31 results about "Dna pooling" patented technology

The invention provides DNA aptamer sequences for recognizing bentazone and derivatives thereof. DNA aptamer as referred in the invention is single-stranded oligodeoxynucleotide with different oligodeoxynucleotide sequences; and the sequences are capable of recognizing the herbicide bentazone in a solution and applicable as biological recognition materials for detection of the content of bentazone in samples. The DNA aptamer sequences are obtained through in-vitro seletion or SELEX from a random DNA pool. In an aqueous solution, the bonding strength of the DNA aptamer sequences and bentazone molecules dissociation constant enables a dissociation constant (Kd) to reach the magnitude of [mu]M.

The invention discloses an SRAP molecular marker closely linked with male sterility genes of tomatoes and a preparation method thereof. The method comprises the following steps: taking purple-stem fertile tomatoes 87-5 as male parents and green-stem male sterile tomatoes at the seedling stage as female parents, hybridizing to generate F1 generation, and selfing to build an F2 segregation population; performing SRAP marker analysis on male sterility genes ms by adopting a bulked segregant analysis method, randomly combining primers before and after SRAP, selecting 544 pairs of primer combinations, and screening between male sterile and fertile pools to obtain two polymorphic markers C10B9_1 and C10B9_4 between two DNA pools; performing SRAP marker verification on the F2 segregation population by virtue of the two markers, wherein linkage analysis discovers that the linkage distance between the marker C10B9_1 and the sterility genes ms and the linkage distance between the marker C10B9_4 and the sterility genes ms are 3.3cM and -3.3cM respectively. By virtue of the molecular marker, assisted selection of male sterile tomatoes can be performed, the transfer cycle is shortened, the transfer efficiency is improved, a tedious program of identifying the sterility of each generation by selfing in a transfer process is omitted, conventional phenotype selection is transformed into genotype selection, and the accuracy and scientificity of selection are improved.

The invention discloses a detection method of Chinese simmental cattle carcass and meat quality trait genetic marker, and the method uses DNA pool sequencing and PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) technology for screening and detection of GPAM (glycerol-3-phosphate acyltransferase, mitochondrial) gene function mutation of Chinese simmental cattle. Through the correlation analysis, Chinese simmental cattle carcass and meat quality trait-related SNPs as a molecular genetic marker is found, and is used in the early selection and molecular pyramiding breeding of beef cattle, and breeding and improving of meat use performance of the Chinese simmental cattle can be further accelerated.

The invention relates to a gene ZmSmk3 for encoding corn mTERF protein and a cloning method for the gene ZmSmk3. The cloning method comprises the following steps of: respectively extracting DNA of a plurality of homozygous mutants and homozygous wild type plant leaves, mixing the extracted DNA of the homozygous mutants and constructing a DNA pool of the homozygous mutants; mixing the extracted wild type DNA and constructing a mild type DNA pool; respectively carrying out heat-asymmetric staggering PCR (Polymerase Chain Reaction) on the constructed DNA pool of the homozygous mutants and the wild type DNA pool to obtain PCR product segments; detecting obtained PCR amplification product segments by using agarose gel electrophoresis, recovering specific bands generated in the DNA pool of the homozygous mutants, and carrying out sequencing to obtain a sequence of the mutant gene ZmSmk3. The mutation of the gene ZmSmk3 for encoding the corn mTERF protein has an influence on the seed development progress, and the gene has an important guidance significance for breeding practice.

The invention discloses a SNP molecular marker in a porcine SNCG gene for tracing and a detection method thereof. The SNP molecular marker is obtained by DNA pool sequencing and has altogether 1599 bp. The SNP molecular marker contains a part of the sequence of a third exon; a base at a position 984 mutates from 984C to 984T; the DNA sequence of the SNP molecular marker is represented by SEQ ID NO. 1. The results of analysis of the allelic distribution of the SNP molecular marker in a trial group containing 11 pig species / strains show that allelic frequencies of the SNP molecular marker in different species or strains are approximate, polymorphism is rich, allelic frequency distribution difference between species or strains is small, the degree of heterozygosis is all larger than 0.3, and thus, the SNP molecular marker disclosed herein can be used for DNA tracing for pork products.

