Preparation and application of electrochemiluminescence sensor for detecting I-type procollagen amino-terminal protopeptide iridium nanocrystals
A technology of primary amino and nanocrystals, applied in the field of nanomaterials, can solve the problems of easy agglomeration, poor water solubility, and limited applications
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Embodiment 1
[0033] Embodiment 1. Preparation and application of an iridium nanocrystal electrochemiluminescence sensor for detecting type I procollagen N-terminal propeptide, the steps of the iridium nanocrystal solution are as follows:
[0034] At room temperature, 10 mg of tris[2-(p-tolyl)pyridine-C2,N]iridium Ir(mppy) 3 The solid was dissolved in 1 mL of tetrahydrofuran to prepare Ir(mppy) with a mass concentration of 10 mg / mL 3 solution, measure 0.5 mL of Ir(mppy) with a mass concentration of 10 mg / mL 3 The solution was added dropwise to 8.4 mL bovine serum albumin (BSA) solution with a mass concentration of 5 mg / mL, at 55 o After stirring continuously for 2 h at C, then add 0.1 mL of alkali solution with a molar concentration of 0.1 mol / L to adjust the solution to pH 12, at 37 o Stirring was continued at C for 12 h to obtain a dark yellow precipitate. The product was separated and purified by ultrafiltration and centrifugation, and the final iridium nanocrystals were dispersed into...
Embodiment 2
[0035] Example 2. Preparation and application of an iridium nanocrystal electrochemiluminescent sensor for detecting type I procollagen N-terminal propeptide. The preparation steps of the iridium nanocrystal solution are as follows:
[0036] At room temperature, 15 mg of tris[2-(p-tolyl)pyridine-C2, N]iridium Ir(mppy) 3 The solid was dissolved in 2 mL of tetrahydrofuran to prepare Ir(mppy) with a mass concentration of 7.5 mg / mL 3 solution, measure 1 mL of Ir(mppy) with a mass concentration of 7.5 mg / mL 3 The solution was added dropwise to 8.9 mL of bovine serum albumin BSA solution with a mass concentration of 10 mg / mL, at 55 o After stirring continuously for 3 h at C, then add 0.1 mL of alkali solution with a molar concentration of 0.1 mol / L to adjust the solution to pH 12, at 37 o Stirring was continued at C for 18 h to obtain a dark yellow precipitate. The product was separated and purified by ultrafiltration and centrifugation, and the final iridium nanocrystals were dis...
Embodiment 3
[0037] Example 3. Preparation and application of an iridium nanocrystal electrochemiluminescence sensor for detecting type I procollagen N-terminal propeptide. The preparation steps of the iridium nanocrystal solution are as follows:
[0038] At room temperature, 20 mg of tris[2-(p-tolyl)pyridine-C2,N]iridium Ir(mppy) 3The solid was dissolved in 4 mL of tetrahydrofuran to prepare Ir(mppy) with a mass concentration of 5 mg / mL 3 solution, measure 1.5 mL of Ir(mppy) with a mass concentration of 5 mg / mL 3 The solution was added dropwise to 8.4 mL of bovine serum albumin BSA solution with a mass concentration of 10 mg / mL, at 55 o After stirring continuously for 4 h at C, then add 0.1 mL of alkali solution with a molar concentration of 0.1 mol / L to adjust the solution to pH 12. o Stirring was continued at C for 24 h to obtain a dark yellow precipitate. The product was separated and purified by ultrafiltration and centrifugation, and the final iridium nanocrystals were dispersed in...
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