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A cyclometallic iridium complex, its preparation method and its application as an electrochemiluminescence marker

A technology of iridium complexes and ring metals, applied in the field of biological analysis, can solve the problems of difficult luminescence wavelength, unavailable biomolecular markers, non-reactivity, etc., to overcome single luminescence color, good economic and social benefits, and huge market applications foreground effect

Active Publication Date: 2019-05-17
SUZHOU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The currently commercialized electrochemiluminescence labeling molecules are mainly terpyridine ruthenium derivatives, but they have obvious disadvantages, such as: the luminous color is relatively single, the luminous quantum efficiency is low, and the luminous wavelength is difficult to adjust through the molecular structure.
[0003] The prior art discloses some cyclometallic compounds, which theoretically have better electrochemiluminescent properties; but they are not reactive and cannot form couplings with biomolecules, making them impractical for practical application, that is, they cannot be used for biomolecular labeling, and now Some application systems are all organic phases. Due to the loss of biological activity of biomolecules in the organic phase, it is further impracticable for practical application; in addition, the electrical properties of the compound itself have an extremely important impact on whether it can be used as a biomolecular marker and its marking effect

Method used

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  • A cyclometallic iridium complex, its preparation method and its application as an electrochemiluminescence marker
  • A cyclometallic iridium complex, its preparation method and its application as an electrochemiluminescence marker
  • A cyclometallic iridium complex, its preparation method and its application as an electrochemiluminescence marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 The structural formula of the electrochemiluminescence marker used is as follows:

[0035]

[0036] Using n-tripropylamine as a co-reactant, in PBS buffer solution, a 0 V-1 V-0 V cyclic voltammetry scan was applied on the electrode. As the scanning potential gradually increased, the electrode surface current gradually increased. - Tripropylamine is oxidized and the largest ECL signal is detected as figure 1 shown.

Embodiment 2

[0037] Example 2 The structural formula of the electrochemiluminescence marker used is as follows:

[0038]

[0039] Using n-tripropylamine as a co-reactant, in PBS buffer solution, a 0 V-1 V-0 V cyclic voltammetry scan was applied on the electrode. As the scanning potential gradually increased, the electrode surface current gradually increased. -Tripropylamine was oxidized and the largest ECL signal was detected.

Embodiment 3

[0040] Example 3 The structural formula of the electrochemiluminescence marker used is as follows:

[0041]

[0042] Using triethylamine as a co-reactant, in Tris buffer solution, a 0 V-1.2 V-0 V cyclic voltammetry scan was applied on the electrode. As the scanning potential gradually increased, the electrode surface current gradually increased. With the co-reaction The substance is oxidized, and the largest electrochemiluminescence signal is detected, as shown in the attached figure 2 shown.

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Abstract

The invention discloses cyclometalated iridium complexes, a preparation method thereof and an application of the cyclometalated iridium complexes as electrochemiluminescent markers. The cyclometalated iridium complexes have carboxylic groups and can be applied to biological molecular marking, and meanwhile, electrochemiluminescent wavelength can be adjusted from red light to blue-green light through change of a main ligand structure; according to the electrochemiluminescent markers as protein molecules, the marked protein molecules have higher electrochemiluminescent signals under the same condition compared with traditional electrochemiluminescent markers, namely, tris (bipyridine) ruthenium derivatives, and therefore, the cyclometalated iridium complexes have great application prospect in the field of electrochemiluminescent marking of the biological molecules.

Description

technical field [0001] The invention belongs to the field of biological analysis, and in particular relates to an electrochemiluminescence marker of biomolecules and an electrochemiluminescence analysis and detection of biomolecules. Background technique [0002] In the field of biological analysis and detection, electrochemiluminescence immunoassay is a new generation of micro-labeled immunoassay technology after radioimmunoassay, enzyme immunoassay, fluorescence immunoassay and chemiluminescence immunoassay. It has high detection sensitivity (up to femtomolar level), selectivity Good, fast detection time, wide linear range and other advantages, it is widely used in basic medicine, clinical application, food safety and other fields. The currently commercialized electrochemiluminescent labeling molecules are mainly terpyridine ruthenium derivatives, but they have obvious disadvantages, such as: the luminous color is relatively single, the luminous quantum efficiency is low, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07F15/00C09K11/06G01N21/76
CPCC07F15/0033C09K11/06C09K2211/185G01N21/76
Inventor 周宇扬
Owner SUZHOU UNIV OF SCI & TECH
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