SRAP molecular marker closely linked with male sterility genes of tomatoes and preparation method thereof

A technology of male sterility gene and molecular marker, which is applied in the field of agricultural biology, can solve the problems such as the long cycle of sterile line transfer, and achieve the effect of great significance in application and promotion, shorten the transfer cycle, and ensure the purity

Active Publication Date: 2014-10-29
HORTICULTURE INST OF XINJIANG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the general sterile line transfer cycle is very long, and it is possible to transfer

Method used

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  • SRAP molecular marker closely linked with male sterility genes of tomatoes and preparation method thereof
  • SRAP molecular marker closely linked with male sterility genes of tomatoes and preparation method thereof
  • SRAP molecular marker closely linked with male sterility genes of tomatoes and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0021] Embodiment 1, the SRAP molecular marker closely linked with tomato male sterility gene, described SRAP molecular marker is C10B9_1 and C10B9_4, and its primer combination is identical, is C10B9, and primer sequence is as follows:

[0022] C10B9 combined upstream primer C10: 5'-TGAGTCCAAACCGGCAT-'3, whose nucleotide sequence is shown in SEQ ID NO.1; downstream primer B9: 5'-GACTGCGTACGAATTCAG-'3, whose nucleotide sequence is shown in SEQ ID NO. .2 shown.

[0023] The linkage distances between the SRAP molecular markers C10B9_1 and C10B9_4 and the tomato male sterility gene ms are 3.3cM and -3.3cM, respectively.

Embodiment 2

[0024] Embodiment 2, the obtaining method of the SRAP molecular marker closely linked with tomato male sterility gene, described obtaining method comprises the following steps:

[0025](1) It is determined to use the purple-stem fertile inbred line 87-5 tomato as the male parent, and the green-stem male sterile tomato as the female parent, and the male sterile gene in the green-stem male sterile tomato is related to a seedling The green stem genes are tightly linked; the green stem male sterile tomato at the seedling stage is crossed with the purple stem fertile inbred line 87-5 tomato at the seedling stage to generate the F1 generation, and the F1 generation is selfed to generate the F2 segregation population, and then each F2 Genomic DNA of a single plant, for use;

[0026] (2) Select 10 green-stem sterile plants by the cluster analysis separation method from step (1) F2 isolation population, extract the genomic DNA of each strain, mix each strain of genomic DNA in equal amo...

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Abstract

The invention discloses an SRAP molecular marker closely linked with male sterility genes of tomatoes and a preparation method thereof. The method comprises the following steps: taking purple-stem fertile tomatoes 87-5 as male parents and green-stem male sterile tomatoes at the seedling stage as female parents, hybridizing to generate F1 generation, and selfing to build an F2 segregation population; performing SRAP marker analysis on male sterility genes ms by adopting a bulked segregant analysis method, randomly combining primers before and after SRAP, selecting 544 pairs of primer combinations, and screening between male sterile and fertile pools to obtain two polymorphic markers C10B9_1 and C10B9_4 between two DNA pools; performing SRAP marker verification on the F2 segregation population by virtue of the two markers, wherein linkage analysis discovers that the linkage distance between the marker C10B9_1 and the sterility genes ms and the linkage distance between the marker C10B9_4 and the sterility genes ms are 3.3cM and -3.3cM respectively. By virtue of the molecular marker, assisted selection of male sterile tomatoes can be performed, the transfer cycle is shortened, the transfer efficiency is improved, a tedious program of identifying the sterility of each generation by selfing in a transfer process is omitted, conventional phenotype selection is transformed into genotype selection, and the accuracy and scientificity of selection are improved.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to an SRAP molecular marker closely linked with a tomato male sterility gene and an obtaining method thereof. Background technique [0002] Tomato (Solanum lycopersicum L.) is a very widely cultivated vegetable all over the world, and it is also one of the main cultivated vegetables in my country. It can be eaten fresh or processed into different types of tomato products. Heterosis is a common phenomenon in the biological world, and it is an important way to improve crops and greatly increase yield. Tomato is a self-pollinated crop, with obvious heterosis. The yield of hybrids is 20-30% higher than that of ordinary varieties, and they have high uniformity and strong stress resistance. At present, the hybrids widely promoted in tomato production all use hybrid seed production. Still artificial emasculation is the main method, and the high cost of seed production...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 王强杨涛李宁唐亚萍王柏柯杨生保帕提古丽·艾斯木托拉余庆辉
Owner HORTICULTURE INST OF XINJIANG ACAD OF AGRI SCI
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