Screening method for highly contributing pathogenic genes in rheumatoid arthritis

Abstract

A method for screening pathogenic genes with high contribution to rheumatoid arthritis, comprising the following steps: setting a rheumatoid arthritis patient group and a healthy control group, each group including several peripheral blood samples from different human bodies that have been separated from the human body; extracting DNA in peripheral blood samples; prepare the DNA pool of the rheumatoid arthritis patient group and the DNA pool of the healthy control group; measure the content, quality and integrity of the DNA pool, and if it is available, proceed to the next step; construct a DNA library; The genome resequencing method is used to sequence the rheumatoid arthritis patient group and the healthy control group respectively to obtain the sequencing results of the rheumatoid arthritis patient group and the healthy control group; the sequencing results of the rheumatoid arthritis patient group Compared with the sequencing results of the healthy control group, high-contributing gene variation sites were screened out. The above-mentioned screening method can screen out the mutation sites of highly contributing pathogenic genes, which is beneficial to the early treatment and prevention of the inflammation.

Description

technical field [0001] The invention relates to a method for obtaining high-contribution pathogenic gene information of rheumatoid arthritis as an intermediate result, in particular to a screening method for high-contribution pathogenic genes of rheumatoid arthritis. Background technique [0002] Rheumatoid Arthritis (RA) is an autoimmune disease with a high incidence in the world. Among them, the incidence in my country remains high, and the incidence of women is much higher than that of men. It has always been the biggest and most common problem that plagues people's normal life. It is one of the most common diseases, which is characterized by joint symmetry and destruction, eroding the bones and joints of the human body, mild cases of temporary pain, inconvenience of movement, inflexibility of limbs, etc., severe cases of walking difficulties, skeletal deformities, It is accompanied by painful and irreversible pathological features, and causes multiple inflammations of vari...

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Problems solved by technology

[0003] Rheumatoid arthritis has an inseparable relationship with microbial infection. Any bacterial invasion and infection will aggravate the pathological changes of rheumatoid arthritis. This may be caused by the secretion of certain substances after the invasion of bacteria that changes the protein structure. In this case, in recent years, we have extracted a large number of viruses such as herpes virus, staphylococcus, and zoster virus in the joints of patients with rheumatoid arthritis, which has attracted enough attention, and we have initially concluded that some bacteria and viruses can cause, Changing the pathological characteristics of rheumatoid arthritis and exacerbating the deterioration of the disease, not only that, a sampling survey experiment was carried out in the family of rheumatoid arthritis patients with obvious family genetic tendency, and it was found that the incidence of rheumatoid arthritis is high The gene is located in the 21-23 section of human chromosome 6, and there is a very important pathogenic factor called rheumatoid factor, which can be recognized by a protein antibody secreted by B cells to form a complex complement , if it enters the joints, it will be hydrolyzed by enzymes, and then destroy the joint organs to form the initial pathology of symptoms, but later found that the occurrence of this pathology is common between patients and healthy patients, so it cannot be included in the disease The other is that the DNA genome of patients with rheumatoid arthritis has obvious methylation after research, resulting in abnormal expression of many genes, which can affect the occurrence of joints in the body organs. The theory of rheumatoid arthritis has also produced several influential theories. It is believed that people with HLA-DR in different people's bodies have a stronger tendency to develop the disease, mainly because there are similar genes in this gene. The amino acid sequence is used as the receptor of the surface response mechanism, and the other is the elaboration of the LMP theory. The LMP-2 and 7 genes have polymorphisms, which are related to many diseases. Through the genetic variation of the LMP site, it is speculated that it is possible Linked to the pathogenesis of RA

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