Method for detecting electrochemical luminescence

A technology of luminescence detection and electrochemistry, applied in chemiluminescence/bioluminescence, organic chemistry, electrical excitation analysis, etc., can solve the problem that the intensity of electrochemiluminescence is not the strongest, and achieve the effect of high electrochemiluminescence efficiency

Inactive Publication Date: 2009-09-16
CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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Problems solved by technology

However, the electrochemiluminescence intensity of di-n-butylethanolamine and pyridine ruthenium is not the strongest,

Method used

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  • Method for detecting electrochemical luminescence
  • Method for detecting electrochemical luminescence
  • Method for detecting electrochemical luminescence

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Experimental program
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Effect test

Embodiment 1

[0012] Embodiment 1: detection using an electrochemical workstation and a BPCL weak luminescence measuring instrument. The concentration of fixed n-butyldiethanolamine is 0.05mmol / L, the concentration of ruthenium pyridine is 1μmol / L, the buffer solution is 0.1mol / L phosphate with pH 7.5, and the gold electrode is used as the working electrode, and a step of 1.5V is applied At the same time, a luminescence detection voltage of 800V was applied to detect the luminescence signal of ruthenium pyridine, and the electrochemiluminescence detection was completed, and the average intensity of the luminescence signal obtained was 22.33.

Embodiment 2

[0013] Embodiment 2: using an electrochemical workstation and a BPCL weak luminescence measuring instrument to detect. The fixed n-butyldiethanolamine concentration is 15mmol / L, the buffer solution is 0.1mol / L phosphate at pH 7.5, add 4.0×10 -10 mol / L of the detected substance pyridine ruthenium, using a gold electrode as the working electrode, applying a step potential of 1.35V, and simultaneously applying a luminescence detection voltage of 700V, detecting the luminescence signal of pyridine ruthenium, completing the electrochemiluminescence detection, and obtaining the luminescence signal of pyridine ruthenium The average strength is 68.

Embodiment 3

[0014] Embodiment 3: detection using an electrochemical workstation and a BPCL weak luminescence measuring instrument. The concentration of fixed n-butyldiethanolamine is 1mmol / L, the concentration of ruthenium pyridinium to be tested is 1mmol / L, dissolved in 0.1mol / L phosphate buffer solution with pH 7.5, the gold electrode is used as the working electrode, and 1.5V is applied. Step potential, while applying a luminescence detection voltage of 500V, detects the luminescence signal of ruthenium pyridine, and completes the electrochemiluminescence detection. The average intensity of the luminescence signal obtained is 6751.

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Abstract

The invention relates to a method for detecting electrochemical luminescence. A luminescence system of electrochemical luminescence detection is a pyridyl ruthenium/butyl diethanolamine luminescence system. Pyridyl ruthenium and butyl diethanolamine are mixed in phosphate buffer solution with pH between 7 and 9 to be simulated to be luminescent by an electrochemical method and analyzed. The concentration range of the pyridyl ruthenium is 1*10< [-10] >to 1mmol/L. The concentration range of the butyl diethanolamine is 0.05-50mmol/L. The electrochemical luminous efficiency of the pyridyl ruthenium/butyl diethanolamine luminescence system is high, and the concentration of co-reactant butyl diethanolamine can be very low. When the concentration of the pyridyl ruthenium is 1 mumol/L, and the concentration of the butyl diethanolamine is 25mmol/L, under the same condition, the electrochemical luminous intensity of the pyridyl ruthenium/butyl diethanolamine luminescence system at a gold electrode is approximately 1.3 times stronger than the electrochemical luminous intensity of the pyridyl ruthenium/butyl diethanolamine luminescence system. The invention can be widely applied to marker analysis.

Description

technical field [0001] The invention relates to a method for electrochemiluminescence detection. Background technique [0002] Ruthenium pyridinium electrochemiluminescence analysis is a new generation labeling analysis technology. The prior art is based on the electrochemiluminescent reaction of a high concentration of tripropylamine with a lower concentration of a pyridine ruthenium label for bioanalysis. However, the ruthenium pyridine / tripropylamine electrochemiluminescent system has the following main disadvantages. First, the reaction of tripropylamine is slow, which limits the sensitivity of detection; second, a very high concentration of tripropylamine is required to achieve high-sensitivity detection; A high concentration of phosphate can make tripropylamine with a concentration as high as 100mmol / L dissolve in neutral phosphate buffer solution; finally, tripropylamine is highly volatile, has a relatively large odor and certain toxicity. References (Angew.Chem.In...

Claims

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Application Information

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IPC IPC(8): G01N21/76G01N21/69C07D213/02
Inventor 徐国宝韩双
Owner CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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