The invention provides a gene positioning method of brassica napus wax powder based on whole genome resequencing, which belongs to the technical field of biological information. The present invention establishes near-isogenic lines on the basis of segregating populations. Using mixed grouping analysis, non-wax plants in the 3:1 segregation population of near isogenic lines, all non-segregation populations with wax phenotype and 3 parents were selected, and 5 DNA pools were constructed for genome resequencing. The resequencing data was used for genetic association analysis, and the loci controlling the traits of wax powder were located in the 590663-1657546bp region of the A08 chromosome. The biggest feature of the method of the present invention is that it does not need to detect all progeny individuals, but conducts mixed pool analysis on the progeny of two extreme traits. Moreover, the sequencing data of the method of the present invention are sufficient and of acceptable quality, and can be used for the next step of analysis.

The invention discloses a method for detecting Indel markers of beef cattle PLAG1 genes and a special kit thereof. The method comprises the following steps: based on a PCR (Polymerase Chain Reaction)technology, by taking a blood genome DNA pool of beef cattle (Jiaxian red cattle) as a template, and amplifying Indel sites of cattle PLAG1 genes by utilizing a specific primer pair P1; and performingagarose gel electrophoresis, and dividing different individuals into a deletion form, a normal form and a hybrid form according to electrophoresis results. The method provided by the invention is established on a relevance basis between the Indel sites of beef cattle PLAG1 genes and growth traits, and the method is simple, rapid and convenient to popularize and use and contributes to acceleratingmolecular marker-assisted selection of the beef cattle.

The invention discloses an SNP molecular marker in a porcine AMY2 gene for tracing and a detection method thereof. The SNP molecular marker is obtained through DNA pool sequencing and has altogether 1060 bp. The SNP molecular marker contains a part of the sequence of a fifth exon, a part of the sequence of a seventh exon and the complete sequences of a fifth intron, a sixth exon and a sixth intron; a base at a position 104 mutates from 104 C to 104 T; the DNA sequence of the SNP molecular marker is represented by SEQ ID NO. 1, and an amino acid sequence encoded by the SNP molecular marker is represented by SEQ ID NO. 2. The SNP molecular marker is used in cultivation of commercial pigs and is extensively applicable to DNA tracing and detection for pork products, especially to security tracing for pork products.

A method for screening pathogenic genes with high contribution to rheumatoid arthritis, comprising the following steps: setting a rheumatoid arthritis patient group and a healthy control group, each group including several peripheral blood samples from different human bodies that have been separated from the human body; extracting DNA in peripheral blood samples; prepare the DNA pool of the rheumatoid arthritis patient group and the DNA pool of the healthy control group; measure the content, quality and integrity of the DNA pool, and if it is available, proceed to the next step; construct a DNA library; The genome resequencing method is used to sequence the rheumatoid arthritis patient group and the healthy control group respectively to obtain the sequencing results of the rheumatoid arthritis patient group and the healthy control group; the sequencing results of the rheumatoid arthritis patient group Compared with the sequencing results of the healthy control group, high-contributing gene variation sites were screened out. The above-mentioned screening method can screen out the mutation sites of highly contributing pathogenic genes, which is beneficial to the early treatment and prevention of the inflammation.

The invention belongs to the technical field of bioengineering, in particular to a breeding method for high-yielding Jersey cows. In this application, 250 10-base random primers were used to perform RAPD-PCR between two DNA pools composed of 20 high-yield (305d milk yield>4000kg) and 20 low-yield (305d milk yield<3200kg) Jersey cattle. Amplified molecular marker screening method. All individuals were tested by PCR with 200 primers screened, and three RAPD markers related to milk production traits of dairy cows were obtained, and they were cloned, sequenced and analyzed by bioinformatics. The test results showed that the DNA fragments of S11, S1110 and S1120 were related to milk yield, among which S11 was positively correlated with milk yield traits, and S1110 and S1120 were negatively correlated with milk yield traits.