3 results about "Dna pooling" patented technology

The invention discloses a method for rapidly identifying common cattle and tumor cattle by using a deleted large-fragment rapid structure variation marker in a tumor cattle LRP5 gene and an application of the method. Specific high-frequency SV of the common cattle and the tumor cattle is identified through whole genome data analysis. Based on a PCR (Polymerase Chain Reaction) technology, a blood genome DNA (Deoxyribonucleic Acid) pool of Xinjiang brown cattle and Wenshan cattle is used as a template, specific upstream and downstream primers are used for amplifying structural variation of large fragment deletion of cattle LRP5 genes, then agarose gel electrophoresis is carried out, and different individuals are divided into a non-deletion type, a deletion type and a heterozygous type according to electrophoresis results. A correlation analysis result shows that different genotypes of the LRP5 gene are remarkably related to the cattle variety, so that the detection method disclosed by the invention can be applied to variety identification and marker-assisted selection breeding of common cattle and tumor cattle, an excellent cattle genetic resource group is quickly established, and the breeding cost is reduced.

The application provides a molecular marker related to sheep immunity traits and application thereof. The application uses a method for detecting a PLEKHH2 gene SNP site of Lake sheep by combining DNA pool sequencing with KASPar technology. A primer is designed according to a PLEKHH2 gene sequence, DNA is extracted from sheep blood, and PCR amplification, DNA sequencing and sequence analysis are carried out, so that a C / G polymorphic site at the 368th position of the amplified fragment is found. Further, KASPar primers are used to detect the polymorphic site of 889 Lake sheep and to establish a least square model, and correlation analysis is carried out on the genotype and immunity trait indexes, so that it is found that the site is significantly related to the platelet count (PLT) and the mean corpuscular hemoglobin concentration (MCHC) of sheep. Through detection of the molecular marker of the application, the application can be used in marker-assisted disease-resistant breeding of sheep and can accelerate the breeding process of sheep.

The invention belongs to the technical field of biology, and particularly relates to an efficient prawn DNA mixed pool construction method and breeding application. The method specifically comprises the following steps: taking prawn tails of prawns as tissues of equivalent mixed single individuals, taking one prawn tails of each to-be-treated individual, merging and mixing the taken prawn tails, cutting the same length to realize uniform mixing of the equivalent tissues of the individuals, and then extracting DNA of the mixed tissues, namely constructing a DNA mixed pool. The technical scheme provided by the invention effectively solves the problems of difficulty in accurate quantification, complex operation and high cost in construction of a large number of DNA mixed pool tissues in traditional BSA analysis, simplifies the identification process of the character-related markers, is suitable for genetic analysis of large-scale prawn samples and identification of the character-related markers, and has a wide application prospect in the field of prawn breeding.