Method for breeding raphanus sativus L. CMS (genic male sterility) lines by using marker assisted selection

A technology assisted by male sterile lines and molecular markers, applied in the fields of botanical equipment and methods, biochemical equipment and methods, applications, etc., can solve the problems of heavy workload and time-consuming screening and identification, and achieve high cost and high workload. Large, long-term effects

Inactive Publication Date: 2011-01-26
NANJING AGRICULTURAL UNIVERSITY
View PDF1 Cites 15 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to develop a method for assisted selection of radish candidate maintainer lines by using molecular markers in order to solve the problem of large workload and time-consuming screening and identification of candidate maintainer lines in the current

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for breeding raphanus sativus L. CMS (genic male sterility) lines by using marker assisted selection
  • Method for breeding raphanus sativus L. CMS (genic male sterility) lines by using marker assisted selection
  • Method for breeding raphanus sativus L. CMS (genic male sterility) lines by using marker assisted selection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0029] Implementation procedure of the present invention is:

[0030] (1) Selection of excellent inbred line materials

[0031] Select plots with good soil physical and chemical properties and drainage and irrigation conditions to plant different high-generation inbred lines of radish, and evaluate the main traits such as fleshy root shape, quality, plant resistance, and pollen fertility. Sexually stable inbred line.

[0032] (2) Genomic DNA of radish was extracted by the improved CTAB-chloroform-isoamyl alcohol method (Liu L, Guo W, Zhu X, Zhang T. Inheritance and fine mapping of fertility-restoration for cytoplasmic male sterility in Gossypium hirsutum L. Theor Appl Genet, 2003, 106:461-469).

[0033] (3) Identification of cytoplasmic sterility gene

[0034] Genomic DNA was extracted from radish inbred lines, inbred lines 1 to 16 were Nau-1, Nau-2, Nau-3, Nau-8, Nau-9, Nau-11, Nau-12, Nau-13, Nau-14, Nau-15, Nau-16, Nau-18, Nau-19, Nau-20, Nau-21, Nau-24 (Zhao-liang Lu, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for breeding raphanus sativus L. CMS (genic male sterility) lines by using marker assisted selection, which belongs to the technical field of crop breeding and is used for efficient breeding of excellent male sterility lines in raphanus sativus L. hybrid advantage utilization. The invention adopts the technical scheme that: the method comprises the following steps of: screening inbred lines with excellent traits and stable fertility; selecting specific primers NAUmsF4 and NAUmsR4 to perform PCR amplification on the screened inbred lines, and determining that the inbred lines capable of amplifying 1069bp specific strips cannot be used as candidate maintenance lines; performing restored gene functional marker analysis on the inbred lines incapable of amplifying the specific strips, and screening the inbred lines capable of serving as the candidate maintenance lines; and performing cross and back cross of 4 to 5 generations on the original sterile lines and the identified candidate maintenance lines to obtain new sterile lines and corresponding maintenance lines with excellent traits. The method for quickly screening the candidate maintenance lines by using molecular makers is not affected by a growth period and an environment, can remarkably improve the breeding efficiency of the raphanus sativus L. CMS lines, and accelerates the breeding progress of excellent F1 hybrids.

Description

technical field [0001] The invention discloses a method for cultivating radish CMS male sterile lines by using molecular marker-assisted selection, belongs to the technical field of crop breeding, and is used for efficient cultivation of excellent CMS male sterile lines in radish heterosis utilization. Background technique [0002] Radish (Raphanus sativus L.), also known as radish and reed, belongs to the genus Radish of Cruciferae. It is an important root vegetable crop native to my country. It has a long history of cultivation in my country, with various types and wide distribution; radish is mainly edible The part is a fleshy root, which is not only rich in nutrition, but also has high medicinal and therapeutic value, and is deeply loved by people (Edited by Wang Longzhi and He Qiwei, Chinese Radish, Science and Technology Literature Publishing House, 2005). [0003] Male sterility refers to the inability of plants to produce functional pollen or male gametes, and can be ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/68A01H1/02
Inventor 柳李旺孙新菊马二磊龚义勤汪良驹刘杨
Owner NANJING AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap