstract

The invention relates to a rice seedling-stage salt-resistant gene qST2 and a molecular marking method thereof, and belongs to the field of rice stress-resistance breeding and molecular genetics. The molecular marking method is characterized by comprising the following steps: performing single marking analysis by using an F2 group generated from a BC1F10 introgression line established from Zhongguangxiang #1 and IR75862, and an introgression line in an intercross derivation manner, positioning that a second chromosome of a rice genome has a gene site qST2 related to rice seedling-stage salt-resistance, thereby obtaining a practical and economic marker RM110 which is tightly linked with the gene site qST2 and based on PCR amplification. The rice seedling-stage salt-resistant gene qST2 can be applied to assistant selective breeding and pyramiding breeding for rice seedling-stage salt-resistance, the defect that conventional rice is lack of salt resistance genes can be effectively overcome, genotypes can be selected for low-generation breeding groups can be performed in the seedling stage, salt-resistant individuals can be obtained, hybridization transformation can be conveniently performed in time, the errors of phenotype identification in salt-resistance identification process can be reduced, the breeding efficiency can be improved, and the breeding progress can be accelerated.

Description

Technical field: [0001] The invention relates to a rice seedling stage salt-tolerant gene qST2 and a molecular marker method thereof, belonging to the fields of rice stress-resistant breeding and molecular genetics. Background technique: [0002] Rice, one of the most important food crops in the world, is sensitive to salt damage, especially at the seedling stage. 2.9×10 in Asia alone 7 Hectares of salt-damaged arable land have caused great losses to rice production. Breeding salt-tolerant rice varieties is the most direct and effective way to improve rice salt tolerance. [0003] Molecular genetic studies have shown that salt tolerance in rice is a quantitative trait controlled by multiple genes. So far, several major QTLs affecting salt tolerance in rice have been identified, among which genes such as Saltol have been fine-mapped, and SKC1 has been cloned. In addition, the researchers also cloned several rice salt-tolerant genes such as OsNAP, OsSIK1, SNAC1, OsNAC10, O...

AI Technical Summary

Problems solved by technology

In 2012, the set of introduced lines was tested for salt tolerance at the seedling stage, and it was found that although the parents Zhongguangxiang 1 and IR75862 were moderately salt-sensitive, some of the introduced lines showed strong salt-tolerant characteristics at the seedling stage

After comparison and mapping, it was found that there is no gene reported to control salt-tolerance-related traits at this locus, so qST2 may be a new gene that controls salt-tolerance in rice seedlings

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