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A kind of preparation method of electrochemiluminescence immunosensor without co-reaction dosage form

An immunosensor and luminescent immunity technology, applied in the field of analysis, to achieve the effects of simplifying detection steps and processes, avoiding damage, and high selectivity

Active Publication Date: 2022-07-05
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no application technology that only relies on anodic oxygen or cathodic reduction to achieve co-reactant-free electrochemiluminescence

Method used

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  • A kind of preparation method of electrochemiluminescence immunosensor without co-reaction dosage form
  • A kind of preparation method of electrochemiluminescence immunosensor without co-reaction dosage form
  • A kind of preparation method of electrochemiluminescence immunosensor without co-reaction dosage form

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] Preparation of oil-soluble indium phosphide nanocrystals (InP NCs): put 0.34 mmol of indium bromide, 2.2 mmol of zinc bromide and 5 mL of 3 mmol / L oleylamine into a three-necked flask, mix them in a temperature-controlled heating magnetic stirrer, and then add Nitrogen, stirred at 120 °C for 1 h, removed the water and oxygen in the mixed solution until the solution was clear; continued to heat up to 200 °C, added 1 mL of tris(dimethylamino) phosphine and 1 mL of oleylamine with a concentration of 2.4 mmol / mL, and reacted for 20 min When the solution changes from colorless to red, heating is stopped to obtain an oil-soluble indium phosphide nanocrystal dispersion solution; then the obtained dispersion solution is centrifuged, and the obtained precipitate is washed with ethanol to obtain oil-soluble indium phosphide nanocrystals.

[0079] The oil-soluble indium phosphide nanocrystals were tested by fluorescence ultraviolet spectrum. figure 1 shown.

[0080] Preparation o...

Embodiment 2

[0094] The steps are the same as those in Example 1, except that the prepared indium phosphide / zinc sulfide nanocrystal solution is dropped on the copper mesh to observe the morphology. The high magnification transmission electron microscope pictures of the prepared water-soluble indium phosphide / zinc sulfide nanocrystals were tested, such as Figure 5 shown.

Embodiment 3

[0096] The steps are the same as in Example 1, the difference is that the prepared indium phosphide / zinc sulfide nanocrystal solution is drip-dried to make powder and test the X-ray diffraction pattern of the water-soluble indium phosphide / zinc sulfide nanocrystal prepared, like Image 6 shown.

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Abstract

The invention belongs to the field of analytical technology methods, and relates to a preparation method of a non-co-reaction formulation type electrochemiluminescence immunosensor, which mainly comprises (1) using indium bromide, tris(dimethylamino)phosphine, zinc bromide and oleylamine as raw materials, prepare oil-soluble indium phosphide nanocrystal dispersion solution; (2) use oil-soluble indium phosphide nanocrystals, zinc acetate and n-dodecanethiol as raw materials to prepare oil-soluble indium phosphide / zinc sulfide nanocrystals; (3) Dissolve oil-soluble indium phosphide / zinc sulfide nanocrystals in dichloromethane, add N,N-dimethylformamide, mercaptopropionic acid and thiomalic acid to prepare a water-soluble indium phosphide / zinc sulfide nanocrystal solution (4) Preparation of water-soluble indium phosphide / zinc sulfide nanocrystal-labeled secondary antibodies; (5) Preparation of non-co-reactive formulation type electrochemiluminescence immunosensors. The present invention does not need to add a co-reactant in the preparation process, thereby avoiding damage to biological substances and cells caused by the co-reactant.

Description

technical field [0001] The invention belongs to the field of analytical technology methods, and relates to a preparation method of an electrochemiluminescence immunosensor without co-reaction formulations using indium phosphide / zinc sulfide nanomaterials as markers. Background technique [0002] Electrochemiluminescence (ECL), a technique combining chemiluminescence and electrochemiluminescence, has attracted extensive attention in biological analysis, environmental monitoring, and clinical diagnosis due to its fast response, high sensitivity, low cost, and easy control. In ECL detection, the high-sensitivity detection of the target depends not only on the high luminescence efficiency of the luminophore, but also on the efficient signal switching mode. [0003] Co-reactant-type electrochemiluminescence can realize ECL by means of unidirectional anode or unidirectional cathode electrochemical driving, and has high radiation efficiency and high intensity. It is the most common...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327G01N21/76
CPCG01N27/26G01N27/30G01N27/3278G01N21/76
Inventor 邹桂征傅莉
Owner SHANDONG UNIV
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