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New space-mutated rice grain length gene qGS3a and molecular marker method thereof

A molecular marker, rice technology, applied in the fields of biochemical equipment and methods, genetic engineering, plant genetic improvement, etc., can solve problems such as the limitations of molecular breeding and utilization, achieve good appearance quality and yield components, speed up the breeding process, save money. The effect of the process of de-adult stage grain identification

Inactive Publication Date: 2013-12-25
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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Problems solved by technology

However, the favorable alleles at these sites are all derived from natural mutations, so their application in molecular breeding has certain limitations.

Method used

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  • New space-mutated rice grain length gene qGS3a and molecular marker method thereof
  • New space-mutated rice grain length gene qGS3a and molecular marker method thereof
  • New space-mutated rice grain length gene qGS3a and molecular marker method thereof

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Experimental program
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Effect test

Embodiment Construction

[0020] The present invention will be further described below in conjunction with specific implementation examples. The methods used herein are conventional methods unless otherwise specified.

[0021] (1) Location of qGS3a gene

[0022] 1. Test materials

[0023] F 1 F 2 Progeny of 360 individual plants and their derived F 3 family lineage.

[0024] 2. DNA extraction, PCR amplification and gel electrophoresis

[0025] Referring to the DNA extraction method of Temnykh et al. (2000), the representative individual plants of each strain were mixed and extracted with genomic DNA. According to the reference map, 600 SSR markers evenly distributed in the whole genome were selected to synthesize primers. The genomic DNA of each strain was used as a template for polymerase chain reaction (PCR). The products of the PCR reactions were separated by polyacrylamide gel electrophoresis, stained with ethidium bromide, and imaged under a gel imaging system. With reference to the ampli...

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Abstract

The invention relates to a new gene qGS3a for simultaneously increasing rice grain length and grain weight and a molecular marker method thereof, belonging to the fields of rice high-yield good-quality breeding and molecular genetics. The invention is characterized in that single marker analysis is performed according to the linkage segregation law by using F2 population of large-grain mutant and original parent Nongken 58 and derivative F3 family to locate the new grain length gene qGS3a on the 3rd chromosome, thereby obtaining PCR (polymerase chain reaction) amplification-based practical and economical marker XM3-2 which is closely linked with the new qGS3a. The new gene qGS3a is used for auxiliary selective breeding and polymerization breeding of appearance quality and yield of rice, can effectively overcome the defects in spontaneous mutant genes, can perform genotype selection on the low-generation breeding population in the seedling stage to obtain the breeding material with better appearance quality and yield components, omits the grain identification process in the adult-plant stage, enhances the breeding efficiency and accelerates the breeding process.

Description

technical field [0001] The invention relates to a new gene qGS3a for increasing rice grain length and its molecular marker method, which belongs to the fields of rice high-yield and high-quality breeding and molecular genetics, and is suitable for introducing a new grain-length gene qGS3a in rice high-yield and high-quality breeding, and using molecular markers Assisted selection breeding and aggregation breeding for appearance quality and yield. Background technique [0002] Grain shape, especially grain length (GL), is an important component of rice appearance quality, while grain weight (TGW) is one of the important yield components. It is known that grain length and grain weight are affected by more than 100 genes / QTL loci, some of which have been finely mapped and / or cloned, mainly distributed on chromosome 2 (gw2), chromosome 3 (GS3 and GL3. 1), chromosome 5 (qSW5 / GW5), chromosome 7 (GS7 ​​and qSS7), and chromosome 8 (GW8). However, the favorable alleles at these sit...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12Q1/68
Inventor 郑天清黎志康徐建龙周永力赵秀琴王文生张帆孙勇王小倩
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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