Detection method of Chinese simmental cattle carcass and meat quality trait genetic markers

Abstract

The invention discloses a detection method of Chinese simmental cattle carcass and meat quality trait genetic marker, and the method uses DNA pool sequencing and PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) technology for screening and detection of GPAM (glycerol-3-phosphate acyltransferase, mitochondrial) gene function mutation of Chinese simmental cattle. Through the correlation analysis, Chinese simmental cattle carcass and meat quality trait-related SNPs as a molecular genetic marker is found, and is used in the early selection and molecular pyramiding breeding of beef cattle, and breeding and improving of meat use performance of the Chinese simmental cattle can be further accelerated.

Description

technical field

[0001] The invention discloses a method for detecting genetic markers of Chinese Simmental cattle carcass and meat quality traits, and belongs to the technical field of genetic engineering detection of domestic carnivorous animals. Background technique

[0002] Chinese Simmental is a large-scale breed of milk and meat service that was independently bred in my country after it was introduced in the 1950s. Because of its strong physique, strong adaptability, and excellent milk, meat and service performance, it has become the most domestically raised and widely distributed breed. The most extensive variety is the leading variety in my country's beef cattle industry. With the changes in domestic and foreign markets and the increase in consumer demand for beef, further breeding and improvement of the performance of Chinese Simmental beef has become the main goal of this breed. In order to improve meat performance, conventional breeding techniques and molecular bre...

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