Method for determining optimal SNP quantity as well as performing genome selective breeding on production performance of large yellow croakers through selection markers

Abstract

The invention discloses a method for determining optimal SNP quantity as well as performing genome selective breeding on production performance of large yellow croakers through selection markers. The method comprises the following steps: performing phenotype determination and genomic sequencing on the production performance of individuals of a reference group to obtain SNP locus; screening out the qualified SNP locus and supplementing the deleted genotype; dividing the reference group into a training set and a validation set to perform hybridization validation; screening the SNP locus most remarkably associated with the character through single marker analysis, and then calculating GEBV of individuals of the validation set only by using the locus and through a GBLUP method; further obtaining breeding value estimation accuracy under each screening SNP quantity; finally determining the optimal quantity of SNP screening; and according to the optimal quantity, calculating the GEBV by the GBLUP method, further obtaining the breeding value estimation accuracy and performing genome selective breeding according to the value size. By the method, the genomic selection cost on the production performance of the large yellow croakers can be reduced remarkably.

Description

technical field [0001] The invention relates to the field of genome selection breeding, in particular to a method for determining the optimal SNP quantity and performing genome selection breeding on the production performance of large yellow croaker through screening markers. Background technique [0002] The traditional breeding value estimation method is mainly carried out through phenotype and pedigree records, and this method is called Best Linear Unbiased Prediction (BLUP). Although the BLUP method has achieved great success in animal breeding, the method still has its limitations, because the traditional method can only treat the genome information as a "black box", and the transmission information of alleles can only be determined by inference. Direct observations cannot be made, which may lead to inaccurate estimates of breeding values. With the development of high-throughput sequencing technology, it is entirely possible to obtain high-density SNP markers in animal...

AI Technical Summary

Problems solved by technology

Although the BLUP method has achieved great success in animal breeding, the method still has its limitations, because the traditional method can only treat the genome information as a "black box", and the transmission information of alleles can only be determined by inference. Impossible to make direct observations, which may lead to inaccurate estimates of breeding values

However, there is no report on genomic selective breeding for meat quality traits of large yellow croaker

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